Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats

Kouam Foubi Brice Bertrand; Chuisseu Djamen Dieudonné Pascal; Dzeufiet Djomeni Paul Désiré; Samba Ngano Odette; Mouelle Sone; Tiedeu Alain Bertin; Gonsu Fotsin Joseph

doi:doi:10.11648/j.ejcbs.20160206.13

Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats

Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Mouelle Sone, Tiedeu Alain Bertin, Gonsu Fotsin Joseph

Published in European Journal of Clinical and Biomedical Sciences (Volume 2, Issue 6)

Received: 19 October 2016 Accepted: 11 November 2016 Published: 12 December 2016

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Abstract

Ionizing radiations damage cells, tissues and organs among which the liver through a cascade of molecular events that are triggered by reactive oxygen species (ROS), lipid peroxidation and nitrogen species (NS). Aged Garlic Extract (AGE) has been demonstrated to possess free radical scavenging capacity and antioxidant activity. Therefore, the present study has been focused in analyzing the properties of AGE against the lipid peroxidation and oxidative damages of rat liver tissues induced by acute radiation. Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E + Alpha Lipoïc Acid. Then, biochemical analyses, lipid peroxidation, total Protein and antioxidants assessment were made from blood samples and liver tissue homogenates. Exposure of rats to gamma radiation caused a significant increase in the level of Malondialdehyde, Nitrite, transaminase enzymes, alkaline phosphatase, and Bilirubin (Total Serum Bilirubin, Direct Bilirubin and Unconjugated Bilirubin) level while a significant decrease was recorded in serum total proteins, glutathione content, superoxide dismutase, catalase activities and total protein level. AGE treated rats revealed a significant improvement in all previous parameters. From these results, it can be concluded that AGE may have significant anti-radiation properties in rat’s liver after radiation exposure.

Published in	European Journal of Clinical and Biomedical Sciences (Volume 2, Issue 6)
DOI	10.11648/j.ejcbs.20160206.13
Page(s)	69-82
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Irradiation, Oxidative Stress, AGE, Liver, Antioxidant Enzymes, Rats

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Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Mouelle Sone, et al. (2016). Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats. European Journal of Clinical and Biomedical Sciences, 2(6), 69-82. https://doi.org/10.11648/j.ejcbs.20160206.13

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Kouam Foubi Brice Bertrand; Chuisseu Djamen Dieudonné Pascal; Dzeufiet Djomeni Paul Désiré; Samba Ngano Odette; Mouelle Sone, et al. Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats. Eur. J. Clin. Biomed. Sci. 2016, 2(6), 69-82. doi: 10.11648/j.ejcbs.20160206.13

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Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Mouelle Sone, et al. Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats. Eur J Clin Biomed Sci. 2016;2(6):69-82. doi: 10.11648/j.ejcbs.20160206.13

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@article{10.11648/j.ejcbs.20160206.13,
  author = {Kouam Foubi Brice Bertrand and Chuisseu Djamen Dieudonné Pascal and Dzeufiet Djomeni Paul Désiré and Samba Ngano Odette and Mouelle Sone and Tiedeu Alain Bertin and Gonsu Fotsin Joseph},
  title = {Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats},
  journal = {European Journal of Clinical and Biomedical Sciences},
  volume = {2},
  number = {6},
  pages = {69-82},
  doi = {10.11648/j.ejcbs.20160206.13},
  url = {https://doi.org/10.11648/j.ejcbs.20160206.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20160206.13},
  abstract = {Ionizing radiations damage cells, tissues and organs among which the liver through a cascade of molecular events that are triggered by reactive oxygen species (ROS), lipid peroxidation and nitrogen species (NS). Aged Garlic Extract (AGE) has been demonstrated to possess free radical scavenging capacity and antioxidant activity. Therefore, the present study has been focused in analyzing the properties of AGE against the lipid peroxidation and oxidative damages of rat liver tissues induced by acute radiation. Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E + Alpha Lipoïc Acid. Then, biochemical analyses, lipid peroxidation, total Protein and antioxidants assessment were made from blood samples and liver tissue homogenates. Exposure of rats to gamma radiation caused a significant increase in the level of Malondialdehyde, Nitrite, transaminase enzymes, alkaline phosphatase, and Bilirubin (Total Serum Bilirubin, Direct Bilirubin and Unconjugated Bilirubin) level while a significant decrease was recorded in serum total proteins, glutathione content, superoxide dismutase, catalase activities and total protein level. AGE treated rats revealed a significant improvement in all previous parameters. From these results, it can be concluded that AGE may have significant anti-radiation properties in rat’s liver after radiation exposure.},
 year = {2016}
}

