Application of SSR Molecular Marker Technology in Rice Variety Identification

Shasha Zhang

doi:doi:10.11648/j.ijaas.20241006.16

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Application of SSR Molecular Marker Technology in Rice Variety Identification

Shasha Zhang^*

Published in International Journal of Applied Agricultural Sciences (Volume 10, Issue 6)

Received: 12 November 2024 Accepted: 25 November 2024 Published: 10 December 2024

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Abstract

This study mainlydescribes the principles and advantages of SSR molecular marker technology, focusing on the analysis and discussion of the application of SSR molecular marker technology in rice variety identification, as well as the technical points of using SSR molecular marker technology to identify rice varieties, including the study of rapid DNA extraction methods, optimization of PCR amplification procedures and reaction systems, improvement of PCR amplification product detection methods, primer screening and construction of DNA fingerprint maps.

Published in	International Journal of Applied Agricultural Sciences (Volume 10, Issue 6)
DOI	10.11648/j.ijaas.20241006.16
Page(s)	328-332
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Rice, Paddy, Variety Identification, SSR Molecular Marker

References

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[2]	Zheng Xianghua, Ye Junhua, Cheng Chaoping, et al. Identification of indica and japonica rice varieties using SNP markers. Acta Agronomica Sinica, 2022, 48(2): 342-352.
[3]	Fang Xuanjun, Liu Siheng, Jiang Shuye. DNA molecular markers for variety purity and authenticity and their applications. Journal of Agricultural Biotechnology, 2000(2): 106-110.
[4]	Liang Mingshan, Zeng Yu, Zhou Xiang, et al. Genetic markers and their applications in crop variety identification. Botanical Bulletin, 2001, 18(3): 257-265.
[5]	Yu Yonghong, Li Yunhai, Ma Rongrong, et al. Establishment of fingerprint of Ning 2A using microsatellite DNA markers. Chinese Journal of Rice Science, 2001, (03): 56-58. https://doi.org/10.16819/j.1001-7216.2001.03.012
[6]	Li Ran, Qian Qian, Gao Zhenyu. Research progress on genetics and breeding improvement of rice quality. Biotechnology Bulletin, 2022, 38(4): 4-19. https://doi.org/10.13560/j.cnki.biotech.bull.1985.2021-1598
[7]	Xin Yeyun, Zhang Zhan, Xiong Yiping, et al. Application of SSR molecular markers to identify super hybrid rice combinations and their purity. Chinese Journal of Rice Science, 2005, 19(2): 95-100. https://doi.org/10.16819/j.1001-7216.2005.02.001
[8]	Tan Yujun, Xiao Cenglin. Application of molecular markers in hybrid rice seed purity identification. Crop Research, 2006(5): 409-412. https://doi.org/10.16848/j.cnki.issn.1001-5280.2006.05.010
[9]	Xiao Xiaoyu, Wang Yuping, Zhang Jianyong, et al. SSR polymorphism analysis and fingerprint map construction and application of main hybrid rice parents in Sichuan Province. Chinese Rice Science, 2006, 20(1): 1-7. https://doi.org/10.16819/j.1001-7216.2006.01.001
[10]	Cheng Benyi, Wu Wei, Xia Junhui, et al. Preliminary construction and application of DNA fingerprint database of rice varieties in Zhejiang Province. Journal of Zhejiang Agricultural Sciences, 2009, 21(6): 555-560.
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[12]	Xiao Zifa, Zhu Keyong, Liu Zhixi, et al. Construction of DNA fingerprint of hybrid rice varieties approved by Hunan Province using SSR markers. Hybrid Rice, 2011, 26(3): 51-56. https://doi.org/10.16267/j.cnki.1005-3956.2011.03.021
[13]	Ren Hui. Application of SSR molecular markers in hybrid rice variety purity identification. Hangzhou: Zhejiang A&F University, 2013.
[14]	Zeng Xiaoshan, Peng Dan, Shi Yuanyuan, et al. Construction of rice core parent fingerprint using SSR markers. Crop Research, 2016, 30(5): 481-486+511. https://doi.org/10.16848/j.cnki.issn.1001-5280.2016.05.01
[15]	Fan Jiameng, Wang Zhiyan, Lin Huihui, et al. Study on the identification of rice seed purity by capillary multiplex electrophoresis technology. Anhui Agricultural Sciences, 2022, 50(15): 90-94.
[16]	Wang Yankun, Wei Xinyu, Hu Jie, et al. Genetic diversity analysis and fingerprint construction of 12 rice restorer lines. Subtropical Agricultural Research, 2022, 18(2): 73-78. https://doi.org/10.13321/j.cnki.subtrop.agric.res.2022.02.001
[17]	Ma Hongbo, Xu Xuming, Wei Xinyu, et al. Construction of DNA fingerprints and genetic diversity analysis of several rice varieties in Fujian Province based on SSR markers. Journal of Fujian Agricultural Sciences, 2010, 25(1): 33-38. https://doi.org/10.19303/j.issn.1008-0384.2010.01.007
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APA Style

Zhang, S. (2024). Application of SSR Molecular Marker Technology in Rice Variety Identification. International Journal of Applied Agricultural Sciences, 10(6), 328-332. https://doi.org/10.11648/j.ijaas.20241006.16

