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The Analysis Mutation of the CARD15 Gene Variants in Chronic Periodontitis
Asmawati Amin,
Bahruddin Thalib,
Mochammad Hatta
Issue:
Volume 2, Issue 6, November 2014
Pages:
117-122
Received:
1 October 2014
Accepted:
17 October 2014
Published:
30 October 2014
Abstract: Purpose of this study was to determine the variant of CARD 15 gene mutations with periodontitis chronic .Identified by using polymerase chain reaction (PCR) and folowed by using restriction fragment length polymorphism analysis (RFLP). CARD 15 gene mutation with chronic periodontitis were found heterozygote mutation and homozygote mutation variants base on statistical analysis there were significant with control group ( p= 0,005) and also was founded genetics variation that changed the composition of C → T nucleotide at codon 802 in exon 4 amino acid, from alanine to valine.
Abstract: Purpose of this study was to determine the variant of CARD 15 gene mutations with periodontitis chronic .Identified by using polymerase chain reaction (PCR) and folowed by using restriction fragment length polymorphism analysis (RFLP). CARD 15 gene mutation with chronic periodontitis were found heterozygote mutation and homozygote mutation variants...
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Comparative Effects of Exercise on Selected Cardiorespiratory Parameters among Young Adults from Different Ethnic Groups Resident in Nigeria
Alese Ojo Oluwole,
Adeosun Olukayode Isaac,
Johnson Esther Olubusola,
Olukiran Sesan Olaoluwa,
Alese Margaret Olutayo
Issue:
Volume 2, Issue 6, November 2014
Pages:
123-127
Received:
10 October 2014
Accepted:
1 November 2014
Published:
10 November 2014
Abstract: This study investigated the differences or similarities in the baseline and post exercise cardiorespiratory parameters between young male adults of Igbo and Yoruba. A total of two hundred apparently healthy subjects (100 Yorubas and 100 Igbos) were involved in the study. Subjects were undergraduates who were within the age of 18-30 years. The subjects were asked to sit down for 3 mins and resting cardiorespiratory parameters (pulse rate, blood pressure, forced vital capacity, forced expiratory volume in 1 sec and peak expiratory flow rate) were obtained with the sphygmomanometer and spirometer accordingly. They were then subjected to 3mins of exercise on a step bench while the metronome was set at 88 and 96 beats per minutes. The results showed a significant decrease in pre exercise pulse rate, systolic blood pressure, diastolic blood pressure of Igbo when compared with Yoruba, but there was no significant differences in post exercise systolic and diastolic blood pressure of Igbo when compared with Yoruba. A significant increase in pre and post exercise forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), and VO2 max was observed in Igbo when compared with Yoruba. The study concluded that there were differences in some cardiorespiratory parameters among male participants of both ethnic groups (Igbo and Yoruba) before and after exercise. The reported data may be useful in interpreting more accurately the significance of increase or decrease in cardiorespiratory response before and after exercise in a subject of a specific ethnic group.
Abstract: This study investigated the differences or similarities in the baseline and post exercise cardiorespiratory parameters between young male adults of Igbo and Yoruba. A total of two hundred apparently healthy subjects (100 Yorubas and 100 Igbos) were involved in the study. Subjects were undergraduates who were within the age of 18-30 years. The subje...
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Constitutional Pericentric Inversion of Chromosome 9 and Chronic Myeloid Leukemia
Salil Vaniawala,
Gadhia Pankaj
Issue:
Volume 2, Issue 6, November 2014
Pages:
128-131
Received:
10 October 2014
Accepted:
6 November 2014
Published:
20 November 2014
Abstract: In the present study, we screened the bone marrow chromosome database entries between March, 2004 and December, 2013 to identify cases with inv(9) along with t(9;22) variations. Our study recorded 2300 cases of confirmed CML (Ph positive), of which only 12 (0.52%) cases had inv(9) and t(9;22). The association between inv(9) and t(9;22) is not fully explored. Therefore more number of cases is required to shed light on its important role, if any, especially in bone-marrow transplantation and drug resistance.
Abstract: In the present study, we screened the bone marrow chromosome database entries between March, 2004 and December, 2013 to identify cases with inv(9) along with t(9;22) variations. Our study recorded 2300 cases of confirmed CML (Ph positive), of which only 12 (0.52%) cases had inv(9) and t(9;22). The association between inv(9) and t(9;22) is not fully...
