Genetic Characterization of Shiga Toxin-producing Escherichia coli Strains Isolated from Imported Beef Meat in Malaysia Using Polymerase Chain Reaction Analysis
Abuelhassan Nawal Noureldaim,
Sahilah Abdul Mutalib,
Gimba Fufa Ido,
Yusoff Wan Mohtar
Issue:
Volume 5, Issue 4, December 2019
Pages:
52-72
Received:
21 October 2019
Accepted:
15 November 2019
Published:
6 December 2019
Abstract: This work was to genetically characterized Shiga toxin-producing Escherichia coli strains isolated from imported beef meat in Malaysia using polymerase chain reaction (PCR) base analysis, seventy four (74) frozen beef meats samples (imported) (n=74) tested originated forty two of the sample were from India and thirty two of the samples were from Australia. The samples were bought from the frozen meat units of five different supermarkets in diverse settings in Malaysia which start from 2012 April to 2013 October on a weekly basis. E. coli and shiga toxin producing E. coli isolation was done Isolation of plasmid was carried out using the PureYield™ Plasmid Miniprep System. Enterobacterial Repetitive Intergenic Consensus (ERIC) primer was use in genetically isolating the bacteria: We used seven types of primers for Random Amplified Polymorphic DNA-PCR (RAPD-PCR) namely Gen8, Gen9, A1, A7, A10, OPAR8 and OPAR20. Our result revealed that Plasmid profiling showed 16 patterns based on size and 4 patterns on gel. Combination of phenotypically and genotypically approaches revealed a varied heterogeneity among imported beef isolates of E. coli. Isolated plasmids from shiga toxin producing E. coli varied in sizes. The sizes are from 4.3 -23.1 kilo base (kb). Sixty two (62) isolates were found to harbor plasmid with size 23.2 kb while 43 isolates harbored more than a single plasmid. The analysis of data through the use of average linkage (UPGMA, unweight group pair technique with arithmetic averages) using Gel compare 11 software which was displayed in dendrograms, for the, ERIC-PCR and RAPD-PCR (RAPD Gen 8, Gen 9), (Opar 8 and Opar 20), (RAPD A1, RAPD A7and RAPD A10) analysis, produced various cluster. The PCR analysis using OPAR 8 and OPAR 20 also produces various clusters.
Abstract: This work was to genetically characterized Shiga toxin-producing Escherichia coli strains isolated from imported beef meat in Malaysia using polymerase chain reaction (PCR) base analysis, seventy four (74) frozen beef meats samples (imported) (n=74) tested originated forty two of the sample were from India and thirty two of the samples were from Au...
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Mother’s Behavior in Treating of Acute Respiratory Infections on Children Post the Earthquake and Tsunami Disaster at the Public Health Center in Wani, Donggala, Central of Sulawesi, Indonesia
Rasyika Nurul Fadjriah,
Herman,
St Ika Fitrasyah,
Sadly Syam,
Firmansyah,
Sendhy Krisnasari,
Debby Trisha,
Rosmala Nur,
Rahma Dwi Larasati,
Elvaria Mantao
Issue:
Volume 5, Issue 4, December 2019
Pages:
73-77
Received:
2 December 2019
Accepted:
12 December 2019
Published:
24 December 2019
Abstract: Background: After disaster happened, the number of ARI (Acute Respiratory Infections) cases could dramatically increase and even ranked top. The public health center (Puskesmas) in Wani is the public health facility that has the highest number of child ARI patients in Donggala Regency after the disaster happened. The public health center handled 149 ARI cases from the total of 1,597 cases occurred. This study was aimed to find out the knowledge and behavior of mothers, facilities, health services, health worker and family support for mothers that the children suffered from ARI at the working area of Wani public health center. Methods: This study is a qualitative research using case study approach. The data were collected through in-depth interviews with 12 informants who were selected through purposive sampling technique. Results: The results of the research showed that mothers’ knowledgeable on ARI was still low due to the lack of information. Their behavior related to ARI management was good that was showed by their decision to take immediate medical treatment for their children by buying them drugs and taking them to health facilities. However, there were also mothers decided to give their children self-medication (traditional) that was performed at home by buying self-prescribed medication or even using traditional medicines. The health facilities are adequate to be utilized by the mothers. However, the role of health worker has not been maximized because of the lack of information related to ARI. Some informants got social support from their families in the form of information and emotional supports, but there were also some informants who did not get adequate support from their families. Conclusion. The low level of mothers’ knowledge and lack of social support from health workers influenced the mother’s behavior in treating of ARI on children at Wani Public Health Center.
Abstract: Background: After disaster happened, the number of ARI (Acute Respiratory Infections) cases could dramatically increase and even ranked top. The public health center (Puskesmas) in Wani is the public health facility that has the highest number of child ARI patients in Donggala Regency after the disaster happened. The public health center handled 14...
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Assessment of Microbiological Quality of Air Environment Around Waste Dumpsites Within Keffi Metropolis in Northern Nigeria
Obiekezie Smart Obumneme,
Ekeleme Ike Kenneth,
Adamu Muktar Owuna,
Gnimintakpa M’bouyowe Joseph,
Onalo Cornelius and Sabo,
Abdullahi Ado
Issue:
Volume 5, Issue 4, December 2019
Pages:
78-84
Received:
30 November 2019
Accepted:
16 December 2019
Published:
24 December 2019
Abstract: This study is to assess the microbiological quality and public health hazard associated with the air environment around waste dumpsites in Keffi, metropolis Nigeria was conducted. A total of 25 samples were collected from five different waste dumpsites around five different locations within the metropolis (Angwan Lambu, Keffi Main market, University Main campus and Nasarawa Road). Preliminary cultural, morphological, biochemical characteristics of the isolates revealed the following isolates, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Proteus vulgaris, Bacillus spp, Micrococcus spp., Aspergillus niger, Alternaria spp., Penicillum spp., Mucor spp., Fusarium spp., Rhizopus stolonifer and Candida spp. The total bacterial counts from the air environments of these waste dumpsites ranged between 2.2-7.4 x 101 cfu/m3, while the total fungal counts ranged between 1.4-4.4 x 101 cfu/m3. The susceptibility of the bacterial isolates against commonly used antibiotics tested showed varying degree of susceptibility and it is in the following decreasing order; Proteus vulgaris (93.3%) > Staphylococcus aureus (55.6%) > Micrococcus spp. (43.3%) > Pseudomonas aeruginosa (42.8%) > Bacillus spp. (41.2%) > Escherichia coli (30.8%). Health risk assessment indicates the presence of pathogenic bacteria and moulds with serious health risk to human within the environment due to the possibility of cross contamination of foods sold in the open. Consequently, there is the need to periodically monitor the number of microorganisms within the air of these environments using the established threshold stipulated for microbial concentrations in air.
Abstract: This study is to assess the microbiological quality and public health hazard associated with the air environment around waste dumpsites in Keffi, metropolis Nigeria was conducted. A total of 25 samples were collected from five different waste dumpsites around five different locations within the metropolis (Angwan Lambu, Keffi Main market, Universit...
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