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ACS Style

Zhang, S. Application of SSR Molecular Marker Technology in Rice Variety Identification. Int. J. Appl. Agric. Sci. 2024, 10(6), 328-332. doi: 10.11648/j.ijaas.20241006.16

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AMA Style

Zhang S. Application of SSR Molecular Marker Technology in Rice Variety Identification. Int J Appl Agric Sci. 2024;10(6):328-332. doi: 10.11648/j.ijaas.20241006.16

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@article{10.11648/j.ijaas.20241006.16,
  author = {Shasha Zhang},
  title = {Application of SSR Molecular Marker Technology in Rice Variety Identification
},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {10},
  number = {6},
  pages = {328-332},
  doi = {10.11648/j.ijaas.20241006.16},
  url = {https://doi.org/10.11648/j.ijaas.20241006.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20241006.16},
  abstract = {This study mainlydescribes the principles and advantages of SSR molecular marker technology, focusing on the analysis and discussion of the application of SSR molecular marker technology in rice variety identification, as well as the technical points of using SSR molecular marker technology to identify rice varieties, including the study of rapid DNA extraction methods, optimization of PCR amplification procedures and reaction systems, improvement of PCR amplification product detection methods, primer screening and construction of DNA fingerprint maps.
},
 year = {2024}
}

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TY  - JOUR
T1  - Application of SSR Molecular Marker Technology in Rice Variety Identification

AU  - Shasha Zhang
Y1  - 2024/12/10
PY  - 2024
N1  - https://doi.org/10.11648/j.ijaas.20241006.16
DO  - 10.11648/j.ijaas.20241006.16
T2  - International Journal of Applied Agricultural Sciences
JF  - International Journal of Applied Agricultural Sciences
JO  - International Journal of Applied Agricultural Sciences
SP  - 328
EP  - 332
PB  - Science Publishing Group
SN  - 2469-7885
UR  - https://doi.org/10.11648/j.ijaas.20241006.16
AB  - This study mainlydescribes the principles and advantages of SSR molecular marker technology, focusing on the analysis and discussion of the application of SSR molecular marker technology in rice variety identification, as well as the technical points of using SSR molecular marker technology to identify rice varieties, including the study of rapid DNA extraction methods, optimization of PCR amplification procedures and reaction systems, improvement of PCR amplification product detection methods, primer screening and construction of DNA fingerprint maps.

VL  - 10
IS  - 6
ER  -

Copy | Download

Author Information

Shasha Zhang

Panjin Center for Inspection and Testing, Panjin, China

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Plain Text BibTeX RIS

APA Style

Zhang, S. (2024). Application of SSR Molecular Marker Technology in Rice Variety Identification. International Journal of Applied Agricultural Sciences, 10(6), 328-332. https://doi.org/10.11648/j.ijaas.20241006.16

Copy | Download

ACS Style

Zhang, S. Application of SSR Molecular Marker Technology in Rice Variety Identification. Int. J. Appl. Agric. Sci. 2024, 10(6), 328-332. doi: 10.11648/j.ijaas.20241006.16

Copy | Download

AMA Style

Zhang S. Application of SSR Molecular Marker Technology in Rice Variety Identification. Int J Appl Agric Sci. 2024;10(6):328-332. doi: 10.11648/j.ijaas.20241006.16

Copy | Download

@article{10.11648/j.ijaas.20241006.16,
  author = {Shasha Zhang},
  title = {Application of SSR Molecular Marker Technology in Rice Variety Identification
},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {10},
  number = {6},
  pages = {328-332},
  doi = {10.11648/j.ijaas.20241006.16},
  url = {https://doi.org/10.11648/j.ijaas.20241006.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20241006.16},
  abstract = {This study mainlydescribes the principles and advantages of SSR molecular marker technology, focusing on the analysis and discussion of the application of SSR molecular marker technology in rice variety identification, as well as the technical points of using SSR molecular marker technology to identify rice varieties, including the study of rapid DNA extraction methods, optimization of PCR amplification procedures and reaction systems, improvement of PCR amplification product detection methods, primer screening and construction of DNA fingerprint maps.
},
 year = {2024}
}

Copy | Download

TY  - JOUR
T1  - Application of SSR Molecular Marker Technology in Rice Variety Identification

AU  - Shasha Zhang
Y1  - 2024/12/10
PY  - 2024
N1  - https://doi.org/10.11648/j.ijaas.20241006.16
DO  - 10.11648/j.ijaas.20241006.16
T2  - International Journal of Applied Agricultural Sciences
JF  - International Journal of Applied Agricultural Sciences
JO  - International Journal of Applied Agricultural Sciences
SP  - 328
EP  - 332
PB  - Science Publishing Group
SN  - 2469-7885
UR  - https://doi.org/10.11648/j.ijaas.20241006.16
AB  - This study mainlydescribes the principles and advantages of SSR molecular marker technology, focusing on the analysis and discussion of the application of SSR molecular marker technology in rice variety identification, as well as the technical points of using SSR molecular marker technology to identify rice varieties, including the study of rapid DNA extraction methods, optimization of PCR amplification procedures and reaction systems, improvement of PCR amplification product detection methods, primer screening and construction of DNA fingerprint maps.

VL  - 10
IS  - 6
ER  -

Copy | Download