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Cytotoxic and Phytotoxic Activities of Reinwardtia trigyna (ROXB.)

Received: 12 February 2017     Accepted: 20 February 2017     Published: 17 March 2017
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Abstract

The present study was carried out to check the cytotoxic and phytotoxic activities of the ethanolic extract of leaf of Reinwardtia trigyna. The cytotoxic potential checked through Artemia salina mortality test which showed low cytotoxic effect (30% and 43.33%) at the doses of 10µg/ml, and 100µg/ml, while moderate cytotoxic effect (53.33%) was observed at 1000µg/ml with highest LD50, value 4496.29µg/ml as compared to the control having normal saline that shows no mortality. The same extracts showed significant dose dependent phytotoxicity 32.25%, 61.29% and 70.96% inhibition of Lemna minor at the doses of 10, 100 and 1000µg/ml as compared to the positive control “Atrazine sulphate” that shows 100% frond inhibition. The FI50 value of plant extract was very low as 58.448 µg/ml. it is concluded from the present work that Reinwardtia trigyna could be used as a natural herbicide and weedicide as it shows a good phytotoxic activity.

Published in International Journal of Applied Agricultural Sciences (Volume 3, Issue 2)
DOI 10.11648/j.ijaas.20170302.11
Page(s) 32-36
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Reinwardtia trigyna, Ethanolic Extract, Leaf, Cytotoxic Activity, Artemia salina, Phytotoxic Activity, Lemna minor

