In-vitro Antimicrobial, Anti-oxidant Activities and Cytotoxicty of Carum carvi L
Mohamed N. Abdalaziz,
Mahmoud Mohamed Ali,
Mohamed I. Garbi,
Mohammed Abdalbagi Dafalla,
Ahmed S. Kabbashi
Issue:
Volume 3, Issue 3, June 2017
Pages:
23-27
Received:
28 February 2017
Accepted:
19 April 2017
Published:
25 July 2017
Abstract: Carum carvi L. was used traditionally in different populations for many medical complains. The study was aimed to investigate antimicrobial, anti-oxidant activities and cytotoxicty of fixed oil of Carum carvi L. (seeds). The oil was extraction by petroleum ether (60-80°C) using a Soxhlet apparatus. The oil of Carum carvi L. seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the disc diffusion method. The antioxidant activities were conducted via DPPH radical scavenging assay and cytotoxicty using brine shrimp assay. Antimicrobial activity of fixed oil of C. carvi L. dissolved in methanol (1:10), showed high activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (18 & 14 mm). It also showed against Gram positive bacteria (S. aureus & B.subtilis) (14 & 13 mm) and against (C. albicans) (14 mm). The tested anti-oxidant activity gave (18±0.06 RSA %) in comparison to the control of propylgalate (92±0.01 RSA %). In addition cytotoxicity (brine shrimp lethality Bioassay) verified the safety of the examined extract with an IC50 less than 1000μg/ml. This study conducted for essential oil of C. carvi L. seeds proved to have potent activities against antimicrobial activity In-vitro with verified safety evidence for use.
Abstract: Carum carvi L. was used traditionally in different populations for many medical complains. The study was aimed to investigate antimicrobial, anti-oxidant activities and cytotoxicty of fixed oil of Carum carvi L. (seeds). The oil was extraction by petroleum ether (60-80°C) using a Soxhlet apparatus. The oil of Carum carvi L. seeds were tested agains...
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Characterization of Oil Extracted from Two Varieties of Tiger Nut (Cyperus esculentus L.) Tubers
Aliyu Ahmad Warra,
Leye Jonathan Babatola,
Adedara Adejoju Omodolapo,
Basiru Dende Ibraheem
Issue:
Volume 3, Issue 3, June 2017
Pages:
28-36
Received:
16 May 2017
Accepted:
12 July 2017
Published:
27 July 2017
Abstract: The qualitative determination of fatty acids from hexane extract of two varieties of Cyperus esculentus L. tuber oil using Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed the following fatty acids; palmitic acid, steric acid, Margaric acid, elaidic acid, oleic acid, erucic acid, behenic acid. and arachidic acid. For the Fourier Transform Infra-Red (FT-IR) analysis, bands of 3469.09 cm-1, 3463.3 cm-1, sharp bands at 2867.28 cm-1, 2037.86 cm-1 and 2866.32 cm-1 asymmetrical and symmetrical modes of vibration of –CH2-, strong band 1742.74 cm-1 and 1743.71 cm-1 due to ester carbonyl functional group of the triacylglycerols, bending vibrations of the CH2 and CH3 aliphatic groups,(C-CO-O- and O-CH2-C) and the in-planebending vibration of CH cis-olefinic groups seen at 1449.55 cm-1, 1364 cm-1 and 1450.52 cm-1, 1365.65, and 1450.52 cm-1, 1365.65 cm-1, vibration frequency at, 1166.97 cm-1 finger print of the stretching vibration of the C-O ester group, frequencies of the in-and out-of plane rocking of the cis-olefinic CH2 group at 718.51 cm-1 were observed for the brown and yellow tiger nut tubers oil respectively. The results were in favour of the utilization of the two varieties of Tiger nut (Cyperus esculentus L.) tubers oil in cosmetics, polymer and food industries.
Abstract: The qualitative determination of fatty acids from hexane extract of two varieties of Cyperus esculentus L. tuber oil using Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed the following fatty acids; palmitic acid, steric acid, Margaric acid, elaidic acid, oleic acid, erucic acid, behenic acid. and arachidic acid. For the Fourier Trans...
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