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Physicochemical and Spectroscopic Properties of Biofield Energy Treated Protose
Mahendra Kumar Trivedi,
Alice Branton,
Dahryn Trivedi,
Gopal Nayak,
Khemraj Bairwa,
Snehasis Jana
Issue:
Volume 3, Issue 6, December 2015
Pages:
104-110
Received:
11 October 2015
Accepted:
20 October 2015
Published:
14 November 2015
Abstract: Protose is the enzyme digest of mixed proteins that is recommended for culture media, bulk production of enzymes, antibiotics, toxins, veterinary preparations, etc. This study was proposed to evaluate the effect of biofield energy treatment on the physicochemical and spectroscopic properties of protose. The study was achieved in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Trivedi’s biofield energy treatment. Finally, both the control and treated samples were evaluated using various analytical techniques. The X-ray diffractograms (XRD) of control and treated samples showed the halo patterns peak that suggested the amorphous nature of both the samples of protose. The particle size analysis showed about 12.68% and 90.94 increase in the average particle size (d50) and d99 (particle size below which 99% particles are present) of treated protose with respect to the control. The surface area analysis revealed the 4.96% decrease in the surface area of treated sample as compared to the control sample. The differential scanning calorimetry (DSC) analysis revealed the 22.49% increase in the latent heat of fusion of treated sample as compared to the control. Thermogravimetric analysis (TGA) analysis showed increase in maximum thermal degradation temperature (Tmax) by 5.02% in treated sample as compared to the control. The increase in Tmax might be correlated with increased thermal stability of treated sample as compared to the control. Fourier transform infrared (FT-IR) study showed the alteration in the vibrational frequency of functional groups like N-H, C-H, and S=O of treated protose as compared to the control sample. Based on the overall analytical results, it is concluded that Mr. Trivedi’s biofield energy treatment has a significant impact on the physicochemical and spectral properties of protose. As a result, the treated protose might be more effective as a culture medium than the corresponding control.
Abstract: Protose is the enzyme digest of mixed proteins that is recommended for culture media, bulk production of enzymes, antibiotics, toxins, veterinary preparations, etc. This study was proposed to evaluate the effect of biofield energy treatment on the physicochemical and spectroscopic properties of protose. The study was achieved in two groups i.e. con...
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Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi
Tahirah Hasan,
Abdul Wahid Wahab,
Natsir Djide,
Muhammad Zakir
Issue:
Volume 3, Issue 6, December 2015
Pages:
111-114
Received:
8 October 2015
Accepted:
19 October 2015
Published:
13 November 2015
Abstract: Antioxidant activity test on protein extract of Kerang Kepah (Atactodea striata) by scavenging method of free radical of DPPH (N, N-diphenyl-N-picrylhydrazyl) has been carried out. This study was conducted to determine the antioxidant activity and the amino acid composition of the protein extract of Atactodea striata. The analysis results showed that the protein extract of Atactodea striata has the most complete amino acid composition and the highest antioxidant activity of the protein fraction at a rate of 30-50% ammonium sulfate saturation with IC50 value of 183.75µg/mL. However, when it is compared to the antioxidant activity of ascorbic acid (IC50 values = 2.51µg/mL), the antioxidant activity of the protein extract fraction of Atactodea striata is still much lower.
Abstract: Antioxidant activity test on protein extract of Kerang Kepah (Atactodea striata) by scavenging method of free radical of DPPH (N, N-diphenyl-N-picrylhydrazyl) has been carried out. This study was conducted to determine the antioxidant activity and the amino acid composition of the protein extract of Atactodea striata. The analysis results showed th...
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Production Optimization and Characterization of Amylase Enzyme Isolated from Termofil Bacteria Bacillus sp RSAII-1b from Lejja Hot Spring South Sulawesi
Rugaiyah A. Arfah,
Ahyar Ahmad,
M. Natsir Djide,
Mahdalia Anis,
Muhammad Zakir
Issue:
Volume 3, Issue 6, December 2015
Pages:
115-119
Received:
22 November 2015
Accepted:
7 December 2015
Published:
30 December 2015
Abstract: Thermostable amylase enzyme has a broad commercial value in its use in the processing of starch, sugar production, textile, paper, animal feed, pharmaceuticals and in the manufacture of detergents. This study aims to determine the optimum conditions of amylase production from the termofil bacteria Bacillus sp. RSII-1b isolated from a hot spring Lejja South Sulawesi and characterizing the amylase enzyme. The testing of amylase production was done with various concentration of starch and CaCl2 in the production medium, then fermented to obtain maximum amylase activity, amylase enzyme was produced in optimum condition, and its characteristic was tested using 2% starch substrates in various pH, temperature and determining the compound cofactor which can act as activators or inhibitors of the amylase activity, the enzyme activity was tested using DNS method. Crude extract enzyme has the highest enzyme activity of the protein content determined by the method of Lawry. The results showed that the amylase enzyme from Bacillus sp RSAII-1b isolates can be manufactured to a maximum at 33 hours of fermentation time with conditions: the concentration of substrate (starch) 1.5%, 0.08% CaCl2, 55°C temperature, medium pH 7.0 and aeration speed 200rpm with the activity of 0.1323U/mL, amylase crude extract protein content of 1.86mg/mL, with spesifik activity 0.0711 U/mg protein. Crude extract amylase work optimally at pH 6.0; 55°C -60°C the amylase activity of 0.165U/mL, the specific activity of 0.089U/mg protein. Amylase enzyme is an enzyme that depends on metal because its catalytic activity can be activated by metal ions Ca2+, Mg2+, Cu2+, Ni2+ and Co2+ as activators whereas Zn2+ ions decrease the activity of enzymes as inhibitors. Amylase activity in the crude extract optimum conditions with the addition of 10 mm ions Ca2+ can increase amylase activity up to 32,89%, while the addition of ions Zn2+ can inhibit amylase activity up to 25%.
