Genetic Characterization of Shiga Toxin-producing <i>Escherichia coli</i> Strains Isolated from Imported Beef Meat in Malaysia Using Polymerase Chain Reaction Analysis

Abuelhassan Nawal Noureldaim; Sahilah Abdul Mutalib; Gimba Fufa Ido; Yusoff Wan Mohtar

doi:doi:10.11648/j.ajbes.20190504.11

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Genetic Characterization of Shiga Toxin-producing Escherichia coli Strains Isolated from Imported Beef Meat in Malaysia Using Polymerase Chain Reaction Analysis

Abuelhassan Nawal Noureldaim, Sahilah Abdul Mutalib, Gimba Fufa Ido, Yusoff Wan Mohtar

Published in American Journal of Biological and Environmental Statistics (Volume 5, Issue 4)

Received: 21 October 2019 Accepted: 15 November 2019 Published: 6 December 2019

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Abstract

This work was to genetically characterized Shiga toxin-producing Escherichia coli strains isolated from imported beef meat in Malaysia using polymerase chain reaction (PCR) base analysis, seventy four (74) frozen beef meats samples (imported) (n=74) tested originated forty two of the sample were from India and thirty two of the samples were from Australia. The samples were bought from the frozen meat units of five different supermarkets in diverse settings in Malaysia which start from 2012 April to 2013 October on a weekly basis. E. coli and shiga toxin producing E. coli isolation was done Isolation of plasmid was carried out using the PureYield™ Plasmid Miniprep System. Enterobacterial Repetitive Intergenic Consensus (ERIC) primer was use in genetically isolating the bacteria: We used seven types of primers for Random Amplified Polymorphic DNA-PCR (RAPD-PCR) namely Gen8, Gen9, A1, A7, A10, OPAR8 and OPAR20. Our result revealed that Plasmid profiling showed 16 patterns based on size and 4 patterns on gel. Combination of phenotypically and genotypically approaches revealed a varied heterogeneity among imported beef isolates of E. coli. Isolated plasmids from shiga toxin producing E. coli varied in sizes. The sizes are from 4.3 -23.1 kilo base (kb). Sixty two (62) isolates were found to harbor plasmid with size 23.2 kb while 43 isolates harbored more than a single plasmid. The analysis of data through the use of average linkage (UPGMA, unweight group pair technique with arithmetic averages) using Gel compare 11 software which was displayed in dendrograms, for the, ERIC-PCR and RAPD-PCR (RAPD Gen 8, Gen 9), (Opar 8 and Opar 20), (RAPD A1, RAPD A7and RAPD A10) analysis, produced various cluster. The PCR analysis using OPAR 8 and OPAR 20 also produces various clusters.

Published in	American Journal of Biological and Environmental Statistics (Volume 5, Issue 4)
DOI	10.11648/j.ajbes.20190504.11
Page(s)	52-72
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Genetic Characterization, Shiga Toxin-producing Escherichia coli, Imported Beef Meat, Polymerase Chain Reaction (PCR) and Malaysia

References

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APA Style

Abuelhassan Nawal Noureldaim, Sahilah Abdul Mutalib, Gimba Fufa Ido, Yusoff Wan Mohtar. (2019). Genetic Characterization of Shiga Toxin-producing Escherichia coli Strains Isolated from Imported Beef Meat in Malaysia Using Polymerase Chain Reaction Analysis. American Journal of Biological and Environmental Statistics, 5(4), 52-72. https://doi.org/10.11648/j.ajbes.20190504.11

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Abuelhassan Nawal Noureldaim; Sahilah Abdul Mutalib; Gimba Fufa Ido; Yusoff Wan Mohtar. Genetic Characterization of Shiga Toxin-producing Escherichia coli Strains Isolated from Imported Beef Meat in Malaysia Using Polymerase Chain Reaction Analysis. Am. J. Biol. Environ. Stat. 2019, 5(4), 52-72. doi: 10.11648/j.ajbes.20190504.11

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AMA Style

Abuelhassan Nawal Noureldaim, Sahilah Abdul Mutalib, Gimba Fufa Ido, Yusoff Wan Mohtar. Genetic Characterization of Shiga Toxin-producing Escherichia coli Strains Isolated from Imported Beef Meat in Malaysia Using Polymerase Chain Reaction Analysis. Am J Biol Environ Stat. 2019;5(4):52-72. doi: 10.11648/j.ajbes.20190504.11

