| Peer-Reviewed

Evaluation of the Impact of the Trivedi Effect® -Energy of Consciousness on the Structure and Isotopic Abundance Ratio of Magnesium Gluconate Using LC-MS and NMR Spectroscopy

Received: 7 February 2017     Accepted: 18 February 2017     Published: 9 March 2017
Views:       Downloads:
Abstract

Magnesium gluconate is a classical pharmaceutical/nutraceutical compound used as a magnesium ion source for the prevention and treatment of hypomagnesemia. The present study was aimed to investigate the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of both the control and treated samples indicated the presence of mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min and fragmentation pattern of the both sample were almost similar. The relative peak intensities of the fragment ions were significantly changed in the treated sample compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were observed almost similar for the control and the treated samples. The percentage change in the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly decreased in the treated sample by 17.51% compared with the control sample. Consequently, the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) in the treated sample was significantly increased by 79.44% compared to the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in treated sample were significantly altered compared with the control sample. Thus, The Trivedi Effect® Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects. Consequently, The Trivedi Effect® Treated magnesium gluconate would be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections.

Published in American Journal of Biomedical and Life Sciences (Volume 5, Issue 1)
DOI 10.11648/j.ajbls.20170501.12
Page(s) 6-15
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Magnesium Gluconate, Biofield Energy Healing Treatment, Consciousness Energy Healing Treatment, Biofield Energy Healers, The Trivedi Effect®, LC-MS, NMR, Isotopic Abundance Ratio, Isotope Effect

