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Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark

Received: 20 January 2014     Published: 20 March 2014
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Abstract

Phytochemical screening and antioxidant activities in different solvent extracts of Vitex doniana leaves, stem bark and root bark was carried out. The extracts were subjected to various chemical test for phytochemical constituents, total phenolic contents were evaluated using Folin Ciocalteu method and their antioxidant activity was assayed through in- vitro radical scavenging activity using DPPH• assay, phytochemical screening of this study indicate the presence of cardiac glycosides, tannins, flavonoids, saponnins, alkaloids, steroids and terpenoids. The average total phenol content of leaves methanol and leaves ethanol extracts were significantly (P<0.05) higher when compared with the total polyphenol contents in the acetone and aqueous solvent extracts. In order of effectiveness (IC50) of the plant extracts the potent inhibitors was leaves ethanol extract (0.227mg/ml), follow by stem bark ethanol extract (0.236mg/ml), the least was the root ethanol extract (0.567mg/ml).This shows that Vitex doniana solvent extracts especially the ethanolic leaves extracts may be a potent source of natural antioxidant and it use in the management of diseases associated with oxidative stress is justify.

Published in American Journal of Biomedical and Life Sciences (Volume 2, Issue 1)
DOI 10.11648/j.ajbls.20140201.14
Page(s) 22-27
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Phytochemical, Antioxidant, Vitex Doniana, DPPH, Solvent Extraction

References
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    James, Dorcas Bolanle, Sheneni, Victor Duniya, Kadejo, et al. (2014). Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark. American Journal of Biomedical and Life Sciences, 2(1), 22-27. https://doi.org/10.11648/j.ajbls.20140201.14

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    ACS Style

    James; Dorcas Bolanle; Sheneni; Victor Duniya; Kadejo, et al. Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark. Am. J. Biomed. Life Sci. 2014, 2(1), 22-27. doi: 10.11648/j.ajbls.20140201.14

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    AMA Style

    James, Dorcas Bolanle, Sheneni, Victor Duniya, Kadejo, et al. Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark. Am J Biomed Life Sci. 2014;2(1):22-27. doi: 10.11648/j.ajbls.20140201.14

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  • @article{10.11648/j.ajbls.20140201.14,
      author = {James and Dorcas Bolanle and Sheneni and Victor Duniya and Kadejo and Olubukola Adetoro and Yatai and Kenneth Bobai},
      title = {Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {2},
      number = {1},
      pages = {22-27},
      doi = {10.11648/j.ajbls.20140201.14},
      url = {https://doi.org/10.11648/j.ajbls.20140201.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140201.14},
      abstract = {Phytochemical screening and antioxidant activities in different solvent extracts of Vitex doniana leaves, stem bark and root bark was carried out. The extracts were subjected  to various chemical test for phytochemical constituents, total phenolic contents were evaluated using Folin Ciocalteu method and their antioxidant activity was assayed through in- vitro radical scavenging activity using DPPH• assay, phytochemical screening of this study indicate the presence of cardiac glycosides, tannins, flavonoids, saponnins, alkaloids, steroids and terpenoids. The average total phenol content of leaves methanol and leaves ethanol extracts were significantly (P<0.05) higher when compared with the total polyphenol contents in the acetone and aqueous solvent extracts. In order of effectiveness (IC50) of the plant extracts the potent inhibitors was leaves ethanol extract (0.227mg/ml), follow by stem bark ethanol extract (0.236mg/ml), the least was the root ethanol extract (0.567mg/ml).This shows that Vitex doniana solvent extracts especially the ethanolic leaves extracts may be a potent source of natural antioxidant and it use in the management of diseases associated with oxidative stress is justify.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Phytochemical Screening, and in-Vitro Antioxidant Activities in Different Solvent Extracts of Vitex Doniana Leaves, Stem Bark and Root Bark
    AU  - James
    AU  - Dorcas Bolanle
    AU  - Sheneni
    AU  - Victor Duniya
    AU  - Kadejo
    AU  - Olubukola Adetoro
    AU  - Yatai
    AU  - Kenneth Bobai
    Y1  - 2014/03/20
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    DO  - 10.11648/j.ajbls.20140201.14
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 22
    EP  - 27
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20140201.14
    AB  - Phytochemical screening and antioxidant activities in different solvent extracts of Vitex doniana leaves, stem bark and root bark was carried out. The extracts were subjected  to various chemical test for phytochemical constituents, total phenolic contents were evaluated using Folin Ciocalteu method and their antioxidant activity was assayed through in- vitro radical scavenging activity using DPPH• assay, phytochemical screening of this study indicate the presence of cardiac glycosides, tannins, flavonoids, saponnins, alkaloids, steroids and terpenoids. The average total phenol content of leaves methanol and leaves ethanol extracts were significantly (P<0.05) higher when compared with the total polyphenol contents in the acetone and aqueous solvent extracts. In order of effectiveness (IC50) of the plant extracts the potent inhibitors was leaves ethanol extract (0.227mg/ml), follow by stem bark ethanol extract (0.236mg/ml), the least was the root ethanol extract (0.567mg/ml).This shows that Vitex doniana solvent extracts especially the ethanolic leaves extracts may be a potent source of natural antioxidant and it use in the management of diseases associated with oxidative stress is justify.
    VL  - 2
    IS  - 1
    ER  - 

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