Effect of Black Walnut (<i>Tetracarpidium conophorum</i>) Leaf Extract on the Reproductive Organ of Male Albino Rats

Ojobor Charles Chijioke; Anosike Chioma Assumpta; Ezeanyika Lawrence Uchenna Sunday

doi:doi:10.11648/j.ijhnm.20170301.12

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Effect of Black Walnut (Tetracarpidium conophorum) Leaf Extract on the Reproductive Organ of Male Albino Rats

Ojobor Charles Chijioke, Anosike Chioma Assumpta, Ezeanyika Lawrence Uchenna Sunday

Published in International Journal of Homeopathy & Natural Medicines (Volume 3, Issue 1)

Received: 19 May 2017 Accepted: 23 June 2017 Published: 18 October 2017

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Abstract

This study determined the effect of Tetracarpidium conophorum (black walnut) leaf extract on the male reproductive organs of albino rats. The effects of the leaf extracts were determined on the Epididymal sperm concentration, Testicular histology, and on testosterone concentration in the rat serum by a micro plate enzyme immunoassay (Testosterone assay). A total of sixteen (16) male albino wistar rats were divided into four (1, 2, 3 and 4) groups of four rats each. Group 1 served as the control and was fed with normal diet only, while groups 2, 3 and 4 were fed with 200, 400 and 600 mg/kg body weight (BW) of the extract for a period of two weeks. The Epididymal sperm concentration were not significantly affected (p>0.05) across the groups. The level of testosterone for the treatment groups 2 and 4 showed no significant difference (p>0.05) compared to the control while group 4 showed significant increase compared to that of the control (p<0.05). Pathologic changes were observed in testicular histology across the treatment groups. Robust seminiferous tubular lumen containing sperm cells and increased production of Leydig cells and Sertoli cells were observed across different treatment groups compared to that of the control.

Published in	International Journal of Homeopathy & Natural Medicines (Volume 3, Issue 1)
DOI	10.11648/j.ijhnm.20170301.12
Page(s)	9-14
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Tetracarpidium conophorum, Sperm, Male Reproductive Organ, Testicular Histology, Rats

References

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APA Style

Ojobor Charles Chijioke, Anosike Chioma Assumpta, Ezeanyika Lawrence Uchenna Sunday. (2017). Effect of Black Walnut (Tetracarpidium conophorum) Leaf Extract on the Reproductive Organ of Male Albino Rats. International Journal of Homeopathy & Natural Medicines, 3(1), 9-14. https://doi.org/10.11648/j.ijhnm.20170301.12

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ACS Style

Ojobor Charles Chijioke; Anosike Chioma Assumpta; Ezeanyika Lawrence Uchenna Sunday. Effect of Black Walnut (Tetracarpidium conophorum) Leaf Extract on the Reproductive Organ of Male Albino Rats. Int. J. Homeopathy Nat. Med. 2017, 3(1), 9-14. doi: 10.11648/j.ijhnm.20170301.12

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AMA Style

Ojobor Charles Chijioke, Anosike Chioma Assumpta, Ezeanyika Lawrence Uchenna Sunday. Effect of Black Walnut (Tetracarpidium conophorum) Leaf Extract on the Reproductive Organ of Male Albino Rats. Int J Homeopathy Nat Med. 2017;3(1):9-14. doi: 10.11648/j.ijhnm.20170301.12