Abstract: Thermostable amylase enzyme has a broad commercial value in its use in the processing of starch, sugar production, textile, paper, animal feed, pharmaceuticals and in the manufacture of detergents. This study aims to determine the optimum conditions of amylase production from the termofil bacteria Bacillus sp. RSII-1b isolated from a hot spring Lej...
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Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study
Dinushka Wickramasinghe,
Hemantha Peiris,
Lal Gotabhaya Chandrasena,
Vajira Senaratne,
Rasika Perera
Issue:
Volume 3, Issue 6, December 2015
Pages:
120-126
Received:
13 December 2015
Accepted:
23 December 2015
Published:
4 January 2016
Abstract: Glutathione peroxidase and paraoxonase-1 is reported to be a useful marker for monitoring cardiovascular disease. Due to the paucity of information on the association of above markers and severity of coronary artery disease in South Asian patients, case control study was performed with 85 patients (58 males and 27 females) 40-60 years of age confirmed as having coronary artery disease on coronary angiography findings and 85 age and sex matched healthy volunteers as controls. Blood samples were analyzed for serum paraoxonase-1 and erythrocyte Glutathione peroxidase activity in both groups and the severity of coronary artery disease was assessed using coronary angiographic scoring system based on vessel, stenosis and extent score. Patients with coronary artery disease showed significantly low paraoxonase-1 and Glutathione peroxidase activity compared to control subjects. However, according to the best cutoff value determined by receiver operating characteristic analysis for serum paraoxonase-1 (38μg/mL) did not show a significantly high sensitivity, negative predictive value and negative likelihood ratio when compared to erythrocyte Glutathione peroxidase (84.5U/gHb) in predicting the severity of coronary artery disease assessed by three angiographic scores. Glutathione peroxidase appears to be an accurate marker in ruling out major coronary vessel disease and luminal narrowing by atheroma.
Abstract: Glutathione peroxidase and paraoxonase-1 is reported to be a useful marker for monitoring cardiovascular disease. Due to the paucity of information on the association of above markers and severity of coronary artery disease in South Asian patients, case control study was performed with 85 patients (58 males and 27 females) 40-60 years of age confir...
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Antimicrobial Susceptibility Patterns of Enterobacteriaceae Isolated from Stool Samples at a Semi-urban Teaching Hospital
Charles John Elikwu,
Emmanuel Olushola Shobowale,
Victor Ugochukwu Nwadike,
Babatunde Tayo,
Chika Celen Okangba,
Opeoluwa Akinyele Shonekan,
Azubuike Chidiebere Omeonu,
Bibitayo Faluyi,
Pearl Ile,
Adebola Adelodun,
Adebusola Popoola,
Maxwell Mubele
Issue:
Volume 3, Issue 6, December 2015
Pages:
127-130
Received:
16 December 2015
Accepted:
27 December 2015
Published:
11 January 2016
Abstract: Enterobacteriaceae cause a wide range of diseases including urinary tract infections, respiratory tract infections, sepsis, and gastroenteritis. They are the most frequently recovered pathogens from clinical samples and have varying susceptibility patterns. The study set out to determine the susceptibility profile of enterobacteriaceae species at the Babcock University Teaching Hospital. Enterobacteriaceae were identified using the Microbact 12A kit (Oxoid UK) and susceptibility was determined with the modified Kirby-Bauer Method in line with CLSI 2014 guidelines. Escherichia coli the main isolate was 100% susceptible to Piperacillin/Tazobactam, 94% susceptible to Amikacin, 76.5% susceptible to both Ampicillin/Sulbactam and Ceftazidime, 70.6% susceptible to Ceftriaxone and Meropenem, 67% susceptible to Ciprofloxacin, 58% Susceptible to Gentamicin and 23.5% susceptible to Amoxicillin/Clavulanic acid. Antibiotic resistance among Enterobacteriaceae is on the rise in Babcock University Teaching Hospital. Measures should be put in place to prevent more resistance and to prevent spread of resistant strains.
Abstract: Enterobacteriaceae cause a wide range of diseases including urinary tract infections, respiratory tract infections, sepsis, and gastroenteritis. They are the most frequently recovered pathogens from clinical samples and have varying susceptibility patterns. The study set out to determine the susceptibility profile of enterobacteriaceae species at t...
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