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Caffeine Modulates Biliary Secretions in Indigenous Nigerian Dogs
Kolawole Victor Olorunshola,
Cheh Augustine Awasum,
Nenadi Claire Hedima
Issue:
Volume 2, Issue 6, November 2014
Pages:
132-136
Received:
29 September 2014
Accepted:
7 November 2014
Published:
17 November 2014
Abstract: This study was aimed at evaluating the effect of caffeine on biliary secretions in indigenous Nigerian dogs. A total of 6 dogs weighing 12 – 15 kg divided into 2 groups were used. The control group was fed their normal diet and water ad libitum and the treated group received 16 mg/kg of white crystalline caffeine dissolved in 10 mls of water and administered orally 8 hours prior to each surgery. Under sodium thiopentone and ketamine anaesthesia, common bile duct cannulation was done by the modified method of Rath and Hutchison. Bile was collected immediately post cannulation over a period of 48 to 72 hours. The bile samples from all dogs were analysed for bile volume, pH and electrolyte concentrations. The results showed significant increase in the bile volume in the caffeine treated group: 3.41 ± 0.85 ml compared to the control group: 1.24 ± 0.17 ml (p<0.05). The bile pH in the caffeine treated group: 7.40 ± 0.24 was significantly higher than the control group: 6.68 ± 0.18 (p< 0.05). The potassium concentration of 6.08 ± 0.49mmol/L in control group was significantly higher than the potassium concentration of 4.81 ± 0.21mmol/L in the treated group (p< 0.05). However, there was no significant change in the concentration of bicarbonate, chloride and sodium ions in the caffeine treated animals. We conclude that orally administered caffeine significantly increased bile volume and bile PH and significantly decreased bile potassium concentration in indigenous Nigerian dogs and these findings may have implication for digestion and absorption of fat soluble vitamins and a measure of liver functions.
Abstract: This study was aimed at evaluating the effect of caffeine on biliary secretions in indigenous Nigerian dogs. A total of 6 dogs weighing 12 – 15 kg divided into 2 groups were used. The control group was fed their normal diet and water ad libitum and the treated group received 16 mg/kg of white crystalline caffeine dissolved in 10 mls of water and ad...
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Efficacy of Topical Zinc Sulphate on Wound Healing of Experimentally Induced Skin Ulcers by Nicorandil and Induction Effect on Transforming Growth Factor-β
Shireen Sameen,
Tagreed Altaei
Issue:
Volume 2, Issue 6, November 2014
Pages:
137-150
Received:
5 November 2014
Accepted:
12 November 2014
Published:
17 November 2014
Abstract: Induced ulcer wound repair needs re-epithelialisation to replace necrotised and/or damaged tissue and to re-establish its integrity. Transforming growth factor-β (TGF-β) affects all cell types that are involved in all stages of wound healing. The assessment of induced ulcer wound healing activity was carried out through three models: First, excision model, second, induced ulcer wound using 50 mg Aspirin, and third by using 10 mg Nicorandil wound model. The animals were divided into eight groups; and were treated with different concentrations of Zinc sulphate, compared to their controls. The efficacy of Zinc sulphate on healing process of lesion induced was assessed by; body weight change, macroscopical appearance of the induced ulcers (ulcer area and wound contraction), microscopically appearance and histopathology; as well as qualitative assessment Moreover, the effect of ulcer induction on the serum levels of TGF-β according to the procedure of the kit was assessed in all groups. Different concentrations of topical Zinc sulphate have not significantly enhanced the healing of excision wounds. Thirty milligram Zinc sulphate has not significantly enhanced the healing of lesion ulcer induced by 50 mg Aspirin or 10 mg Nicorandil. Nevertheless, the 50 mg Aspirin and 10 mg Nicorandil caused delay in healing. In all groups, a significant reduction in ulcer area was observed compared to that of the baseline. Besides, a significant elevation in wound contraction was noticed compared to that of the baseline. Qualitative assessment of the healing process of the induced ulcer confirms that Aspirin or Nicorandil delay healing. Assessment of serum TGF-β level in serum showed no statistical significant elevation in those groups treated with different concentrations of Zinc sulphate compared to their control, while TGF-β level showed non-significant reduction in the group of induced ulcer by Aspirin or Nicorandil and treatment with 30 mg Zinc sulphate, compared to their controls. In conclusion, Nicorandil cause ulceration in the same manner of Aspirin, which delay the healing process, and TGF-β appears to play a more profound role in the healing process perforations than in the healing of induced ulcers in skin.
Abstract: Induced ulcer wound repair needs re-epithelialisation to replace necrotised and/or damaged tissue and to re-establish its integrity. Transforming growth factor-β (TGF-β) affects all cell types that are involved in all stages of wound healing. The assessment of induced ulcer wound healing activity was carried out through three models: First, excisi...
