Effect of Lead Graded Doses in <i>Mactra Corallina</i> Gills: Antioxidants Status, Cholinergic Function and Histopathological Studies

Imene Chetoui; Safa Bejaoui; Chaima Fouzai; Wafa Trabelsi; Salwa Nechi; Emna Chelbi; Mohamed Ghalghaf; M’hamed El Cafsi; Nejla Soudani

doi:doi:10.11648/j.jddmc.20190501.11

Effect of Lead Graded Doses in Mactra Corallina Gills: Antioxidants Status, Cholinergic Function and Histopathological Studies

Imene Chetoui, Safa Bejaoui, Chaima Fouzai, Wafa Trabelsi, Salwa Nechi, Emna Chelbi, Mohamed Ghalghaf, M’hamed El Cafsi, Nejla Soudani

Published in Journal of Drug Design and Medicinal Chemistry (Volume 5, Issue 1)

Received: 12 March 2019 Accepted: 27 April 2019 Published: 12 June 2019

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Lead is non-essential toxic metal used in the industrial process causes severe risk to aquatic organisms. This study aimed (aims) to evaluate the effect of Pb on oxidative stress in gills of Mactra corallina. During the experiment, bivalves were randomly divided into four groups, control served as control and D1, D2 and D3 groups were exposed to Pb graded doses (1mg/L, 2.5mg/L and 5mg/L) during 5 days, respectively. Pb accumulation was significantly increased in all treated gills with doses dependent manner. The exposure of M. corallina to PbCl₂ promoted oxidative stress in gills with an increase in malondialdehyde (MDA) and in metallothionein (MTs) levels. Moreover, a decline in glutathione (GSH), non-protein–SH (NPSH) and ascorbic acid (Vit C) levels were detected in all treated groups. Thus, alterations of enzymatic antioxidants systems were confirmed by a significant increase of catalase (CAT) and decreases of glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities in doses dependent manner. The cholinergic function was confirmed by a significant decrease of acetylcholinesterase (AChE) activity in the highest exposure dose. The impairment of the gill function was confirmed by the histological study.

Published in	Journal of Drug Design and Medicinal Chemistry (Volume 5, Issue 1)
DOI	10.11648/j.jddmc.20190501.11
Page(s)	1-9
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Gills, Histopathological Studies, Lead Exposure, Mactra corallina, Oxidative Stress

