Plasma Membrane-Derived Vesicles in Sickle Cell Disease: A Possible Indicator of the Continuous Endothelial Stimulation and/or Injury to Blood Cells

Samuel Antwi-Baffour; Abena Nyarkoah Wiredu; Ransford Kyeremeh; Seidu Abdulai Mahmood

doi:doi:10.11648/j.ajbls.20130104.14

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Plasma Membrane-Derived Vesicles in Sickle Cell Disease: A Possible Indicator of the Continuous Endothelial Stimulation and/or Injury to Blood Cells

Samuel Antwi-Baffour, Abena Nyarkoah Wiredu, Ransford Kyeremeh, Seidu Abdulai Mahmood

Published in American Journal of Biomedical and Life Sciences (Volume 1, Issue 4)

Received: 19 December 2013 Published: 30 December 2013

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Abstract

Plasma membrane-derived vesicles (PMVs) are released into circulation in response to normal and stress/pathogenic conditions. They are of tremendous significance for the prediction, diagnosis, and observation of the therapeutic success of many diseases. Knowledge of their functional properties would contribute to a better understanding of the pathological mechanisms leading to various diseases in which their levels are raised. The objective of this study was to quantify and compare the levels of PMVs in sickle cell disease patients (Hb SS and Hb SC) with non-sickle cell (Hb AA) subjects. The comparison will help us research and understand the processes that lead to their constitutive release in sickle cell disease patients than in their normal counterparts. A total of one hundred and fifty (150) sickle cell disease patients (study group) and blood donors (control group) that consented to partake in the study were recruited. There were 82 males and 68 females. Fifty (50) each of Hb SS, Hb SC and Hb AA samples were obtained. Sodium metabisulphite (sickling) test, Haemoglobin electrophoresis and quantification of PMVs were carried out on all the samples. The sickle cell disease patients had elevated levels; SS (38.89 ± 0.73 × 104/ml; p= 0.01) and SC (32.62 ± 1.18 × 104/ml; p = 0.01) as against the control subjects (Hb AA) who had average PMVs of 11.28 ± 0.29 × 104/ml PFP (mean ± SEM). It was concluded that both SS and SC (study) samples showed an increased count of PMVs as compared to the AA (control) samples, suggesting persistent endothelial stimulation and/or injury of blood cells leading to continuous shedding of PMVs in sickle cell disease patients.

Published in	American Journal of Biomedical and Life Sciences (Volume 1, Issue 4)
DOI	10.11648/j.ajbls.20130104.14
Page(s)	99-102
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

PMVs, Sickle Cell Disease, Electrophoresis, Flow Cytometer

References

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[2]	Kato GJ, Hebbel RP, Steinberg MH, Gladwin MT (2009). Vasculopathy in sickle cell disease: Biology, pathophysiology, genetics, translational medicine, and new research directions. Am J Haematol 84(9): 618-625.
[3]	Lionnet F, Hammoudi N, KS Stojanovic1, AvellinoV, Grateau G, Girot R and Haymann JP (2012). Hemoglobin SC disease complications: a clinical study of 179 cases. Haematol 97(8): 1136-1141.
[4]	Ballas SK, Lieff S, Benjamin LJ, Dampier CD, Heeney MM, Hoppe C, Johnson CS, Rogers ZR, Smith-Whitley K, Wang WC, Telen MJ (2010). Definitions of the phenotypic manifestations of sickle cell disease. Am J Haematol 85(1): 6-13.
[5]	Morel O, Hugel B, Jesel L, Zobairi F, Bareiss P, Freyssinet JM, Toti F (2004). Procoagulant membranous microparticles and atherothrombotic complications in diabetics. Arch Mal Coeur Vaiss 97(10): 1006-1012.
[6]	Boulanger CM, Scoazec A, Ebrahimian T, Henry P, Mathieu E, Tedgui A, Mallat Z (2001). Circulating microparticles from patients with myocardial infarction cause endothelial dysfunction. Circulation 104(22): 2649-52.
[7]	Baj-Krzyworzeka M, Majka M, Pratico D, Ratajczak J, Vilaire G, Kijowski J, Reca R, Janowska-Wieczorek A, Ratajczak MZ (2002). Platelet-derived microparticles stimulate proliferation, survival, adhesion, and chemotaxis of hematopoietic cells. Exp Hematol 30(5): 450-459.
[8]	Freyssinet JM, Toti F, Hugel B, Gidon-Jeangirard C, Kunzelmann C, Martínez MC, Meyer D (1999). Apoptosis in vascular disease. Thromb Haemost 82(2): 727-735.
[9]	Tan KT, Tayebjee MH, Lynd C, Blann AD, Lip GY (2005).Platelet microparticles and soluble P selectin in peripheral artery disease: relationship to extent of disease and platelet activation markers. Ann Med 37(1): 61-66.
[10]	Shet AS, Aras O, Gupta K, Hass MJ, Rausch DJ, Saba N, Koopmeiners L, Key NS, Hebbel RP (2003). Sickle blood contains tissue factor-positive microparticles derived from endothelial cells and monocytes. Blood 102 (7): 2678-2683.
[11]	Combes V, Coltel N, Alibert M, Van Eck M, Raymond C, Juhan-Vague I, Grau GE, Chimini G (2005). ABCA1 gene deletion protects against cerebral malaria: potential pathogenic role of microparticles in neuropathology. Am J Pathol 166(1): 295-302.
[12]	Allan D, Limbrick AR, Thomas P, Westerman MP (1981). Microvesicles from sickle erythrocytes and their relation to irreversible sickling. Br J Haematol 47: 383-390
[13]	Doeuvre L, Plawinski L, Toti F, Anglés-Cano E (2009). Cell-derived microparticles: a new challenge in neuroscience. Journal of Neurochemistry 110(2): 457-468.