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The Effect of Arm Swing Exercise on Gait and Balance in Stroke Patients
June-Seok Ma,
Hyun-Joo Kim
Issue:
Volume 2, Issue 6, November 2014
Pages:
151-155
Received:
19 October 2014
Accepted:
3 November 2014
Published:
21 November 2014
Abstract: The aim of this study is investigate the effect of arm swing exercise on balance and gait in stroke patients. Group A (n=8): 0.5kg strap was applied during arm swing exercise in experimental group for 30 minutes a day, 3 times a week for 4 weeks. Group B (n=8): Subjects in control group performed neurodevelopmental treatment for 30 minutes a day, 3 times a week for 4 weeks. Subjects were measured for balance and gait on Berg Balance scale, 10meter walking test and Six-minute walk test. There were significant changed of 10Meter Walking Test and 6-Minute Walk Test after experimental group performed the reinforced arm swing exercise of upper extremities and control group was provided with neurodevelopment treatment(p <0.05). However, there was no statistically significant found difference change of Berg Balance Scale in experimental group (p >0.05). The application reinforced arm swing exercise can be said to be effective intervention for the gait training in hemiplegic stroke patient.
Abstract: The aim of this study is investigate the effect of arm swing exercise on balance and gait in stroke patients. Group A (n=8): 0.5kg strap was applied during arm swing exercise in experimental group for 30 minutes a day, 3 times a week for 4 weeks. Group B (n=8): Subjects in control group performed neurodevelopmental treatment for 30 minutes a day, 3...
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E-Prostanoid Receptor Distribution in Airway Smooth Muscle Cells of a Rat Model of Asthma
Yanfeng Ying,
Ye Hu,
Haohao Chen,
Pingguang Tu
Issue:
Volume 2, Issue 6, November 2014
Pages:
156-160
Received:
3 November 2014
Accepted:
13 November 2014
Published:
23 November 2014
Abstract: Airway remodeling is a main pathological characteristic of asthma, and strongly associated with migration and proliferation of airway smooth muscle cells. E-prostanoid (EP) receptor can regulate airway remodeling. This study established a rat model of asthma and evaluated EP changes in airway smooth muscle cells under the asthmatic state so as to provide theoretical evidence for developing EP drugs to treat airway remodeling in asthma. A total of 20 clean Sprague-Dawley rats were randomly assigned to asthma group and control group. 28 days later, they were sacrificed for histological examination. Airway smooth muscle cells were isolated, cultured and measured using quantitative fluorescent PCR. Histopathological examination revealed that rat models of asthma were in accordance with the manifestations of asthmatic airway remodeling. After reverse transcription, real-time quantitative fluorescent PCR was performed using miRNA Q-PCR diagnostic kit. GAPDH was considered the internal reference. Relative expressions of E-prostanoid 1–4 (EP1–4) (2-△△Ct) in the control and asthma groups were respectively as follows: EP1: 4.35±0.18, 6.55±1.21; EP2: 3.64±0.12, 1.35±1.06; EP3: 4.59±1.14, 5.89±1.74; EP4: 2.89±1.85, 1.69±0.44. EP2/4 significantly decreased, but EP1 significantly increased in the asthma group (P < 0.01). These results suggested that the reduced EP2/4 and increased EP1 expressions in airway smooth muscle cells of rat models of asthma were probably important factors for asthmatic airway remodeling.
Abstract: Airway remodeling is a main pathological characteristic of asthma, and strongly associated with migration and proliferation of airway smooth muscle cells. E-prostanoid (EP) receptor can regulate airway remodeling. This study established a rat model of asthma and evaluated EP changes in airway smooth muscle cells under the asthmatic state so as to p...
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Rhabdomyolysis Associated to Glossopharyngeal Edema: A Rare Side Effect of Diclofenac
Hicham Bakkali,
Lahcen Belyamani,
Salahedine Massou,
Loukmane Elwartiti,
Khalil Aboulaala,
Hicham Balkhi,
Charqui Haimeur
Issue:
Volume 2, Issue 6, November 2014
Pages:
161-164
Received:
21 November 2014
Accepted:
2 December 2014
Published:
15 December 2014
Abstract: Rhabdomyolysis is a widely used non steroid anti-inflammatory drug. Rhabdomyolysis after taking Diclofenac is rarely reported, and the association to a glossopharyngeal edema has never been discussed. Here is a case of rhabdomyolysis associated to glossopharyngeal edema after taking 200 mg of Diclofenac. The patient was first diagnosed with para phenyl di-amine intoxication but the toxicological examinations were negative. The patient outcome was good after symptomatic treatment.
