Conventional medications used to treat ulcers are not easily accessible to remote areas, costly and not without side effects, thus causing many patients residing in rural areas to resort to herbal means of treatment. World Health Organization records that at least 80% of the world’s population depends on herbal medicinal products. Herbal therapy is the belief that it will promote healthier living. Azadirachta indica is a tree extensively spread in the Northern parts and sparsely distributed in the Northwest Region of Cameroon, used as a remedy for several pathologies, amongst which we have gastric ulcers which is our area of interest. The objective of this study was to evaluate the acute oral toxicity (420 OECD guidelines), systemic exposure and biochemical parameters of toxicity of Azadirachta indica. A. Juss on Wistar rats. Various biochemical parameters such as the: MDA, Catalase, Glutathione, Pepsin, SOD, ASAT, ALAT, Creatinine, XO, and total proteins, were quantified. The acute oral toxicity was of 2000 mg/Kg single dose and compared to a control group which was administered tap water. The administration of 2000 mg/Kg was well tolerated and no death was recorded throughout the fourteen days of observation. No toxic effects were recorded in the organs, implying that at the dose of 2000 mg/Kg, Azadirachta indica was safe. Azadirachta indica aqueous leaf extract contains active metabolites coumarins, catechic tannins, polyphenols, tannins, flavonoids and phlobotannins that were bioavailable in systemic circulation. Showed bioavailability at the tested doses (12.5, 25 and 50 mg/Kg), with the presence of phytochemicals being dose dependent. A clean toxicity profile, with just a slight increase in the level of creatinine.
| Published in | Journal of Drug Design and Medicinal Chemistry (Volume 5, Issue 1) |
| DOI | 10.11648/j.jddmc.20190501.12 |
| Page(s) | 10-17 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Azdiracthta Indica, Systemic Exposure, Toxicity Bioactive Compounds Wistar Rats
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APA Style
Tembe Fokunang Estella, Ndi Sirri Akwen, Njinkio Borgia Nono, Tsabang Nole, Ndikum Valentin Nchafor, et al. (2019). Investigation of Toxicity and Systemic Exposure of Bioactive Compounds of Aqueous Leaf Extract of Azadirachta indica A. Juss (Meliaceae) on Wistar Rats. Journal of Drug Design and Medicinal Chemistry, 5(1), 10-17. https://doi.org/10.11648/j.jddmc.20190501.12
ACS Style
Tembe Fokunang Estella; Ndi Sirri Akwen; Njinkio Borgia Nono; Tsabang Nole; Ndikum Valentin Nchafor, et al. Investigation of Toxicity and Systemic Exposure of Bioactive Compounds of Aqueous Leaf Extract of Azadirachta indica A. Juss (Meliaceae) on Wistar Rats. J. Drug Des. Med. Chem. 2019, 5(1), 10-17. doi: 10.11648/j.jddmc.20190501.12
AMA Style
Tembe Fokunang Estella, Ndi Sirri Akwen, Njinkio Borgia Nono, Tsabang Nole, Ndikum Valentin Nchafor, et al. Investigation of Toxicity and Systemic Exposure of Bioactive Compounds of Aqueous Leaf Extract of Azadirachta indica A. Juss (Meliaceae) on Wistar Rats. J Drug Des Med Chem. 2019;5(1):10-17. doi: 10.11648/j.jddmc.20190501.12
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Download@article{10.11648/j.jddmc.20190501.12,
author = {Tembe Fokunang Estella and Ndi Sirri Akwen and Njinkio Borgia Nono and Tsabang Nole and Ndikum Valentin Nchafor and Ngameni Bathelemy and Nguefack Tsague Georges and Charles Fokunang and Ngadjui Bonaventure Tchaleu},
title = {Investigation of Toxicity and Systemic Exposure of Bioactive Compounds of Aqueous Leaf Extract of Azadirachta indica A. Juss (Meliaceae) on Wistar Rats},
journal = {Journal of Drug Design and Medicinal Chemistry},
volume = {5},
number = {1},
pages = {10-17},
doi = {10.11648/j.jddmc.20190501.12},
url = {https://doi.org/10.11648/j.jddmc.20190501.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jddmc.20190501.12},
