Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark

James; Dorcas Bolanle; Sheneni; Victor Duniya; Kadejo; Olubukola Adetoro; Yatai; Kenneth Bobai

doi:doi:10.11648/j.ajbls.20140201.14

| Peer-Reviewed

Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark

James, Dorcas Bolanle, Sheneni, Victor Duniya, Kadejo, Olubukola Adetoro, Yatai, Kenneth Bobai

Published in American Journal of Biomedical and Life Sciences (Volume 2, Issue 1)

Received: 20 January 2014 Published: 20 March 2014

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Phytochemical screening and antioxidant activities in different solvent extracts of Vitex doniana leaves, stem bark and root bark was carried out. The extracts were subjected to various chemical test for phytochemical constituents, total phenolic contents were evaluated using Folin Ciocalteu method and their antioxidant activity was assayed through in- vitro radical scavenging activity using DPPH• assay, phytochemical screening of this study indicate the presence of cardiac glycosides, tannins, flavonoids, saponnins, alkaloids, steroids and terpenoids. The average total phenol content of leaves methanol and leaves ethanol extracts were significantly (P<0.05) higher when compared with the total polyphenol contents in the acetone and aqueous solvent extracts. In order of effectiveness (IC50) of the plant extracts the potent inhibitors was leaves ethanol extract (0.227mg/ml), follow by stem bark ethanol extract (0.236mg/ml), the least was the root ethanol extract (0.567mg/ml).This shows that Vitex doniana solvent extracts especially the ethanolic leaves extracts may be a potent source of natural antioxidant and it use in the management of diseases associated with oxidative stress is justify.

Published in	American Journal of Biomedical and Life Sciences (Volume 2, Issue 1)
DOI	10.11648/j.ajbls.20140201.14
Page(s)	22-27
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Phytochemical, Antioxidant, Vitex Doniana, DPPH, Solvent Extraction