Abstract: Rhabdomyolysis is a widely used non steroid anti-inflammatory drug. Rhabdomyolysis after taking Diclofenac is rarely reported, and the association to a glossopharyngeal edema has never been discussed. Here is a case of rhabdomyolysis associated to glossopharyngeal edema after taking 200 mg of Diclofenac. The patient was first diagnosed with para ph...
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Clinical Adverse Events Profile during Combination Therapy with Amlodipine and Hydrochlorothiazide in Hypertensive Nigerians
G. B. S. Iyalomhe,
E. K. I. Omogbai,
A. O. Isah,
S. I. Iyalomhe
Issue:
Volume 2, Issue 6, November 2014
Pages:
165-170
Received:
6 December 2014
Accepted:
16 December 2014
Published:
19 December 2014
Abstract: Background: Combination therapy with antihypertensive drugs from different classes has been recommended as an effective strategy to attain blood pressure (BP) goal. Although amlodipine (AML) and hydrochlorothiazide (HCZ) are frequently used as combination therapy in Nigeria, information regarding the adverse events associated with this regimen is scarce. Objective: To evaluate the clinical adverse events profile associated with a regimen of AML and HCZ therapy for 48 weeks (wks) in hypertensive Nigerians. Methods: Ninety male (M) and female (F) Nigerians aged 31-86 years with newly diagnosed uncomplicated essential hypertension (BP > 160/90 ≤ 180/120 mmHg), were enrolled. Patients, who were 30 each (15 Ms and 15 Fs) in AML, HCZ and AML-HCZ groups, were treated, respectively, with AML 5mg for 6 wks and the dose increased to 10mg till wk 12 (end of monotherapy) after which HCZ 25mg was added; HCZ 25mg till wk 6 (end of monotherapy) after which AML 5-10mg was added as needed; and AML 5-10mg + HCZ 25mg. Body mass index (BMI), BP and heart rate (HR) were assessed at baseline and at the end of wks 1, 3, 6, 12, 24, 36, and 48 during treatment. Adverse drug events were similarly monitored beginning from wk 1. Results: The three combination regimens comparably significantly reduced BP, though the effect in AML group was greater than the rest (P<0.05). Changes in BMI and HR were not statistically significant. Sixty one events of which polyuria, tachycardia and slight weight reduction were commonest as well as nausea and diaphoresis were recorded in AML group. Similarly, 91 events were observed in HCZ group, polyuria, tachycardia and weight loss being commonest plus impotence and visual disturbance. Although tachycardia and weight loss were more prominent among the 74 events in AML-HCZ group, polyuria was surprisingly uncommon. It is demonstrated that a regimen of AML to which HCZ is subsequently added provides superior tolerability and less bother to patients when compared with a regimen of HCZ to which AML is added as needed or with ab initio AML-HCZ combination therapy.
Abstract: Background: Combination therapy with antihypertensive drugs from different classes has been recommended as an effective strategy to attain blood pressure (BP) goal. Although amlodipine (AML) and hydrochlorothiazide (HCZ) are frequently used as combination therapy in Nigeria, information regarding the adverse events associated with this regimen is s...
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Management of Chronic Neuritis with an Extended and Continuous Course of Prednisolone and Methotrexate: A Case Report
Issue:
Volume 2, Issue 6, November 2014
Pages:
171-174
Received:
8 December 2014
Accepted:
17 December 2014
Published:
22 December 2014
Abstract: Patients may have prolonged neuritic episode in the field of leprosy. In that case, one or few interrupted courses of prednisolone of 4-months duration may prove inadequate for the unduly suffering patients. With an intention to see whether a prolonged course of prednisolone and methotrexate could be of any help for them, a patient having neuritis in several nerves including multiple abscesses in the right sural nerve was treated with a 15 months extended and continuous course of prednisolone and methotrexate. The outcome was complete and permanent remission with full functional recovery. Few mild self-limiting side effects from prednisolone were seen. However, there was no side effect from methotrexate. In conclusion, extended and continuous course of prednisolone and methotrexate was found safe and effective in treating a case of chronic neuritis.
Abstract: Patients may have prolonged neuritic episode in the field of leprosy. In that case, one or few interrupted courses of prednisolone of 4-months duration may prove inadequate for the unduly suffering patients. With an intention to see whether a prolonged course of prednisolone and methotrexate could be of any help for them, a patient having neuritis ...
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