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Samuel Antwi-Baffour, Abena Nyarkoah Wiredu, Ransford Kyeremeh, Seidu Abdulai Mahmood. (2013). Plasma Membrane-Derived Vesicles in Sickle Cell Disease: A Possible Indicator of the Continuous Endothelial Stimulation and/or Injury to Blood Cells. American Journal of Biomedical and Life Sciences, 1(4), 99-102. https://doi.org/10.11648/j.ajbls.20130104.14

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ACS Style

Samuel Antwi-Baffour; Abena Nyarkoah Wiredu; Ransford Kyeremeh; Seidu Abdulai Mahmood. Plasma Membrane-Derived Vesicles in Sickle Cell Disease: A Possible Indicator of the Continuous Endothelial Stimulation and/or Injury to Blood Cells. Am. J. Biomed. Life Sci. 2013, 1(4), 99-102. doi: 10.11648/j.ajbls.20130104.14

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AMA Style

Samuel Antwi-Baffour, Abena Nyarkoah Wiredu, Ransford Kyeremeh, Seidu Abdulai Mahmood. Plasma Membrane-Derived Vesicles in Sickle Cell Disease: A Possible Indicator of the Continuous Endothelial Stimulation and/or Injury to Blood Cells. Am J Biomed Life Sci. 2013;1(4):99-102. doi: 10.11648/j.ajbls.20130104.14

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@article{10.11648/j.ajbls.20130104.14,
  author = {Samuel Antwi-Baffour and Abena Nyarkoah Wiredu and Ransford Kyeremeh and Seidu Abdulai Mahmood},
  title = {Plasma Membrane-Derived Vesicles in Sickle Cell Disease: A Possible Indicator of the Continuous Endothelial Stimulation and/or Injury to Blood Cells},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {1},
  number = {4},
  pages = {99-102},
  doi = {10.11648/j.ajbls.20130104.14},
  url = {https://doi.org/10.11648/j.ajbls.20130104.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20130104.14},
  abstract = {Plasma membrane-derived vesicles (PMVs) are released into circulation in response to normal and stress/pathogenic conditions. They are of tremendous significance for the prediction, diagnosis, and observation of the therapeutic success of many diseases. Knowledge of their functional properties would contribute to a better understanding of the pathological mechanisms leading to various diseases in which their levels are raised. The objective of this study was to quantify and compare the levels of PMVs in sickle cell disease patients (Hb SS and Hb SC) with non-sickle cell (Hb AA) subjects.  The comparison will help us research and understand the processes that lead to their constitutive release in sickle cell disease patients than in their normal counterparts. A total of one hundred and fifty (150) sickle cell disease patients (study group) and blood donors (control group) that consented to partake in the study were recruited. There were 82 males and 68 females. Fifty (50) each of Hb SS, Hb SC and Hb AA samples were obtained. Sodium metabisulphite (sickling) test, Haemoglobin electrophoresis and quantification of PMVs were carried out on all the samples. The sickle cell disease patients had elevated levels; SS (38.89 ± 0.73 × 104/ml; p= 0.01) and SC (32.62 ± 1.18 × 104/ml; p = 0.01) as against the control subjects (Hb AA) who had average PMVs of 11.28 ± 0.29 × 104/ml PFP (mean ± SEM). It was concluded that both SS and SC (study) samples showed an increased count of PMVs as compared to the AA (control) samples, suggesting persistent endothelial stimulation and/or injury of blood cells leading to continuous shedding of PMVs in sickle cell disease patients.},
 year = {2013}
}

