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Determination of Beta-Lactamase Inactivation of Cephalexin by Validated RP-HPLC Method
Mahmoud Faysal Mshref,
Hesham Mohamed Ghonemy,
Amr Helmy Ali
Issue:
Volume 2, Issue 4, November 2017
Pages:
120-128
Received:
2 August 2017
Accepted:
26 August 2017
Published:
13 September 2017
DOI:
10.11648/j.wjac.20170204.11
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Abstract: Determination of the needed amount of liquid sterile Lactamator™ to be mixed to cephalexin, β-lactam antibiotic, for optimum deactivation of its molecule's antibacterial properties was conducted using RP-HPLC method. RP-HPLC method was validated for the parameters as linearity, accuracy, LOD, LOQ, and precision. Before the routine microbiological examination for any pharmaceutical dosage form containing β-lactam antibiotic, it is a must to make inactivate of β-lactam active pharmaceutical ingredient (API) by mixing with beta-lactamase before testing. So, the study indicated that mixing of 0.5 ml liquid sterile Lactamator™ with phosphate buffer solution pH (7.2) containing 50 mg cephalexin, with holding the test sample for 90 minutes prior to HPLC measurement will deactivation of cefalexin molecule's antibacterial properties.
Abstract: Determination of the needed amount of liquid sterile Lactamator™ to be mixed to cephalexin, β-lactam antibiotic, for optimum deactivation of its molecule's antibacterial properties was conducted using RP-HPLC method. RP-HPLC method was validated for the parameters as linearity, accuracy, LOD, LOQ, and precision. Before the routine microbiolog...
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Chemical Analysis of the Iron, Sodium, Potassium, Titanium and Aluminum Content of Kaolin Sample from Kankara, Kastina Nigeria
Zerendu Christian,
Okorn George,
Raji Saheed Akinleye,
Ibraheem Wasiu Aderemi
Issue:
Volume 2, Issue 4, November 2017
Pages:
129-133
Received:
14 August 2017
Accepted:
30 August 2017
Published:
30 September 2017
DOI:
10.11648/j.wjac.20170204.12
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Abstract: A total of 100 Clay samples was collected from Kankara Mengwa and Kankara Babangida and were analyzed for their Na, K, Fe, Al and Ti content using Instrumental Neutron Activation Analysis with Near Infra-Red analytical techniques. The result of the analysis hinted that the Kankara Mengwa kaolin is more qualitative than Kankara Babangida because of its low iron content, high aluminum content, low alkaline metal (Na and K) and low Ti content. It is envisaged that the findings of this research would provide substantial information that will trigger large scale industrial use of this clay.
Abstract: A total of 100 Clay samples was collected from Kankara Mengwa and Kankara Babangida and were analyzed for their Na, K, Fe, Al and Ti content using Instrumental Neutron Activation Analysis with Near Infra-Red analytical techniques. The result of the analysis hinted that the Kankara Mengwa kaolin is more qualitative than Kankara Babangida because of ...
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Determination of Tin in Trityl Candesartan by UV-VIS Spectrophotometer Using Phenylfluorone
Yusuf Yildiz,
Angela Jan,
Lokesh Pendyala,
Berrak Yildiz
Issue:
Volume 2, Issue 4, November 2017
Pages:
134-139
Received:
1 August 2017
Accepted:
16 August 2017
Published:
20 October 2017
DOI:
10.11648/j.wjac.20170204.14
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Abstract: Trityl Candesartan (TCand), a benzimidazole derivative, is widely used in the preparation of Candesartan Cilexitil, a pharmaceutical prodrug created to treat hypertension and to help lower blood pressure. One major concern in the preparation process is the usage of tin compounds, specifically tributyl tin oxide, which can cause harm to both human and environmental health. In this study, UV-VIS Spectrophotometry was used for the simple and accurate determination of tin (Sn) in Trityl Candesartan. The study of Sn in Trityl Candesartan found the Sn content to be within the limit of 10 mg/Kg, with a correlation coefficient (R2) of 0.9929, average percent recovery (%R) of 96.0%, and relative percentage difference (%RPD) of 0.35%.
Abstract: Trityl Candesartan (TCand), a benzimidazole derivative, is widely used in the preparation of Candesartan Cilexitil, a pharmaceutical prodrug created to treat hypertension and to help lower blood pressure. One major concern in the preparation process is the usage of tin compounds, specifically tributyl tin oxide, which can cause harm to both human a...
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Chemtaxonomic Relationship of Roots Phenolic Compounds for Selected Species of Four Families Recently Grouped in Brassicales by APGIII
Mubarak Siddig Hamad,
Ahmed Saeed Kabbashi,
Ikram Ahmed Madani
Issue:
Volume 2, Issue 4, November 2017
Pages:
140-144
Received:
14 August 2017
Accepted:
30 August 2017
Published:
2 November 2017
DOI:
10.11648/j.wjac.20170204.15
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Abstract: Chemotaxonomic relationship between five members recently grouped in the order Brassicales by APGIII were studied on the basis of their roots phenolic compounds constituents. The selected species are; Schimpera arabica from Brassicaceae, Maerua crassifolia and Maerua oblongifolia from Capparacea, Cleome gynandra from Cleomaceae and Salvadora persica from Salvadoraceae. Roots were extracted in ethanol (80%). Phenolic compounds were separated using Thin Layer Chromatography (TLC) technique. The developing solvent which is a mixture of Toluene- Chlorophorm – Acetone (in a rate of 10:50:7). The paired affinity between the species was constructed base on roots phenolic compounds, most of the species revealed closeness but The highest paired affinity was observed between Schimperaarabica and Maeruaoblongifolia. Polygonal graphs were constructed on the basis of the paired affinity which reflects the obvious closeness between studied species. This investigations Provided evidence that support grouping the selected species in the order Brassicales. More chemicals investigation for different members and different parts of the order will be better to demonstrate their closeness.
Abstract: Chemotaxonomic relationship between five members recently grouped in the order Brassicales by APGIII were studied on the basis of their roots phenolic compounds constituents. The selected species are; Schimpera arabica from Brassicaceae, Maerua crassifolia and Maerua oblongifolia from Capparacea, Cleome gynandra from Cleomaceae and Salvadora persic...
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Preliminary Phytochemical Screening of Five Commercial Essential Oils
Bachir Raho Ghalem,
Bellil Ali
Issue:
Volume 2, Issue 4, November 2017
Pages:
145-151
Received:
4 August 2017
Accepted:
4 September 2017
Published:
6 December 2017
DOI:
10.11648/j.wjac.20170204.16
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Abstract: The discovery of natural resources from plants remains crucial for the development of new therapeutic remedies. The present study focused on the phytochemical study of commercial essential oils of certain medicinal plants: Thym (Thymus vulgaris), basil (Ocimum basilicum), sage (Salvia officinalis), Garden cress (Lepidium sativum) and lettuce (Lactuca sativa). The qualitative dosage of twelve secondary metabolites showed the presence of terpenoids and saponins in all tested oils while steroids, alkaloids, anthraquinones and phlobatannins were absent. The results obtained are encouraging and the secondary metabolites present in these oils were responsible for certain activities, hence the interest of their uses in traditional phytotherapy as well as the pharmacological and cosmetic field.
Abstract: The discovery of natural resources from plants remains crucial for the development of new therapeutic remedies. The present study focused on the phytochemical study of commercial essential oils of certain medicinal plants: Thym (Thymus vulgaris), basil (Ocimum basilicum), sage (Salvia officinalis), Garden cress (Lepidium sativum) and lettuce (Lactu...
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