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@article{10.11648/j.ijhnm.20170301.12,
  author = {Ojobor Charles Chijioke and Anosike Chioma Assumpta and Ezeanyika Lawrence Uchenna Sunday},
  title = {Effect of Black Walnut (Tetracarpidium conophorum) Leaf Extract on the Reproductive Organ of Male Albino Rats},
  journal = {International Journal of Homeopathy & Natural Medicines},
  volume = {3},
  number = {1},
  pages = {9-14},
  doi = {10.11648/j.ijhnm.20170301.12},
  url = {https://doi.org/10.11648/j.ijhnm.20170301.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijhnm.20170301.12},
  abstract = {This study determined the effect of Tetracarpidium conophorum (black walnut) leaf extract on the male reproductive organs of albino rats. The effects of the leaf extracts were determined on the Epididymal sperm concentration, Testicular histology, and on testosterone concentration in the rat serum by a micro plate enzyme immunoassay (Testosterone assay). A total of sixteen (16) male albino wistar rats were divided into four (1, 2, 3 and 4) groups of four rats each. Group 1 served as the control and was fed with normal diet only, while groups 2, 3 and 4 were fed with 200, 400 and 600 mg/kg body weight (BW) of the extract for a period of two weeks. The Epididymal sperm concentration were not significantly affected (p>0.05) across the groups. The level of testosterone for the treatment groups 2 and 4 showed no significant difference (p>0.05) compared to the control while group 4 showed significant increase compared to that of the control (p<0.05). Pathologic changes were observed in testicular histology across the treatment groups. Robust seminiferous tubular lumen containing sperm cells and increased production of Leydig cells and Sertoli cells were observed across different treatment groups compared to that of the control.},
 year = {2017}
}

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TY  - JOUR
T1  - Effect of Black Walnut (Tetracarpidium conophorum) Leaf Extract on the Reproductive Organ of Male Albino Rats
AU  - Ojobor Charles Chijioke
AU  - Anosike Chioma Assumpta
AU  - Ezeanyika Lawrence Uchenna Sunday
Y1  - 2017/10/18
PY  - 2017
N1  - https://doi.org/10.11648/j.ijhnm.20170301.12
DO  - 10.11648/j.ijhnm.20170301.12
T2  - International Journal of Homeopathy & Natural Medicines
JF  - International Journal of Homeopathy & Natural Medicines
JO  - International Journal of Homeopathy & Natural Medicines
SP  - 9
EP  - 14
PB  - Science Publishing Group
SN  - 2472-2316
UR  - https://doi.org/10.11648/j.ijhnm.20170301.12
AB  - This study determined the effect of Tetracarpidium conophorum (black walnut) leaf extract on the male reproductive organs of albino rats. The effects of the leaf extracts were determined on the Epididymal sperm concentration, Testicular histology, and on testosterone concentration in the rat serum by a micro plate enzyme immunoassay (Testosterone assay). A total of sixteen (16) male albino wistar rats were divided into four (1, 2, 3 and 4) groups of four rats each. Group 1 served as the control and was fed with normal diet only, while groups 2, 3 and 4 were fed with 200, 400 and 600 mg/kg body weight (BW) of the extract for a period of two weeks. The Epididymal sperm concentration were not significantly affected (p>0.05) across the groups. The level of testosterone for the treatment groups 2 and 4 showed no significant difference (p>0.05) compared to the control while group 4 showed significant increase compared to that of the control (p<0.05). Pathologic changes were observed in testicular histology across the treatment groups. Robust seminiferous tubular lumen containing sperm cells and increased production of Leydig cells and Sertoli cells were observed across different treatment groups compared to that of the control.
VL  - 3
IS  - 1
ER  -

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Author Information

Ojobor Charles Chijioke

Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
Anosike Chioma Assumpta

Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
Ezeanyika Lawrence Uchenna Sunday

Department of Biochemistry, University of Nigeria, Nsukka, Nigeria

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APA Style

Ojobor Charles Chijioke, Anosike Chioma Assumpta, Ezeanyika Lawrence Uchenna Sunday. (2017). Effect of Black Walnut (Tetracarpidium conophorum) Leaf Extract on the Reproductive Organ of Male Albino Rats. International Journal of Homeopathy & Natural Medicines, 3(1), 9-14. https://doi.org/10.11648/j.ijhnm.20170301.12

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ACS Style

Ojobor Charles Chijioke; Anosike Chioma Assumpta; Ezeanyika Lawrence Uchenna Sunday. Effect of Black Walnut (Tetracarpidium conophorum) Leaf Extract on the Reproductive Organ of Male Albino Rats. Int. J. Homeopathy Nat. Med. 2017, 3(1), 9-14. doi: 10.11648/j.ijhnm.20170301.12

