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Analysis of User’s Hair Cannabinoid of Narcotic Type of Marijuana (Cannabis Sativa L.) Using GCMS Technic

Received: 14 February 2016     Accepted: 21 February 2016     Published: 9 March 2016
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Abstract

This paper describes the analysis of cannabinoids in the hair of the user of the narcotic type of marijuana (Cannabis sativa L), using the technique Gas Chromatography Mass Spectroscopy (GCMS) and the validation of the method used. This research was conducted through experiments. Samples were taken from users of narcotic type of marijuana, found in Al-Kamal Sibolangit Rehabilitation Centre, North Sumatra, Indonesia. Marijuana plants were taken from the evidence in Forensic Laboratory of Medan Branch Police. Extraction is done through maceration by sonication at 42 KHz and using methanol – 2 propanol (1:1), kloroform, and petroleum benzene each of 2 ml for 5 minutes. Testing is done with fast blue salt B test and show there is a precipitate Violet. Optimal results obtained at a temperature of 50°C extraction. Identification is done with Thin Layer Chromatography (TLC) in the mobile phase of toluene and n-hexane (1:1) under alkaline conditions of pH 9,5, followed by GC MS techniques for sixteen (16) minutes. The results are contained compounds of cannabidiol, cannabinol, and Δ9THC (Tetrahydrocannabinoid) with concentrations ranging from 0.25 up to 2.82 ng/mg in the hair of the user. Validation of GC MS method for the compounds of cannabidiol, cannabinol, and Δ9THC produces an accuracy value with %recovery is 103,83, 102,67, and 101,17 respectively. Precision test produces a value of Relatif Standard Deviation (RSD) = 1,3058%, 0,8997%, dan 0,8997% respectively. Linearity test generate r value each of the regression is 0,922, 0,955, and 0,921. Limits of Detection (LOD) is obtained 0,000168 ng/mg, 0,000117 ng/mg, and 0,000164 respectively. Limits of Quantification (LOQ) is 0,00056 ng/mg, 0,00039 ng/mg, and 0,00054 ng/mg respectively. This indicates that the modification process of extraction and GC MS techniques used could produce cannabinoid compounds (cannabidiol, cannabinol, and Δ9THC) in hair samples quickly and accurately.

Published in American Journal of Biomedical and Life Sciences (Volume 4, Issue 1)
DOI 10.11648/j.ajbls.20160401.11
Page(s) 1-10
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Analiysis, Hair, Marijuana (Cannabis sativa L), GCMS, KLT, Validation

