This study investigated the claims of traditional practitioners in the use of Anthocleista djalonensis for the treatment of various diseases and infections in Benue State, Nigeria. The leaves of the plant were collected; air dried; pulverized and successively extracted using hexane, ethyl acetate, ethanol and methanol by microwave assisted method. The phytochemical analysis of the leaf extracts of Anthocleista djalonensis revealed the presence of glycosides, saponins, terpenes, sterols flavonoids, anthraquinones, resins and balsams in Anthocleista djalonensis leaf. The antimicrobial screening of the hexane, ethyl acetate, ethanol and methanol extracts were carried out on Methicillin Resistant Staphylococcus aureus, Vancomycin Resistant enterococci, Staphylococcus aureus, Helicobacter pylori, Candida albicans, Candida krusei, Candida tropicalis, Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa using agar-well diffusion method. The antimicrobial studies showed that all the extracts exhibited activities against Methicillin Resistant Staphylococcus aureus, Vancomycin Resistant enterococci, S. aureus, H. pylori, C. albicans, and C. krusei with significant zones of inhibition ranging from 16 - 20 mm for hexane extract, 22 - 28 mm for ethyl acetate extract, 20 - 24 mm for ethanol extract and 20 - 23 mm for methanol extract against test microbes. E. coli, P. aeruginosa, P. mirabilis and C. tropicalis showed resistance to the extracts; Minimum Inhibitory Concentration (MIC) of the extracts against the stated microbes were 5 mg/mL, 5 mg/mL, 5 mg/mL, 5 mg/mL, 2.5 mg/mL and 5 mg/mL respectively for hexane extract. 1.25 mg/mL, 1.25 mg/mL, 0.62 mg/mL, 0.62 mg/mL 0.62 mg/mL and 1.25 mg/mL respectively for ethyl acetate extract; Ethanol and methanol extracts recorded 1.25 mg/mL against all the stated test microbes. The minimum bactericidal/fungicidal concentration of the extracts against Methicillin Resistant Staphylococcus aureus, Vancomycin Resistant enterococci, S. aureus, H. pylori, C. albicans and C. krusei ranged from 5 mg/mL to 1.25 mg/mL. The results support the use of Anthocleista djalonensis in traditional medicine.
| Published in | Journal of Drug Design and Medicinal Chemistry (Volume 3, Issue 5) |
| DOI | 10.11648/j.jddmc.20170305.12 |
| Page(s) | 71-76 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2017. Published by Science Publishing Group |
Antimicrobial, Phytochemicals, Anthocleista djalonensis and Traditional Medicine
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APA Style
Ijeoma Solomon Okoro, Sampson Dominic Umoh, John Vershima Anyam, Manasseh Msugh-Ter Manyi, Cynthia Adegbe. (2017). In-vitro Antimicrobial Properties and Phytochemical Constituents of Anthocleista djalonensis Leaf Extracts. Journal of Drug Design and Medicinal Chemistry, 3(5), 71-76. https://doi.org/10.11648/j.jddmc.20170305.12
ACS Style
Ijeoma Solomon Okoro; Sampson Dominic Umoh; John Vershima Anyam; Manasseh Msugh-Ter Manyi; Cynthia Adegbe. In-vitro Antimicrobial Properties and Phytochemical Constituents of Anthocleista djalonensis Leaf Extracts. J. Drug Des. Med. Chem. 2017, 3(5), 71-76. doi: 10.11648/j.jddmc.20170305.12
AMA Style
Ijeoma Solomon Okoro, Sampson Dominic Umoh, John Vershima Anyam, Manasseh Msugh-Ter Manyi, Cynthia Adegbe. In-vitro Antimicrobial Properties and Phytochemical Constituents of Anthocleista djalonensis Leaf Extracts. J Drug Des Med Chem. 2017;3(5):71-76. doi: 10.11648/j.jddmc.20170305.12
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author = {Ijeoma Solomon Okoro and Sampson Dominic Umoh and John Vershima Anyam and Manasseh Msugh-Ter Manyi and Cynthia Adegbe},
title = {In-vitro Antimicrobial Properties and Phytochemical Constituents of Anthocleista djalonensis Leaf Extracts},
journal = {Journal of Drug Design and Medicinal Chemistry},
volume = {3},
number = {5},
pages = {71-76},
doi = {10.11648/j.jddmc.20170305.12},
url = {https://doi.org/10.11648/j.jddmc.20170305.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jddmc.20170305.12},
