Chemical Composition and Antimicrobial Activity of Essential Oil of <i>Belpharis linariifolia</i>

Noha Ali Ibrahim; Safa Khalid Musa; Shima Mohammed Yassin; Nosiba Hashim Abuniama; Sufyan Awadalkareem; Alsiddig Osama; Mohamed N. Abdalaziz

doi:doi:10.11648/j.ijsts.20170504.12

Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia

Noha Ali Ibrahim, Safa Khalid Musa, Shima Mohammed Yassin, Nosiba Hashim Abuniama, Sufyan Awadalkareem, Alsiddig Osama, Mohamed N. Abdalaziz

Published in International Journal of Science, Technology and Society (Volume 5, Issue 4)

Received: 28 February 2017 Accepted: 19 April 2017 Published: 3 June 2017

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Abstract

The study was aimed to investigate essential oil chemical composition and antimicrobial activities of essential oils extracted from seeds of Belpharis linariifolia. The oil was extracted according to the method described by Harborne (1984). and analyzed by Fourier Transform Infrared Spectrophotometer (FTIR) and Gas Chromatography Mass Spectrometry (GC/MS) techniques to determine the chemical composition of the volatile fraction and identify their chemo-types. The essential oil of Belpharis linariifolia seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the paper disc diffusion method. Twenty two components were identified in the essential oil of Belpharis linariifolia representing 82.87% of the total components, the major compounds were Acetic acid (11.60%), 4-acetyl-2-isopropyl-5,5-dimethyltetrahydrofuran-2-yl (11.60%), 3-Cyano-2-Oxa-1-Ethoxyadamanane (13.09%), Ethyl 3-methyl-2-oxobutyrate (15.49%), Hexatriacontane (8.18%) and Dotriacontane (16.03%). Antimicrobial activity of essential oil of Belpharis linariifolia dissolved in methanol (1:10), showed low activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (14 & 11 mm). It also showed against Gram positive bacteria (S. aureus & B. subtilis) (11.5 & 14 mm) and against (C. albicans) (zero mm). This study conducted for essential oil of Belpharis linariifolia seeds presence of variable compounds with diverse structures and low antimicrobial activity.

Published in International Journal of Science, Technology and Society (Volume 5, Issue 4)

DOI 10.11648/j.ijsts.20170504.12

Page(s) 62-66

Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

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Copyright © The Author(s), 2017. Published by Science Publishing Group

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Keywords

in-vitro, FT-IR, Gas Chromatography–Mass Spectrometry (GC-MS), Antimicrobial Activity, Essential Oils, Belpharis linariifolia (Seeds)

References

[1] Srivastava J, Lambert J and Vietmeyer N. Medicinal plants:An expanding role in development. World Bank Technical, 1996, 320.

[2] Parekh J and Chanda S. In vitro antimicrobial activity of Trapa natans L. fruit rind extracted in different solvents. African Journal of Biotechnology, 6 (6), 2007, 760-770.

[3] Rinoldi MG. Problems in the diagnosis of invasive fungaldiseases. Review of infectious diseases, 13, 1991, 493-495.

[4] Meng JH, Zhao SH, Doyle MP, Joseph SW. Antibioticresistance of Escherichia coli O157: H7 and O157: NM isolatedfrom animals, food and humans. Journal of Food Protection, 61, 1998, 1511-1514.

[5] Perreten V, Giampa N, Schuler-Schmid U, Teuber M. Antibiotic resistance genes in coagulase-negativestaphylococci isolated from food. Systematic and AppliedMicrobiology, 21, 1998, 113-120.

[6] Kallio, H.; Kerrola, K.; Alhonmaki, P. Carvone and limonene in caraway fruits (Carum carvi L.) analyzed by supercritical carbon dioxide extraction-gas chromatography. J. Agric. Food Chem.1994, 42, 2478-2485.

[7] Burkil. H. M. The Useful Plants of West Tropical Africa. Blepharis linariifolia. Royal Botanic Garden; Kew. (1985-2004). [updated 2016 March 02; cited 2016 February 12] Available from: http://tropical.theferns.info/viewtropical.php?id=Blepharis+linariifolia.

[8] Wamtinga Richard Sawadogo, Aline Meda, Charles Euloge Lamien and Martin Kiendrebeogo. Phenolic Content and Antioxidant Activity of Six Acanthaceae from Burkina Faso. Journal of Biological Sciences. 2006; 6(2): 249-252.

[9] National Committee for Clinical Laboratory Standards (NCCLS) (1999). Performance standards for antimicrobial susceptibility testing; ninth informational supplement. Wayne, Pensilvania document M100-S9, Vol. 19. No. 1.