References

[1]	J. A. Campillo, M. N. Albentosa, J. Valdes, R. Moreno-Gonzalez, and V. M. Leon, “Impact assessment of agricultural inputs into a Mediterranean coastal lagoon (Mar Menor, SE Spain) on transplanted clams (Ruditapes decussatus) by biochemical and physiological responses, ”Aqua. Toxicol, Vol. 142, pp. 365-379, 2013.
[2]	M. Dellali, M. Romeo, M. Gnassia-Barelli, and P. Aissa, “A multivariate data analysis of the clam Ruditapes decussatus as sentinel organism of the Bizert a lagoon (Tunisia),” Water. Air. Soil. Pollut, Vol. 156, pp. 131-144, 2004.
[3]	S. Mombo, C. Dumat, M. Shahid, and et al, “Socio-scientific analysis of the environmental and health benefits as well as potential risks of cassava production and consumption,” Environ. Sci. Pollut. R, Vol. 24, pp. 5207-5221, 2016.
[4]	P. Shenai Tirodkar, MU. Gauns, MWA, Mujawar, and et al, “Antioxidant responses in gills and digestive gland of oyster Crassostrea madrasensis (Preston) under lead exposure.,” Ecotoxicol. Environ. Saf, Vol. 142, pp. 87-94, 2017.
[5]	Y. De Lafontaine, F. Gagné, C. Blaise, G. Costan, P. Gagnon, and H. M. Chan, “Biomarkers in zebra mussels (Dreissena polymorpha) for the assessment and monitoring of water quality of the St Lawrence River (Canada),” Aqua. Toxicol, Vol. 50, pp. 51-71, 2000.
[6]	MH. S. Kraak, Y. A. Wink, C. S. Stuijfzand, M. C. Buckert-deJong, C. J. de Groot, and W. Admiraal “Chronic ecotoxicity of Zn and Pb to the zebra mussel Dreissena polymorpha, ”Aqua. Toxicol, Vol. 30, pp. 77-89, 1994.
[7]	D. Rittschof, and P. McClellan-Green, “Molluscs as multi disciplinary models in environment toxicology, "Mar. Pollut. Bull, Vol. 50, pp. 369-373, 2005.
[8]	B. Halliwell, and J. M. C. Gutteridge, “Detection of free radicals and other reactive species: trapping and fingerprinting,” In: Free. Radic. Biol. Med, B. Halliwell, and J. M. C. Gutteridge, Eds. Oxford: University Press, 2001, pp. 351-425.
[9]	H. Gurer, and N. Ercal, “Can antioxidants be beneficial in the treatment of lead poisoning?” Free. Radic. Biol. Med, Vol. 29 (10), pp. 927-945, 2000.
[10]	N. Ercal, H. Gurer-Orhan, and N. Aykin-Burns, “Toxic metals and oxidative stress part I: mechanisms involved in metal induced oxidative damage, ”Curr. Top. Med. Chem, Vol. 1 (6), pp. 529–539, 2001.
[11]	P. C. H su, and Y. Guo, “Antioxidant nutrients and lead toxicity,” Toxicol, Vol. 180, pp. 33-44, 2002.
[12]	G. Flora, D. Gupta, and A. Tiwari, “Toxicity of lead: are view with recent up dates,” Interdiscip. Toxicol, Vol. 5 (2), pp. 47–58, 2012.
[13]	O. Chalkiadaki, M. Dassenakis, N. Lydakis–Siùantiris, and M. Scoullos, “Tissue specific, time and dose dependence impact of Lead to a commercial marine Mediterranean organism”, [Lesvos Island. Greece: 11^th Panhellenic Symposium on Oceanography and Fisheries, Mytilene, 2015].
[14]	J. Thyrring, B. K. Juhl, M. Holmstrup, M. E. Blicher, and K. M. Sejr, “Does acute lead (Pb) contamination influence membrane fatty acid composition and freeze tolerance in intertidal blue mussels inarctic Greenland?” Ecotoxicol, 2015, DOI: 10.1007/s10646-015-1539-0.
[15]	Y. H. Cheung, and M. H. Wong, “Trace metal contents of the pacific oyster (Crassostrea gigas) purchased from market sin Hong Kong,” J. Environ. Manag, Vol. 16 (6), 753–761, 1992.
[16]	O. H. Lowry, NJ. Rosebrough, A. L. Farr, and RJ. Randall,“ Protein measurement with the folin phenol reagent.),”J. Exp. Mar. Biol. Ecol, Vol. 12, pp. 103-118, 1951
[17]	H. H. Draper, and M. Hadley, “Malondialdehyde determination as index of lipid 625 peroxidation, ”Method. Enzymol, Vol. 86, pp. 421-431, 1990.
[18]	G. L. Ellman,“Tissue sulfhydryl groups,” Arch. Biochem. Biophysics, Vol. 82, pp. 70-77, 1959.
[19]	M. C. Jacques-Silva, C. W. Nogueira, and L. C. Broch,“ Diphenyldiselenide and ascorbic acid changes deposition of selenium and ascorbic acid in live rand brain of mice,” Pharmacol. Toxicol, Vol. 88, pp. 119-125, 2001.
[20]	C. Beauchamp, and I. Fridovich,”Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels,”Anal. Biochem, Vol. 44, pp. 276-287, 1971.
[21]	L. Flohe, and W. A. Gunzler, “Assays of gluthathione peroxidase,” Method. Enzymol, Vol. 105, pp. 114-121, 1984
[22]	H. Aebi, “Catalase in vitro,” Method. Enzymol, Vol. 105, pp. 121–126, 1984.
