Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (<i>Triticum aestivum</i> L.) Callus

Helmee Hamed Khuder; Yusra Ismail Hussein AL-Taei

doi:doi:10.11648/j.ijaas.20150104.11

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Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (Triticum aestivum L.) Callus

Helmee Hamed Khuder, Yusra Ismail Hussein AL-Taei

Published in International Journal of Applied Agricultural Sciences (Volume 1, Issue 4)

Received: 1 August 2015 Accepted: 18 August 2015 Published: 24 September 2015

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Abstract

The experiment was conducted to study the effect of sodium chloride (NaCl) at the concentrations of 0.0, 0.5, 1.0 and 1.5% on the callus cells. The Iraq wheat variety was grown in vitro for the purpose of knowing the effect of salt stress on some indicators and cellular components of callus by using a randomized complete design, at the laboratories of tissue culture propagation date palm unit in the College of Agriculture / University of Kufa during the period 2014-2015. Fresh and dry weight, the rate of absolute growth, percentage of dry matter of callus, content of the callus cells of proline, total soluble carbohydrates, sodium and potassium ions, effectiveness of the enzymes catalase and peroxidase study shock salt proteins in callus were determined as indicators of the effect of NaCl on the callus culture cells in the above salty medium and the results can be summarized, to control treatment achieved the highest average of (fresh callus weight, dry callus weight, the absolute growth rate of potassium ion), which was (0.0805 g, 6.13 mg, 3.147 mg / day and 1.125%) respectively, while the conc. 1.5% NaCl gave highest average in callus content of (proline, total soluble carbohydrates, sodium ion, the effectiveness of Catalase and Peroxidase enzyme and Salt shock proteins), which (0.134 mg / g, 2.9527 mg / g, 0.1050%, 0.1510 Micromol / protein / min, 0.1127 Micromol / protein / min and the emergence of small molecular weights), respectively.

Published in	International Journal of Applied Agricultural Sciences (Volume 1, Issue 4)
DOI	10.11648/j.ijaas.20150104.11
Page(s)	91-94
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Salt Shock Proteins, Antioxidant Enzymes, Salt Stress, Triticum aestivum L.

References

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APA Style

Helmee Hamed Khuder, Yusra Ismail Hussein AL-Taei. (2015). Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (Triticum aestivum L.) Callus. International Journal of Applied Agricultural Sciences, 1(4), 91-94. https://doi.org/10.11648/j.ijaas.20150104.11

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ACS Style

Helmee Hamed Khuder; Yusra Ismail Hussein AL-Taei. Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (Triticum aestivum L.) Callus. Int. J. Appl. Agric. Sci. 2015, 1(4), 91-94. doi: 10.11648/j.ijaas.20150104.11

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AMA Style

Helmee Hamed Khuder, Yusra Ismail Hussein AL-Taei. Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (Triticum aestivum L.) Callus. Int J Appl Agric Sci. 2015;1(4):91-94. doi: 10.11648/j.ijaas.20150104.11

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@article{10.11648/j.ijaas.20150104.11,
  author = {Helmee Hamed Khuder and Yusra Ismail Hussein AL-Taei},
  title = {Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (Triticum aestivum L.) Callus},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {1},
  number = {4},
  pages = {91-94},
  doi = {10.11648/j.ijaas.20150104.11},
  url = {https://doi.org/10.11648/j.ijaas.20150104.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20150104.11},
  abstract = {The experiment was conducted to study the effect of sodium chloride (NaCl) at the concentrations of 0.0, 0.5, 1.0 and 1.5% on the callus cells. The Iraq wheat variety was grown in vitro for the purpose of knowing the effect of salt stress on some indicators and cellular components of callus by using a randomized complete design, at the laboratories of tissue culture propagation date palm unit in the College of Agriculture / University of Kufa during the period 2014-2015. Fresh and dry weight, the rate of absolute growth, percentage of dry matter of callus, content of the callus cells of proline, total soluble carbohydrates, sodium and potassium ions, effectiveness of the enzymes catalase and peroxidase study shock salt proteins in callus were determined as indicators of the effect of NaCl on the callus culture cells in the above salty medium and the results can be summarized, to control treatment achieved the highest average of (fresh callus weight, dry callus weight, the absolute growth rate of potassium ion), which was (0.0805 g, 6.13 mg, 3.147 mg / day and 1.125%) respectively, while the conc. 1.5% NaCl gave highest average in callus content of (proline, total soluble carbohydrates, sodium ion, the effectiveness of Catalase and Peroxidase enzyme and Salt shock proteins), which (0.134 mg / g, 2.9527 mg / g, 0.1050%, 0.1510 Micromol / protein / min, 0.1127 Micromol / protein / min and the emergence of small molecular weights), respectively.},
 year = {2015}
}