[10] Silva Junior AA, Vizotto VJ, Giongi E, Macedo SG, Marques LF (1994). Plantas medicinais, caracterização e cultivo. EPAGRI. Bol. Técnico Florianópolis, pp. 68: 1-71.

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Noha Ali Ibrahim, Safa Khalid Musa, Shima Mohammed Yassin, Nosiba Hashim Abuniama, Sufyan Awadalkareem, et al. (2017). Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia. International Journal of Science, Technology and Society, 5(4), 62-66. https://doi.org/10.11648/j.ijsts.20170504.12

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Noha Ali Ibrahim; Safa Khalid Musa; Shima Mohammed Yassin; Nosiba Hashim Abuniama; Sufyan Awadalkareem, et al. Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia. Int. J. Sci. Technol. Soc. 2017, 5(4), 62-66. doi: 10.11648/j.ijsts.20170504.12

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Noha Ali Ibrahim, Safa Khalid Musa, Shima Mohammed Yassin, Nosiba Hashim Abuniama, Sufyan Awadalkareem, et al. Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia. Int J Sci Technol Soc. 2017;5(4):62-66. doi: 10.11648/j.ijsts.20170504.12

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@article{10.11648/j.ijsts.20170504.12, author = {Noha Ali Ibrahim and Safa Khalid Musa and Shima Mohammed Yassin and Nosiba Hashim Abuniama and Sufyan Awadalkareem and Alsiddig Osama and Mohamed N. Abdalaziz}, title = {Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia}, journal = {International Journal of Science, Technology and Society}, volume = {5}, number = {4}, pages = {62-66}, doi = {10.11648/j.ijsts.20170504.12}, url = {https://doi.org/10.11648/j.ijsts.20170504.12}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijsts.20170504.12}, abstract = {The study was aimed to investigate essential oil chemical composition and antimicrobial activities of essential oils extracted from seeds of Belpharis linariifolia. The oil was extracted according to the method described by Harborne (1984). and analyzed by Fourier Transform Infrared Spectrophotometer (FTIR) and Gas Chromatography Mass Spectrometry (GC/MS) techniques to determine the chemical composition of the volatile fraction and identify their chemo-types. The essential oil of Belpharis linariifolia seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the paper disc diffusion method. Twenty two components were identified in the essential oil of Belpharis linariifolia representing 82.87% of the total components, the major compounds were Acetic acid (11.60%), 4-acetyl-2-isopropyl-5,5-dimethyltetrahydrofuran-2-yl (11.60%), 3-Cyano-2-Oxa-1-Ethoxyadamanane (13.09%), Ethyl 3-methyl-2-oxobutyrate (15.49%), Hexatriacontane (8.18%) and Dotriacontane (16.03%). Antimicrobial activity of essential oil of Belpharis linariifolia dissolved in methanol (1:10), showed low activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (14 & 11 mm). It also showed against Gram positive bacteria (S. aureus & B. subtilis) (11.5 & 14 mm) and against (C. albicans) (zero mm). This study conducted for essential oil of Belpharis linariifolia seeds presence of variable compounds with diverse structures and low antimicrobial activity.}, year = {2017} }

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TY - JOUR T1 - Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia AU - Noha Ali Ibrahim AU - Safa Khalid Musa AU - Shima Mohammed Yassin AU - Nosiba Hashim Abuniama AU - Sufyan Awadalkareem AU - Alsiddig Osama AU - Mohamed N. Abdalaziz Y1 - 2017/06/03 PY - 2017 N1 - https://doi.org/10.11648/j.ijsts.20170504.12 DO - 10.11648/j.ijsts.20170504.12 T2 - International Journal of Science, Technology and Society JF - International Journal of Science, Technology and Society JO - International Journal of Science, Technology and Society SP - 62 EP - 66 PB - Science Publishing Group SN - 2330-7420 UR - https://doi.org/10.11648/j.ijsts.20170504.12 AB - The study was aimed to investigate essential oil chemical composition and antimicrobial activities of essential oils extracted from seeds of Belpharis linariifolia. The oil was extracted according to the method described by Harborne (1984). and analyzed by Fourier Transform Infrared Spectrophotometer (FTIR) and Gas Chromatography Mass Spectrometry (GC/MS) techniques to determine the chemical composition of the volatile fraction and identify their chemo-types. The essential oil of Belpharis linariifolia seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the paper disc diffusion method. Twenty two components were identified in the essential oil of Belpharis linariifolia representing 82.87% of the total components, the major compounds were Acetic acid (11.60%), 4-acetyl-2-isopropyl-5,5-dimethyltetrahydrofuran-2-yl (11.60%), 3-Cyano-2-Oxa-1-Ethoxyadamanane (13.09%), Ethyl 3-methyl-2-oxobutyrate (15.49%), Hexatriacontane (8.18%) and Dotriacontane (16.03%). Antimicrobial activity of essential oil of Belpharis linariifolia dissolved in methanol (1:10), showed low activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (14 & 11 mm). It also showed against Gram positive bacteria (S. aureus & B. subtilis) (11.5 & 14 mm) and against (C. albicans) (zero mm). This study conducted for essential oil of Belpharis linariifolia seeds presence of variable compounds with diverse structures and low antimicrobial activity. VL - 5 IS - 4 ER -