[23]	A. Viarengo, E. Ponzano, F. Dondero, and R. Fabbri,“A simple spectrophotometric method for metallothionein evaluation in marine organisms: An application to Mediterranean and Antarctic molluscs,” Mar. Environ. Res, Vol. 44, pp. 69-84, 1997.
[24]	S. Petrovic, B. Ozretic, M. Krajnovic-Ozretic, and D. Bobinac,“ Lysosomal membrane stability and metallothioneins in digestive gland of mussels (Mytilus galloprovincialis Lam.) as biomarkers in a field study,” Mar. Pollut. Bullet, Vol. 42, pp. 1373-1378, 2001.
[25]	G. L. Ellman, K. D. Courtney, V. Abdres, and R. M. Featherstonem, Biochem. Pharmacol. Physiol. 3884-3890. 1961.
[26]	R. Martoja, and M. Martoja-Pierson, Initiation aux techniques de l’histologie animale, Masson et Cieeds, Paris, 1967, pp. 1232.
[27]	B. Fernandez, J. A. Campillo, C. Martinez-Gómez, and J. Benedicto, “Antioxidant responses in gills of mussel (Mytilus galloprovincialis) as biomarkers of environmental stress along the Spanish Mediterranean coast, ”Aquat. Toxicol, Vol. 99, pp. 186–197, 2010.
[28]	A. Viarengo, L. Cansi, M. Pertica, G. Pou, M. N. Moore, and M. Omjneu, “ Heavy metal effects on lipid peroxidation in the tissue of Mytilus galloprovincialis clam,” Comp. Biochem. Physiol, Vol. 97 (C), pp. 37-42, 1990.
[29]	H. Yin, L. Xu, and N. A. Porter,“ Free Radical Lipid Peroxidation. Mechanisms and Analysis,” Chemrev, Vol. 111 (10), pp. 5944-5972, 2011.
[30]	S. Gaweł, M. Wardas, E. Niedworok, and P. Wardas,“ Malondialdehyde (MDA) as a lipid peroxidation marker,”Wiad. Lek, Vol. 57 (9-10), pp. 453-5, 2004.
[31]	S. Bejaoui, K. Telahigue, I. Chetoui, I. Rabeh, C. Fouzai, W. Trabelsi, I. Houas-Gharsallah, M. ElCafsi, and N. Soudani, “Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons,” J. E. C. E, Vol. 2, No. 2, pp. 44-51, 2018.
[32]	S. García-Medina, M. Galar-Martínez, L. M. Gómez-Oliván, K. Ruiz-Lara, H. Islas-Flores, and E. Gasca-Péreza,“Relation ship between genotoxicity and oxidative stress induced by mercury on common carp (Cyprinus carpio) tissues,” Aqua. Toxicol, Vol, 192, pp. 207–215, 2017.
[33]	A. A. Dayem, M. K. Hossain, S. B. Lee, K. Kim, S. K. Saha, G. M Yang, H. Y. Choi, and S. G. Cho,“ The Role of Reactive Oxygen Species (ROS) in the Biological Activities of Metallic Nanoparticles,”Int. J. Mol. Sci, Vol. 18 (1), pp. 120, 2017.
[34]	G. Hariharan, R. Purvaja, and R. Ramesh, “Toxic Effects of lead on biochemical and histological alterations in green mussel (Pernaviridis) induced by environmentally relevant concentrations,” J. Toxicol. Environ. Health, Vol, 77 (A), pp. 246-260, 2014.
[35]	N. Sharma, N. K. Singh, O. P. Singh, V. P and Eyand P. K. Verma, “Oxidative Stress and Antioxidant Status during Transition Period in Dairy Cows,” Asian-Aust. J. Anim. Sci, Vol. 24, No. 4, pp. 479–484, 2011.
[36]	J. P. Coelho, M. Rosa, E. Pereira, A. Duarte, and M. A. Pardal, “Pattern and annual rates of Scrobicularia plana mercury bioaccumulation in a human induced mercury gradient (RiadeAveiro, Portugal),” Estuar. Coast. Shelf. Sci, Vol. 69, pp. 629-635, 2006.
[37]	K. Chaudhary, S. Agarwal, and S. Khan, Role of Phytochelatins (PCs), Metallothioneins (MTs), and Heavy Metal ATPase (HMA) Genesin Heavy Metal Tolerance, Springer International Publishing AG, part of Springer Nature Prasaded, Vol. 39R, Mycoremediation and Environmental Sustainabil, 2018.
[38]	Y, Fang, H. Yangand B. Liu, “Tissue-specific response of metallothionein and superoxide dismutase in the clam Mactra veneriformis under sublethal mercury exposure,”Ecotoxicol, Vol. 21, pp. 1593-1602, 2012.
[39]	L. Guilhermino, P. Barros, M. C. Silva, and A. M. V. M. Soares, “Should the use of inhibition of cholinesterase as a specific biomarker for organophosphate and carbamate pesticides be questioned?” Biomarkers, Vol. 3, pp. 157-163, 1998.
[40]	S. Najimi, A. Bouhaimi, M. Daubèze, A., Zekhnini, J. Pellerin, and J. F. Narbonne, “Use of acetylcholinesterase in Perna perna and Mytilus galloprovincialis as a biomarker of pollution in Agadir Marine Bay (South of Morocco),”Bull. Environ. Contam. Toxicol, Vol. 58 (6), pp. 901-908, 1997.
[41]	A. L. Dafre, I. D. Medeiros, I. C. Muller, E. C. Ventura, and A. C. D. Bainy, “Antioxidant enzymes and thiol /disulfide status in the digestive gland of the brown mussel Perna perna exposed to lead and paraquat,” Chem. Biol. Interact, Vol. 149, pp. 97-105, 2004.

