@article{10.11648/j.ajbls.20140204.11,
  author = {Seidu Mahmood Abdulai and Adams Abdul Rashid and Gyasi Kwame Richard and Tettey Yao and Nkansah Obenewaa Dinah and Adunyame Lois and Wiredu Kwame Edwin},
  title = {Immunohistochemical Study on lipid Catabolism in Adult Onchocerca Volvulus},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {2},
  number = {4},
  pages = {65-69},
  doi = {10.11648/j.ajbls.20140204.11},
  url = {https://doi.org/10.11648/j.ajbls.20140204.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140204.11},
  abstract = {Onchocerca volvulus is a parasite responsible for the disease, Onchocerciasis otherwise known as river blindness. Current treatment and control strategies are not entirely successful. This means there is a need for further studies to better understand the biology of this worm. Studies on metabolic pathways in the worm can provide valuable information to deepen our understanding of the mechanism of survival of the worm. Lipid catabolism for the provision of energy in this worm still remains to be elucidated. Knowledge of lipids composition and synthesis in this parasite has been mostly drawn from studies on filarial species other than O. volvulus itself. This is owed to the limited availability of parasite material due largely to problems with parasite cultivation and ethical demands on obtaining materials since it has a strict preference for the human host. To explore lipid catabolism in the worm itself, we performed immunohistochemical localisation of three major enzymes (A/B hydrolase, SDH and ME1) involved in lipid metabolism on paraffin processed archival O. volvulus nodules. We observed that up to 58.6% of worms in the paraffin processed nodules had detectable A/B/HD4, 51.6% had SDH and 63.3% had ME 1, most of which were stored in the muscles of the adult worm. These observations suggest that the adult O. volvulus can operate the B-oxidative pathway, The TCA cycle, and undergo anaplerotic transformation of malic acid to pyruvate to maximise energy production from lipid metabolism. This also suggests that O. volvulus has the capacity to catabolise lipids for energy even though it appears to be dependent on carbohydrate metabolism.},
 year = {2014}
}

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TY  - JOUR
T1  - Immunohistochemical Study on lipid Catabolism in Adult Onchocerca Volvulus
AU  - Seidu Mahmood Abdulai
AU  - Adams Abdul Rashid
AU  - Gyasi Kwame Richard
AU  - Tettey Yao
AU  - Nkansah Obenewaa Dinah
AU  - Adunyame Lois
AU  - Wiredu Kwame Edwin
Y1  - 2014/07/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajbls.20140204.11
DO  - 10.11648/j.ajbls.20140204.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 65
EP  - 69
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20140204.11
AB  - Onchocerca volvulus is a parasite responsible for the disease, Onchocerciasis otherwise known as river blindness. Current treatment and control strategies are not entirely successful. This means there is a need for further studies to better understand the biology of this worm. Studies on metabolic pathways in the worm can provide valuable information to deepen our understanding of the mechanism of survival of the worm. Lipid catabolism for the provision of energy in this worm still remains to be elucidated. Knowledge of lipids composition and synthesis in this parasite has been mostly drawn from studies on filarial species other than O. volvulus itself. This is owed to the limited availability of parasite material due largely to problems with parasite cultivation and ethical demands on obtaining materials since it has a strict preference for the human host. To explore lipid catabolism in the worm itself, we performed immunohistochemical localisation of three major enzymes (A/B hydrolase, SDH and ME1) involved in lipid metabolism on paraffin processed archival O. volvulus nodules. We observed that up to 58.6% of worms in the paraffin processed nodules had detectable A/B/HD4, 51.6% had SDH and 63.3% had ME 1, most of which were stored in the muscles of the adult worm. These observations suggest that the adult O. volvulus can operate the B-oxidative pathway, The TCA cycle, and undergo anaplerotic transformation of malic acid to pyruvate to maximise energy production from lipid metabolism. This also suggests that O. volvulus has the capacity to catabolise lipids for energy even though it appears to be dependent on carbohydrate metabolism.
VL  - 2
IS  - 4
ER  - 

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