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Author Information

Noha Ali Ibrahim

Chemistry Department, Omdurman Islamic University, Khartoum, Sudan

Safa Khalid Musa

Chemistry Department, Omdurman Islamic University, Khartoum, Sudan

Shima Mohammed Yassin

Chemistry Department, Omdurman Islamic University, Khartoum, Sudan

Nosiba Hashim Abuniama

Chemistry Department, Omdurman Islamic University, Khartoum, Sudan

Sufyan Awadalkareem

Chemistry Department, Omdurman Islamic University, Khartoum, Sudan

Alsiddig Osama

Chemistry Department, Omdurman Islamic University, Khartoum, Sudan

Mohamed N. Abdalaziz

Medical Biochemistry Research Department, Medicinal and Aromatic Plants Research Institute, National Center for Research, Khartoum, Sudan

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Noha Ali Ibrahim, Safa Khalid Musa, Shima Mohammed Yassin, Nosiba Hashim Abuniama, Sufyan Awadalkareem, et al. (2017). Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia. International Journal of Science, Technology and Society, 5(4), 62-66. https://doi.org/10.11648/j.ijsts.20170504.12

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Noha Ali Ibrahim; Safa Khalid Musa; Shima Mohammed Yassin; Nosiba Hashim Abuniama; Sufyan Awadalkareem, et al. Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia. Int. J. Sci. Technol. Soc. 2017, 5(4), 62-66. doi: 10.11648/j.ijsts.20170504.12

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AMA Style

Noha Ali Ibrahim, Safa Khalid Musa, Shima Mohammed Yassin, Nosiba Hashim Abuniama, Sufyan Awadalkareem, et al. Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia. Int J Sci Technol Soc. 2017;5(4):62-66. doi: 10.11648/j.ijsts.20170504.12

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@article{10.11648/j.ijsts.20170504.12, author = {Noha Ali Ibrahim and Safa Khalid Musa and Shima Mohammed Yassin and Nosiba Hashim Abuniama and Sufyan Awadalkareem and Alsiddig Osama and Mohamed N. Abdalaziz}, title = {Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia}, journal = {International Journal of Science, Technology and Society}, volume = {5}, number = {4}, pages = {62-66}, doi = {10.11648/j.ijsts.20170504.12}, url = {https://doi.org/10.11648/j.ijsts.20170504.12}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijsts.20170504.12}, abstract = {The study was aimed to investigate essential oil chemical composition and antimicrobial activities of essential oils extracted from seeds of Belpharis linariifolia. The oil was extracted according to the method described by Harborne (1984). and analyzed by Fourier Transform Infrared Spectrophotometer (FTIR) and Gas Chromatography Mass Spectrometry (GC/MS) techniques to determine the chemical composition of the volatile fraction and identify their chemo-types. The essential oil of Belpharis linariifolia seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the paper disc diffusion method. Twenty two components were identified in the essential oil of Belpharis linariifolia representing 82.87% of the total components, the major compounds were Acetic acid (11.60%), 4-acetyl-2-isopropyl-5,5-dimethyltetrahydrofuran-2-yl (11.60%), 3-Cyano-2-Oxa-1-Ethoxyadamanane (13.09%), Ethyl 3-methyl-2-oxobutyrate (15.49%), Hexatriacontane (8.18%) and Dotriacontane (16.03%). Antimicrobial activity of essential oil of Belpharis linariifolia dissolved in methanol (1:10), showed low activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (14 & 11 mm). It also showed against Gram positive bacteria (S. aureus & B. subtilis) (11.5 & 14 mm) and against (C. albicans) (zero mm). This study conducted for essential oil of Belpharis linariifolia seeds presence of variable compounds with diverse structures and low antimicrobial activity.}, year = {2017} }

