@article{10.11648/j.pst.20220601.12,
  author = {Esha Sarkar and Anchal Trivedi and Aparna Misra and Anil Kumar Balapure},
  title = {In Vitro Effect of Chlorquine and Picrolive on DNA Content and Protein-Turnover in Liver and Spleen Cultures After Plasmodium Berghei Infection},
  journal = {Pharmaceutical Science and Technology},
  volume = {6},
  number = {1},
  pages = {6-25},
  doi = {10.11648/j.pst.20220601.12},
  url = {https://doi.org/10.11648/j.pst.20220601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.pst.20220601.12},
  abstract = {Background: Malaria is the major cause of disease and death on a global basis. The situation has worsened due to the emergence of parasite strains, resistant to a number of known antimalarials, causing relapse mostly in case of P vivax. That’s why the great need of combination drug therapy along with known compounds and phytocompound came into the picture for dealing drug resistance by introducing a new therapeutic approach. As liver and spleen are found to play the major roles to overcome the complications during malarial parasitemia, we have focused on the alteration of DNA synthesis and protein content during different levels of parasitemia before and after drug application. The study aims to analyse of the activity of Chloroquine and Picroliv on DNA content and protein turn over in both liver and spleen explants culture after P. berghei infection. Histological preparation of spleen explants for paraplast embedding total DNA content and protein turnover. In liver: In malarial, DNA content is observed to decline rapidly as the parasitemia level increases in liver tissue culture. Decrement of DNA content was found almost 25-52% as the parasitemia level increases 60% up to 0-4 days of culture. After insulin supplementation DNA content was found to increase almost 23% on Day 2 and 33% on day 4. Addition of picroliv enhances DNA content about 32%, 11% on day 2 and 4 respectively. Effect of Chloroquine is also observed with insulin and picroliv administration. Throughout the process, total protein concentration was observed to get decreased in proportion to the rate of infection. At 23% of infection protein values decreased by about 4 of culture respectively over the normal controls. Rate of 3-H-Thymidine incorporation is also observed at different stages of parasitemia. Spleen: In spleen also the same tests were done to observe DNA content at different parasitemia levels, where the reduction of DNA content was observed at 60% infection to be as 11% on day3. After addition of insulin it was increased by 133%; infections. Further increase was also observed about 94%. Total protein concentration was also observed to decrease by 23%, 66% at 20% parasitaemia on day 0 and 3. Total DNA content and total protein content was observed to decrease as the parasitemia level increases in cases of liver and spleen. After addition of insulin, could increase the DNA content slightly, further enhanced by application of picroliv in the media.},
 year = {2022}
}

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TY  - JOUR
T1  - In Vitro Effect of Chlorquine and Picrolive on DNA Content and Protein-Turnover in Liver and Spleen Cultures After Plasmodium Berghei Infection
AU  - Esha Sarkar
AU  - Anchal Trivedi
AU  - Aparna Misra
AU  - Anil Kumar Balapure
Y1  - 2022/05/10
PY  - 2022
N1  - https://doi.org/10.11648/j.pst.20220601.12
DO  - 10.11648/j.pst.20220601.12
T2  - Pharmaceutical Science and Technology
JF  - Pharmaceutical Science and Technology
JO  - Pharmaceutical Science and Technology
SP  - 6
EP  - 25
PB  - Science Publishing Group
SN  - 2640-4540
UR  - https://doi.org/10.11648/j.pst.20220601.12
AB  - Background: Malaria is the major cause of disease and death on a global basis. The situation has worsened due to the emergence of parasite strains, resistant to a number of known antimalarials, causing relapse mostly in case of P vivax. That’s why the great need of combination drug therapy along with known compounds and phytocompound came into the picture for dealing drug resistance by introducing a new therapeutic approach. As liver and spleen are found to play the major roles to overcome the complications during malarial parasitemia, we have focused on the alteration of DNA synthesis and protein content during different levels of parasitemia before and after drug application. The study aims to analyse of the activity of Chloroquine and Picroliv on DNA content and protein turn over in both liver and spleen explants culture after P. berghei infection. Histological preparation of spleen explants for paraplast embedding total DNA content and protein turnover. In liver: In malarial, DNA content is observed to decline rapidly as the parasitemia level increases in liver tissue culture. Decrement of DNA content was found almost 25-52% as the parasitemia level increases 60% up to 0-4 days of culture. After insulin supplementation DNA content was found to increase almost 23% on Day 2 and 33% on day 4. Addition of picroliv enhances DNA content about 32%, 11% on day 2 and 4 respectively. Effect of Chloroquine is also observed with insulin and picroliv administration. Throughout the process, total protein concentration was observed to get decreased in proportion to the rate of infection. At 23% of infection protein values decreased by about 4 of culture respectively over the normal controls. Rate of 3-H-Thymidine incorporation is also observed at different stages of parasitemia. Spleen: In spleen also the same tests were done to observe DNA content at different parasitemia levels, where the reduction of DNA content was observed at 60% infection to be as 11% on day3. After addition of insulin it was increased by 133%; infections. Further increase was also observed about 94%. Total protein concentration was also observed to decrease by 23%, 66% at 20% parasitaemia on day 0 and 3. Total DNA content and total protein content was observed to decrease as the parasitemia level increases in cases of liver and spleen. After addition of insulin, could increase the DNA content slightly, further enhanced by application of picroliv in the media.
VL  - 6
IS  - 1
ER  - 

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