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@article{10.11648/j.ajbes.20190504.11,
  author = {Abuelhassan Nawal Noureldaim and Sahilah Abdul Mutalib and Gimba Fufa Ido and Yusoff Wan Mohtar},
  title = {Genetic Characterization of Shiga Toxin-producing Escherichia coli Strains Isolated from Imported Beef Meat in Malaysia Using Polymerase Chain Reaction Analysis},
  journal = {American Journal of Biological and Environmental Statistics},
  volume = {5},
  number = {4},
  pages = {52-72},
  doi = {10.11648/j.ajbes.20190504.11},
  url = {https://doi.org/10.11648/j.ajbes.20190504.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbes.20190504.11},
  abstract = {This work was to genetically characterized Shiga toxin-producing Escherichia coli strains isolated from imported beef meat in Malaysia using polymerase chain reaction (PCR) base analysis, seventy four (74) frozen beef meats samples (imported) (n=74) tested originated forty two of the sample were from India and thirty two of the samples were from Australia. The samples were bought from the frozen meat units of five different supermarkets in diverse settings in Malaysia which start from 2012 April to 2013 October on a weekly basis. E. coli and shiga toxin producing E. coli isolation was done Isolation of plasmid was carried out using the PureYield™ Plasmid Miniprep System. Enterobacterial Repetitive Intergenic Consensus (ERIC) primer was use in genetically isolating the bacteria: We used seven types of primers for Random Amplified Polymorphic DNA-PCR (RAPD-PCR) namely Gen8, Gen9, A1, A7, A10, OPAR8 and OPAR20. Our result revealed that Plasmid profiling showed 16 patterns based on size and 4 patterns on gel. Combination of phenotypically and genotypically approaches revealed a varied heterogeneity among imported beef isolates of E. coli. Isolated plasmids from shiga toxin producing E. coli varied in sizes. The sizes are from 4.3 -23.1 kilo base (kb). Sixty two (62) isolates were found to harbor plasmid with size 23.2 kb while 43 isolates harbored more than a single plasmid. The analysis of data through the use of average linkage (UPGMA, unweight group pair technique with arithmetic averages) using Gel compare 11 software which was displayed in dendrograms, for the, ERIC-PCR and RAPD-PCR (RAPD Gen 8, Gen 9), (Opar 8 and Opar 20), (RAPD A1, RAPD A7and RAPD A10) analysis, produced various cluster. The PCR analysis using OPAR 8 and OPAR 20 also produces various clusters.},
 year = {2019}
}

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TY - JOUR
T1 - Genetic Characterization of Shiga Toxin-producing Escherichia coli Strains Isolated from Imported Beef Meat in Malaysia Using Polymerase Chain Reaction Analysis
AU - Abuelhassan Nawal Noureldaim
AU - Sahilah Abdul Mutalib
AU - Gimba Fufa Ido
AU - Yusoff Wan Mohtar
Y1 - 2019/12/06
PY - 2019
N1 - https://doi.org/10.11648/j.ajbes.20190504.11
DO - 10.11648/j.ajbes.20190504.11
T2 - American Journal of Biological and Environmental Statistics
JF - American Journal of Biological and Environmental Statistics
JO - American Journal of Biological and Environmental Statistics
SP - 52
EP - 72
PB - Science Publishing Group
SN - 2471-979X
UR - https://doi.org/10.11648/j.ajbes.20190504.11
AB - This work was to genetically characterized Shiga toxin-producing Escherichia coli strains isolated from imported beef meat in Malaysia using polymerase chain reaction (PCR) base analysis, seventy four (74) frozen beef meats samples (imported) (n=74) tested originated forty two of the sample were from India and thirty two of the samples were from Australia. The samples were bought from the frozen meat units of five different supermarkets in diverse settings in Malaysia which start from 2012 April to 2013 October on a weekly basis. E. coli and shiga toxin producing E. coli isolation was done Isolation of plasmid was carried out using the PureYield™ Plasmid Miniprep System. Enterobacterial Repetitive Intergenic Consensus (ERIC) primer was use in genetically isolating the bacteria: We used seven types of primers for Random Amplified Polymorphic DNA-PCR (RAPD-PCR) namely Gen8, Gen9, A1, A7, A10, OPAR8 and OPAR20. Our result revealed that Plasmid profiling showed 16 patterns based on size and 4 patterns on gel. Combination of phenotypically and genotypically approaches revealed a varied heterogeneity among imported beef isolates of E. coli. Isolated plasmids from shiga toxin producing E. coli varied in sizes. The sizes are from 4.3 -23.1 kilo base (kb). Sixty two (62) isolates were found to harbor plasmid with size 23.2 kb while 43 isolates harbored more than a single plasmid. The analysis of data through the use of average linkage (UPGMA, unweight group pair technique with arithmetic averages) using Gel compare 11 software which was displayed in dendrograms, for the, ERIC-PCR and RAPD-PCR (RAPD Gen 8, Gen 9), (Opar 8 and Opar 20), (RAPD A1, RAPD A7and RAPD A10) analysis, produced various cluster. The PCR analysis using OPAR 8 and OPAR 20 also produces various clusters.
VL - 5
IS - 4
ER -