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TY - JOUR T1 - Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia AU - Noha Ali Ibrahim AU - Safa Khalid Musa AU - Shima Mohammed Yassin AU - Nosiba Hashim Abuniama AU - Sufyan Awadalkareem AU - Alsiddig Osama AU - Mohamed N. Abdalaziz Y1 - 2017/06/03 PY - 2017 N1 - https://doi.org/10.11648/j.ijsts.20170504.12 DO - 10.11648/j.ijsts.20170504.12 T2 - International Journal of Science, Technology and Society JF - International Journal of Science, Technology and Society JO - International Journal of Science, Technology and Society SP - 62 EP - 66 PB - Science Publishing Group SN - 2330-7420 UR - https://doi.org/10.11648/j.ijsts.20170504.12 AB - The study was aimed to investigate essential oil chemical composition and antimicrobial activities of essential oils extracted from seeds of Belpharis linariifolia. The oil was extracted according to the method described by Harborne (1984). and analyzed by Fourier Transform Infrared Spectrophotometer (FTIR) and Gas Chromatography Mass Spectrometry (GC/MS) techniques to determine the chemical composition of the volatile fraction and identify their chemo-types. The essential oil of Belpharis linariifolia seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the paper disc diffusion method. Twenty two components were identified in the essential oil of Belpharis linariifolia representing 82.87% of the total components, the major compounds were Acetic acid (11.60%), 4-acetyl-2-isopropyl-5,5-dimethyltetrahydrofuran-2-yl (11.60%), 3-Cyano-2-Oxa-1-Ethoxyadamanane (13.09%), Ethyl 3-methyl-2-oxobutyrate (15.49%), Hexatriacontane (8.18%) and Dotriacontane (16.03%). Antimicrobial activity of essential oil of Belpharis linariifolia dissolved in methanol (1:10), showed low activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (14 & 11 mm). It also showed against Gram positive bacteria (S. aureus & B. subtilis) (11.5 & 14 mm) and against (C. albicans) (zero mm). This study conducted for essential oil of Belpharis linariifolia seeds presence of variable compounds with diverse structures and low antimicrobial activity. VL - 5 IS - 4 ER -

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Published in	International Journal of Science, Technology and Society (Volume 5, Issue 4)
DOI	10.11648/j.ijsts.20170504.12
Page(s)	62-66
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

[1]	Srivastava J, Lambert J and Vietmeyer N. Medicinal plants:An expanding role in development. World Bank Technical, 1996, 320.
[2]	Parekh J and Chanda S. In vitro antimicrobial activity of Trapa natans L. fruit rind extracted in different solvents. African Journal of Biotechnology, 6 (6), 2007, 760-770.
[3]	Rinoldi MG. Problems in the diagnosis of invasive fungaldiseases. Review of infectious diseases, 13, 1991, 493-495.
[4]	Meng JH, Zhao SH, Doyle MP, Joseph SW. Antibioticresistance of Escherichia coli O157: H7 and O157: NM isolatedfrom animals, food and humans. Journal of Food Protection, 61, 1998, 1511-1514.
[5]	Perreten V, Giampa N, Schuler-Schmid U, Teuber M. Antibiotic resistance genes in coagulase-negativestaphylococci isolated from food. Systematic and AppliedMicrobiology, 21, 1998, 113-120.
[6]	Kallio, H.; Kerrola, K.; Alhonmaki, P. Carvone and limonene in caraway fruits (Carum carvi L.) analyzed by supercritical carbon dioxide extraction-gas chromatography. J. Agric. Food Chem.1994, 42, 2478-2485.
[7]	Burkil. H. M. The Useful Plants of West Tropical Africa. Blepharis linariifolia. Royal Botanic Garden; Kew. (1985-2004). [updated 2016 March 02; cited 2016 February 12] Available from: http://tropical.theferns.info/viewtropical.php?id=Blepharis+linariifolia.
[8]	Wamtinga Richard Sawadogo, Aline Meda, Charles Euloge Lamien and Martin Kiendrebeogo. Phenolic Content and Antioxidant Activity of Six Acanthaceae from Burkina Faso. Journal of Biological Sciences. 2006; 6(2): 249-252.
[9]	National Committee for Clinical Laboratory Standards (NCCLS) (1999). Performance standards for antimicrobial susceptibility testing; ninth informational supplement. Wayne, Pensilvania document M100-S9, Vol. 19. No. 1.
[10]	Silva Junior AA, Vizotto VJ, Giongi E, Macedo SG, Marques LF (1994). Plantas medicinais, caracterização e cultivo. EPAGRI. Bol. Técnico Florianópolis, pp. 68: 1-71.