Evaluation of the Energy of Consciousness Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy
Mahendra Kumar Trivedi,
Alice Branton,
Dahryn Trivedi,
Gopal Nayak,
Alan Joseph Balmer,
Dimitrius Anagnos,
Janice Patricia Kinney,
Joni Marie Holling,
Joy Angevin Balmer,
Lauree Ann Duprey-Reed,
Vaibhav Rajan Parulkar,
Parthasarathi Panda,
Kalyan Kumar Sethi,
Snehasis Jana
Issue:
Volume 5, Issue 2, April 2017
Pages:
16-25
Received:
27 February 2017
Accepted:
8 March 2017
Published:
31 March 2017
Abstract: Withania somnifera (Ashwagandha) root extract is very useful herbal medicine since from ancient time. The current study designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural properties of the ashwagandha root hydroalcoholic extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while other part was treated with the Energy of Consciousness Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time of the phytoconstituents remained same in both the control and treated samples, whereas the peak area % at respective retention time was significantly altered. The peak area% of the treated sample at Rt of 5.3, 5.5, 6.4, 6.5, 6.8, 6.9, 7.1, 7.3, 7.8, 7.9, 8.0, 8.2, 8.4, 8.6, 8.8, 9.0, 9.1, and 10.1 minutes were significantly reduced by 0.57% to 38.10% compared to the control sample. In addition, the peak area% of the treated sample at Rt of 5.7, 6.7, and 8.1 minutes were significantly increased by 16.00%, 244.44% and 19.62%, respectively compared with the control sample. A total of 21 withanolides such as withanoside IV, coagulin Q, viscosa lactone B, withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A and withanolide sulfoxide, ixocarpalactone A, withanolide sulfoxide, withanolide B, etc. were proposed with their structure from the molecular mass at m/z 783, 569, 621, 489, 473, 767, 471, 505, 992, and 455 at retention times of 6.4, 6.5, 6.8, 7.1, 7.9, 8.1, 8.4, 9.1, and 10.1 minutes with the help of GC-MS and NMR data of both the control and Biofield Energy Treated samples. There were significant changes observed in the peak intensity values at the same retention time in the Biofield Energy Treated sample in the range of -75.32% to 108.51% compared with the control sample. These findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of W. somnifera extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.
Abstract: Withania somnifera (Ashwagandha) root extract is very useful herbal medicine since from ancient time. The current study designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural properties of the ashwagandha root hydroalcoholic extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandh...
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Impact of Consciousness Energy Healing Treatment on Herbomineral Formulation in Different Skin Health Parameters
Deborah Lea Smith,
Mahendra Kumar Trivedi,
Alice Branton,
Dahryn Trivedi,
Gopal Nayak,
Sambhu Charan Mondal,
Snehasis Jana
Issue:
Volume 5, Issue 2, April 2017
Pages:
26-35
Received:
27 March 2017
Accepted:
19 April 2017
Published:
3 May 2017
Abstract: The aim of this study was to evaluate the effect of the Consciousness Energy Healing based test formulation and cell medium (DMEM) against various skin health parameters. The test formulation and DMEM were divided into two parts. One part received the Consciousness Energy Healing Treatment (The Trivedi Effect®) by Deborah Lea Smith and termed as the Biofield Energy Treated sample, while other part denoted as untreated test item. The study was assessed for the measurement of collagen, elastin, hyaluronic acid, melanin synthesis, cell viability against UV-B induced stress, and wound healing activity. MTT assay showed that the test formulation was safe and nontoxic in three cell lines. Bromodeoxyuridine (BrdU) assay data showed that the percent cell proliferation was increased significantly by 22.69% and 34.99% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. Collagen was increased by 15.12% and 15.26% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. Elastin was significantly increased by 9.10%, 13.71%, and 12.41% in the UT-DMEM + BT-Test formulation, BT-DMEM + Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 5 µg/mL compared to the untreated group. Elastin was also increased significantly by 17.78% and 6.01% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. Hyaluronic acid was significantly increased by 3.90%, 5.37%, and 6.82% in the UT-DMEM + BT-Test formulation at 0.625, 1.25, and 2.5 µg/mL, respectively compared to the untreated group. Melanin level was reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the untreated group. Skin cells protection was significantly increased by 21.32%, 23.81%, and 7.20% at 0.625, 1.25, and 2.5 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared to the untreated group. Wound healing assay exhibited significant wound closure and cell migration in all the tested groups compared to the untreated group. Overall, the Biofield Energy Treated test formulation and DMEM (The Trivedi Effect®) were found to be safe and can be developed as an effective cosmetic product to protect and treat the skin problems including infection, photosensitivity, acne, hives, chickenpox, eczema, rosacea, seborrheic dermatitis, psoriasis, erythema, contact dermatitis, skin aging, wrinkles, etc.
Abstract: The aim of this study was to evaluate the effect of the Consciousness Energy Healing based test formulation and cell medium (DMEM) against various skin health parameters. The test formulation and DMEM were divided into two parts. One part received the Consciousness Energy Healing Treatment (The Trivedi Effect®) by Deborah Lea Smith and termed as th...
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