abstract = {Conventional medications used to treat ulcers are not easily accessible to remote areas, costly and not without side effects, thus causing many patients residing in rural areas to resort to herbal means of treatment. World Health Organization records that at least 80% of the world’s population depends on herbal medicinal products. Herbal therapy is the belief that it will promote healthier living. Azadirachta indica is a tree extensively spread in the Northern parts and sparsely distributed in the Northwest Region of Cameroon, used as a remedy for several pathologies, amongst which we have gastric ulcers which is our area of interest. The objective of this study was to evaluate the acute oral toxicity (420 OECD guidelines), systemic exposure and biochemical parameters of toxicity of Azadirachta indica. A. Juss on Wistar rats. Various biochemical parameters such as the: MDA, Catalase, Glutathione, Pepsin, SOD, ASAT, ALAT, Creatinine, XO, and total proteins, were quantified. The acute oral toxicity was of 2000 mg/Kg single dose and compared to a control group which was administered tap water. The administration of 2000 mg/Kg was well tolerated and no death was recorded throughout the fourteen days of observation. No toxic effects were recorded in the organs, implying that at the dose of 2000 mg/Kg, Azadirachta indica was safe. Azadirachta indica aqueous leaf extract contains active metabolites coumarins, catechic tannins, polyphenols, tannins, flavonoids and phlobotannins that were bioavailable in systemic circulation. Showed bioavailability at the tested doses (12.5, 25 and 50 mg/Kg), with the presence of phytochemicals being dose dependent. A clean toxicity profile, with just a slight increase in the level of creatinine.},
year = {2019}
}
TY - JOUR T1 - Investigation of Toxicity and Systemic Exposure of Bioactive Compounds of Aqueous Leaf Extract of Azadirachta indica A. Juss (Meliaceae) on Wistar Rats AU - Tembe Fokunang Estella AU - Ndi Sirri Akwen AU - Njinkio Borgia Nono AU - Tsabang Nole AU - Ndikum Valentin Nchafor AU - Ngameni Bathelemy AU - Nguefack Tsague Georges AU - Charles Fokunang AU - Ngadjui Bonaventure Tchaleu Y1 - 2019/06/24 PY - 2019 N1 - https://doi.org/10.11648/j.jddmc.20190501.12 DO - 10.11648/j.jddmc.20190501.12 T2 - Journal of Drug Design and Medicinal Chemistry JF - Journal of Drug Design and Medicinal Chemistry JO - Journal of Drug Design and Medicinal Chemistry SP - 10 EP - 17 PB - Science Publishing Group SN - 2472-3576 UR - https://doi.org/10.11648/j.jddmc.20190501.12 AB - Conventional medications used to treat ulcers are not easily accessible to remote areas, costly and not without side effects, thus causing many patients residing in rural areas to resort to herbal means of treatment. World Health Organization records that at least 80% of the world’s population depends on herbal medicinal products. Herbal therapy is the belief that it will promote healthier living. Azadirachta indica is a tree extensively spread in the Northern parts and sparsely distributed in the Northwest Region of Cameroon, used as a remedy for several pathologies, amongst which we have gastric ulcers which is our area of interest. The objective of this study was to evaluate the acute oral toxicity (420 OECD guidelines), systemic exposure and biochemical parameters of toxicity of Azadirachta indica. A. Juss on Wistar rats. Various biochemical parameters such as the: MDA, Catalase, Glutathione, Pepsin, SOD, ASAT, ALAT, Creatinine, XO, and total proteins, were quantified. The acute oral toxicity was of 2000 mg/Kg single dose and compared to a control group which was administered tap water. The administration of 2000 mg/Kg was well tolerated and no death was recorded throughout the fourteen days of observation. No toxic effects were recorded in the organs, implying that at the dose of 2000 mg/Kg, Azadirachta indica was safe. Azadirachta indica aqueous leaf extract contains active metabolites coumarins, catechic tannins, polyphenols, tannins, flavonoids and phlobotannins that were bioavailable in systemic circulation. Showed bioavailability at the tested doses (12.5, 25 and 50 mg/Kg), with the presence of phytochemicals being dose dependent. A clean toxicity profile, with just a slight increase in the level of creatinine. VL - 5 IS - 1 ER -
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