Archive:: American Journal of Environmental Protection:: Science Publishing Group

Volume 9, Issue 2, April 2020

The Development and Application of DDPCR Technology on Quantification of Total Coliforms in Water

Wei Ma, Yi Jun Kong, Weng U Ho, Si Ian Lam, Gui Huan Liu, Sin Neng Chio

Issue: Volume 9, Issue 2, April 2020

Pages: 22-30

Received: 15 April 2020

Accepted: 30 April 2020

Published: 14 May 2020

DOI: 10.11648/j.ajep.20200902.11

Downloads:

Views:

Abstract: In this research, the detection method for absolute quantification of total coliforms was established based on Droplet Digital Polymerase Chain Reaction (DDPCR) technology using lacZ as the target gene for coliform group detection. The experimental conditions (e.g. primer and probe concentrations, annealing temperatures, etc) were well optimized. B... Show More

Download PDF

View Online
Threats and Conservation Strategies on Urban Wetlands: A Case of Monavale and Surrounding Areas in Harare, Zimbabwe

Silipiwe Sharai, Tarakini Tawanda, Chibememe Gladman

Issue: Volume 9, Issue 2, April 2020

Pages: 31-38

Received: 20 April 2020

Accepted: 5 May 2020

Published: 27 May 2020

DOI: 10.11648/j.ajep.20200902.12

Downloads:

Views:

Abstract: Wetlands are valuable ecosystems on which humans depend through the utilisation of the ecological goods and services including water for domestic and commercial use. Until recently, efforts on wetland conservation have shifted to community participation since they are important stakeholders in using wetland resources. This study solicited local peo... Show More

Download PDF

View Online