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Binding of Heavy Metals with C-Phycocyanin: A Comparison between Equilibrium Dialysis, Fluorescence and Absorption Titration

Received: 7 May 2013     Published: 10 June 2013
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Abstract

The energetic of interaction of metal ions with C-Phycocyanin (C-PC) Spirulina platensis were investigated using optical and thermodynamic methods. Comparison of the binding constants of heavy metals with C-PC by the instrumentality of difference methods shown, that binding affinities of toxic and heavy metal ions for C-PC strongly depend upon the metal. The efficiency of interaction for different metals is arranged in the same sequence for the absorption titration as it is for fluorescence. Obtained results using equilibrium dialysis are in good agreement with results using fluorescence spectroscopy in the case Ag+, but difference is observed for Ni2+ and Cr3+ ions.

Published in American Journal of Biomedical and Life Sciences (Volume 1, Issue 1)
DOI 10.11648/j.ajbls.20130101.13
Page(s) 12-16
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

C-Phycocyanin, Heavy Metals, Binding Constants

References
[1] D.Jalajia Kumari, B. Babitha, SK. Jaffar, M.Guru Prasad MD. Ibrahim and MD.Siddque, A. Khan,"Potential health benefits of Spirulina platensis", An International Journal of Advances In Pharmaceutical Sciences 2011, 2(5-6): 417-422.
[2] V. Rimbau, A. Camins, C. Romay, R. Gonzalez, M. Pallas, "Protective effect of C-phycocyanin against kainic acidinduced neuronal damage in rat hippocampus", Neuroscience Letters, 1999, 276:75-78.
[3] S. Ayehunie, A. Belay, TW. Baba, RM.Ruprecht, "Inhibition of HIV-1 replication by an aqueous extract of Spirulina platensis (Arthrospira platensis)", J Acquired Immune Deficiency Syndromes and Human Retrovirology, 1998, 18: 7-12.
[4] M. Muthulakshmi, A. Saranya, M. Sudha, and G. Selvakumar," Extraction, partial purification, and antibacterial activity of phycocyanin from Spirulina isolated from fresh water body against various human pathogens", J. Algal Biomass Utln. 2012, 3 (3): 7– 11.
[5] Nagendra G. Babu, Phaninatha A. Sarma, dress H. Attitalla and S.D.S. Murthy, "Effect of selected heavy metal ions on the photosynthetic electron transport and energy transfer in the thylakoid membrane of the cyanobacterium, Spirulina platensis, Academic Journal of Plant Sciences, 2010, 3 (1): 46-49.
[6] H.A Kupper, B.Parameswaran,M.Leitenmaier, Trtilek and I.Setlik. Cadmium-induced inhibition of photosynthesis and long-term acclimation to cadmium stress in the hyperaccumulator Thlasp caerulescens, New Phytol., 2007, 175: 655-674.
[7] F.W.J. Teale, R.E.Dale, "Isolation and spectral characterization of phycobiliproteins", Biochem.J.1970, 116: 161-169.
[8] H.J. Li, D.M. Crothers,"Relaxation studies of the proflavine-DNA complex: the kinetics of an intercalation reaction", J Mol Biol. 1969, 39(3): 461–477.
[9] A.M. Pyle, J.P. Rehmann, C.V.Kumar, N.J.Turro, J.K.Batton,"Mixed ligand complexes of ruthenium(II): factors governing binding to DNA", J. Am. Chem. Soc. 1989, 111: 3051-3057.
[10] R.X.Tubbs, W.H. Ditmates, Jr. Winkle, and Q. V. ’’Heterogeneity of the interaction of DNA with acriflavine", J.Mol.Biol. 1964, 9: 545 - 557.
[11] G. Scatchard, "The attraction of proteins for small molecules and ions", Ann.N.Y.Acad.Sci. 1949, 51: 660-672.
[12] A.V. Hill, "The possible effects of the aggregation of the molecules of hemoglobin on its dissotiation curves", J.Physiol., 1910, London,40,iv.
[13] C.R. Cantor, and P.R. Schimmel, Biophysical Chemistry. Part III, p. 534, Moscow, Mir, 1985, (in Russian).
[14] Inci Ozer, Ozden Tacal,"Method dependence of apparent stoichiometry in the binding of salicilate ion to human serum albumi: A comparison between equilibrium dialysis and fluorescence titration, Analytical biochemistry, 2001,294(1):1-6.
[15] E. S. Gelagutashvili, A.I. Belokobilsky, A. I .Rcheulishvili, L. M. Mosulishvili, "Interaction of Pb(II) ions with C-phycocyanin: Effect of ionic strength", Biophysics, 2003, 48: 589-594.
[16] E. Gelagutashvili, A. Khizanishvili, E. Ginturi, N. Kuchava, N. Bagdavadze, A.Rcheulishvili and L. Mosulishvili, "Strength of binding for Cd(II) - C- Phycocyanin (from Spirulina platensis) complexes by equilibrium dialysis’’, Journal of Biological Physics and Chemistry, 2007, 7: 103-106.
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  • APA Style