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Author Information

Abuelhassan Nawal Noureldaim

Department of Food Science, Faculty of Food Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Malaysia
Sahilah Abdul Mutalib

Department of Food Science, Faculty of Food Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Malaysia
Gimba Fufa Ido

Department of Pathology and Microbiology, Faculty of Veterinary Medicine, University Putra Malaysia, Selangor, Malaysia
Yusoff Wan Mohtar

Institute Alam Dan Tamdun Melayu, Universiti Kebangsaan Malaysia, Bangi, Malaysia

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Abuelhassan Nawal Noureldaim, Sahilah Abdul Mutalib, Gimba Fufa Ido, Yusoff Wan Mohtar. (2019). Genetic Characterization of Shiga Toxin-producing Escherichia coli Strains Isolated from Imported Beef Meat in Malaysia Using Polymerase Chain Reaction Analysis. American Journal of Biological and Environmental Statistics, 5(4), 52-72. https://doi.org/10.11648/j.ajbes.20190504.11

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Abuelhassan Nawal Noureldaim; Sahilah Abdul Mutalib; Gimba Fufa Ido; Yusoff Wan Mohtar. Genetic Characterization of Shiga Toxin-producing Escherichia coli Strains Isolated from Imported Beef Meat in Malaysia Using Polymerase Chain Reaction Analysis. Am. J. Biol. Environ. Stat. 2019, 5(4), 52-72. doi: 10.11648/j.ajbes.20190504.11

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AMA Style

Abuelhassan Nawal Noureldaim, Sahilah Abdul Mutalib, Gimba Fufa Ido, Yusoff Wan Mohtar. Genetic Characterization of Shiga Toxin-producing Escherichia coli Strains Isolated from Imported Beef Meat in Malaysia Using Polymerase Chain Reaction Analysis. Am J Biol Environ Stat. 2019;5(4):52-72. doi: 10.11648/j.ajbes.20190504.11

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@article{10.11648/j.ajbes.20190504.11,
  author = {Abuelhassan Nawal Noureldaim and Sahilah Abdul Mutalib and Gimba Fufa Ido and Yusoff Wan Mohtar},
  title = {Genetic Characterization of Shiga Toxin-producing Escherichia coli Strains Isolated from Imported Beef Meat in Malaysia Using Polymerase Chain Reaction Analysis},
  journal = {American Journal of Biological and Environmental Statistics},
  volume = {5},
  number = {4},
  pages = {52-72},
  doi = {10.11648/j.ajbes.20190504.11},
  url = {https://doi.org/10.11648/j.ajbes.20190504.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbes.20190504.11},
  abstract = {This work was to genetically characterized Shiga toxin-producing Escherichia coli strains isolated from imported beef meat in Malaysia using polymerase chain reaction (PCR) base analysis, seventy four (74) frozen beef meats samples (imported) (n=74) tested originated forty two of the sample were from India and thirty two of the samples were from Australia. The samples were bought from the frozen meat units of five different supermarkets in diverse settings in Malaysia which start from 2012 April to 2013 October on a weekly basis. E. coli and shiga toxin producing E. coli isolation was done Isolation of plasmid was carried out using the PureYield™ Plasmid Miniprep System. Enterobacterial Repetitive Intergenic Consensus (ERIC) primer was use in genetically isolating the bacteria: We used seven types of primers for Random Amplified Polymorphic DNA-PCR (RAPD-PCR) namely Gen8, Gen9, A1, A7, A10, OPAR8 and OPAR20. Our result revealed that Plasmid profiling showed 16 patterns based on size and 4 patterns on gel. Combination of phenotypically and genotypically approaches revealed a varied heterogeneity among imported beef isolates of E. coli. Isolated plasmids from shiga toxin producing E. coli varied in sizes. The sizes are from 4.3 -23.1 kilo base (kb). Sixty two (62) isolates were found to harbor plasmid with size 23.2 kb while 43 isolates harbored more than a single plasmid. The analysis of data through the use of average linkage (UPGMA, unweight group pair technique with arithmetic averages) using Gel compare 11 software which was displayed in dendrograms, for the, ERIC-PCR and RAPD-PCR (RAPD Gen 8, Gen 9), (Opar 8 and Opar 20), (RAPD A1, RAPD A7and RAPD A10) analysis, produced various cluster. The PCR analysis using OPAR 8 and OPAR 20 also produces various clusters.},
 year = {2019}
}

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