Cytotoxic and Phytotoxic Activities of Reinwardtia trigyna (ROXB.)
Saima,
Shafqat Ali Khan,
Muhammad Ibrar,
Barkatullah
Issue:
Volume 3, Issue 2, March 2017
Pages:
32-36
Received:
12 February 2017
Accepted:
20 February 2017
Published:
17 March 2017
Abstract: The present study was carried out to check the cytotoxic and phytotoxic activities of the ethanolic extract of leaf of Reinwardtia trigyna. The cytotoxic potential checked through Artemia salina mortality test which showed low cytotoxic effect (30% and 43.33%) at the doses of 10µg/ml, and 100µg/ml, while moderate cytotoxic effect (53.33%) was observed at 1000µg/ml with highest LD50, value 4496.29µg/ml as compared to the control having normal saline that shows no mortality. The same extracts showed significant dose dependent phytotoxicity 32.25%, 61.29% and 70.96% inhibition of Lemna minor at the doses of 10, 100 and 1000µg/ml as compared to the positive control “Atrazine sulphate” that shows 100% frond inhibition. The FI50 value of plant extract was very low as 58.448 µg/ml. it is concluded from the present work that Reinwardtia trigyna could be used as a natural herbicide and weedicide as it shows a good phytotoxic activity.
Abstract: The present study was carried out to check the cytotoxic and phytotoxic activities of the ethanolic extract of leaf of Reinwardtia trigyna. The cytotoxic potential checked through Artemia salina mortality test which showed low cytotoxic effect (30% and 43.33%) at the doses of 10µg/ml, and 100µg/ml, while moderate cytotoxic effect (53.33%) was obser...
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Structural Properties and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Energy of Consciousness Using LC-MS and NMR Spectroscopy
Mahendra Kumar Trivedi,
Alice Branton,
Dahryn Trivedi,
Gopal Nayak,
Ariadne Esmene Afaganis,
Barbara Marie Bader,
Brian A. Weekes,
Daphne Luisa Dumas,
Denise Marie Fiedler,
Dennille Mellesia Smith,
Desi Pano,
Donna Felice Galla,
Donna Maria Alija,
Elaine Barbara Mullins,
Elaine M. Scorza,
Ellia O'Donnell,
Fabio Massimo Paciucci,
Frances Goodman Warlick,
Haddon Norman Salt,
Inthirani Arul,
Jacqueline Y. Andrews,
James Jay McLeran,
James Stephen Burnett,
Jean Caroline White,
Parthasarathi Panda,
Kalyan Kumar Sethi,
Snehasis Jana
Issue:
Volume 3, Issue 2, March 2017
Pages:
37-46
Received:
6 February 2017
Accepted:
27 February 2017
Published:
20 March 2017
Abstract: The present study was aimed to evaluate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect®- Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The LC-MS analysis of both the control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min with almost similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly altered compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) and PM+2/PM (18O/16O or 26Mg/24Mg) were significantly decreased in the treated sample by 35.97% and 66.77%, respectively compared with the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly reduced compared with the control sample. Thus, The Trivedi Effect® treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Trivedi Effect® Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects.
Abstract: The present study was aimed to evaluate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two par...
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Genotypic Differences among Mung Bean (Vigna radiata L.) Genotypes for Yield and Associated Traits
Fahim Ullah Khan,
Muhammad Khan,
Mirza Hassan,
Rozina Gul
Issue:
Volume 3, Issue 2, March 2017
Pages:
47-50
Received:
15 May 2016
Accepted:
3 March 2017
Published:
21 March 2017
Abstract: Fourteen mung bean genotypes were evaluated at Barani Agricultural Research Station, Kohat during 2014 in randomized complete block design with three replications. Analysis of variance depicted significant differences among genotypes for all the traits studied. Maximum plant height was recorded for genotype VC-6321 (105.7cm), pod length for Mung NM-06 (9.7cm), seeds pod-1 for NM-19-19 (12), biological yield for NM-92 (13111.1 kgha-1), whereas Mung NM11 showed maximum values for pods plant-1 (22.5), 1000-grain weight (55.6 g), grain yield (3401.8 kg ha-1) and harvest index (36.5%). Genotype Mung NM11 showed highest values pods plant-1, 1000-grain weight and grain yield and could be recommended for general cultivation in Kohat region.
Abstract: Fourteen mung bean genotypes were evaluated at Barani Agricultural Research Station, Kohat during 2014 in randomized complete block design with three replications. Analysis of variance depicted significant differences among genotypes for all the traits studied. Maximum plant height was recorded for genotype VC-6321 (105.7cm), pod length for Mung NM...
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