Cite This Article

Plain Text BibTeX RIS

APA Style

Imene Chetoui, Safa Bejaoui, Chaima Fouzai, Wafa Trabelsi, Salwa Nechi, et al. (2019). Effect of Lead Graded Doses in Mactra Corallina Gills: Antioxidants Status, Cholinergic Function and Histopathological Studies. Journal of Drug Design and Medicinal Chemistry, 5(1), 1-9. https://doi.org/10.11648/j.jddmc.20190501.11

Copy | Download

ACS Style

Imene Chetoui; Safa Bejaoui; Chaima Fouzai; Wafa Trabelsi; Salwa Nechi, et al. Effect of Lead Graded Doses in Mactra Corallina Gills: Antioxidants Status, Cholinergic Function and Histopathological Studies. J. Drug Des. Med. Chem. 2019, 5(1), 1-9. doi: 10.11648/j.jddmc.20190501.11

Copy | Download

AMA Style

Imene Chetoui, Safa Bejaoui, Chaima Fouzai, Wafa Trabelsi, Salwa Nechi, et al. Effect of Lead Graded Doses in Mactra Corallina Gills: Antioxidants Status, Cholinergic Function and Histopathological Studies. J Drug Des Med Chem. 2019;5(1):1-9. doi: 10.11648/j.jddmc.20190501.11

Copy | Download

@article{10.11648/j.jddmc.20190501.11,
  author = {Imene Chetoui and Safa Bejaoui and Chaima Fouzai and Wafa Trabelsi and Salwa Nechi and Emna Chelbi and Mohamed Ghalghaf and M’hamed El Cafsi and Nejla Soudani},
  title = {Effect of Lead Graded Doses in Mactra Corallina Gills: Antioxidants Status, Cholinergic Function and Histopathological Studies},
  journal = {Journal of Drug Design and Medicinal Chemistry},
  volume = {5},
  number = {1},
  pages = {1-9},
  doi = {10.11648/j.jddmc.20190501.11},
  url = {https://doi.org/10.11648/j.jddmc.20190501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jddmc.20190501.11},
  abstract = {Lead is non-essential toxic metal used in the industrial process causes severe risk to aquatic organisms. This study aimed (aims) to evaluate the effect of Pb on oxidative stress in gills of Mactra corallina. During the experiment, bivalves were randomly divided into four groups, control served as control and D1, D2 and D3 groups were exposed to Pb graded doses (1mg/L, 2.5mg/L and 5mg/L) during 5 days, respectively. Pb accumulation was significantly increased in all treated gills with doses dependent manner. The exposure of M. corallina to PbCl2 promoted oxidative stress in gills with an increase in malondialdehyde (MDA) and in metallothionein (MTs) levels. Moreover, a decline in glutathione (GSH), non-protein–SH (NPSH) and ascorbic acid (Vit C) levels were detected in all treated groups. Thus, alterations of enzymatic antioxidants systems were confirmed by a significant increase of catalase (CAT) and decreases of glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities in doses dependent manner. The cholinergic function was confirmed by a significant decrease of acetylcholinesterase (AChE) activity in the highest exposure dose. The impairment of the gill function was confirmed by the histological study.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Effect of Lead Graded Doses in Mactra Corallina Gills: Antioxidants Status, Cholinergic Function and Histopathological Studies
AU  - Imene Chetoui
AU  - Safa Bejaoui
AU  - Chaima Fouzai
AU  - Wafa Trabelsi
AU  - Salwa Nechi
AU  - Emna Chelbi
AU  - Mohamed Ghalghaf
AU  - M’hamed El Cafsi
AU  - Nejla Soudani
Y1  - 2019/06/12
PY  - 2019
N1  - https://doi.org/10.11648/j.jddmc.20190501.11
DO  - 10.11648/j.jddmc.20190501.11
T2  - Journal of Drug Design and Medicinal Chemistry
JF  - Journal of Drug Design and Medicinal Chemistry
JO  - Journal of Drug Design and Medicinal Chemistry
SP  - 1
EP  - 9
PB  - Science Publishing Group
SN  - 2472-3576
UR  - https://doi.org/10.11648/j.jddmc.20190501.11
AB  - Lead is non-essential toxic metal used in the industrial process causes severe risk to aquatic organisms. This study aimed (aims) to evaluate the effect of Pb on oxidative stress in gills of Mactra corallina. During the experiment, bivalves were randomly divided into four groups, control served as control and D1, D2 and D3 groups were exposed to Pb graded doses (1mg/L, 2.5mg/L and 5mg/L) during 5 days, respectively. Pb accumulation was significantly increased in all treated gills with doses dependent manner. The exposure of M. corallina to PbCl2 promoted oxidative stress in gills with an increase in malondialdehyde (MDA) and in metallothionein (MTs) levels. Moreover, a decline in glutathione (GSH), non-protein–SH (NPSH) and ascorbic acid (Vit C) levels were detected in all treated groups. Thus, alterations of enzymatic antioxidants systems were confirmed by a significant increase of catalase (CAT) and decreases of glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities in doses dependent manner. The cholinergic function was confirmed by a significant decrease of acetylcholinesterase (AChE) activity in the highest exposure dose. The impairment of the gill function was confirmed by the histological study.
VL  - 5
IS  - 1
ER  -