References

[1]	Manjula E, Mamidala, E. An ethnobotanical survey of medicinal plants used by traditional healers of Thadvai, Warangal district, Andhra Pradesh, India. IJMRHS. 2013; 2(1): 40-46.
[2]	Halliwell B, Gutteridge JMC. Free radicals in biology and medicine, 3rd Edn London: Oxford University Press New York; 1999,p 246-350
[3]	Mantle D, Eddeb F, Pickering AT. Comparison of relative antioxidant activities of British medicinal plant species in vitro. J Ethnopharmacol 2000; 72: 47–51.
[4]	Sharma V, Paliwal R, Pracheta, Sharma S. Phytochemical analysis and evaluation of antioxidant activities of hydro-ethanolic extracts of Moringa oleifera lam pods. J Pharm Res. 2011; 4(2): 554-7.
[5]	Kostic DA , Velickovic JM, Mitic SS, Mitic MN, Randjelovic SS, Arsic BB et al. Correlation among phenolic , toxic metals and antioxidant activity of the extracts of plant species from southeast Serbia. Bull. Chem. Soc. Ethop. 2013; 27(2)169-178
[6]	Sarang Jain, Ameeta Argal. Evaluation of antioxidant activity of a polyherbal formulation. Asian J Pharm Clin Res. 2012; 5(2):194-195
[7]	Mohammedi Z, Atik, F. Impact of solvent extraction type on total polyphenols content and biological activity from Tamarix aphylla (L.) Karst, , IJPBS. 2011; 2: 609-615
[8]	Dariusz Kowalczyk, Michał Świeca, Joanna Cichocka, Urszula Gawlik-Dziki. The phenolic content and antioxidant activity of the aqueous and hydroalcoholic extracts of hops and their pellets. J Inst Brew.2013; 119(3):103-110
[9]	Balakrishnan N, Mayank Srivastava, Pallavi Tiwari. Preliminary Phytochemical Analysis and DPPH Free Radical Scavenging Activity of Trewia nudiflora Linn. Roots and Leaves. Pak. J. Biol. Sci. 2013; 16: 1403-1406
[10]	Kowalczyk, D., Świeca, M., Cichocka, J, Gawlik-Dziki, U. The phenolic content and antioxidant activity of the aqueous and hydroalcoholic extracts of hops and their pellets. J Inst Brew. 2013; 119: 103–110. doi: 10.1002/jib.73
[11]	Aupam Ghosh, Nandita Chowdhury , Goutam Chandra. Plant extracts as potential moisquito larvacides. Indian J med Res. 2012; 135:581-598
[12]	Sofowora A. Medical plant and Traditional Medicine in Africa, 1st Edn., John Wiley &Son Ltd New York, Chichester, New York, 1982 ISBN-10 p 256.
[13]	Trease and Evans WC. Text book of pharmacognosy 12th ed. BacillareTrindal; Estonbol London, U.K; 1993, p 1-59
[14]	Amin I, Zamaliah MM, Chin WF. Total antioxidant activity and phenolic content in selected vegetables. Food Chem 2004; 87(4): 581- 586
[15]	Chan EWC, Lim YY, Omar M. Antioxidant and antibacterial activity of leaves of Etlingera species (Zingiberaceae) in Peninsular Malaysia. Food Chem 2007; 104(4): 1586-1593.
[16]	Chukwuka KS, Ikheloa JO, Okonko IO, Moody JO, Mankinde TA. The antimicrobial activities of some medicinal plants on Escherichia colias an agent of diarrhoea in livestock. Advan. Appl. Sci.Res. 2011; 2: 37-48
[17]	Jayaprakasha GK, Singh RP, Sakariah KK. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem. 2001; 73: 285-290.
[18]	Saeedeh AD, Urooj A. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem 2007; 102: 1233-1240.
[19]	Quang-Vinh Nguyen, Jong-Bang Eun. Antioxidant activity of solvent extracts from Vietnamese medicinal plants. JMPR 2011; 5(13), 2798-2811.
[20]	Kasote D.M. Flaxseed phenolics as natural antioxidants. Int. food res. J 2013; 20(1):27-34.
[21]	Chaouche TM, Haddouchi F, Ksouri R, Medini F, EL-Haci IA, Boucherit Z, et al. Antioxidant Potential of Hydro-methanolic Extract of Prasium majus L: An in vitro Study. Pak. J. Biol. Sci.2013; 16: 1318-1323.
[22]	Gopakumar Gopinathan Nair, Cherupally Krishnan, Krishnan Nair, "Radioprotective Effects of Gallic Acid in Mice." Biomed Res Int. Article ID 953079, 2013 vol 2013,:13 pages. doi:10.1155/2013/953079
[23]	Zohra Mohammedi. Impact of solvent extraction type on total polyphenols content and biological actalivity from Tamarix aphylla (L). Int. J. Pharm. Biol. 2011; 2(1): 609-615
[24]	Norshazila S, Syed Zahir I, Mustapha Suleiman K, Aisyah MR, Kamarul Rahim K Antioxidant Levels and Activities of Selected Seeds of Malaysian Tropical Fruits. Mal J Nutr. 2010; 16(1): 149-159.
[25]	José Teixeira, Alexandra Gaspar, E. Manuela Garrido, Jorge Garrido, and Fernanda Borges, "Hydroxycinnamic Acid Antioxidants: An Electrochemical Overview," Biomed Res. Int. Article ID 251754, vol. 2013, 11 pages, 2013. doi:10.1155/2013/251754
[26]	Sabina Parajuli, Nirmala Tilija Pun, Shraddha Parajuli, Nirmala Jamarattel-Pandit. Antioxidant activity, total polyphenol and Flavonoid contents in some selected medicinal plant of Nepal. JHAS. 2012; 2(1):27-31.
[27]	Muhammad Athar Abbasi, Hina Saleeem,Aziz-ur-Rehman, Tauheeda Riaz and Muhammad Ajaib.Determination of Antioxidant Activity and phytoconstituent screening of Euphorbia heterophylla linn, Br. J. Pharm. Res. 2013; 3(2):202-216
[28]	Pattanayak SP, Mitra Mazumder P, Sunita P. Total Phenolic Content, Flavonoid Content and in vitro Antioxidant Activities of Dendrophthoe falcata (L.f.) Ettingsh. Res J Med Plant. 2012; 6: 136-148.
[29]	Norshazila S, Syed Zahir I, Mustapha Suleiman K, Aisyah MR, Kamarul Rahim K Antioxidant Levels and Activities of Selected Seeds of Malaysian Tropical Fruits. Mal J Nutr. 2010; 16(1): 149-159.
[30]	Noriham Abdullah, Wan Saidatul Syida ,Wan Kamarudin, Zainal Samicho, Khairusy Syakirah Zulkifli, Nurain Aziman. Study on Antioxidant Capacity and Phenolic Content of Various Parts of Wax Gourd (Benincasa hispida). World Appl. Sci. J.2012; 19 (7): 1051-1056.
[31]	Barku VYA, Opoku-Boahen Y, Owosu-Ansah E, Mensah EF. Antioxidant activity and the the estimation of total polyphenolic and flavonoid contents of the root extract of Amaranthus spinosus. Asian J. Plant sci. res. 2013; 3(1):69-74
[32]	Shaida Fariza Sulaiman , Nor Adlin Md. Yusoff, Ibrahim M. Eldeen, Eng Meng Seow, Azliana Abu Bakar Sajak, Supriatno Kheng Leong Ooi Correlation between total phenolic and mineral contents with antioxidant activity of eight Malaysian bananas (Musa sp.) J Food Compos Analy. 2011; 24: 1–10.
[33]	Chinedu Prosper Anokwuru, Ijeoma Esiaba, Olusola Ajibaye and Ayobami O. Adesuyi. Polyphenolic Content and Antioxidant Activity of Hibiscus sabdariffa Calyx. Res. J.Med. Plant. 2011; 5: 557-566.
[34]	Wen Jie Li, Xian Long Cheng, Jing Liu, Rui Chao Lin, Gang Li Wang, Shu Shan Du et al. Phenolic Compounds and antioxidant activities of Liriope muscari. Molecules. 2012; 17: 1797-1808.