References
[1] Jakubovic, A., McGeer, E. G., & MsGeer, P. L. (1981). Effect of Cannabinoids and Narcotics on Gonodal Functions. Neuroend ocrine Regulation and Altered behavior, 151-168. Doi. 10.100 7/978-1-4684-4067-6_7.
[2] Onaivi, E. S. (2006). Marijuana and Canabinoid Research, Method and Protocols, Human Press inc, USA.
[3] Martono dan Jowana. (2006). Studi Kasus Pemeriksaan Sampel Rambut, Website: http://www.scribd.com/doc/ 60622481/makalah-studi-kasus.
[4] Balikova, M. (2005). Hair Analysis for Drugs of Abuse, Plausibility of Interpretation, Biomed Pap Mede Fac Univ Palacky Olomouc Czech Repub, 149 (2), 199–207. doi.org/10.5507/bp.2005.026.
[5] Indonesian National Narcotics Agency, 2015.
[6] Lestari, S. I (2013). Strategi Badan Narkotika Nasional Kota Samarinda dalam menanggulangi penggunaan narkoba di Kelurahan Sungai Pinang Dalam Kota Samarinda, Ejournal Ilmu Pemerintahan, 1(2), 943-955.
[7] Reggra, H. P. (2009). The Cannabinoid Receptors, Homana Press, USA.
[8] Khajuria, H. & Nayak, B. P. (2014). Detection of Δ9-tetrahydrocannabinol (THC) in hair using GC–MS. Egyptian Journal of Forensic Sciences, 4(1), 17–20. doi.org/10.1016/j.ejfs.2013.10.001.
[9] Starks, M. (1990. Marijuana Chemistry, Genetic, Processing, and Potency, Barkeley, California.
[10] Saito, K. (2011). Analysis of drug of abuse in biological specimens. Journal of Health Science, 57 (6), 472-487.
[11] Rosani, S. (2013). Standar Pelayanan Minimal Terapi Medik ketergantungan narkotika, Psikotropika, dan Bahan adiktif lainnya (Narkoba), BNN, Jakarta.
[12] Swift, W., Wong, A., Li, K. M., Arnold, J. C., & McGregor, I. S. (2013). Analysis of Cannabis Seizures in NSW, Australia: Cannabis Potency and Cannabinoid Profile. PLoS ONE, 8(7), e70052. doi: 10.1371/journal.pone.0070052.
[13] Mechoulam, R. (2009). Cannabinoids, editor Vincenzo di Marzo, John Wiley and Sons, USA.
[14] Haller, D. L., Acosta, M. C., Lewis, D., Miles, D. R., Schiano, T., Shapiro, P. A., … Newville, H. (2010). Hair Analysis Versus Conventional Methods of Drug Testing in Substance Abusers Seeking Organ Transplantation. American Journal of Transplantation, 10(5), 1305–1311. doi: 10.1111/j.1600-6143.2010.03090.x.
[15] Musshoff, F., Junker, H. P., Lachenmeier, D. W., Kroener, L., & Madea, B. (2002). Fully Automated Determination of Cannabinoids in Hair Samples using Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry. Journal of Analytical Toxicology, 26(8), 554–560. doi: 10.1093/jat/26.8.554.
[16] Hegstad, S., Khiabani, H. Z., Kristoffersen, L., Kunoe, N., Lobmaier, P. P., & Christophersen, A. S. (2008). Drug Screening of Hair by Liquid Chromatography-Tandem Mass Spectrometry. Journal of Analytical Toxicology, 32(5), 364–372. doi: 10.1093/jat/32.5.364.
[17] Anggraini. (2015). Tinjauan kriminologi penyalahgunaan narkotika yang dilakukan oleh perempuan di Sungguminasa, Perpustakaan Pusat Universitas Hasanuddin, Indonesia.
[18] Baumgartner, A. (1979). Radioimmunoassay of Hair for Determining Opiate – Abuse Histories, The Journal of Nuclear Medicine, 748–752.
[19] Jones, J., Jones, M., Plate, C., & Lewis, D. (2013). The Detection of THCA Using 2-Dimensional Gas Chromatography-Tandem Mass Spectrometry in Human Fingernail Clippings: Method Validation and Comparison with Head Hair. AJAC, 04(10), 1–8. doi: 10.4236/ajac.2013.410a2001.
[20] Engelhart, D. (2014). Rapid, Robust, and Sensitive Detection of 11-nor-Δ9-Tetrahydrocannabinol-9-Carboxylic Acid in Hair, Application Note, Forensic/Doping Control. Agylent Technologies, USA.
[21] Albertini, T., dan Andrea Caruso. (2015). Determination and Its Main Metabolite Using GC Triple Quadropole Mass Spectrometry. Thermo Scientific Methode, USA.
[22] Rusevska, K., dan Zoran Zdravkovski. (2011). Simple Extraction Method for Detecting Exogenous Substances in Scalp Hair by GCMS. Journal of Hygienic Engineering and Design, Original scientific paper UDC 543.544.3: 543.51, 59–67.
[23] Widayati, D. T. (2008). Analisis Forensik, Departemen Narkoba, BNN, Jakarta.
[24] Abdi, K, Abbas Shafiee, Mohsen Amini, Mahmood Ghazi Khansari, dan Omid Sabzevari. (2004). Detection of Morphine in Opioid Abusers Hair by GC/MS. DARU Journal. 12(2),. 71–75.