References
[1] Ramachandran S, Fontanille P, Pandey A, Larroche C (2006) Gluconic acid: Properties, applications and microbial production. Food Technol Biotechnol 44. 185-195.
[2] Heaton FW (1990) Role of magnesium in enzyme systems in metal ions in biological systems, In: Sigel H, Sigel A (Eds.), Volume 26. Compendium on magnesium and its role in biology, nutrition and physiology, Marcel Dekker Inc., New York.
[3] Garfinkel L, Garfinkel D (1985) Magnesium regulation of the glycolytic pathway and the enzymes involved. Magnesium 4: 60-72.
[4] Swaminathan R (2003) Magnesium metabolism and its disorders. Clin Biochem Rev 24: 47-66.
[5] Gröber U, Schmidt J, Kisters K (2015) Magnesium in prevention and therapy. Nutrients 7: 8199-8226.
[6] William JH, Danziger J (2016) Magnesium deficiency and proton-pump inhibitor use: A clinical review. J Clin Pharmacol 56. 660-668.
[7] Guerrera MP, Volpe SL, Mao JJ (2009) Therapeutic uses of magnesium. Am Fam Physician 80: 157-162.
[8] Coudray C, Rambeau M, Feillet-Coudray C, Gueux E, Tressol JC, Mazur A, Rayssiguier Y (2005) Study of magnesium bioavailability from ten organic and inorganic Mg salts in Mg-depleted rats using a stable isotope approach. Magnes Res 18: 215-223.
[9] Fleming TE, Mansmann Jr HC (1999) Methods and compositions for the prevention and treatment of diabetes mellitus. United States Patent 5871769, 1-10.
[10] Fleming TE, Mansmann Jr HC (1999) Methods and compositions for the prevention and treatment of immunological disorders, inflammatory diseases and infections. United States Patent 5939394, 1-11.
[11] Weglicki WB (2000) Intravenous magnesium gluconate for treatment of conditions caused by excessive oxidative stress due to free radical distribution. United States Patent 6100297, 1-6.
[12] Turner RJ, Dasilva KW, O'Connor C, van den Heuvel C, Vink R (2004) Magnesium gluconate offers no more protection than magnesium sulphate following diffuse trau-matic braininjury in rats. J Am Coll Nutr 23: 541S-544S.
[13] Martin RW, Martin JN Jr, Pryor JA, Gaddy DK, Wiser WL, Morrison JC (1988) Comparison of oral ritodrine and magnesium gluconate for ambulatory tocolysis. Am J Obstet Gynecol 158: 1440-1445.
[14] Lee KH, Chung SH, Song JH, Yoon JS, Lee J, Jung MJ, Kim JH (2013) Cosmetic compositions for skin-tightening and method of skin-tightening using the same. United States Patent 8580741 B2.
[15] Stenger VJ (1999) Bioenergetic fields. Sci Rev Alternative Med 3.
[16] Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita 35. 22-29.
[17] Hammerschlag R, Jain S, Baldwin AL, Gronowicz G, Lutgendor SK, Oschman JL, Yount GL (2012) Biofield research: A roundtable discussion of scientific and methodological issues. J Altern Complement Med 18: 1081-1086.
[18] Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.
[19] Koithan M (2009) Introducing complementary and alternative therapies. J Nurse Pract 5: 18-20.
[20] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Antibiogram, biochemical reactions and biotyping of biofield treated Providencia rettgeri. American Journal of Health Research 3: 344-351.
[21] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Phenotypic and biotypic characterization of Klebsiella oxytoca: An impact of biofield treatment. J Microb Biochem Technol 7. 202-205.
[22] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7. 253-257.
[23] Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Analysis of genetic diversity using simple sequence repeat (SSR) markers and growth regulator response in biofield treated cotton (Gossypium hirsutum L.). American Journal of Agriculture and Forestry 3: 216-221.
[24] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of biochemical marker - glutathione and DNA fingerprinting of biofield energy treated Oryza sativa. American Journal of BioScience 3. 243-248.
[25] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Effect of biofield treated energized water on the growth and health status in chicken (Gallus gallus domesticus). Poult Fish Wildl Sci 3. 140.
[26] Trivedi MK, Branton A, Trivedi D, Nayak G, Singh R, Jana S (2015) Physicochemical and spectroscopic characterization of biofield energy treated gerbera multiplication medium. Plant 3: 57-63.
[27] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Jana S, Mishra R (2015) Evaluation of the impact of biofield treatment on physical and thermal properties of casein enzyme hydrolysate and casein yeast peptone. Clin Pharmacol Biopharm 4: 138.