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TY  - JOUR
T1  - Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (Triticum aestivum L.) Callus
AU  - Helmee Hamed Khuder
AU  - Yusra Ismail Hussein AL-Taei
Y1  - 2015/09/24
PY  - 2015
N1  - https://doi.org/10.11648/j.ijaas.20150104.11
DO  - 10.11648/j.ijaas.20150104.11
T2  - International Journal of Applied Agricultural Sciences
JF  - International Journal of Applied Agricultural Sciences
JO  - International Journal of Applied Agricultural Sciences
SP  - 91
EP  - 94
PB  - Science Publishing Group
SN  - 2469-7885
UR  - https://doi.org/10.11648/j.ijaas.20150104.11
AB  - The experiment was conducted to study the effect of sodium chloride (NaCl) at the concentrations of 0.0, 0.5, 1.0 and 1.5% on the callus cells. The Iraq wheat variety was grown in vitro for the purpose of knowing the effect of salt stress on some indicators and cellular components of callus by using a randomized complete design, at the laboratories of tissue culture propagation date palm unit in the College of Agriculture / University of Kufa during the period 2014-2015. Fresh and dry weight, the rate of absolute growth, percentage of dry matter of callus, content of the callus cells of proline, total soluble carbohydrates, sodium and potassium ions, effectiveness of the enzymes catalase and peroxidase study shock salt proteins in callus were determined as indicators of the effect of NaCl on the callus culture cells in the above salty medium and the results can be summarized, to control treatment achieved the highest average of (fresh callus weight, dry callus weight, the absolute growth rate of potassium ion), which was (0.0805 g, 6.13 mg, 3.147 mg / day and 1.125%) respectively, while the conc. 1.5% NaCl gave highest average in callus content of (proline, total soluble carbohydrates, sodium ion, the effectiveness of Catalase and Peroxidase enzyme and Salt shock proteins), which (0.134 mg / g, 2.9527 mg / g, 0.1050%, 0.1510 Micromol / protein / min, 0.1127 Micromol / protein / min and the emergence of small molecular weights), respectively.
VL  - 1
IS  - 4
ER  -

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Author Information

Helmee Hamed Khuder

Department of Field Crops, College of Agriculture, AL-Qasim Green University, Babylon, Iraq
Yusra Ismail Hussein AL-Taei

Department of Field Crops, College of Agriculture, AL-Qasim Green University, Babylon, Iraq

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Helmee Hamed Khuder, Yusra Ismail Hussein AL-Taei. (2015). Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (Triticum aestivum L.) Callus. International Journal of Applied Agricultural Sciences, 1(4), 91-94. https://doi.org/10.11648/j.ijaas.20150104.11

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ACS Style

Helmee Hamed Khuder; Yusra Ismail Hussein AL-Taei. Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (Triticum aestivum L.) Callus. Int. J. Appl. Agric. Sci. 2015, 1(4), 91-94. doi: 10.11648/j.ijaas.20150104.11

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AMA Style

Helmee Hamed Khuder, Yusra Ismail Hussein AL-Taei. Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (Triticum aestivum L.) Callus. Int J Appl Agric Sci. 2015;1(4):91-94. doi: 10.11648/j.ijaas.20150104.11