[25] Galand, N., D. Ernour, F. Montigny, J. Dollet, dan J. Pothier. (2004). Separation and Identification of Cannabis Components by Different Planar Chromatography Techniques (TLC, AMD, OPLC). Journal of Chromatographyc Science, 42, 130 – 134. doi: 10.1093/chromsci/42.3.130.
[26] Anonimous. (2014). Rapid, Robust, and Sensitive Detection of 11-nor-Δ9-Tetrahydrocannabinol-9-Carboxylic Acid in Hair, Application Note, Forensic/Dopng Control, Agylent Technologies, Inc. USA.
[27] Gouveia, C. A. P. (2011). Simultaneous Quantification of Morphine and Cocaine in Hair Samples by Gas Chromatography-Mass Spectrometry, Master Thesis, University Porto, Portugal.
[28] Harmita. (2004). Petunjuk Pelaksanaan validasi, metode, dan cara perhitungannya, Majalah Ilmu Kefarmasian, Vol. I, No. 3. Hal. 117 – 135.
[29] Nasser, F. (2007). Diagnostic Use of Hair Analysis for the Detection of Misuse of Amfetamines and Cannabinoids, Departement of Forensic Medicine and Science, University of Galgow.
[30] Maunder, et. al. (1974). An improved procedure for the field testing of cannabis, Department of Trade and industry, Laboratory of the Government Chemist, UNODC, London.
[31] Kintz, P. (2003). Hair Analysis, Clark’s Analysis of Drugs and Poisons, 3rd Edition. Volume 1. Pharmaceutical Press. London.
[32] Sharma, P., MM. Srinivas Bharath, dan Pratima Murthy. (2010). Qualitative high performance thin layer chromatography (HPTLC) analysis of cannabinoids in urine samples of Cannabis abusers, Indian Journal Med. Res, 132, 201-208.
[33] Moosmann, B., Roth, N., & Auwärter, V. (2013). Hair analysis for THCA-A, THC and CBN after passive in vivo exposure to marijuana smoke. Drug Testing and Analysis, 6(1-2), 119–125. doi: 10.1002/dta.1474.
[34] Grotenhermen, F. (2002). Cannabis and Cannabinoids, Editor Ethan Russo MD. The Haworth Integrative Heating Press, Birminghamton.
[35] Florian-Ramrez, N. M., Garzn-Mndez, W. F., & Parada-Alfonso, F. (2012). Gas Chromatography in Forensic Chemistry: Cannabinoids Content in Marijuana Leaves (Cannabis sativa L.) from Colombia. Gas Chromatography - Biochemicals, Narcotics and Essential Oils. doi: 10.5772/34503.
[36] Shah, I., Petroczi, A., Uvacsek, M., Ránky, M., & Naughton, D. P. (2014). Hair-based rapid analyses for multiple drugs in forensics and doping: application of dynamic multiple reaction monitoring with LC-MS/MS. Chemistry Central Journal, 8(1). doi: 10.1186/s13065-014-0073-0.
[37] Sell, C. (2003). A Fragrant intoduction to terpenoid Chemistry, The royal society of chemistry press, Cambridege, United of Kingdom.
[38] Yadav VK, A. S. (2014). Microscopical and Chemical Study of Cannabis sativa. J Forensic Res, 05(01). doi: 10.4172/2157-7145.1000210.
[39] Delmifiana, B. (2013). Pengaruh Sonikasi terhadap Struktur dan Morfologi non partikel magnetic yang disintesis dengan Metode Kopresipitasi. Jurnal Fisika Unand 2(3), 186-189.
[40] Anonimous. (2003). Buku Petunjuk Lapangan Pemeriksaan Laboratoris Kriminalistik Barang Bukti Narkotika dan Obat Berbahaya, Pusat Laboratorium Forensik Bareskrim Polri, Jakarta.
[41] Taufik, M. (2013). Deteksi Narkotika Jenis Cannabinol dan Morfin dari sampel urine pengguna narkotika, SNYUbe, Politeknik Negeri Lhokseumawe.
[42] Salomone, A. et al. (2013). Hair analysis as a tool to evaluate the prevalence of synthetic cannabinoids in different populations of drug consumers. Drug Testing and Analysis, 6(1-2), 126–134. doi.org/10.1002/dta.1556.
[43] Musshoff, F., & Madea, B. (2006). Review of Biologic Matrices (Urine, Blood, Hair) as Indicators of Recent or Ongoing Cannabis Use. Therapeutic Drug Monitoring, 28(2), 155–163. doi: 10.1097/01.ftd.0000197091.07807.22.
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    Muhammad Taufik, Harlem Marpaung, Jamaran Kaban, Basuki Wirjosentono. (2016). Analysis of User’s Hair Cannabinoid of Narcotic Type of Marijuana (Cannabis Sativa L.) Using GCMS Technic. American Journal of Biomedical and Life Sciences, 4(1), 1-10. https://doi.org/10.11648/j.ajbls.20160401.11