Copy | Download

Author Information

Imene Chetoui

Faculty of Sciences of Tunis, Biology Department, Laboratory of Ecology, Biology and Physiology of Aquatic Environment, University of Tunis El Manar, Tunis, Tunisia
Safa Bejaoui

Faculty of Sciences of Tunis, Biology Department, Laboratory of Ecology, Biology and Physiology of Aquatic Environment, University of Tunis El Manar, Tunis, Tunisia
Chaima Fouzai

Faculty of Sciences of Tunis, Biology Department, Laboratory of Ecology, Biology and Physiology of Aquatic Environment, University of Tunis El Manar, Tunis, Tunisia
Wafa Trabelsi

Faculty of Sciences of Tunis, Biology Department, Laboratory of Ecology, Biology and Physiology of Aquatic Environment, University of Tunis El Manar, Tunis, Tunisia
Salwa Nechi

Anatomy and Cytology Service, Mohamed Taher Maamouri Hospital, Road Mrezka, Nabeul, Tunisia
Emna Chelbi

Anatomy and Cytology Service, Mohamed Taher Maamouri Hospital, Road Mrezka, Nabeul, Tunisia
Mohamed Ghalghaf

Higher Institute of Fisheries and Aquaculture, Bizerte, Tunisia
M’hamed El Cafsi

Faculty of Sciences of Tunis, Biology Department, Laboratory of Ecology, Biology and Physiology of Aquatic Environment, University of Tunis El Manar, Tunis, Tunisia
Nejla Soudani

Faculty of Sciences of Tunis, Biology Department, Laboratory of Ecology, Biology and Physiology of Aquatic Environment, University of Tunis El Manar, Tunis, Tunisia

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Imene Chetoui, Safa Bejaoui, Chaima Fouzai, Wafa Trabelsi, Salwa Nechi, et al. (2019). Effect of Lead Graded Doses in Mactra Corallina Gills: Antioxidants Status, Cholinergic Function and Histopathological Studies. Journal of Drug Design and Medicinal Chemistry, 5(1), 1-9. https://doi.org/10.11648/j.jddmc.20190501.11

Copy | Download

ACS Style

Imene Chetoui; Safa Bejaoui; Chaima Fouzai; Wafa Trabelsi; Salwa Nechi, et al. Effect of Lead Graded Doses in Mactra Corallina Gills: Antioxidants Status, Cholinergic Function and Histopathological Studies. J. Drug Des. Med. Chem. 2019, 5(1), 1-9. doi: 10.11648/j.jddmc.20190501.11

