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Evaluation of Acute Dermal Irritation and Wound Contraction by Gymnema Sylvestre and Datura Metel Extracts in Rats

Received: 11 August 2014     Accepted: 21 August 2014     Published: 30 August 2014
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Abstract

In this study, to comparatively evaluate whether aqueous extracts of G. sylvestre and D. metel induce acute dermal irritation and or contract wound tests were performed using experimental animals. The skin of the rats was treated with these extracts (0.5g/dose) and whether the animals sustained skin damage was determined by visual observation. The data demonstrated that the aqueous extracts of both plants did not induce acute skin irritation (toxicity) on the skin of the animals as assessed by pathological observations. Wound healing properties were evaluated using excision wound model. The groups of rats were experimentally wounded at the posterior neck area. An area of uniform wound of 7x7mm using millimetre rule was exercised from the dorsal back of the rats. The animal groups were topically treated with G. sylvestre and D. metel gels. Wound dressed with leaf, stem and flower of G. sylvestre and leaf, stem and fruit of D. metel gel significantly heal earlier than those treated with paraffin base, while the leaf and stem of G. sylvestre and leaf and fruit of D. metel healed wound better than povidone iodine. The results from the present study suggest that these aqueous extracts of G. sylvestre and D. metel have promising potential uses as cosmetic ingredients that do not induce significant levels of dermal irritation and exhibit wound healing properties. Wound contraction elicited by these plant parts in this investigation following topical administration strongly corroborates the verbal claims on their efficacy for these activities.

Published in American Journal of Biomedical and Life Sciences (Volume 2, Issue 4)
DOI 10.11648/j.ajbls.20140204.14
Page(s) 83-88
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Gymnema Sylvestre, Datura Metel, Wound Contraction, Dermal Irritation, Rats

References
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[5] Nayak, B.S., Pinto-Pereira, L.M. (2006). Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats. BMC Complementary and Alternative Medicines. 6: 41-47.
[6] Akinmoladun, A.C., Abukun, E.O; Afor, E., Akinrinlola B.L., Onibon, T.R., Akinboboye, O., Obuotor, E.M. and Farombi, E.O (2007). Chemical constituents and antioxidant activity of Alstonia boonei. African Journal of Biotechnology. 6: 1197-1201.
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[8] Draize, J.H., Woodard, G., Calvery, H.O. (1994). Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes. J. Pharmacol. Exp. Thera. 82: 377-390.
[9] Nigam, P.K. (2009). Adverse reactions to cosmetics and methods of testing. Indian Journal – J. Dermatol Venereol Leprol. 75: 10-18.
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[21] Soliman, Y.,Ibrahim, H., Alqasoumi, S. (2011). Anti-inflammatory and wound healing activities of herbal gel containing an antioxidant Tamarix aphilla leaf extract. International Journal of Pharmacology. 7(8): 829-835.
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    Omale James, Ajidahun Bidemi Sunday. (2014). Evaluation of Acute Dermal Irritation and Wound Contraction by Gymnema Sylvestre and Datura Metel Extracts in Rats. American Journal of Biomedical and Life Sciences, 2(4), 83-88. https://doi.org/10.11648/j.ajbls.20140204.14

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    ACS Style

    Omale James; Ajidahun Bidemi Sunday. Evaluation of Acute Dermal Irritation and Wound Contraction by Gymnema Sylvestre and Datura Metel Extracts in Rats. Am. J. Biomed. Life Sci. 2014, 2(4), 83-88. doi: 10.11648/j.ajbls.20140204.14

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    AMA Style

    Omale James, Ajidahun Bidemi Sunday. Evaluation of Acute Dermal Irritation and Wound Contraction by Gymnema Sylvestre and Datura Metel Extracts in Rats. Am J Biomed Life Sci. 2014;2(4):83-88. doi: 10.11648/j.ajbls.20140204.14

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  • @article{10.11648/j.ajbls.20140204.14,
      author = {Omale James and Ajidahun Bidemi Sunday},
      title = {Evaluation of Acute Dermal Irritation and Wound Contraction by Gymnema Sylvestre and Datura Metel Extracts in Rats},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {2},
      number = {4},
      pages = {83-88},
      doi = {10.11648/j.ajbls.20140204.14},
      url = {https://doi.org/10.11648/j.ajbls.20140204.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140204.14},
      abstract = {In this study, to comparatively evaluate whether aqueous extracts of G. sylvestre and D. metel induce acute dermal irritation and or contract wound tests were performed using experimental animals. The skin of the rats was treated with these extracts (0.5g/dose) and whether the animals sustained skin damage was determined by visual observation. The data demonstrated that the aqueous extracts of both plants did not induce acute skin irritation (toxicity) on the skin of the animals as assessed by pathological observations. Wound healing properties were evaluated using excision wound model. The groups of rats were experimentally wounded at the posterior neck area. An area of uniform wound of 7x7mm using millimetre rule was exercised from the dorsal back of the rats. The animal groups were topically treated with G. sylvestre and D. metel gels. Wound dressed with leaf, stem and flower of G. sylvestre and leaf, stem and fruit of D. metel gel significantly heal earlier than those treated with paraffin base, while the leaf and stem of G. sylvestre and leaf and fruit of D. metel healed wound better than povidone iodine. The results from the present study suggest that these aqueous extracts of G. sylvestre and D. metel have promising potential uses as cosmetic ingredients that do not induce significant levels of dermal irritation and exhibit wound healing properties. Wound contraction elicited by these plant parts in this investigation following topical administration strongly corroborates the verbal claims on their efficacy for these activities.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Evaluation of Acute Dermal Irritation and Wound Contraction by Gymnema Sylvestre and Datura Metel Extracts in Rats
    AU  - Omale James
    AU  - Ajidahun Bidemi Sunday
    Y1  - 2014/08/30
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajbls.20140204.14
    DO  - 10.11648/j.ajbls.20140204.14
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 83
    EP  - 88
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20140204.14
    AB  - In this study, to comparatively evaluate whether aqueous extracts of G. sylvestre and D. metel induce acute dermal irritation and or contract wound tests were performed using experimental animals. The skin of the rats was treated with these extracts (0.5g/dose) and whether the animals sustained skin damage was determined by visual observation. The data demonstrated that the aqueous extracts of both plants did not induce acute skin irritation (toxicity) on the skin of the animals as assessed by pathological observations. Wound healing properties were evaluated using excision wound model. The groups of rats were experimentally wounded at the posterior neck area. An area of uniform wound of 7x7mm using millimetre rule was exercised from the dorsal back of the rats. The animal groups were topically treated with G. sylvestre and D. metel gels. Wound dressed with leaf, stem and flower of G. sylvestre and leaf, stem and fruit of D. metel gel significantly heal earlier than those treated with paraffin base, while the leaf and stem of G. sylvestre and leaf and fruit of D. metel healed wound better than povidone iodine. The results from the present study suggest that these aqueous extracts of G. sylvestre and D. metel have promising potential uses as cosmetic ingredients that do not induce significant levels of dermal irritation and exhibit wound healing properties. Wound contraction elicited by these plant parts in this investigation following topical administration strongly corroborates the verbal claims on their efficacy for these activities.
    VL  - 2
    IS  - 4
    ER  - 

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Author Information
  • Department of Biochemistry, Kogi State University, Anyigba, Nigeria

  • Department of Biochemistry, Kogi State University, Anyigba, Nigeria

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