References
[1] Atta-ur-Rhman, M. I. Choudhary and W. J. Thomsen. 2001. Bioassay Technique for Drug Development Harwood Academic Publishers.
[2] McLaughlin, J. L and J. E. Anderson. 1988. Brine shrimp and crown gall tumors: simple bioassay for the discovery of plant antitumour agents. In Proceedings, NIH Workshop, Bioassay for the discovery of antitumor and antiviral agents from natural sources, Bethesda, pp. 22.
[3] Bijalwan, A. (2013). Vegetation Status of Agroforestry Systems in Tehri District of Garhwal Himalaya, India. As. J. Sci and Tech, 4 (12): 011-014.
[4] Khattak, S., H. U. Shah, A. Ahmad and M. Ahmad, M. 2005. Biological effects of indigenous medicinal plants Curcuma longa and Alpinia galanga. Fitoterapia, 76 (2): 254-257.
[5] Krishnaraju, A. V., T. V. Rao, D. Sundararaju, M. Vanisree, H. S, Tsay and G. V. Subbaraju. 2006. Biological screening of medicinal plants collected from Eastern Ghats of India using Artemia salina (brine shrimp test). Int. J. Appl. Sci. Eng., 4 (2): 115-125.
[6] Dastagir, G and F. Hussain. 2013. Phytotoxic and insecticidal activity of plants of family Zygophyllaceae and Euphorbiaceae. Sarhad J. Agric., 29 (1): 83-91.
[7] Ahmad, B., I. Khan, S. Bashir, S. Azam and F. Hussain. 2011. Screening of Ziziphus jujuba for antibacterial, phytotoxic and haemagglutination activities. Afr. J. Biotech., 10 (13): 2514-2519.
[8] Duke, S. O., F. E. Dayan, J. G. Romagni and A. M. Rimando. 2000. Natural Products as Sources of Herbicides: Current Status and Future Trends. Weed Research-Oxford., 40 (1): 99-112.
[9] Meyer, B. N., N. R. Ferrigni, J. E. PutnamJacobsen, D. J. Nichols and J. L. McLaughlin. 1982. Brine shrimp: a convenient general bioassay for active plant constituents. Planta medica., 45 (05): 31-34.
[10] Anwar, M. M., M. A. Kalpana, B. Bhadra, S. Rahman, S. Sarker, M. H. Chowdhury and M. Rahmatullah. 2010. Antihyperglycemic activity and brine shrimp lethality studies on methanol extract of Cajanus cajan (L.) Millsp. leaves and roots. Adv. Nat and Appl. Sci., 4: 311-316.
[11] Atta-ur-Rehman, M. I. Choudhary and Thomsen. 1991. Manual of bioassay techniques for natural product research. Harward Acad. Press, Amsterdam. pp. 82-84.
[12] Malla, B., D. P. Gauchan and R. B. Chhetri. 2014. Medico-ethnobotanical investigations in Parbat district of Western Nepal. J. Med. Pl. Res., 8 (2): 95-108.
[13] Dzhambazov, B., S. Daskalova, A. Monteva and N. Popov. 2002. In Vitro Screening for Antitumour Activity of Clinopodium vulgare L. (Lamiaceae) Extracts. Bio. Pharmaceut. Bullet, 25(4): 499-504.
[14] Einhellig, F. A. 1996. Interactions Involving Allelopathy in Cropping Systems. Agronomy Journal., 88 (6): 886-893.
[15] Newman, D. J and G. M. Gragg. 2007. Natural Products as Sources of New Drugs over the Last 25 Years. J. Nat. Pr., 70 (3): 461-477.
[16] Michael, A. S., C. G. Thompson and M. Abramovitz. 1956. Artemia salina as a test organism for Bioassay. Science., 123 (3194): 464-464.
[17] Carballo, L. J., L. Z. Hernandez-inda, P. Perzer and M. D. Gravalos. 2002. A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products. Bio. Med. Central., 2: 1-10.
[18] Ali, I., Naz, R., Khan, W. N., Gul, R., & Choudhary, M. I. 2009. Biological screening of different root extracts of Euphorbia wallichii. Pak. J. Bot., 41 (4): 1737-1741.
[19] Ali, N., P. K. Vishwakarma, I. khan and M. Sohaib. 2014. In Vitro Antibacterial, Antifungal and Phytotoxic Activities of Ficus Carica Methanolic Leaves Extracts, In. J. Curr. Biotechnol., 2 (2): 11-15.
[20] Parvez, M., B. Ahmad, F. Hussain, J. Ali, S. Muhammad, S. Abbas, S. Hassan, I. Ahmad. 2014. Phytotoxicity Study of Euphorbia granulata Forssk against Lemna minor and Radish Seeds. Life Sci. J., 11 (6): 30-34.
[21] Patil, S. B and C. S. Magdum. 2011. Determination of LC50 values of extracts of Euphorbia hirta linn. and Euphorbia neriifolia linn. using brine shrimp lethality assay. As. J. Res. Pharma. Sci., 1 (2): 42-43.
[22] Perveen, A and M. Qaiser. 2008. Pollen flora of Pakistan-Lix. Linaceae. Pak J Bot., 40: 1819-1822.
[23] Rawat, A., P. S. Rana, D. Molpa and P. Saklani. 2015. Phytochemical composition and antioxidant activity of Reinwardtia indica from selected location of uttrakhand. Int. J. Prog. Eng. Manag, Sci and Hum. 1 (3): 22-29.
[24] Richardson, M. A. 2001. Biopharmacologic and herbal therapies for cancer: research update from NCCAM. The J. Nut., 131 (11): 3037-3040.
[25] Sarkar, B., S. Raihan, N. Sultana, R. Rahman, M. E. Islam, S. Ahmed and S. Akter. 2012. Cytotoxic, antibacterial and free radical scavenging activity studies of the solvent extracts of aerial stems of Equisetum debile Roxb. Int. J. Chem Sci., 10 (1): 19-26.
[26] Uddin, G. H. W., B. S. H. Siddiqui, M. Alam, A. Sadat, A. Ahmad and A. Uddin. 2011. Chemical constituents and phytotoxicity of solvent extracted fractions of stem bark of Grewia optiva Drummond ex burret. Middle-East. J. Sci. Res., 8: 85-91.
[27] Yu, G. U. 2012. Antibacterial and antifungal activity of Andrographis echiodes. J. Anhui Agri. Sci., 12, 048.
[28] Yu, G., M. Xiaoping, W. Zhiyang, W. Ruen, Z. Qin., W. Lijuan and C. Dong. 2012. Acute toxicity of Reinwardtia trigyna water extracts to mice liver and kidney functions and blood cell count. Medicinal Plant., 3 (8): 60-63.
[29] Pisutthanan, S., P. Plianbangchang, N. Pisutthanan, S. Ruanruay and O. Muanrit. 2004. Brine shrimp lethality activity of Thai medicinal plants in the family Meliaceae. Naresuan University Journal, 12 (2): 13-18.
Cite This Article
  • APA Style