    Eteri Gelagutashvili. (2013). Binding of Heavy Metals with C-Phycocyanin: A Comparison between Equilibrium Dialysis, Fluorescence and Absorption Titration. American Journal of Biomedical and Life Sciences, 1(1), 12-16. https://doi.org/10.11648/j.ajbls.20130101.13

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    ACS Style

    Eteri Gelagutashvili. Binding of Heavy Metals with C-Phycocyanin: A Comparison between Equilibrium Dialysis, Fluorescence and Absorption Titration. Am. J. Biomed. Life Sci. 2013, 1(1), 12-16. doi: 10.11648/j.ajbls.20130101.13

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    AMA Style

    Eteri Gelagutashvili. Binding of Heavy Metals with C-Phycocyanin: A Comparison between Equilibrium Dialysis, Fluorescence and Absorption Titration. Am J Biomed Life Sci. 2013;1(1):12-16. doi: 10.11648/j.ajbls.20130101.13

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  • @article{10.11648/j.ajbls.20130101.13,
      author = {Eteri Gelagutashvili},
      title = {Binding of Heavy Metals with C-Phycocyanin: A Comparison between Equilibrium Dialysis, Fluorescence and Absorption Titration},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {1},
      number = {1},
      pages = {12-16},
      doi = {10.11648/j.ajbls.20130101.13},
      url = {https://doi.org/10.11648/j.ajbls.20130101.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20130101.13},
      abstract = {The energetic of interaction of metal ions with C-Phycocyanin (C-PC) Spirulina platensis were investigated using optical and thermodynamic methods. Comparison of the binding constants of heavy metals with C-PC by the instrumentality of difference methods shown, that binding affinities of toxic and heavy metal ions for C-PC strongly depend upon the metal. The efficiency of interaction for different metals is arranged in the same sequence for the absorption titration as it is for fluorescence. Obtained results using equilibrium dialysis are in good agreement with results using fluorescence spectroscopy in the case Ag+, but difference is observed for Ni2+ and Cr3+ ions.},
     year = {2013}
    }
    

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    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
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    UR  - https://doi.org/10.11648/j.ajbls.20130101.13
    AB  - The energetic of interaction of metal ions with C-Phycocyanin (C-PC) Spirulina platensis were investigated using optical and thermodynamic methods. Comparison of the binding constants of heavy metals with C-PC by the instrumentality of difference methods shown, that binding affinities of toxic and heavy metal ions for C-PC strongly depend upon the metal. The efficiency of interaction for different metals is arranged in the same sequence for the absorption titration as it is for fluorescence. Obtained results using equilibrium dialysis are in good agreement with results using fluorescence spectroscopy in the case Ag+, but difference is observed for Ni2+ and Cr3+ ions.
    VL  - 1
    IS  - 1
    ER  - 

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Author Information
  • Andronikashvili Institute of Physics, Ivane Javakhishvili Tbilisi State University, Tbilisi, Georgia

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