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AMA Style

Ojobor Charles Chijioke, Anosike Chioma Assumpta, Ezeanyika Lawrence Uchenna Sunday. Effect of Black Walnut (Tetracarpidium conophorum) Leaf Extract on the Reproductive Organ of Male Albino Rats. Int J Homeopathy Nat Med. 2017;3(1):9-14. doi: 10.11648/j.ijhnm.20170301.12

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@article{10.11648/j.ijhnm.20170301.12,
  author = {Ojobor Charles Chijioke and Anosike Chioma Assumpta and Ezeanyika Lawrence Uchenna Sunday},
  title = {Effect of Black Walnut (Tetracarpidium conophorum) Leaf Extract on the Reproductive Organ of Male Albino Rats},
  journal = {International Journal of Homeopathy & Natural Medicines},
  volume = {3},
  number = {1},
  pages = {9-14},
  doi = {10.11648/j.ijhnm.20170301.12},
  url = {https://doi.org/10.11648/j.ijhnm.20170301.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijhnm.20170301.12},
  abstract = {This study determined the effect of Tetracarpidium conophorum (black walnut) leaf extract on the male reproductive organs of albino rats. The effects of the leaf extracts were determined on the Epididymal sperm concentration, Testicular histology, and on testosterone concentration in the rat serum by a micro plate enzyme immunoassay (Testosterone assay). A total of sixteen (16) male albino wistar rats were divided into four (1, 2, 3 and 4) groups of four rats each. Group 1 served as the control and was fed with normal diet only, while groups 2, 3 and 4 were fed with 200, 400 and 600 mg/kg body weight (BW) of the extract for a period of two weeks. The Epididymal sperm concentration were not significantly affected (p>0.05) across the groups. The level of testosterone for the treatment groups 2 and 4 showed no significant difference (p>0.05) compared to the control while group 4 showed significant increase compared to that of the control (p<0.05). Pathologic changes were observed in testicular histology across the treatment groups. Robust seminiferous tubular lumen containing sperm cells and increased production of Leydig cells and Sertoli cells were observed across different treatment groups compared to that of the control.},
 year = {2017}
}

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TY  - JOUR
T1  - Effect of Black Walnut (Tetracarpidium conophorum) Leaf Extract on the Reproductive Organ of Male Albino Rats
AU  - Ojobor Charles Chijioke
AU  - Anosike Chioma Assumpta
AU  - Ezeanyika Lawrence Uchenna Sunday
Y1  - 2017/10/18
PY  - 2017
N1  - https://doi.org/10.11648/j.ijhnm.20170301.12
DO  - 10.11648/j.ijhnm.20170301.12
T2  - International Journal of Homeopathy & Natural Medicines
JF  - International Journal of Homeopathy & Natural Medicines
JO  - International Journal of Homeopathy & Natural Medicines
SP  - 9
EP  - 14
PB  - Science Publishing Group
SN  - 2472-2316
UR  - https://doi.org/10.11648/j.ijhnm.20170301.12
AB  - This study determined the effect of Tetracarpidium conophorum (black walnut) leaf extract on the male reproductive organs of albino rats. The effects of the leaf extracts were determined on the Epididymal sperm concentration, Testicular histology, and on testosterone concentration in the rat serum by a micro plate enzyme immunoassay (Testosterone assay). A total of sixteen (16) male albino wistar rats were divided into four (1, 2, 3 and 4) groups of four rats each. Group 1 served as the control and was fed with normal diet only, while groups 2, 3 and 4 were fed with 200, 400 and 600 mg/kg body weight (BW) of the extract for a period of two weeks. The Epididymal sperm concentration were not significantly affected (p>0.05) across the groups. The level of testosterone for the treatment groups 2 and 4 showed no significant difference (p>0.05) compared to the control while group 4 showed significant increase compared to that of the control (p<0.05). Pathologic changes were observed in testicular histology across the treatment groups. Robust seminiferous tubular lumen containing sperm cells and increased production of Leydig cells and Sertoli cells were observed across different treatment groups compared to that of the control.
VL  - 3
IS  - 1
ER  -

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