    Saima, Shafqat Ali Khan, Muhammad Ibrar, Barkatullah. (2017). Cytotoxic and Phytotoxic Activities of Reinwardtia trigyna (ROXB.). International Journal of Applied Agricultural Sciences, 3(2), 32-36. https://doi.org/10.11648/j.ijaas.20170302.11

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    ACS Style

    Saima; Shafqat Ali Khan; Muhammad Ibrar; Barkatullah. Cytotoxic and Phytotoxic Activities of Reinwardtia trigyna (ROXB.). Int. J. Appl. Agric. Sci. 2017, 3(2), 32-36. doi: 10.11648/j.ijaas.20170302.11

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    AMA Style

    Saima, Shafqat Ali Khan, Muhammad Ibrar, Barkatullah. Cytotoxic and Phytotoxic Activities of Reinwardtia trigyna (ROXB.). Int J Appl Agric Sci. 2017;3(2):32-36. doi: 10.11648/j.ijaas.20170302.11

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  • @article{10.11648/j.ijaas.20170302.11,
      author = {Saima and Shafqat Ali Khan and Muhammad Ibrar and Barkatullah},
      title = {Cytotoxic and Phytotoxic Activities of Reinwardtia trigyna (ROXB.)},
      journal = {International Journal of Applied Agricultural Sciences},
      volume = {3},
      number = {2},
      pages = {32-36},
      doi = {10.11648/j.ijaas.20170302.11},
      url = {https://doi.org/10.11648/j.ijaas.20170302.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20170302.11},
      abstract = {The present study was carried out to check the cytotoxic and phytotoxic activities of the ethanolic extract of leaf of Reinwardtia trigyna. The cytotoxic potential checked through Artemia salina mortality test which showed low cytotoxic effect (30% and 43.33%) at the doses of 10µg/ml, and 100µg/ml, while moderate cytotoxic effect (53.33%) was observed at 1000µg/ml with highest LD50, value 4496.29µg/ml as compared to the control having normal saline that shows no mortality. The same extracts showed significant dose dependent phytotoxicity 32.25%, 61.29% and 70.96% inhibition of Lemna minor at the doses of 10, 100 and 1000µg/ml as compared to the positive control “Atrazine sulphate” that shows 100% frond inhibition. The FI50 value of plant extract was very low as 58.448 µg/ml. it is concluded from the present work that Reinwardtia trigyna could be used as a natural herbicide and weedicide as it shows a good phytotoxic activity.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Cytotoxic and Phytotoxic Activities of Reinwardtia trigyna (ROXB.)
    AU  - Saima
    AU  - Shafqat Ali Khan
    AU  - Muhammad Ibrar
    AU  - Barkatullah
    Y1  - 2017/03/17
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ijaas.20170302.11
    DO  - 10.11648/j.ijaas.20170302.11
    T2  - International Journal of Applied Agricultural Sciences
    JF  - International Journal of Applied Agricultural Sciences
    JO  - International Journal of Applied Agricultural Sciences
    SP  - 32
    EP  - 36
    PB  - Science Publishing Group
    SN  - 2469-7885
    UR  - https://doi.org/10.11648/j.ijaas.20170302.11
    AB  - The present study was carried out to check the cytotoxic and phytotoxic activities of the ethanolic extract of leaf of Reinwardtia trigyna. The cytotoxic potential checked through Artemia salina mortality test which showed low cytotoxic effect (30% and 43.33%) at the doses of 10µg/ml, and 100µg/ml, while moderate cytotoxic effect (53.33%) was observed at 1000µg/ml with highest LD50, value 4496.29µg/ml as compared to the control having normal saline that shows no mortality. The same extracts showed significant dose dependent phytotoxicity 32.25%, 61.29% and 70.96% inhibition of Lemna minor at the doses of 10, 100 and 1000µg/ml as compared to the positive control “Atrazine sulphate” that shows 100% frond inhibition. The FI50 value of plant extract was very low as 58.448 µg/ml. it is concluded from the present work that Reinwardtia trigyna could be used as a natural herbicide and weedicide as it shows a good phytotoxic activity.
    VL  - 3
    IS  - 2
    ER  - 

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Author Information
  • Department of Botany University of Peshawar, KP, Pakistan

  • Department of Botany University of Peshawar, KP, Pakistan

  • Department of Botany University of Peshawar, KP, Pakistan

  • Department of Botany Islamia College University, Peshawar, KP, Pakistan

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