Cite This Article

Plain Text BibTeX RIS

APA Style

James, Dorcas Bolanle, Sheneni, Victor Duniya, Kadejo, et al. (2014). Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark. American Journal of Biomedical and Life Sciences, 2(1), 22-27. https://doi.org/10.11648/j.ajbls.20140201.14

Copy | Download

ACS Style

James; Dorcas Bolanle; Sheneni; Victor Duniya; Kadejo, et al. Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark. Am. J. Biomed. Life Sci. 2014, 2(1), 22-27. doi: 10.11648/j.ajbls.20140201.14

Copy | Download

AMA Style

James, Dorcas Bolanle, Sheneni, Victor Duniya, Kadejo, et al. Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark. Am J Biomed Life Sci. 2014;2(1):22-27. doi: 10.11648/j.ajbls.20140201.14

Copy | Download

@article{10.11648/j.ajbls.20140201.14,
  author = {James and Dorcas Bolanle and Sheneni and Victor Duniya and Kadejo and Olubukola Adetoro and Yatai and Kenneth Bobai},
  title = {Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {2},
  number = {1},
  pages = {22-27},
  doi = {10.11648/j.ajbls.20140201.14},
  url = {https://doi.org/10.11648/j.ajbls.20140201.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140201.14},
  abstract = {Phytochemical screening and antioxidant activities in different solvent extracts of Vitex doniana leaves, stem bark and root bark was carried out. The extracts were subjected  to various chemical test for phytochemical constituents, total phenolic contents were evaluated using Folin Ciocalteu method and their antioxidant activity was assayed through in- vitro radical scavenging activity using DPPH• assay, phytochemical screening of this study indicate the presence of cardiac glycosides, tannins, flavonoids, saponnins, alkaloids, steroids and terpenoids. The average total phenol content of leaves methanol and leaves ethanol extracts were significantly (P<0.05) higher when compared with the total polyphenol contents in the acetone and aqueous solvent extracts. In order of effectiveness (IC50) of the plant extracts the potent inhibitors was leaves ethanol extract (0.227mg/ml), follow by stem bark ethanol extract (0.236mg/ml), the least was the root ethanol extract (0.567mg/ml).This shows that Vitex doniana solvent extracts especially the ethanolic leaves extracts may be a potent source of natural antioxidant and it use in the management of diseases associated with oxidative stress is justify.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark
AU  - James
AU  - Dorcas Bolanle
AU  - Sheneni
AU  - Victor Duniya
AU  - Kadejo
AU  - Olubukola Adetoro
AU  - Yatai
AU  - Kenneth Bobai
Y1  - 2014/03/20
PY  - 2014
N1  - https://doi.org/10.11648/j.ajbls.20140201.14
DO  - 10.11648/j.ajbls.20140201.14
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 22
EP  - 27
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20140201.14
AB  - Phytochemical screening and antioxidant activities in different solvent extracts of Vitex doniana leaves, stem bark and root bark was carried out. The extracts were subjected  to various chemical test for phytochemical constituents, total phenolic contents were evaluated using Folin Ciocalteu method and their antioxidant activity was assayed through in- vitro radical scavenging activity using DPPH• assay, phytochemical screening of this study indicate the presence of cardiac glycosides, tannins, flavonoids, saponnins, alkaloids, steroids and terpenoids. The average total phenol content of leaves methanol and leaves ethanol extracts were significantly (P<0.05) higher when compared with the total polyphenol contents in the acetone and aqueous solvent extracts. In order of effectiveness (IC50) of the plant extracts the potent inhibitors was leaves ethanol extract (0.227mg/ml), follow by stem bark ethanol extract (0.236mg/ml), the least was the root ethanol extract (0.567mg/ml).This shows that Vitex doniana solvent extracts especially the ethanolic leaves extracts may be a potent source of natural antioxidant and it use in the management of diseases associated with oxidative stress is justify.
VL  - 2
IS  - 1
ER  -

