Identification of Differentially Expressed Genes During Pseudomonas fluorescens Mediated Systemic Resistance in Cabbage
Kaunain Roohie,
Sharanaiah Umesha
Issue:
Volume 3, Issue 4, July 2015
Pages:
30-36
Received:
10 September 2014
Accepted:
31 October 2014
Published:
14 July 2015
Abstract: The study of microbial ecology and the microbial interactions with plants provides an insight into the biocontrol of plant diseases using antagonistic microbes. Pseudomonas fluorescens was used as a biological control agent against black rot disease caused by Xanthomonas campestris pv. campestris. The Suppression subtractive hybridization (SSH) was used to elucidate the differentially expressed genes in cabbage (Brassica oleracea var. capitata) upon the application of Pseudomonas fluorescens. A total of 140 expressed sequence tags (EST) were obtained. The analyses of these ESTs showed that many defense related genes like peroxidase, heat shock proteins, were upregulated. Many transcripts related to signalling pathways and pathogen recognition were identified. The important finding of the study is the identification of the unigene belonging to the SWEET protein family in cabbage. The study also resulted in the identification of 10 unigenes which possibly depict the interaction of Pseudomonas fluorescens in combating disease. These unigenes have been submitted to dbEST. The results show that those genes which are upregulated during pathogen attack are also induced upon application of Pseudomonas fluorescens indicating the possible mechanism of systemic resistance induced by P. fluorescens to combat disease.
Abstract: The study of microbial ecology and the microbial interactions with plants provides an insight into the biocontrol of plant diseases using antagonistic microbes. Pseudomonas fluorescens was used as a biological control agent against black rot disease caused by Xanthomonas campestris pv. campestris. The Suppression subtractive hybridization (SSH) was...
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Selection of Salt Tolerant Somaclones for Development of Salt Stress Tolerant Varieties
Mst. Kohinoor Begum,
Md. Obaidul Islam
Issue:
Volume 3, Issue 4, July 2015
Pages:
37-46
Received:
28 June 2015
Accepted:
9 July 2015
Published:
18 July 2015
Abstract: The experiment were carried out at the farm of Bangladesh Sugarcane Research Institute Ishurdi, Pabna, Bangladesh during 2011-2012 cropping season. The somaclones which were produced in vitro under salinity stress evaluated in the field and after 1st year evaluation those which showed better performance were selected for this experiment to select salinity tolerant line of sugarcane. Sugarcane varieties Isd 28, Isd 35, Isd 36, Isd 37 and Isd 38 were used for selection of salinity tolerant lines. As treatments, salt were used with MS medium for in vitro plantlet regeneration. The selected somaclones were evaluated in 1st and 2nd year and after 1st year evaluation better somaclones were also evaluated under induced salinity stress conditions using different levels of salt (50 mM, 100 mM & 150 mM). Molecular characterizations were also done with the setts of selected somaclones collected from R2 generation. At the highest level of salinity (150 mM) varieties Isd 35 and Isd 37 initiated callus. Under induced salinity stress SC3 of Isd 28, SC3 of Isd 35 and SC2, SC3 of Isd 37 showed better performance in PVC pipe. Those somaclones also performed better in morpho-physiological performance at field condition in two sequential years and also produced polymorphic bands when we characterized with SSR marker. So those lines should be considered as salinity tolerant lines for further study.
Abstract: The experiment were carried out at the farm of Bangladesh Sugarcane Research Institute Ishurdi, Pabna, Bangladesh during 2011-2012 cropping season. The somaclones which were produced in vitro under salinity stress evaluated in the field and after 1st year evaluation those which showed better performance were selected for this experiment to select s...
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