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TY  - JOUR
T1  - Plasma Membrane-Derived Vesicles in Sickle Cell Disease: A Possible Indicator of the Continuous Endothelial Stimulation and/or Injury to Blood Cells
AU  - Samuel Antwi-Baffour
AU  - Abena Nyarkoah Wiredu
AU  - Ransford Kyeremeh
AU  - Seidu Abdulai Mahmood
Y1  - 2013/12/30
PY  - 2013
N1  - https://doi.org/10.11648/j.ajbls.20130104.14
DO  - 10.11648/j.ajbls.20130104.14
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 99
EP  - 102
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20130104.14
AB  - Plasma membrane-derived vesicles (PMVs) are released into circulation in response to normal and stress/pathogenic conditions. They are of tremendous significance for the prediction, diagnosis, and observation of the therapeutic success of many diseases. Knowledge of their functional properties would contribute to a better understanding of the pathological mechanisms leading to various diseases in which their levels are raised. The objective of this study was to quantify and compare the levels of PMVs in sickle cell disease patients (Hb SS and Hb SC) with non-sickle cell (Hb AA) subjects.  The comparison will help us research and understand the processes that lead to their constitutive release in sickle cell disease patients than in their normal counterparts. A total of one hundred and fifty (150) sickle cell disease patients (study group) and blood donors (control group) that consented to partake in the study were recruited. There were 82 males and 68 females. Fifty (50) each of Hb SS, Hb SC and Hb AA samples were obtained. Sodium metabisulphite (sickling) test, Haemoglobin electrophoresis and quantification of PMVs were carried out on all the samples. The sickle cell disease patients had elevated levels; SS (38.89 ± 0.73 × 104/ml; p= 0.01) and SC (32.62 ± 1.18 × 104/ml; p = 0.01) as against the control subjects (Hb AA) who had average PMVs of 11.28 ± 0.29 × 104/ml PFP (mean ± SEM). It was concluded that both SS and SC (study) samples showed an increased count of PMVs as compared to the AA (control) samples, suggesting persistent endothelial stimulation and/or injury of blood cells leading to continuous shedding of PMVs in sickle cell disease patients.
VL  - 1
IS  - 4
ER  -

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Author Information

Samuel Antwi-Baffour

Department of Medical Laboratory Sciences, School of Allied Health Sciences, College of Health Sciences, University of Ghana, P. O. Box KB 143, Korle-Bu, Accra, Ghana
Abena Nyarkoah Wiredu

The Central Laboratory, Korle-bu Teaching Hospital, Accra, Ghana
Ransford Kyeremeh

Department of Medical Laboratory Sciences, School of Allied Health Sciences, College of Health Sciences, University of Ghana, P. O. Box KB 143, Korle-Bu, Accra, Ghana
Seidu Abdulai Mahmood

Department of Medical Laboratory Sciences, School of Allied Health Sciences, College of Health Sciences, University of Ghana, P. O. Box KB 143, Korle-Bu, Accra, Ghana

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APA Style

Samuel Antwi-Baffour, Abena Nyarkoah Wiredu, Ransford Kyeremeh, Seidu Abdulai Mahmood. (2013). Plasma Membrane-Derived Vesicles in Sickle Cell Disease: A Possible Indicator of the Continuous Endothelial Stimulation and/or Injury to Blood Cells. American Journal of Biomedical and Life Sciences, 1(4), 99-102. https://doi.org/10.11648/j.ajbls.20130104.14

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ACS Style

Samuel Antwi-Baffour; Abena Nyarkoah Wiredu; Ransford Kyeremeh; Seidu Abdulai Mahmood. Plasma Membrane-Derived Vesicles in Sickle Cell Disease: A Possible Indicator of the Continuous Endothelial Stimulation and/or Injury to Blood Cells. Am. J. Biomed. Life Sci. 2013, 1(4), 99-102. doi: 10.11648/j.ajbls.20130104.14

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AMA Style

Samuel Antwi-Baffour, Abena Nyarkoah Wiredu, Ransford Kyeremeh, Seidu Abdulai Mahmood. Plasma Membrane-Derived Vesicles in Sickle Cell Disease: A Possible Indicator of the Continuous Endothelial Stimulation and/or Injury to Blood Cells. Am J Biomed Life Sci. 2013;1(4):99-102. doi: 10.11648/j.ajbls.20130104.14

