Abstract: Object: To investigate the inhibitory effect of HMME-PDT on the cariogenic bacteria we designed different photosensitizer concentration and different lighting time. Therefore, we determined the optimal photosensitizer parameters and time parameters. Besides we explored the advantages of HMME-PDT in inhibiting the cariogenic bacteria in dental plaque biofilms of rats. We provided theoretical and experi- mental basis for the application of PDT for dental caries prevention. Method: The caries model was constructed with Wistar rats. We grouped the rats in accordance with the principle of randomization. We used the above two methods to evaluate the inhibitory effect of HMME-PDT on the cariogenic bacteria in dental plaque biofilms of rats. Results: 1. The bacteriostatic effect of HMME-PDT on bacterial plaque in rat oral cavity was studied in different concentration of photosensitizer. The tablet colony count results showed with the increase of concentration of photosensitizer stress the bacteria inhibitive rate was increased. Compared to the negative control group and positive control group, group E1 changed most obviously. 2. The bacteriostatic effect of HMME-PDT on bacterial plaque in rat oral cavity was studied in different exposure time. The tablet colony count results showed with the increase of exposure time the bacteria inhibitive rate was increased. Compared to the negative control group and positive control group, group E2 changed most obviously. 3. Results of the calcium ion dissolution detected by atomic absorption spectrophotometer showed with the increase of exposure time the calcium ion dissolution was increased in each group in 48 hours. Compared to the negative control group, the calcium ion dissolution of each group was decreased significantly (P<0.05). Compared to the positive control group, the calcium ion dissolution of each group was decreased significantly (P<0.05). Conclusion: Photodynamic therapy can effectively inhibit the production of caries in the biofilm of rats and reduce calcium ion solubility in early caries.Abstract: Object: To investigate the inhibitory effect of HMME-PDT on the cariogenic bacteria we designed different photosensitizer concentration and different lighting time. Therefore, we determined the optimal photosensitizer parameters and time parameters. Besides we explored the advantages of HMME-PDT in inhibiting the cariogenic bacteria in dental plaqu...Show More
