@article{10.11648/j.ajbls.20241204.11,
  author = {Adesayo Olufunmi Olusola and Ehisdiame Favour Okoh and Oluwafemi Emmanuel Ekun and Ayodele Oluwasegun Elekan and Akindele Fakoya and Augustine Olusegun Olusola},
  title = {Antioxidant and Antiplasmodial Potentials of Methanol Bark Extract of Entada africana Via in Vitro Approaches
},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {12},
  number = {4},
  pages = {57-67},
  doi = {10.11648/j.ajbls.20241204.11},
  url = {https://doi.org/10.11648/j.ajbls.20241204.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20241204.11},
  abstract = {Plasmodium parasites, which cause malaria, continue to pose a serious threat to global health, necessitating the continuous search for novel antimalarial agents. Oxidative stress has also been linked to the pathophysiology of malaria. Entada africana is a plant known for its ethnomedicinal uses in treating various ailments associated with inflammation including malaria. This study aimed at evaluating the antiplasmodial and antioxidant potentials of methanol bark extract from Entada africana (MBEEA). In vitro approaches were adopted for the study. Plasmodium falciparum-infected erythrocyte samples were cultured in Roswell Park Memorial Institute (RPMI) 1640 media under anaerobic conditions for 72 hours. Eighteen test tubes were labeled and grouped into three replicates per group. Group I (untreated), Group II, and III were treated with chloroquine (CQ) and artemether (AR) at a concentration of 5 mg/dL. Group IV, V, and VI were treated with the extract at respective concentrations of 5 mg/dL, 10 mg/dL, and 20 mg/dL for 72 hours. The parasitemia count and the percentage parasitemia inhibition were determined by microscopic examination of Giemsa-stained smears. The antioxidant potential of the extract was assessed using in vitro assays, including superoxide radical scavenging activity (SRSA), hydroxyl radical scavenging activity (HRSA), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP). Microscopic examination of the treated samples revealed varying degrees of parasitemia inhibition. Group II and III treated with CQ and AR demonstrated a considerable reduction in parasitemia count with percentage inhibition of 100% and 83% respectively. The E. africana extract showed a concentration-dependent effect on parasitemia count. At 5 mg/dL, the extract exhibited 50% parasitemia inhibition, which increased to 100% at 10 mg/dL, and 20 mg/dL respectively. The MBEEA demonstrated significant in vitro antioxidant activities by scavenging DPPH, SRSA, and hydroxyl radical compared to the standard antioxidant (ascorbic acid). MBEEA thus exhibit potent antioxidant and antiplasmodial properties. This plant is therefore offers to be a promising medicinal plant in the treatment of malaria, hence it is recommended as potent antiplasmodial plant usable for treating malaria.
},
 year = {2024}
}

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TY  - JOUR
T1  - Antioxidant and Antiplasmodial Potentials of Methanol Bark Extract of Entada africana Via in Vitro Approaches

AU  - Adesayo Olufunmi Olusola
AU  - Ehisdiame Favour Okoh
AU  - Oluwafemi Emmanuel Ekun
AU  - Ayodele Oluwasegun Elekan
AU  - Akindele Fakoya
AU  - Augustine Olusegun Olusola
Y1  - 2024/08/30
PY  - 2024
N1  - https://doi.org/10.11648/j.ajbls.20241204.11
DO  - 10.11648/j.ajbls.20241204.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 57
EP  - 67
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20241204.11
AB  - Plasmodium parasites, which cause malaria, continue to pose a serious threat to global health, necessitating the continuous search for novel antimalarial agents. Oxidative stress has also been linked to the pathophysiology of malaria. Entada africana is a plant known for its ethnomedicinal uses in treating various ailments associated with inflammation including malaria. This study aimed at evaluating the antiplasmodial and antioxidant potentials of methanol bark extract from Entada africana (MBEEA). In vitro approaches were adopted for the study. Plasmodium falciparum-infected erythrocyte samples were cultured in Roswell Park Memorial Institute (RPMI) 1640 media under anaerobic conditions for 72 hours. Eighteen test tubes were labeled and grouped into three replicates per group. Group I (untreated), Group II, and III were treated with chloroquine (CQ) and artemether (AR) at a concentration of 5 mg/dL. Group IV, V, and VI were treated with the extract at respective concentrations of 5 mg/dL, 10 mg/dL, and 20 mg/dL for 72 hours. The parasitemia count and the percentage parasitemia inhibition were determined by microscopic examination of Giemsa-stained smears. The antioxidant potential of the extract was assessed using in vitro assays, including superoxide radical scavenging activity (SRSA), hydroxyl radical scavenging activity (HRSA), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP). Microscopic examination of the treated samples revealed varying degrees of parasitemia inhibition. Group II and III treated with CQ and AR demonstrated a considerable reduction in parasitemia count with percentage inhibition of 100% and 83% respectively. The E. africana extract showed a concentration-dependent effect on parasitemia count. At 5 mg/dL, the extract exhibited 50% parasitemia inhibition, which increased to 100% at 10 mg/dL, and 20 mg/dL respectively. The MBEEA demonstrated significant in vitro antioxidant activities by scavenging DPPH, SRSA, and hydroxyl radical compared to the standard antioxidant (ascorbic acid). MBEEA thus exhibit potent antioxidant and antiplasmodial properties. This plant is therefore offers to be a promising medicinal plant in the treatment of malaria, hence it is recommended as potent antiplasmodial plant usable for treating malaria.

VL  - 12
IS  - 4
ER  - 

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