Copy | Download

Author Information

James
Dorcas Bolanle
Sheneni
Victor Duniya
Kadejo
Olubukola Adetoro
Yatai
Kenneth Bobai

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

James, Dorcas Bolanle, Sheneni, Victor Duniya, Kadejo, et al. (2014). Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark. American Journal of Biomedical and Life Sciences, 2(1), 22-27. https://doi.org/10.11648/j.ajbls.20140201.14

Copy | Download

ACS Style

James; Dorcas Bolanle; Sheneni; Victor Duniya; Kadejo, et al. Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark. Am. J. Biomed. Life Sci. 2014, 2(1), 22-27. doi: 10.11648/j.ajbls.20140201.14

Copy | Download

AMA Style

James, Dorcas Bolanle, Sheneni, Victor Duniya, Kadejo, et al. Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark. Am J Biomed Life Sci. 2014;2(1):22-27. doi: 10.11648/j.ajbls.20140201.14

Copy | Download

@article{10.11648/j.ajbls.20140201.14,
  author = {James and Dorcas Bolanle and Sheneni and Victor Duniya and Kadejo and Olubukola Adetoro and Yatai and Kenneth Bobai},
  title = {Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {2},
  number = {1},
  pages = {22-27},
  doi = {10.11648/j.ajbls.20140201.14},
  url = {https://doi.org/10.11648/j.ajbls.20140201.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140201.14},
  abstract = {Phytochemical screening and antioxidant activities in different solvent extracts of Vitex doniana leaves, stem bark and root bark was carried out. The extracts were subjected  to various chemical test for phytochemical constituents, total phenolic contents were evaluated using Folin Ciocalteu method and their antioxidant activity was assayed through in- vitro radical scavenging activity using DPPH• assay, phytochemical screening of this study indicate the presence of cardiac glycosides, tannins, flavonoids, saponnins, alkaloids, steroids and terpenoids. The average total phenol content of leaves methanol and leaves ethanol extracts were significantly (P<0.05) higher when compared with the total polyphenol contents in the acetone and aqueous solvent extracts. In order of effectiveness (IC50) of the plant extracts the potent inhibitors was leaves ethanol extract (0.227mg/ml), follow by stem bark ethanol extract (0.236mg/ml), the least was the root ethanol extract (0.567mg/ml).This shows that Vitex doniana solvent extracts especially the ethanolic leaves extracts may be a potent source of natural antioxidant and it use in the management of diseases associated with oxidative stress is justify.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark
AU  - James
AU  - Dorcas Bolanle
AU  - Sheneni
AU  - Victor Duniya
AU  - Kadejo
AU  - Olubukola Adetoro
AU  - Yatai
AU  - Kenneth Bobai
Y1  - 2014/03/20
PY  - 2014
N1  - https://doi.org/10.11648/j.ajbls.20140201.14
DO  - 10.11648/j.ajbls.20140201.14
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 22
EP  - 27
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20140201.14
AB  - Phytochemical screening and antioxidant activities in different solvent extracts of Vitex doniana leaves, stem bark and root bark was carried out. The extracts were subjected  to various chemical test for phytochemical constituents, total phenolic contents were evaluated using Folin Ciocalteu method and their antioxidant activity was assayed through in- vitro radical scavenging activity using DPPH• assay, phytochemical screening of this study indicate the presence of cardiac glycosides, tannins, flavonoids, saponnins, alkaloids, steroids and terpenoids. The average total phenol content of leaves methanol and leaves ethanol extracts were significantly (P<0.05) higher when compared with the total polyphenol contents in the acetone and aqueous solvent extracts. In order of effectiveness (IC50) of the plant extracts the potent inhibitors was leaves ethanol extract (0.227mg/ml), follow by stem bark ethanol extract (0.236mg/ml), the least was the root ethanol extract (0.567mg/ml).This shows that Vitex doniana solvent extracts especially the ethanolic leaves extracts may be a potent source of natural antioxidant and it use in the management of diseases associated with oxidative stress is justify.
VL  - 2
IS  - 1
ER  -

Copy | Download