Retrospective Analysis of Bacterial Pathogens Isolated from Wound Infections at a Tertiary Hospital in Nguru, Yobe State Nigeria
Bularafa Mohammed Yasidi,
Denue Ballah Akawu,
Onah Joseph Oihoma,
Jibrin Yusuf Bara,
Umar Hamzat Mohammed,
Gabchiya Nguru Mohammed,
Zanna Baba Ali,
Ladan Joshua,
Hamidu Ibrahim,
Okon Kenneth Okwong
Issue:
Volume 3, Issue 1, February 2015
Pages:
1-6
Received:
6 March 2015
Accepted:
20 March 2015
Published:
24 March 2015
Abstract: Wound infections inflict clinical and societal consequences on the patients, but its bacteriological characteristic varies with different factors. Therefore, effective treatment and management of wound infections in hospital and community setting will require detailed epidemiological knowledge of the infecting bacterial pathogens and their antibiogram peculiar to the environment. Based on this information, we examined the prevalence and antibiogram of bacterial pathogens isolated from wound infection cases seen at the hospital over the study period. A total of 392 wound swabs/ and pus of different types of wound infections from different anatomical sites and associated clinical conditions were analyzed by standard bacteriological methods. Of the 392 clinical specimens analyzed, 301(76.8%) yielded at least one bacterial pathogen, 25(6.4%) polymicrobial, no anaerobes identified and 91(23.2%) yielded no bacterial growth, gender distribution, 204(67.8%) males and 97(32.2%) females, and majority of pathogens were recovered from septic wound infections. Overall, 7 different bacterial pathogens were identified 5(71.4%) gram-negative bacteria isolates and 2(28.6%) gram-positive bacterial isolates]. Staphylococcus aureus accounted for majority of the bacterial pathogens isolated, 162(53%) followed by coliforms 62(21%) and Pseudomonas aeroginosa 57(19%). The bacterial pathogens demonstrated high resistance to ampicillin(78%), amoxicillin(66%), and cotrimoxazole(78%), in contrast to high sensitivity pattern observed with fluoroquinolones ( ofloxacin 83%,, norfloxacin 71%, ciprofloxacin 78%), erythromycin 72%, chloramphenicol 62%, gentamycin 58% and ceftazidime 60%. The relatively high number of wound infection cases seen within the study period is of public health concern, while the low number of bacterial pathogens isolated underscores the need for improvement in the laboratory diagnostic approach for effective treatment and management of wound infections.
Abstract: Wound infections inflict clinical and societal consequences on the patients, but its bacteriological characteristic varies with different factors. Therefore, effective treatment and management of wound infections in hospital and community setting will require detailed epidemiological knowledge of the infecting bacterial pathogens and their antibiog...
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Isolation and Characterization of Diamine Oxidase Enzyme from Mung Bean Sprouts (Vigna radiata L)
Abdul Karim,
Abd Rauf Patong,
Abd Wahid Wahab,
Indah Raya
Issue:
Volume 3, Issue 1, February 2015
Pages:
7-11
Received:
9 March 2015
Accepted:
22 March 2015
Published:
26 March 2015
Abstract: The diamine oxidase enzyme (DAO) can be isolated from mung bean sprouts (Vigna radiata L) through the process of extraction in phosphate buffer. Concentration and fractionation were performed by the addition of ammonium sulphate, (NH4)2SO4 saturation in stages and cold centrifuged at 10000 rpm. Enzyme activity of DAO crude and DAO fractionation results were tested using substrat histamin and measured with a spectrophotometer at a wavelength of 450 nm. This study revealed that the enzyme activity of the crude enzyme was1, 226 mU/mL, the fraction of 40-60% ammonium sulphate saturated had the highest activity which accounted for 3326 mU/mL and after gel filtration, the results for both activity and specific activity were 116 mU/mL and 185 mU/mg protein.
Abstract: The diamine oxidase enzyme (DAO) can be isolated from mung bean sprouts (Vigna radiata L) through the process of extraction in phosphate buffer. Concentration and fractionation were performed by the addition of ammonium sulphate, (NH4)2SO4 saturation in stages and cold centrifuged at 10000 rpm. Enzyme activity of DAO crude and DAO fractionation res...
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