[28] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Evaluation of biofield treatment on physical, atomic and structural characteristics of manganese (II, III) oxide. J Material Sci Eng 4. 177.
[29] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Physicochemical and atomic characterization of silver powder after biofield treatment. J Bioengineer & Biomedical Sci 5. 165.
[30] Trivedi MK, Patil S, Shettigar H, Singh R, Jana S (2015) An impact of biofield treatment on spectroscopic characterization of pharmaceutical compounds. Mod Chem Appl 3. 159.
[31] Trivedi MK, Branton A, Trivedi D, Nayak G, Singh R, Jana S (2015) Characterisation of physical, spectral and thermal properties of biofield treated resorcinol. Organic Chem Curr Res 4: 146.
[32] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Evaluation of biofield energy treatment on physical and thermal characteristics of selenium powder. Journal of Food and Nutrition Sciences.3: 223-228.
[33] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Jana S, Mishra RK (2015) Bio-field treatment: An effective strategy to improve the quality of beef extract and meat infusion powder. J Nutr Food Sci 5. 389.
[34] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mishra RK, Jana S (2015) Biofield energy treatment: A potential strategy for modulating physical, thermal and spectral properties of 3-chloro-4-fluoroaniline. J Thermodyn Catal 6. 151.
[35] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mishra RK, Jana S (2015) Physical, thermal and spectral properties of biofield treated 3-nitroacetophenone, Science Journal of Analytical Chemistry 3: 71-79.
[36] Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2016) Determination of isotopic abundance of 2H, 13C, 18O, and 37Cl in biofield energy treated dichlorophenol isomers. Science Journal of Analytical Chemistry 4: 1-6.
[37] Trivedi MK, Branton A, Trivedi D, Nayak G, Sethi KK, Jana S (2016) Determination of isotopic abundance ratio of biofield energy treated 1, 4-dichlorobenzene using gas chromatography-mass spectrometry (GC-MS). Modern Chemistry 4: 30-37.
[38] Trivedi MK, Branton A, Trivedi D, Nayak G, Panda P, Jana S (2016) Isotopic abundance ratio analysis of 1, 2, 3-trimethoxybenzene (TMB) after biofield energy treatment (The Trivedi Effect®) using gas chromatography-mass spectrometry, American Journal of Applied Chemistry 4: 132-140.
[39] Trivedi MK, Branton A, Trivedi D, Nayak G, Sethi KK, Jana S (2016) Evaluation of isotopic abundance ratio in biofield energy treated nitrophenol derivatives using gas chromatography-mass spectrometry. American Journal of Chemical Engineering 4. 68-77.
[40] Schellekens RC, Stellaard F, Woerdenbag HJ, Frijlink HW, Kosterink JG (2011) Applications of stable isotopes in clinical pharmacology. Br J Clin Pharmacol 72. 879-897.
[41] Muccio Z, Jackson GP (2009) Isotope ratio mass spectrometry. Analyst 134: 213-222.
[42] Vanhaecke F, Kyser K (2012) Isotopic composition of the elements In Isotopic Analysis: Fundamentals and applications using ICP-MS (1stedn), Edited by Vanhaecke F, Degryse P. Wiley-VCH GmbH & Co. KGaA, Weinheim.
[43] Smith RM (2004) Understanding Mass Spectra: A Basic Approach, Second Edition, John Wiley & Sons, Inc, ISBN 0-471-42949-X.
[44] Meija J, Coplen TB, Berglund M, Brand WA, De Bievre P, Groning M, Holden NE, Irrgeher J, Loss RD, Walczyk T, Prohaska T (2016) Isotopic compositions of the elements 2013 (IUPAC technical Report). Pure Appl Chem 88: 293-306.
[45] Asperger S (2003) Chemical Kinetics and Inorganic Reaction Mechanisms Springer science + Business media, New York.
[46] Trivedi MK, Mohan TRR (2016) Biofield energy signals, energy transmission and neutrinos. American Journal of Modern Physics 5: 172-176.
[47] Cleland WW (2003) The use of isotope effects to determine enzyme mechanisms. J Biol Chem 278: 51975-51984.
[48] Nikolic VD, Illic DP, Nikolic LB, Stanojevic LP, Cakic MD, Tacic AD, Ilic-Stojanovic SS (2014) The synthesis and characterization of iron (II) gluconate. Advanced technologies 3: 16-24.
Cite This Article
  • APA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Michael Peter Ellis, et al. (2017). Evaluation of the Impact of the Trivedi Effect® -Energy of Consciousness on the Structure and Isotopic Abundance Ratio of Magnesium Gluconate Using LC-MS and NMR Spectroscopy. American Journal of Biomedical and Life Sciences, 5(1), 6-15. https://doi.org/10.11648/j.ajbls.20170501.12