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TY  - JOUR
T1  - Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats
AU  - Kouam Foubi Brice Bertrand
AU  - Chuisseu Djamen Dieudonné Pascal
AU  - Dzeufiet Djomeni Paul Désiré
AU  - Samba Ngano Odette
AU  - Mouelle Sone
AU  - Tiedeu Alain Bertin
AU  - Gonsu Fotsin Joseph
Y1  - 2016/12/12
PY  - 2016
N1  - https://doi.org/10.11648/j.ejcbs.20160206.13
DO  - 10.11648/j.ejcbs.20160206.13
T2  - European Journal of Clinical and Biomedical Sciences
JF  - European Journal of Clinical and Biomedical Sciences
JO  - European Journal of Clinical and Biomedical Sciences
SP  - 69
EP  - 82
PB  - Science Publishing Group
SN  - 2575-5005
UR  - https://doi.org/10.11648/j.ejcbs.20160206.13
AB  - Ionizing radiations damage cells, tissues and organs among which the liver through a cascade of molecular events that are triggered by reactive oxygen species (ROS), lipid peroxidation and nitrogen species (NS). Aged Garlic Extract (AGE) has been demonstrated to possess free radical scavenging capacity and antioxidant activity. Therefore, the present study has been focused in analyzing the properties of AGE against the lipid peroxidation and oxidative damages of rat liver tissues induced by acute radiation. Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E + Alpha Lipoïc Acid. Then, biochemical analyses, lipid peroxidation, total Protein and antioxidants assessment were made from blood samples and liver tissue homogenates. Exposure of rats to gamma radiation caused a significant increase in the level of Malondialdehyde, Nitrite, transaminase enzymes, alkaline phosphatase, and Bilirubin (Total Serum Bilirubin, Direct Bilirubin and Unconjugated Bilirubin) level while a significant decrease was recorded in serum total proteins, glutathione content, superoxide dismutase, catalase activities and total protein level. AGE treated rats revealed a significant improvement in all previous parameters. From these results, it can be concluded that AGE may have significant anti-radiation properties in rat’s liver after radiation exposure.
VL  - 2
IS  - 6
ER  -

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Author Information

Kouam Foubi Brice Bertrand

Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Chuisseu Djamen Dieudonné Pascal

Faculty of Health Sciences, Université des Montagnes, Bangangté, Bangangté, Cameroon
Dzeufiet Djomeni Paul Désiré

Faculty of Sciences, University of Yaoundé I, Yaoundé, Cameroon
Samba Ngano Odette

Faculty of Sciences, University of Dschang, Dschang, Cameroon
Mouelle Sone

Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Tiedeu Alain Bertin

National Advanced School of Engineering, University of Yaoundé I, Yaoundé, Cameroon
Gonsu Fotsin Joseph

Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon

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APA Style

Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Mouelle Sone, et al. (2016). Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats. European Journal of Clinical and Biomedical Sciences, 2(6), 69-82. https://doi.org/10.11648/j.ejcbs.20160206.13

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ACS Style

Kouam Foubi Brice Bertrand; Chuisseu Djamen Dieudonné Pascal; Dzeufiet Djomeni Paul Désiré; Samba Ngano Odette; Mouelle Sone, et al. Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats. Eur. J. Clin. Biomed. Sci. 2016, 2(6), 69-82. doi: 10.11648/j.ejcbs.20160206.13

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AMA Style

Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Mouelle Sone, et al. Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats. Eur J Clin Biomed Sci. 2016;2(6):69-82. doi: 10.11648/j.ejcbs.20160206.13