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@article{10.11648/j.ijaas.20150104.11,
  author = {Helmee Hamed Khuder and Yusra Ismail Hussein AL-Taei},
  title = {Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (Triticum aestivum L.) Callus},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {1},
  number = {4},
  pages = {91-94},
  doi = {10.11648/j.ijaas.20150104.11},
  url = {https://doi.org/10.11648/j.ijaas.20150104.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20150104.11},
  abstract = {The experiment was conducted to study the effect of sodium chloride (NaCl) at the concentrations of 0.0, 0.5, 1.0 and 1.5% on the callus cells. The Iraq wheat variety was grown in vitro for the purpose of knowing the effect of salt stress on some indicators and cellular components of callus by using a randomized complete design, at the laboratories of tissue culture propagation date palm unit in the College of Agriculture / University of Kufa during the period 2014-2015. Fresh and dry weight, the rate of absolute growth, percentage of dry matter of callus, content of the callus cells of proline, total soluble carbohydrates, sodium and potassium ions, effectiveness of the enzymes catalase and peroxidase study shock salt proteins in callus were determined as indicators of the effect of NaCl on the callus culture cells in the above salty medium and the results can be summarized, to control treatment achieved the highest average of (fresh callus weight, dry callus weight, the absolute growth rate of potassium ion), which was (0.0805 g, 6.13 mg, 3.147 mg / day and 1.125%) respectively, while the conc. 1.5% NaCl gave highest average in callus content of (proline, total soluble carbohydrates, sodium ion, the effectiveness of Catalase and Peroxidase enzyme and Salt shock proteins), which (0.134 mg / g, 2.9527 mg / g, 0.1050%, 0.1510 Micromol / protein / min, 0.1127 Micromol / protein / min and the emergence of small molecular weights), respectively.},
 year = {2015}
}

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TY  - JOUR
T1  - Effect of Salt Stress on Some Growth Indicators and Cellular Components of Wheat (Triticum aestivum L.) Callus
AU  - Helmee Hamed Khuder
AU  - Yusra Ismail Hussein AL-Taei
Y1  - 2015/09/24
PY  - 2015
N1  - https://doi.org/10.11648/j.ijaas.20150104.11
DO  - 10.11648/j.ijaas.20150104.11
T2  - International Journal of Applied Agricultural Sciences
JF  - International Journal of Applied Agricultural Sciences
JO  - International Journal of Applied Agricultural Sciences
SP  - 91
EP  - 94
PB  - Science Publishing Group
SN  - 2469-7885
UR  - https://doi.org/10.11648/j.ijaas.20150104.11
AB  - The experiment was conducted to study the effect of sodium chloride (NaCl) at the concentrations of 0.0, 0.5, 1.0 and 1.5% on the callus cells. The Iraq wheat variety was grown in vitro for the purpose of knowing the effect of salt stress on some indicators and cellular components of callus by using a randomized complete design, at the laboratories of tissue culture propagation date palm unit in the College of Agriculture / University of Kufa during the period 2014-2015. Fresh and dry weight, the rate of absolute growth, percentage of dry matter of callus, content of the callus cells of proline, total soluble carbohydrates, sodium and potassium ions, effectiveness of the enzymes catalase and peroxidase study shock salt proteins in callus were determined as indicators of the effect of NaCl on the callus culture cells in the above salty medium and the results can be summarized, to control treatment achieved the highest average of (fresh callus weight, dry callus weight, the absolute growth rate of potassium ion), which was (0.0805 g, 6.13 mg, 3.147 mg / day and 1.125%) respectively, while the conc. 1.5% NaCl gave highest average in callus content of (proline, total soluble carbohydrates, sodium ion, the effectiveness of Catalase and Peroxidase enzyme and Salt shock proteins), which (0.134 mg / g, 2.9527 mg / g, 0.1050%, 0.1510 Micromol / protein / min, 0.1127 Micromol / protein / min and the emergence of small molecular weights), respectively.
VL  - 1
IS  - 4
ER  -

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