    Copy | Download

    ACS Style

    Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Michael Peter Ellis, et al. Evaluation of the Impact of the Trivedi Effect® -Energy of Consciousness on the Structure and Isotopic Abundance Ratio of Magnesium Gluconate Using LC-MS and NMR Spectroscopy. Am. J. Biomed. Life Sci. 2017, 5(1), 6-15. doi: 10.11648/j.ajbls.20170501.12

    Copy | Download

    AMA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Michael Peter Ellis, et al. Evaluation of the Impact of the Trivedi Effect® -Energy of Consciousness on the Structure and Isotopic Abundance Ratio of Magnesium Gluconate Using LC-MS and NMR Spectroscopy. Am J Biomed Life Sci. 2017;5(1):6-15. doi: 10.11648/j.ajbls.20170501.12

    Copy | Download

  • @article{10.11648/j.ajbls.20170501.12,
      author = {Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Michael Peter Ellis and James Jeffery Peoples and James Joseph Meuer and Johanne Dodon and John Lawrence Griffin and John Suzuki and Joseph Michael Foty and Judy Weber and Julia Grace McCammon and Karen Brynes Allen and Kathryn Regina Sweas and Lezley Jo-Anne Wright and Lisa A. Knoll and Madeline E. Michaels and Margaret Kweya Wahl and Mark E. Stutheit and Michelle Barnard and Muriel Mae Ranger and Paromvong Sinbandhit and V. J. Kris Elig and Kalyan Kumar Sethi and Parthasarathi Panda and Snehasis Jana},
      title = {Evaluation of the Impact of the Trivedi Effect® -Energy of Consciousness on the Structure and Isotopic Abundance Ratio of Magnesium Gluconate Using LC-MS and NMR Spectroscopy},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {5},
      number = {1},
      pages = {6-15},
      doi = {10.11648/j.ajbls.20170501.12},
      url = {https://doi.org/10.11648/j.ajbls.20170501.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20170501.12},
      abstract = {Magnesium gluconate is a classical pharmaceutical/nutraceutical compound used as a magnesium ion source for the prevention and treatment of hypomagnesemia. The present study was aimed to investigate the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of both the control and treated samples indicated the presence of mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min and fragmentation pattern of the both sample were almost similar. The relative peak intensities of the fragment ions were significantly changed in the treated sample compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were observed almost similar for the control and the treated samples. The percentage change in the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly decreased in the treated sample by 17.51% compared with the control sample. Consequently, the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) in the treated sample was significantly increased by 79.44% compared to the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in treated sample were significantly altered compared with the control sample. Thus, The Trivedi Effect® Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects. Consequently, The Trivedi Effect® Treated magnesium gluconate would be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections.},
     year = {2017}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Evaluation of the Impact of the Trivedi Effect® -Energy of Consciousness on the Structure and Isotopic Abundance Ratio of Magnesium Gluconate Using LC-MS and NMR Spectroscopy
    AU  - Mahendra Kumar Trivedi
    AU  - Alice Branton
    AU  - Dahryn Trivedi
    AU  - Gopal Nayak
    AU  - Michael Peter Ellis
    AU  - James Jeffery Peoples
    AU  - James Joseph Meuer
    AU  - Johanne Dodon
    AU  - John Lawrence Griffin
    AU  - John Suzuki
    AU  - Joseph Michael Foty
    AU  - Judy Weber
    AU  - Julia Grace McCammon
    AU  - Karen Brynes Allen
    AU  - Kathryn Regina Sweas
    AU  - Lezley Jo-Anne Wright
    AU  - Lisa A. Knoll
    AU  - Madeline E. Michaels
    AU  - Margaret Kweya Wahl
    AU  - Mark E. Stutheit
    AU  - Michelle Barnard
    AU  - Muriel Mae Ranger
    AU  - Paromvong Sinbandhit
    AU  - V. J. Kris Elig
    AU  - Kalyan Kumar Sethi
    AU  - Parthasarathi Panda
    AU  - Snehasis Jana
    Y1  - 2017/03/09
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajbls.20170501.12
    DO  - 10.11648/j.ajbls.20170501.12
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 6
    EP  - 15
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20170501.12
    AB  - Magnesium gluconate is a classical pharmaceutical/nutraceutical compound used as a magnesium ion source for the prevention and treatment of hypomagnesemia. The present study was aimed to investigate the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of both the control and treated samples indicated the presence of mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.52 min and fragmentation pattern of the both sample were almost similar. The relative peak intensities of the fragment ions were significantly changed in the treated sample compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were observed almost similar for the control and the treated samples. The percentage change in the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly decreased in the treated sample by 17.51% compared with the control sample. Consequently, the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) in the treated sample was significantly increased by 79.44% compared to the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in treated sample were significantly altered compared with the control sample. Thus, The Trivedi Effect® Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects. Consequently, The Trivedi Effect® Treated magnesium gluconate would be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections.
    VL  - 5
    IS  - 1
    ER  - 

    Copy | Download

Author Information
  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Sections