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    Muhammad Taufik; Harlem Marpaung; Jamaran Kaban; Basuki Wirjosentono. Analysis of User’s Hair Cannabinoid of Narcotic Type of Marijuana (Cannabis Sativa L.) Using GCMS Technic. Am. J. Biomed. Life Sci. 2016, 4(1), 1-10. doi: 10.11648/j.ajbls.20160401.11

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    AMA Style

    Muhammad Taufik, Harlem Marpaung, Jamaran Kaban, Basuki Wirjosentono. Analysis of User’s Hair Cannabinoid of Narcotic Type of Marijuana (Cannabis Sativa L.) Using GCMS Technic. Am J Biomed Life Sci. 2016;4(1):1-10. doi: 10.11648/j.ajbls.20160401.11

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  • @article{10.11648/j.ajbls.20160401.11,
      author = {Muhammad Taufik and Harlem Marpaung and Jamaran Kaban and Basuki Wirjosentono},
      title = {Analysis of User’s Hair Cannabinoid of Narcotic Type of Marijuana (Cannabis Sativa L.) Using GCMS Technic},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {4},
      number = {1},
      pages = {1-10},
      doi = {10.11648/j.ajbls.20160401.11},
      url = {https://doi.org/10.11648/j.ajbls.20160401.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20160401.11},
      abstract = {This paper describes the analysis of cannabinoids in the hair of the user of the narcotic type of marijuana (Cannabis sativa L), using the technique Gas Chromatography Mass Spectroscopy (GCMS) and the validation of the method used. This research was conducted through experiments. Samples were taken from users of narcotic type of marijuana, found in Al-Kamal Sibolangit Rehabilitation Centre, North Sumatra, Indonesia. Marijuana plants were taken from the evidence in Forensic Laboratory of Medan Branch Police. Extraction is done through maceration by sonication at 42 KHz and using methanol – 2 propanol (1:1), kloroform, and petroleum benzene each of 2 ml for 5 minutes. Testing is done with fast blue salt B test and show there is a precipitate Violet. Optimal results obtained at a temperature of 50°C extraction. Identification is done with Thin Layer Chromatography (TLC) in the mobile phase of toluene and n-hexane (1:1) under alkaline conditions of pH 9,5, followed by GC MS techniques for sixteen (16) minutes. The results are contained compounds of cannabidiol, cannabinol, and Δ9THC (Tetrahydrocannabinoid) with concentrations ranging from 0.25 up to 2.82 ng/mg in the hair of the user. Validation of GC MS method for the compounds of cannabidiol, cannabinol, and Δ9THC produces an accuracy value with %recovery is 103,83, 102,67, and 101,17 respectively. Precision test produces a value of Relatif Standard Deviation (RSD) = 1,3058%, 0,8997%, dan 0,8997% respectively. Linearity test generate r value each of the regression is 0,922, 0,955, and 0,921. Limits of Detection (LOD) is obtained 0,000168 ng/mg, 0,000117 ng/mg, and 0,000164 respectively. Limits of Quantification (LOQ) is 0,00056 ng/mg, 0,00039 ng/mg, and 0,00054 ng/mg respectively. This indicates that the modification process of extraction and GC MS techniques used could produce cannabinoid compounds (cannabidiol, cannabinol, and Δ9THC) in hair samples quickly and accurately.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Analysis of User’s Hair Cannabinoid of Narcotic Type of Marijuana (Cannabis Sativa L.) Using GCMS Technic
    AU  - Muhammad Taufik
    AU  - Harlem Marpaung
    AU  - Jamaran Kaban
    AU  - Basuki Wirjosentono
    Y1  - 2016/03/09
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajbls.20160401.11
    DO  - 10.11648/j.ajbls.20160401.11
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 1
    EP  - 10
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20160401.11
    AB  - This paper describes the analysis of cannabinoids in the hair of the user of the narcotic type of marijuana (Cannabis sativa L), using the technique Gas Chromatography Mass Spectroscopy (GCMS) and the validation of the method used. This research was conducted through experiments. Samples were taken from users of narcotic type of marijuana, found in Al-Kamal Sibolangit Rehabilitation Centre, North Sumatra, Indonesia. Marijuana plants were taken from the evidence in Forensic Laboratory of Medan Branch Police. Extraction is done through maceration by sonication at 42 KHz and using methanol – 2 propanol (1:1), kloroform, and petroleum benzene each of 2 ml for 5 minutes. Testing is done with fast blue salt B test and show there is a precipitate Violet. Optimal results obtained at a temperature of 50°C extraction. Identification is done with Thin Layer Chromatography (TLC) in the mobile phase of toluene and n-hexane (1:1) under alkaline conditions of pH 9,5, followed by GC MS techniques for sixteen (16) minutes. The results are contained compounds of cannabidiol, cannabinol, and Δ9THC (Tetrahydrocannabinoid) with concentrations ranging from 0.25 up to 2.82 ng/mg in the hair of the user. Validation of GC MS method for the compounds of cannabidiol, cannabinol, and Δ9THC produces an accuracy value with %recovery is 103,83, 102,67, and 101,17 respectively. Precision test produces a value of Relatif Standard Deviation (RSD) = 1,3058%, 0,8997%, dan 0,8997% respectively. Linearity test generate r value each of the regression is 0,922, 0,955, and 0,921. Limits of Detection (LOD) is obtained 0,000168 ng/mg, 0,000117 ng/mg, and 0,000164 respectively. Limits of Quantification (LOQ) is 0,00056 ng/mg, 0,00039 ng/mg, and 0,00054 ng/mg respectively. This indicates that the modification process of extraction and GC MS techniques used could produce cannabinoid compounds (cannabidiol, cannabinol, and Δ9THC) in hair samples quickly and accurately.
    VL  - 4
    IS  - 1
    ER  - 

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Author Information
  • Medan Region Police Department, North Sumatra, Medan, Indonesia

  • Faculty of Mathematics and Science, University of North Sumatera, Medan, Indonesia

  • Faculty of Mathematics and Science, University of North Sumatera, Medan, Indonesia

  • Faculty of Mathematics and Science, University of North Sumatera, Medan, Indonesia

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