Copy | Download

AMA Style

Imene Chetoui, Safa Bejaoui, Chaima Fouzai, Wafa Trabelsi, Salwa Nechi, et al. Effect of Lead Graded Doses in Mactra Corallina Gills: Antioxidants Status, Cholinergic Function and Histopathological Studies. J Drug Des Med Chem. 2019;5(1):1-9. doi: 10.11648/j.jddmc.20190501.11

Copy | Download

@article{10.11648/j.jddmc.20190501.11,
  author = {Imene Chetoui and Safa Bejaoui and Chaima Fouzai and Wafa Trabelsi and Salwa Nechi and Emna Chelbi and Mohamed Ghalghaf and M’hamed El Cafsi and Nejla Soudani},
  title = {Effect of Lead Graded Doses in Mactra Corallina Gills: Antioxidants Status, Cholinergic Function and Histopathological Studies},
  journal = {Journal of Drug Design and Medicinal Chemistry},
  volume = {5},
  number = {1},
  pages = {1-9},
  doi = {10.11648/j.jddmc.20190501.11},
  url = {https://doi.org/10.11648/j.jddmc.20190501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jddmc.20190501.11},
  abstract = {Lead is non-essential toxic metal used in the industrial process causes severe risk to aquatic organisms. This study aimed (aims) to evaluate the effect of Pb on oxidative stress in gills of Mactra corallina. During the experiment, bivalves were randomly divided into four groups, control served as control and D1, D2 and D3 groups were exposed to Pb graded doses (1mg/L, 2.5mg/L and 5mg/L) during 5 days, respectively. Pb accumulation was significantly increased in all treated gills with doses dependent manner. The exposure of M. corallina to PbCl2 promoted oxidative stress in gills with an increase in malondialdehyde (MDA) and in metallothionein (MTs) levels. Moreover, a decline in glutathione (GSH), non-protein–SH (NPSH) and ascorbic acid (Vit C) levels were detected in all treated groups. Thus, alterations of enzymatic antioxidants systems were confirmed by a significant increase of catalase (CAT) and decreases of glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities in doses dependent manner. The cholinergic function was confirmed by a significant decrease of acetylcholinesterase (AChE) activity in the highest exposure dose. The impairment of the gill function was confirmed by the histological study.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Effect of Lead Graded Doses in Mactra Corallina Gills: Antioxidants Status, Cholinergic Function and Histopathological Studies
AU  - Imene Chetoui
AU  - Safa Bejaoui
AU  - Chaima Fouzai
AU  - Wafa Trabelsi
AU  - Salwa Nechi
AU  - Emna Chelbi
AU  - Mohamed Ghalghaf
AU  - M’hamed El Cafsi
AU  - Nejla Soudani
Y1  - 2019/06/12
PY  - 2019
N1  - https://doi.org/10.11648/j.jddmc.20190501.11
DO  - 10.11648/j.jddmc.20190501.11
T2  - Journal of Drug Design and Medicinal Chemistry
JF  - Journal of Drug Design and Medicinal Chemistry
JO  - Journal of Drug Design and Medicinal Chemistry
SP  - 1
EP  - 9
PB  - Science Publishing Group
SN  - 2472-3576
UR  - https://doi.org/10.11648/j.jddmc.20190501.11
AB  - Lead is non-essential toxic metal used in the industrial process causes severe risk to aquatic organisms. This study aimed (aims) to evaluate the effect of Pb on oxidative stress in gills of Mactra corallina. During the experiment, bivalves were randomly divided into four groups, control served as control and D1, D2 and D3 groups were exposed to Pb graded doses (1mg/L, 2.5mg/L and 5mg/L) during 5 days, respectively. Pb accumulation was significantly increased in all treated gills with doses dependent manner. The exposure of M. corallina to PbCl2 promoted oxidative stress in gills with an increase in malondialdehyde (MDA) and in metallothionein (MTs) levels. Moreover, a decline in glutathione (GSH), non-protein–SH (NPSH) and ascorbic acid (Vit C) levels were detected in all treated groups. Thus, alterations of enzymatic antioxidants systems were confirmed by a significant increase of catalase (CAT) and decreases of glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities in doses dependent manner. The cholinergic function was confirmed by a significant decrease of acetylcholinesterase (AChE) activity in the highest exposure dose. The impairment of the gill function was confirmed by the histological study.
VL  - 5
IS  - 1
ER  -

Copy | Download