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Isolation, Identification and Characterization of a Lipase Producing Pseudomonas

Received: 20 October 2018     Accepted: 9 November 2018     Published: 17 December 2018
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Abstract

Lipases are hydrolytic enzymes and catalyze the hydrolysis of long-chain triacylglycerols into glycerol and fatty acid. Lipases are produced by plants, animals and microorganisms including bacteria and fungi. However microbial lipases, especially from bacteria, are more useful than their plant and animal derivatives because of several important properties. The primary goals of this research work is to isolate and identify a lipase producing Pseudomonas species from waste water samples collected from Dir lower, Peshawar and Kohat Khyber pakhtunkhwa Pakistan. The isolated bacteria were identified as Pseudomonas biochemically. The other purposes of this study are production, partial purification, characterization of lipase activity at different pH and incubation time, production and determination of molecular weight analysis. The lipase was partially purified up to 30% saturation using ammonium sulphate precipitation. Purity of lipases was cheeked by SDS-PAGE, showing the same banding pattern of all the lipases and the molecular weight were determined approximately 50kDA by comparing with protein marker bands. Spectrophotometric lipase assay was used for enzyme characterization. All the 7 isolates shows maximum activity at pH 7 after 48 hours of incubation and 37°C. Among all the isolates, isolate HSWPC shows highest activity of 110.11U /ml at pH 7 after 48 hours of incubation and 37°C. Pseudomonas lipases are widely used in food industry, detergent, paper, textile, leather and pharmaceutical industries because of their stability, selectivity and broad substrate specificity.

Published in Journal of Biomaterials (Volume 2, Issue 2)
DOI 10.11648/j.jb.20180202.15
Page(s) 51-57
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Lipases, Pseudomonas, Production, Partial Purification, Molecular Characterization, Lipase Assay

References
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  • APA Style

    Saadatullah, Ijaz Malook, Mehmood Jan, Waheedullah, Noor Muhammmad, et al. (2018). Isolation, Identification and Characterization of a Lipase Producing Pseudomonas. Journal of Biomaterials, 2(2), 51-57. https://doi.org/10.11648/j.jb.20180202.15

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    ACS Style

    Saadatullah; Ijaz Malook; Mehmood Jan; Waheedullah; Noor Muhammmad, et al. Isolation, Identification and Characterization of a Lipase Producing Pseudomonas. J. Biomater. 2018, 2(2), 51-57. doi: 10.11648/j.jb.20180202.15

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    AMA Style

    Saadatullah, Ijaz Malook, Mehmood Jan, Waheedullah, Noor Muhammmad, et al. Isolation, Identification and Characterization of a Lipase Producing Pseudomonas. J Biomater. 2018;2(2):51-57. doi: 10.11648/j.jb.20180202.15

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  • @article{10.11648/j.jb.20180202.15,
      author = {Saadatullah and Ijaz Malook and Mehmood Jan and Waheedullah and Noor Muhammmad and Zia ur Rehman},
      title = {Isolation, Identification and Characterization of a Lipase Producing Pseudomonas},
      journal = {Journal of Biomaterials},
      volume = {2},
      number = {2},
      pages = {51-57},
      doi = {10.11648/j.jb.20180202.15},
      url = {https://doi.org/10.11648/j.jb.20180202.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jb.20180202.15},
      abstract = {Lipases are hydrolytic enzymes and catalyze the hydrolysis of long-chain triacylglycerols into glycerol and fatty acid. Lipases are produced by plants, animals and microorganisms including bacteria and fungi. However microbial lipases, especially from bacteria, are more useful than their plant and animal derivatives because of several important properties. The primary goals of this research work is to isolate and identify a lipase producing Pseudomonas species from waste water samples collected from Dir lower, Peshawar and Kohat Khyber pakhtunkhwa Pakistan. The isolated bacteria were identified as Pseudomonas biochemically. The other purposes of this study are production, partial purification, characterization of lipase activity at different pH and incubation time, production and determination of molecular weight analysis. The lipase was partially purified up to 30% saturation using ammonium sulphate precipitation. Purity of lipases was cheeked by SDS-PAGE, showing the same banding pattern of all the lipases and the molecular weight were determined approximately 50kDA by comparing with protein marker bands. Spectrophotometric lipase assay was used for enzyme characterization. All the 7 isolates shows maximum activity at pH 7 after 48 hours of incubation and 37°C. Among all the isolates, isolate HSWPC shows highest activity of 110.11U /ml at pH 7 after 48 hours of incubation and 37°C. Pseudomonas lipases are widely used in food industry, detergent, paper, textile, leather and pharmaceutical industries because of their stability, selectivity and broad substrate specificity.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Isolation, Identification and Characterization of a Lipase Producing Pseudomonas
    AU  - Saadatullah
    AU  - Ijaz Malook
    AU  - Mehmood Jan
    AU  - Waheedullah
    AU  - Noor Muhammmad
    AU  - Zia ur Rehman
    Y1  - 2018/12/17
    PY  - 2018
    N1  - https://doi.org/10.11648/j.jb.20180202.15
    DO  - 10.11648/j.jb.20180202.15
    T2  - Journal of Biomaterials
    JF  - Journal of Biomaterials
    JO  - Journal of Biomaterials
    SP  - 51
    EP  - 57
    PB  - Science Publishing Group
    SN  - 2640-2629
    UR  - https://doi.org/10.11648/j.jb.20180202.15
    AB  - Lipases are hydrolytic enzymes and catalyze the hydrolysis of long-chain triacylglycerols into glycerol and fatty acid. Lipases are produced by plants, animals and microorganisms including bacteria and fungi. However microbial lipases, especially from bacteria, are more useful than their plant and animal derivatives because of several important properties. The primary goals of this research work is to isolate and identify a lipase producing Pseudomonas species from waste water samples collected from Dir lower, Peshawar and Kohat Khyber pakhtunkhwa Pakistan. The isolated bacteria were identified as Pseudomonas biochemically. The other purposes of this study are production, partial purification, characterization of lipase activity at different pH and incubation time, production and determination of molecular weight analysis. The lipase was partially purified up to 30% saturation using ammonium sulphate precipitation. Purity of lipases was cheeked by SDS-PAGE, showing the same banding pattern of all the lipases and the molecular weight were determined approximately 50kDA by comparing with protein marker bands. Spectrophotometric lipase assay was used for enzyme characterization. All the 7 isolates shows maximum activity at pH 7 after 48 hours of incubation and 37°C. Among all the isolates, isolate HSWPC shows highest activity of 110.11U /ml at pH 7 after 48 hours of incubation and 37°C. Pseudomonas lipases are widely used in food industry, detergent, paper, textile, leather and pharmaceutical industries because of their stability, selectivity and broad substrate specificity.
    VL  - 2
    IS  - 2
    ER  - 

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Author Information
  • Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan

  • Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan

  • Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan

  • Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan

  • Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan

  • Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan

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