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Comparative Study on Prion Protein Detection Methods Using Biosensor Technology for Beef and Its Edible Products
Kazi Naziba Tahsin,
Rakib Hasan,
Shahriar Khan
Issue:
Volume 6, Issue 1, February 2018
Pages:
1-8
Received:
23 November 2017
Accepted:
29 November 2017
Published:
17 January 2018
Abstract: Prion diseases in humans are closely linked to consumption of bovine meant or organs infected with abnormal prions. Bovine organs which are most prone to prion disorders such as the brain and the liver, kidney etc are considered in various traditional cuisines over the world. Meat or edible parts should be tested safe from prions before releasing it into the market. The detection of prions proteins in bovine meat and other organs is a challenging task. The renowned methods include Quartz Crystal Microbalance and Surface Plasmon Resonance techniques. This paper narrates a performance analysis of the accuracy, sensitivity and feasibility of each technique over the other for the detection of abnormal prion protein. Successful detection of the infected prions will reduce the diseases related to abnormal prions in entering the food chain.
Abstract: Prion diseases in humans are closely linked to consumption of bovine meant or organs infected with abnormal prions. Bovine organs which are most prone to prion disorders such as the brain and the liver, kidney etc are considered in various traditional cuisines over the world. Meat or edible parts should be tested safe from prions before releasing i...
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Regulation of Bone Health Parameters in MG-63 Cell Line After Treatment with Biofield Energy Treated Vitamin D3
Matthew Charles Hornung,
Mahendra Kumar Trivedi,
Alice Branton,
Dahryn Trivedi,
Gopal Nayak,
Mayank Gangwar,
Snehasis Jana
Issue:
Volume 6, Issue 1, February 2018
Pages:
9-16
Received:
21 January 2018
Accepted:
1 February 2018
Published:
2 March 2018
Abstract: The present experiment aimed to explore the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test items received the Consciousness Energy Healing Treatment by Matthew Charles Hornung and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of both the sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability in tested samples was more than 88% on MG-63 cell line. ALP level was significantly increased by 273.3% (at 0.1 µg/mL), 694.1% (at 1 µg/mL), and 180% (at 0.1 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 105.8% (at 1 µg/mL), 164.6% (at 0.1 µg/mL), and 193.8% (at 0.1 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Bone mineralization percentage was significantly increased by 238.1%, 92.7%, and 43.1% at 0.1, 1, and 10 µg/mL, respectively in UT-DMEM+BT-TI group, while 297.6%, 86.2%, and 66.3% at 0.1, 1, and 10 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 281%, 113%, and 47% at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.
Abstract: The present experiment aimed to explore the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test items received...
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Birth Preparedness and Complication Readiness Among Pokot Nomadic Pastoralists’ Pregnant Women in East Pokot District, Midwest- Kenya
Kiptulon Evans Kasmai,
Onoja Mathew Akpa,
Oladapo Olayemi
Issue:
Volume 6, Issue 1, February 2018
Pages:
17-23
Received:
1 March 2018
Accepted:
14 March 2018
Published:
8 April 2018
Abstract: Birth Preparedness and Complication Readiness (BPCR) is a strategy to promote the timely use of skilled maternal and neonatal care, especially during childbirth, based on the theory that preparing for childbirth and being ready for complications reduces delays in seeking health care and averts serious complications that women face during child birth. However, information on BPCR among Pokot nomadic pastoralists have not been well documented in literatures. The purpose of this research was to determine factors affecting BPCR among Pokot nomadic pastoralists’ pregnant women living in East Pokot District of Kenya. A cross-sectional facility based study was conducted among 275 Pokot pregnant women, above 18 years of age who were on their second and third trimesters and attending various Antenatal Clinics (ANCs) in East Pokot district. This was response rate of 90.1%. A multi-stage sampling technique was used and data was collected from those who met the inclusion criteria between August-October, 2017, using a pretested researcher administered questionnaire. The data collected was analyzed using (SPSS) version 21. Chi-square was used to test the hypothesis. A p-value of <0.05 was considered significant. The results showed that practice of BPCR was low (28%) among the respondents. Being educated (OR=18, CI=3.83-85.16) and having high household income (OR=2.53, CI=1.37-4.66) increased practice of BPC R. Ethnic affiliation to Pokot (OR=4.85, CI=1.38-17.07), practicing African Traditional Religion (OR=2.48, CI=1.44-4.26), practicing pastoralism (OR=2, CI=1.17-3.42) were found to significantly reduce the level of BPCR. In conclusion, birth preparedness and complication readiness was low among Pokot nomadic pastoralist’s pregnant women. There is a need for more BPCR awareness programmes targeting pastoralists’ women and other vulnerable groups in the region.
Abstract: Birth Preparedness and Complication Readiness (BPCR) is a strategy to promote the timely use of skilled maternal and neonatal care, especially during childbirth, based on the theory that preparing for childbirth and being ready for complications reduces delays in seeking health care and averts serious complications that women face during child birt...
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Regulation of Bone Health Parameters After Treatment with Biofield Energy Healing Based Vitamin D3 on Human Osteoblast Cell Line (MG-63)
Victoria Lee Vannes,
Mahendra Kumar Trivedi,
Alice Branton,
Dahryn Trivedi,
Gopal Nayak,
Mayank Gangwar,
Snehasis Jana
Issue:
Volume 6, Issue 1, February 2018
Pages:
24-32
Received:
28 November 2017
Accepted:
7 December 2017
Published:
20 April 2018
Abstract: Bone disorders dramatically affecting the functional status of many individuals, which are suffering from bone diseases such as fractures, significant pain and height loss, disability to stand up, and walk. Vitamin D play an important role to improve the patients' quality of life with respect to bone disorders. The current study aimed to evaluate the potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 and DMEM as test item (TI) on bone cell differentiation using human osteoblast cell line (MG-63, Osteosarcoma). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were evaluated. The test items were treated with The Trivedi Effect® by Victoria Lee Vannes and divided as Biofield Energy Treated (BT) and untreated (UT) test items. Cell viability using MTT data showed that the test items were found to be safe. ALP level was significantly increased by 114.3%, 304.8%, and 314.3% at 0.1 µg/mL in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared to the untreated group. Collagen content was significantly increased by 82.5%, 138.4%, and 100.8% at 0.1, 1, and 10 µg/mL, respectively in the UT-DMEM+BT-TI, while 120.6% and 64.6% increased collagen at 0.1 and 1 µg/mL in BT-DMEM+UT-TI group and 261.9%, 179.8%, and 116.0% increased collagen in BT-DMEM+BT-TI group at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 261.2% and 239.9% at 1 µg/mL UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively as compared with the untreated group. However, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 324.5% at 50 µg/mL. Thus, Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.
Abstract: Bone disorders dramatically affecting the functional status of many individuals, which are suffering from bone diseases such as fractures, significant pain and height loss, disability to stand up, and walk. Vitamin D play an important role to improve the patients' quality of life with respect to bone disorders. The current study aimed to evaluate t...
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