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Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis

Received: 14 September 2019     Accepted: 29 October 2019     Published: 13 November 2019
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Abstract

This study was performed to study the effects of astragalus polysaccharides (APS) in the treatment of ulcerative colitis (UC), and to explore whether myeloid-derived suppressor cells (MDSC) involve in this process. UC model was established by dextran sulfate sodium salt (DSS) inducement in mouse. Then the effects of APS on UC was evaluate at the levels of cytology and molecular biology: exploring the influence of APS on MDSC by analyzing the levels of MDSC before and after the treatments of APS; evaluating the therapeutic effects of APS on UC by pathologic histology. Data showed that the levels of MDSC in bone marrow, spleen, peripheral blood of UC mice were significantly decreased after intervention with APS, indicating APS inhibited the level of MDSC significantly. After depletion bone marrow cells in mice, we further found that the therapeutic effects of APS in MDSC- group was significantly reduced compared with MDSC+ group. It was discovered through pathological analysis that, compared with UC model group, APS intervention group mainly manifested as reduced infiltrating acute and chronic inflammatory cells, necrotic epithelial cells and epithelial ulceration. Therefore, APS could reduce the inflammatory cell infiltration in colonic tissues of UC model mice, repair the damaged colonic mucosa, and promote ulcer healing. In conclusion, APS has a potential application in the treatment of ulcerative colitis, and is dependent or partially dependent on MDSC to achieve this effects.

Published in American Journal of Biomedical and Life Sciences (Volume 7, Issue 6)

This article belongs to the Special Issue Inflammatory Bowel Disease

DOI 10.11648/j.ajbls.20190706.13
Page(s) 143-147
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Astragalus Polysaccharides, Ulcerative Colitis, Mouse Model, MDSC

References
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[3] Ni J, Wu G, Albenberg L, Tomov V. Gut microbiota and IBD: causation or correlation? NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY. 2017; 14 (10): 573-84.
[4] Hao WB, Xiang FF, Liu QL, et al. Research progress of myeloid-derived suppressor cells in tumor microenvironment [J]. Journal of Immunology, 2017, 33 (8): 729-732.
[5] Yang M, Qian XH, Zhao DH, et al. Effects of Astragalus polysaccharides to the erythroid lineage and microarray analysis in K562 cells [J]. Ethnopharmacol, 2010, 127 (2): 242-251.
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[7] Yang WB, Chen QL, Ma LY, et al. Effect of astragalus polysaccharides on rabbit atherosclerotic endothelial cell function [J]. Shaanxi Medical Journal, 2005, 34 (8): 914-918.
[8] Xia Y, Qin ZR, Shi YM. Therapeutic effect observation on 30 cases of ulcerative colitis treated with internal and external treatment [J]. Shanghai Journal of Traditional Chinese Medicine, 2000, 46 (10): 24.
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[11] Han W, Liu ZQ, Sun L. Clinical research of using Salvia miltiorrhiza and astragalus injection in treating ulcerative colitis [J]. Journal of Changchun College of Traditional Chinese Medicine, 2005, 21 (4): 7.
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[13] Han JC, Yu YS, Tang ZH, et al. Effect of astragalus polysaccharides on the differentiation and maturation of mouse dendritic cells [J]. Shanghai Medicine, 2006, 29 (12): 873-876.
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[15] Chen W, Li YM, Yu MH, et al. Immune regulatory effect of astragalus polysaccharides on the T-cell subset in diabetic mice [J]. China Journal of Modern Medicine, 2007, 17 (1): 28-31.
[16] Zhao LH, Ma ZX, Zhu J, et al. Characterization of polysaccharides from astragalus radix as the macrophage stimulator [J]. Cellular Immunol, 2011, 271 (2): 329-334.
[17] Zang KH, LI YD, ZHU LJ, et al. Study on the repair effect on colon mucosa and mechanism of astragalus polysaccharides in rats with ulcerative colitis [J]. GANSU UNIVERSITY OF CHINESE MEDICINE, 2018, 035 (003): P. 5-10.
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    Xi Qinhua, Teng Yajie, Li Yueqin, Dai Juan, Zhang Guangbo, et al. (2019). Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis. American Journal of Biomedical and Life Sciences, 7(6), 143-147. https://doi.org/10.11648/j.ajbls.20190706.13

