Phytochemical and Anti-Microbial Analysis of the Roots of Ficus Exasparata (Anwirinwa)
Onyema,
Chukwuebuka ThankGod,
Ajiwe,
Vincent Ishmael,
Nwankwo,
Ogochukwu Obioma
Issue:
Volume 2, Issue 3, June 2014
Pages:
11-16
Received:
20 June 2014
Accepted:
3 July 2014
Published:
10 July 2014
Abstract: The roots of Ficus exasparata of the Moraceae family were duly analysed to ascertain the active constituents responsible for the use of the plant part in the treatment of inflammatory diseases, treatment of internal bleeding, bruises, cough and dangerous boils among others. The phytochemical analysis showed the presence of some secondary metabolites in various concentrations with saponin and alkaloids present in low concentration while reducing sugars were present in very high concentration with protein, carbohydrates and acidic components totally absent. The Atomic Absorption Spectroscopic (AAS) analysis showed the presence of certain trace elements such as Cd (0.55ppm), Cr (1.88ppm),As (0.38ppm), Hg (0.09ppm) etc which were higher than the WHO recommended standard of Cd (0.003ppm), Cr (0.05ppm), As (0.01ppm), Hg (0.001ppm) respectively an observation that could be attributed to the crude nature of the plant parts. Three different choice solvents (Chloroform, Ethylacetate and Chloroform-Methanol mixture) were used in the extraction of the plant part. The extracts were subsequently subjected to thin layer chromatography with respective Rf values as 0.13, 0.22 and 0.60 respectively showing just a single spot each for all the solvents used. The extracts were subjected to antimicrobial screening using eight pathogenic bacteria species and three fungi with only the chloroform extract exhibiting activity on the selected test organisms. The three extracts from the sample were subjected to structural elucidation using a combination of some spectroscopic techniques such as FTIR, UV-VIS, H1-NMR, C13-NMR and GCMS. The spectral analysis suggested the presence of Coumarin-3-carboxamide, 8-allyl-N-(3-nitrophenyl) and 1,2,3-propanetricarboxylic acid, 2-(acetyloxy)-tributyl ester for chloroform and ethyl acetate extracts respectively. The constituents of these extracts such as Coumarin in chloroform extract could account for its potency in curing certain illnesses as coumarin is a well known natural product that has displayed a broad range of biological activities.
Abstract: The roots of Ficus exasparata of the Moraceae family were duly analysed to ascertain the active constituents responsible for the use of the plant part in the treatment of inflammatory diseases, treatment of internal bleeding, bruises, cough and dangerous boils among others. The phytochemical analysis showed the presence of some secondary metabolite...
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Synthesis, Magnetic, Spectral and Biological Studies of Copper(II) Complexes of 4-Benzoyl-3-Methyl-1-Phenyl-2-Pyrazolin-5-One N(4)-Substituted Thiosemicarbazones
Ayman K. El-Sawaf,
Amal Abdel Fatah Nassar,
El-Sayed El-Samanody
Issue:
Volume 2, Issue 3, June 2014
Pages:
17-26
Received:
11 August 2014
Accepted:
18 August 2014
Published:
30 August 2014
Abstract: The synthesis and characterization of copper(II) complexes with 4-benzoyl-3-methyl-1-phenyl-2-pyrazolin-5-one thiosemicarbazone and N(4)-substituted thiosemicarbazones are reported. Elemental analysis, molar conductivities, magnetic measurements and spectral (i.r., electronic, e.s.r., and n.m.r.) studies have been used to characterize the complexes. The i.r. spectra show that the thiosemicarbazones behave as bidentate ligands, either in the thione or thiolato form. Stereochemistries are proposed for the complexes on the basis of spectral and magnetic studies. Both the thiosemicarbazones and their complexes show either modest or no growth inhibitory activity against Aspergillus niger and Paecilomyces variotii.
Abstract: The synthesis and characterization of copper(II) complexes with 4-benzoyl-3-methyl-1-phenyl-2-pyrazolin-5-one thiosemicarbazone and N(4)-substituted thiosemicarbazones are reported. Elemental analysis, molar conductivities, magnetic measurements and spectral (i.r., electronic, e.s.r., and n.m.r.) studies have been used to characterize the complexes...
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