Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (Triticum aestivum L.) Callus
Helmee Hamed Khuder,
Yusra Ismail Hussein AL-Taei
Issue:
Volume 1, Issue 4, November 2015
Pages:
91-94
Received:
1 August 2015
Accepted:
18 August 2015
Published:
24 September 2015
Abstract: The experiment was conducted to study the effect of sodium chloride (NaCl) at the concentrations of 0.0, 0.5, 1.0 and 1.5% on the callus cells. The Iraq wheat variety was grown in vitro for the purpose of knowing the effect of salt stress on some indicators and cellular components of callus by using a randomized complete design, at the laboratories of tissue culture propagation date palm unit in the College of Agriculture / University of Kufa during the period 2014-2015. Fresh and dry weight, the rate of absolute growth, percentage of dry matter of callus, content of the callus cells of proline, total soluble carbohydrates, sodium and potassium ions, effectiveness of the enzymes catalase and peroxidase study shock salt proteins in callus were determined as indicators of the effect of NaCl on the callus culture cells in the above salty medium and the results can be summarized, to control treatment achieved the highest average of (fresh callus weight, dry callus weight, the absolute growth rate of potassium ion), which was (0.0805 g, 6.13 mg, 3.147 mg / day and 1.125%) respectively, while the conc. 1.5% NaCl gave highest average in callus content of (proline, total soluble carbohydrates, sodium ion, the effectiveness of Catalase and Peroxidase enzyme and Salt shock proteins), which (0.134 mg / g, 2.9527 mg / g, 0.1050%, 0.1510 Micromol / protein / min, 0.1127 Micromol / protein / min and the emergence of small molecular weights), respectively.
Abstract: The experiment was conducted to study the effect of sodium chloride (NaCl) at the concentrations of 0.0, 0.5, 1.0 and 1.5% on the callus cells. The Iraq wheat variety was grown in vitro for the purpose of knowing the effect of salt stress on some indicators and cellular components of callus by using a randomized complete design, at the laboratories...
Show More
Estimation of the Interaction Effect Between Salinity and Growth Regulators on Salt Tolerance of Two Bread Wheat Cultivars
Ibrahim I. Al-Mishhadani,
Eman N. Ismail,
Khudhair A. Jaddoa,
Duha M. Majeed,
Omer A. Mohammed
Issue:
Volume 1, Issue 4, November 2015
Pages:
95-101
Received:
25 September 2015
Accepted:
6 October 2015
Published:
23 October 2015
Abstract: The objective of this study is to investigate the effect of the interaction between salinity and plant growth regulators (Kinetin and gibberellin) on the salt tolerance of two bread wheat cultivars (Dijilla) and (Tamooze-2) under salinity conditions. The present study was conducted to assess the effects of Kinetin and gibberellin on plant dry matters (shoots and roots), chlorophyll content and stability in upper leaves, salt tolerance index, and organic contents in plant leaves of the two cultivars grown in salinized soils at two levels (0 and 16 ds/m). Seeds of both cultivars were sown in plastic pots which filled with the salinized soil in glass house during 2014-2015 sessions. Selected cultivar gave the highest dry matters, chlorophyll content and stability, and salt tolerance index under salinity condition as compared with local cultivar. Also the selected cultivar had the highest organic compounds in their leaves, especially under salinity condition. Both plant growth regulators were effective in increasing all the measured characters of both cultivars under non-saline condition but at salinity condition were effective in selected cultivar only. The Kinetin was more effective than the gibberellin in their effect on the salt tolerance of the plants under saline condition. Generally under these conditions the selected cultivar (Dijilla) was more salt tolerance than local cultivar.
Abstract: The objective of this study is to investigate the effect of the interaction between salinity and plant growth regulators (Kinetin and gibberellin) on the salt tolerance of two bread wheat cultivars (Dijilla) and (Tamooze-2) under salinity conditions. The present study was conducted to assess the effects of Kinetin and gibberellin on plant dry matt...
Show More