abstract = {This study investigated the claims of traditional practitioners in the use of Anthocleista djalonensis for the treatment of various diseases and infections in Benue State, Nigeria. The leaves of the plant were collected; air dried; pulverized and successively extracted using hexane, ethyl acetate, ethanol and methanol by microwave assisted method. The phytochemical analysis of the leaf extracts of Anthocleista djalonensis revealed the presence of glycosides, saponins, terpenes, sterols flavonoids, anthraquinones, resins and balsams in Anthocleista djalonensis leaf. The antimicrobial screening of the hexane, ethyl acetate, ethanol and methanol extracts were carried out on Methicillin Resistant Staphylococcus aureus, Vancomycin Resistant enterococci, Staphylococcus aureus, Helicobacter pylori, Candida albicans, Candida krusei, Candida tropicalis, Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa using agar-well diffusion method. The antimicrobial studies showed that all the extracts exhibited activities against Methicillin Resistant Staphylococcus aureus, Vancomycin Resistant enterococci, S. aureus, H. pylori, C. albicans, and C. krusei with significant zones of inhibition ranging from 16 - 20 mm for hexane extract, 22 - 28 mm for ethyl acetate extract, 20 - 24 mm for ethanol extract and 20 - 23 mm for methanol extract against test microbes. E. coli, P. aeruginosa, P. mirabilis and C. tropicalis showed resistance to the extracts; Minimum Inhibitory Concentration (MIC) of the extracts against the stated microbes were 5 mg/mL, 5 mg/mL, 5 mg/mL, 5 mg/mL, 2.5 mg/mL and 5 mg/mL respectively for hexane extract. 1.25 mg/mL, 1.25 mg/mL, 0.62 mg/mL, 0.62 mg/mL 0.62 mg/mL and 1.25 mg/mL respectively for ethyl acetate extract; Ethanol and methanol extracts recorded 1.25 mg/mL against all the stated test microbes. The minimum bactericidal/fungicidal concentration of the extracts against Methicillin Resistant Staphylococcus aureus, Vancomycin Resistant enterococci, S. aureus, H. pylori, C. albicans and C. krusei ranged from 5 mg/mL to 1.25 mg/mL. The results support the use of Anthocleista djalonensis in traditional medicine.},
year = {2017}
}
TY - JOUR T1 - In-vitro Antimicrobial Properties and Phytochemical Constituents of Anthocleista djalonensis Leaf Extracts AU - Ijeoma Solomon Okoro AU - Sampson Dominic Umoh AU - John Vershima Anyam AU - Manasseh Msugh-Ter Manyi AU - Cynthia Adegbe Y1 - 2017/11/20 PY - 2017 N1 - https://doi.org/10.11648/j.jddmc.20170305.12 DO - 10.11648/j.jddmc.20170305.12 T2 - Journal of Drug Design and Medicinal Chemistry JF - Journal of Drug Design and Medicinal Chemistry JO - Journal of Drug Design and Medicinal Chemistry SP - 71 EP - 76 PB - Science Publishing Group SN - 2472-3576 UR - https://doi.org/10.11648/j.jddmc.20170305.12 AB - This study investigated the claims of traditional practitioners in the use of Anthocleista djalonensis for the treatment of various diseases and infections in Benue State, Nigeria. The leaves of the plant were collected; air dried; pulverized and successively extracted using hexane, ethyl acetate, ethanol and methanol by microwave assisted method. The phytochemical analysis of the leaf extracts of Anthocleista djalonensis revealed the presence of glycosides, saponins, terpenes, sterols flavonoids, anthraquinones, resins and balsams in Anthocleista djalonensis leaf. The antimicrobial screening of the hexane, ethyl acetate, ethanol and methanol extracts were carried out on Methicillin Resistant Staphylococcus aureus, Vancomycin Resistant enterococci, Staphylococcus aureus, Helicobacter pylori, Candida albicans, Candida krusei, Candida tropicalis, Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa using agar-well diffusion method. The antimicrobial studies showed that all the extracts exhibited activities against Methicillin Resistant Staphylococcus aureus, Vancomycin Resistant enterococci, S. aureus, H. pylori, C. albicans, and C. krusei with significant zones of inhibition ranging from 16 - 20 mm for hexane extract, 22 - 28 mm for ethyl acetate extract, 20 - 24 mm for ethanol extract and 20 - 23 mm for methanol extract against test microbes. E. coli, P. aeruginosa, P. mirabilis and C. tropicalis showed resistance to the extracts; Minimum Inhibitory Concentration (MIC) of the extracts against the stated microbes were 5 mg/mL, 5 mg/mL, 5 mg/mL, 5 mg/mL, 2.5 mg/mL and 5 mg/mL respectively for hexane extract. 1.25 mg/mL, 1.25 mg/mL, 0.62 mg/mL, 0.62 mg/mL 0.62 mg/mL and 1.25 mg/mL respectively for ethyl acetate extract; Ethanol and methanol extracts recorded 1.25 mg/mL against all the stated test microbes. The minimum bactericidal/fungicidal concentration of the extracts against Methicillin Resistant Staphylococcus aureus, Vancomycin Resistant enterococci, S. aureus, H. pylori, C. albicans and C. krusei ranged from 5 mg/mL to 1.25 mg/mL. The results support the use of Anthocleista djalonensis in traditional medicine. VL - 3 IS - 5 ER -
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