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    ACS Style

    Xi Qinhua; Teng Yajie; Li Yueqin; Dai Juan; Zhang Guangbo, et al. Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis. Am. J. Biomed. Life Sci. 2019, 7(6), 143-147. doi: 10.11648/j.ajbls.20190706.13

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    AMA Style

    Xi Qinhua, Teng Yajie, Li Yueqin, Dai Juan, Zhang Guangbo, et al. Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis. Am J Biomed Life Sci. 2019;7(6):143-147. doi: 10.11648/j.ajbls.20190706.13

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  • @article{10.11648/j.ajbls.20190706.13,
      author = {Xi Qinhua and Teng Yajie and Li Yueqin and Dai Juan and Zhang Guangbo and Chen Weichang},
      title = {Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {7},
      number = {6},
      pages = {143-147},
      doi = {10.11648/j.ajbls.20190706.13},
      url = {https://doi.org/10.11648/j.ajbls.20190706.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20190706.13},
      abstract = {This study was performed to study the effects of astragalus polysaccharides (APS) in the treatment of ulcerative colitis (UC), and to explore whether myeloid-derived suppressor cells (MDSC) involve in this process. UC model was established by dextran sulfate sodium salt (DSS) inducement in mouse. Then the effects of APS on UC was evaluate at the levels of cytology and molecular biology: exploring the influence of APS on MDSC by analyzing the levels of MDSC before and after the treatments of APS; evaluating the therapeutic effects of APS on UC by pathologic histology. Data showed that the levels of MDSC in bone marrow, spleen, peripheral blood of UC mice were significantly decreased after intervention with APS, indicating APS inhibited the level of MDSC significantly. After depletion bone marrow cells in mice, we further found that the therapeutic effects of APS in MDSC- group was significantly reduced compared with MDSC+ group. It was discovered through pathological analysis that, compared with UC model group, APS intervention group mainly manifested as reduced infiltrating acute and chronic inflammatory cells, necrotic epithelial cells and epithelial ulceration. Therefore, APS could reduce the inflammatory cell infiltration in colonic tissues of UC model mice, repair the damaged colonic mucosa, and promote ulcer healing. In conclusion, APS has a potential application in the treatment of ulcerative colitis, and is dependent or partially dependent on MDSC to achieve this effects.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis
    AU  - Xi Qinhua
    AU  - Teng Yajie
    AU  - Li Yueqin
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    DO  - 10.11648/j.ajbls.20190706.13
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 143
    EP  - 147
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20190706.13
    AB  - This study was performed to study the effects of astragalus polysaccharides (APS) in the treatment of ulcerative colitis (UC), and to explore whether myeloid-derived suppressor cells (MDSC) involve in this process. UC model was established by dextran sulfate sodium salt (DSS) inducement in mouse. Then the effects of APS on UC was evaluate at the levels of cytology and molecular biology: exploring the influence of APS on MDSC by analyzing the levels of MDSC before and after the treatments of APS; evaluating the therapeutic effects of APS on UC by pathologic histology. Data showed that the levels of MDSC in bone marrow, spleen, peripheral blood of UC mice were significantly decreased after intervention with APS, indicating APS inhibited the level of MDSC significantly. After depletion bone marrow cells in mice, we further found that the therapeutic effects of APS in MDSC- group was significantly reduced compared with MDSC+ group. It was discovered through pathological analysis that, compared with UC model group, APS intervention group mainly manifested as reduced infiltrating acute and chronic inflammatory cells, necrotic epithelial cells and epithelial ulceration. Therefore, APS could reduce the inflammatory cell infiltration in colonic tissues of UC model mice, repair the damaged colonic mucosa, and promote ulcer healing. In conclusion, APS has a potential application in the treatment of ulcerative colitis, and is dependent or partially dependent on MDSC to achieve this effects.
    VL  - 7
    IS  - 6
    ER  - 

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Author Information
  • Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China

  • Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China

  • Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China

  • Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China

  • Institute of Clinical Immunology, First Affiliated Hospital of Soochow University, Suzhou, China

  • Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China

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