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@article{10.11648/j.ejcbs.20160206.13,
  author = {Kouam Foubi Brice Bertrand and Chuisseu Djamen Dieudonné Pascal and Dzeufiet Djomeni Paul Désiré and Samba Ngano Odette and Mouelle Sone and Tiedeu Alain Bertin and Gonsu Fotsin Joseph},
  title = {Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats},
  journal = {European Journal of Clinical and Biomedical Sciences},
  volume = {2},
  number = {6},
  pages = {69-82},
  doi = {10.11648/j.ejcbs.20160206.13},
  url = {https://doi.org/10.11648/j.ejcbs.20160206.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20160206.13},
  abstract = {Ionizing radiations damage cells, tissues and organs among which the liver through a cascade of molecular events that are triggered by reactive oxygen species (ROS), lipid peroxidation and nitrogen species (NS). Aged Garlic Extract (AGE) has been demonstrated to possess free radical scavenging capacity and antioxidant activity. Therefore, the present study has been focused in analyzing the properties of AGE against the lipid peroxidation and oxidative damages of rat liver tissues induced by acute radiation. Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E + Alpha Lipoïc Acid. Then, biochemical analyses, lipid peroxidation, total Protein and antioxidants assessment were made from blood samples and liver tissue homogenates. Exposure of rats to gamma radiation caused a significant increase in the level of Malondialdehyde, Nitrite, transaminase enzymes, alkaline phosphatase, and Bilirubin (Total Serum Bilirubin, Direct Bilirubin and Unconjugated Bilirubin) level while a significant decrease was recorded in serum total proteins, glutathione content, superoxide dismutase, catalase activities and total protein level. AGE treated rats revealed a significant improvement in all previous parameters. From these results, it can be concluded that AGE may have significant anti-radiation properties in rat’s liver after radiation exposure.},
 year = {2016}
}

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TY  - JOUR
T1  - Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats
AU  - Kouam Foubi Brice Bertrand
AU  - Chuisseu Djamen Dieudonné Pascal
AU  - Dzeufiet Djomeni Paul Désiré
AU  - Samba Ngano Odette
AU  - Mouelle Sone
AU  - Tiedeu Alain Bertin
AU  - Gonsu Fotsin Joseph
Y1  - 2016/12/12
PY  - 2016
N1  - https://doi.org/10.11648/j.ejcbs.20160206.13
DO  - 10.11648/j.ejcbs.20160206.13
T2  - European Journal of Clinical and Biomedical Sciences
JF  - European Journal of Clinical and Biomedical Sciences
JO  - European Journal of Clinical and Biomedical Sciences
SP  - 69
EP  - 82
PB  - Science Publishing Group
SN  - 2575-5005
UR  - https://doi.org/10.11648/j.ejcbs.20160206.13
AB  - Ionizing radiations damage cells, tissues and organs among which the liver through a cascade of molecular events that are triggered by reactive oxygen species (ROS), lipid peroxidation and nitrogen species (NS). Aged Garlic Extract (AGE) has been demonstrated to possess free radical scavenging capacity and antioxidant activity. Therefore, the present study has been focused in analyzing the properties of AGE against the lipid peroxidation and oxidative damages of rat liver tissues induced by acute radiation. Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E + Alpha Lipoïc Acid. Then, biochemical analyses, lipid peroxidation, total Protein and antioxidants assessment were made from blood samples and liver tissue homogenates. Exposure of rats to gamma radiation caused a significant increase in the level of Malondialdehyde, Nitrite, transaminase enzymes, alkaline phosphatase, and Bilirubin (Total Serum Bilirubin, Direct Bilirubin and Unconjugated Bilirubin) level while a significant decrease was recorded in serum total proteins, glutathione content, superoxide dismutase, catalase activities and total protein level. AGE treated rats revealed a significant improvement in all previous parameters. From these results, it can be concluded that AGE may have significant anti-radiation properties in rat’s liver after radiation exposure.
VL  - 2
IS  - 6
ER  -

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