Analysis of User’s Hair Cannabinoid of Narcotic Type of Marijuana (Cannabis Sativa L.) Using GCMS Technic
Muhammad Taufik,
Harlem Marpaung,
Jamaran Kaban,
Basuki Wirjosentono
Issue:
Volume 4, Issue 1, February 2016
Pages:
1-10
Received:
14 February 2016
Accepted:
21 February 2016
Published:
9 March 2016
Abstract: This paper describes the analysis of cannabinoids in the hair of the user of the narcotic type of marijuana (Cannabis sativa L), using the technique Gas Chromatography Mass Spectroscopy (GCMS) and the validation of the method used. This research was conducted through experiments. Samples were taken from users of narcotic type of marijuana, found in Al-Kamal Sibolangit Rehabilitation Centre, North Sumatra, Indonesia. Marijuana plants were taken from the evidence in Forensic Laboratory of Medan Branch Police. Extraction is done through maceration by sonication at 42 KHz and using methanol – 2 propanol (1:1), kloroform, and petroleum benzene each of 2 ml for 5 minutes. Testing is done with fast blue salt B test and show there is a precipitate Violet. Optimal results obtained at a temperature of 50°C extraction. Identification is done with Thin Layer Chromatography (TLC) in the mobile phase of toluene and n-hexane (1:1) under alkaline conditions of pH 9,5, followed by GC MS techniques for sixteen (16) minutes. The results are contained compounds of cannabidiol, cannabinol, and Δ9THC (Tetrahydrocannabinoid) with concentrations ranging from 0.25 up to 2.82 ng/mg in the hair of the user. Validation of GC MS method for the compounds of cannabidiol, cannabinol, and Δ9THC produces an accuracy value with %recovery is 103,83, 102,67, and 101,17 respectively. Precision test produces a value of Relatif Standard Deviation (RSD) = 1,3058%, 0,8997%, dan 0,8997% respectively. Linearity test generate r value each of the regression is 0,922, 0,955, and 0,921. Limits of Detection (LOD) is obtained 0,000168 ng/mg, 0,000117 ng/mg, and 0,000164 respectively. Limits of Quantification (LOQ) is 0,00056 ng/mg, 0,00039 ng/mg, and 0,00054 ng/mg respectively. This indicates that the modification process of extraction and GC MS techniques used could produce cannabinoid compounds (cannabidiol, cannabinol, and Δ9THC) in hair samples quickly and accurately.
Abstract: This paper describes the analysis of cannabinoids in the hair of the user of the narcotic type of marijuana (Cannabis sativa L), using the technique Gas Chromatography Mass Spectroscopy (GCMS) and the validation of the method used. This research was conducted through experiments. Samples were taken from users of narcotic type of marijuana, found in...
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