Copy | Download

@article{10.11648/j.ajbls.20130104.14,
  author = {Samuel Antwi-Baffour and Abena Nyarkoah Wiredu and Ransford Kyeremeh and Seidu Abdulai Mahmood},
  title = {Plasma Membrane-Derived Vesicles in Sickle Cell Disease: A Possible Indicator of the Continuous Endothelial Stimulation and/or Injury to Blood Cells},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {1},
  number = {4},
  pages = {99-102},
  doi = {10.11648/j.ajbls.20130104.14},
  url = {https://doi.org/10.11648/j.ajbls.20130104.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20130104.14},
  abstract = {Plasma membrane-derived vesicles (PMVs) are released into circulation in response to normal and stress/pathogenic conditions. They are of tremendous significance for the prediction, diagnosis, and observation of the therapeutic success of many diseases. Knowledge of their functional properties would contribute to a better understanding of the pathological mechanisms leading to various diseases in which their levels are raised. The objective of this study was to quantify and compare the levels of PMVs in sickle cell disease patients (Hb SS and Hb SC) with non-sickle cell (Hb AA) subjects.  The comparison will help us research and understand the processes that lead to their constitutive release in sickle cell disease patients than in their normal counterparts. A total of one hundred and fifty (150) sickle cell disease patients (study group) and blood donors (control group) that consented to partake in the study were recruited. There were 82 males and 68 females. Fifty (50) each of Hb SS, Hb SC and Hb AA samples were obtained. Sodium metabisulphite (sickling) test, Haemoglobin electrophoresis and quantification of PMVs were carried out on all the samples. The sickle cell disease patients had elevated levels; SS (38.89 ± 0.73 × 104/ml; p= 0.01) and SC (32.62 ± 1.18 × 104/ml; p = 0.01) as against the control subjects (Hb AA) who had average PMVs of 11.28 ± 0.29 × 104/ml PFP (mean ± SEM). It was concluded that both SS and SC (study) samples showed an increased count of PMVs as compared to the AA (control) samples, suggesting persistent endothelial stimulation and/or injury of blood cells leading to continuous shedding of PMVs in sickle cell disease patients.},
 year = {2013}
}

Copy | Download

TY  - JOUR
T1  - Plasma Membrane-Derived Vesicles in Sickle Cell Disease: A Possible Indicator of the Continuous Endothelial Stimulation and/or Injury to Blood Cells
AU  - Samuel Antwi-Baffour
AU  - Abena Nyarkoah Wiredu
AU  - Ransford Kyeremeh
AU  - Seidu Abdulai Mahmood
Y1  - 2013/12/30
PY  - 2013
N1  - https://doi.org/10.11648/j.ajbls.20130104.14
DO  - 10.11648/j.ajbls.20130104.14
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 99
EP  - 102
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20130104.14
AB  - Plasma membrane-derived vesicles (PMVs) are released into circulation in response to normal and stress/pathogenic conditions. They are of tremendous significance for the prediction, diagnosis, and observation of the therapeutic success of many diseases. Knowledge of their functional properties would contribute to a better understanding of the pathological mechanisms leading to various diseases in which their levels are raised. The objective of this study was to quantify and compare the levels of PMVs in sickle cell disease patients (Hb SS and Hb SC) with non-sickle cell (Hb AA) subjects.  The comparison will help us research and understand the processes that lead to their constitutive release in sickle cell disease patients than in their normal counterparts. A total of one hundred and fifty (150) sickle cell disease patients (study group) and blood donors (control group) that consented to partake in the study were recruited. There were 82 males and 68 females. Fifty (50) each of Hb SS, Hb SC and Hb AA samples were obtained. Sodium metabisulphite (sickling) test, Haemoglobin electrophoresis and quantification of PMVs were carried out on all the samples. The sickle cell disease patients had elevated levels; SS (38.89 ± 0.73 × 104/ml; p= 0.01) and SC (32.62 ± 1.18 × 104/ml; p = 0.01) as against the control subjects (Hb AA) who had average PMVs of 11.28 ± 0.29 × 104/ml PFP (mean ± SEM). It was concluded that both SS and SC (study) samples showed an increased count of PMVs as compared to the AA (control) samples, suggesting persistent endothelial stimulation and/or injury of blood cells leading to continuous shedding of PMVs in sickle cell disease patients.
VL  - 1
IS  - 4
ER  -

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