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Biological activities of collagen peptides obtained by enzymic Hydrolysis from Far-Eastern holothurians

Received: 30 May 2013     Published: 30 June 2013
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Abstract

The certain sea cucumbers are the marine animals which are important as human delicacy food source. In addition a number of unique biological and pharmacological active compounds have been ascribed to various species of sea cucumbers. In current work the collagen peptides (CPs) from the bodies of the far-eastern holothurians (sea cucumber) Apostichopus japonicus and Cucumaria japonica by treated with a complex of proteolytic enzymes from Kamchatka crab Paralithodes camtschaticus were obtained, and the antitumor, anticoagulant, anti-inflammatory, and wound healing properties these CPs were estimated. The element and amino acid analysis of CPs from trepang and cucumaria are suggested that it’s could be regarded as a typical collagen fragments containing 6% sulfated carbohydrate and/or amino acid residues. CPs inhibited growth and progression solid Erlich tumor, but not in alike degree. Our results clearly showed, that these CPs possessed the moderate anticoagulant activity, and are preferential inhibitors of the initial link of the blood coagulation system. We also determined that the CPs revealed significant wound healing effect in regarding to thermal wounds and have high anti-inflammatory activity by used carrageenane model of acute inflammatory. Apparently, that the degree of biological activity depend on characteristics of amino acid composition and concentration of CPs. Taken together, these results indicate that CPs from sea cucumbers have similar to biological activities with other collagen peptides (components of the extracellular matrix, endostatin and others) and sulfated polysaccharides. We proposed that the obtained CPs may be applied as components functional food and nontoxic remedies of supplementary therapy for prevention and treatment of various diseases.

Published in American Journal of Biomedical and Life Sciences (Volume 1, Issue 1)
DOI 10.11648/j.ajbls.20130101.14
Page(s) 17-26
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Bioactive Peptides, Collagen Peptides, Sea Cucumber, Marine Drugs, Functional Food, Biological Activity

References
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    Popov A., Popov A., Artyukov A., Krivoshapko O., Kozlovskaya E. (2013). Biological activities of collagen peptides obtained by enzymic Hydrolysis from Far-Eastern holothurians. American Journal of Biomedical and Life Sciences, 1(1), 17-26. https://doi.org/10.11648/j.ajbls.20130101.14

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    ACS Style

    Popov A.; Popov A.; Artyukov A.; Krivoshapko O.; Kozlovskaya E. Biological activities of collagen peptides obtained by enzymic Hydrolysis from Far-Eastern holothurians. Am. J. Biomed. Life Sci. 2013, 1(1), 17-26. doi: 10.11648/j.ajbls.20130101.14

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    AMA Style

    Popov A., Popov A., Artyukov A., Krivoshapko O., Kozlovskaya E. Biological activities of collagen peptides obtained by enzymic Hydrolysis from Far-Eastern holothurians. Am J Biomed Life Sci. 2013;1(1):17-26. doi: 10.11648/j.ajbls.20130101.14

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  • @article{10.11648/j.ajbls.20130101.14,
      author = {Popov A. and Popov A. and Artyukov A. and Krivoshapko O. and Kozlovskaya E.},
      title = {Biological activities of collagen peptides obtained by enzymic Hydrolysis from Far-Eastern holothurians},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {1},
      number = {1},
      pages = {17-26},
      doi = {10.11648/j.ajbls.20130101.14},
      url = {https://doi.org/10.11648/j.ajbls.20130101.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20130101.14},
      abstract = {The certain sea cucumbers are the marine animals which are important as human delicacy food source. In addition a number of unique biological and pharmacological active compounds have been ascribed to various species of sea cucumbers. In current work the collagen peptides (CPs) from the bodies of the far-eastern holothurians (sea cucumber) Apostichopus japonicus and Cucumaria japonica by treated with a complex of proteolytic enzymes from Kamchatka crab Paralithodes camtschaticus were obtained, and the antitumor, anticoagulant, anti-inflammatory, and wound healing properties these CPs were estimated. The element and amino acid analysis of CPs from trepang and cucumaria are suggested that it’s could be regarded as a typical collagen fragments containing 6% sulfated carbohydrate and/or amino acid residues. CPs inhibited growth and progression solid Erlich tumor, but not in alike degree. Our results clearly showed, that these CPs possessed the moderate anticoagulant activity, and are preferential inhibitors of the initial link of the blood coagulation system. We also determined that the CPs revealed significant wound healing effect in regarding to thermal wounds and have high anti-inflammatory activity by used carrageenane model of acute inflammatory. Apparently, that the degree of biological activity depend on characteristics of amino acid composition and concentration of CPs. Taken together, these results indicate that CPs from sea cucumbers have similar to biological activities with other collagen peptides (components of the extracellular matrix, endostatin and others) and sulfated polysaccharides. We proposed that the obtained CPs may be applied as components functional food and nontoxic remedies of supplementary therapy for prevention and treatment of various diseases.},
     year = {2013}
    }
    

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  • TY  - JOUR
    T1  - Biological activities of collagen peptides obtained by enzymic Hydrolysis from Far-Eastern holothurians
    AU  - Popov A.
    AU  - Popov A.
    AU  - Artyukov A.
    AU  - Krivoshapko O.
    AU  - Kozlovskaya E.
    Y1  - 2013/06/30
    PY  - 2013
    N1  - https://doi.org/10.11648/j.ajbls.20130101.14
    DO  - 10.11648/j.ajbls.20130101.14
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 17
    EP  - 26
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20130101.14
    AB  - The certain sea cucumbers are the marine animals which are important as human delicacy food source. In addition a number of unique biological and pharmacological active compounds have been ascribed to various species of sea cucumbers. In current work the collagen peptides (CPs) from the bodies of the far-eastern holothurians (sea cucumber) Apostichopus japonicus and Cucumaria japonica by treated with a complex of proteolytic enzymes from Kamchatka crab Paralithodes camtschaticus were obtained, and the antitumor, anticoagulant, anti-inflammatory, and wound healing properties these CPs were estimated. The element and amino acid analysis of CPs from trepang and cucumaria are suggested that it’s could be regarded as a typical collagen fragments containing 6% sulfated carbohydrate and/or amino acid residues. CPs inhibited growth and progression solid Erlich tumor, but not in alike degree. Our results clearly showed, that these CPs possessed the moderate anticoagulant activity, and are preferential inhibitors of the initial link of the blood coagulation system. We also determined that the CPs revealed significant wound healing effect in regarding to thermal wounds and have high anti-inflammatory activity by used carrageenane model of acute inflammatory. Apparently, that the degree of biological activity depend on characteristics of amino acid composition and concentration of CPs. Taken together, these results indicate that CPs from sea cucumbers have similar to biological activities with other collagen peptides (components of the extracellular matrix, endostatin and others) and sulfated polysaccharides. We proposed that the obtained CPs may be applied as components functional food and nontoxic remedies of supplementary therapy for prevention and treatment of various diseases.
    VL  - 1
    IS  - 1
    ER  - 

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Author Information
  • G. B. Elyakov Pacific Institute of Bioorganic Chemistry Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia

  • G. B. Elyakov Pacific Institute of Bioorganic Chemistry Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia

  • G. B. Elyakov Pacific Institute of Bioorganic Chemistry Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia

  • G. B. Elyakov Pacific Institute of Bioorganic Chemistry Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia

  • G. B. Elyakov Pacific Institute of Bioorganic Chemistry Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia

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