Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children

Kohinoor Akter Raton; Md. Asif Hossain; Rashed Noor

doi:doi:10.11648/j.ajbls.20130103.13

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Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children

Kohinoor Akter Raton, Md. Asif Hossain, Rashed Noor

Published in American Journal of Biomedical and Life Sciences (Volume 1, Issue 3)

Received: 21 October 2013 Published: 20 November 2013

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Abstract

Molecular diagnostic methods offer a rapid and more sensitive strategy with high selectivity compared to the traditional immunoassays and culture techniques. Present study attempted to employ the molecular approach for the diagnosis of respiratory viral- and bacterial infections among the pneumonia infected children in Dhaka Metropolis. Samples were aseptically collected, processed and analyzed by using Multiplex Real Time PCR technique to detect respiratory pathogens within a time frame of February 2013-June 2013. Influenza viruses, human meta-pneumo viruses, respiratory syncytial viruses and other potential infective viruses were diagnosed. Among the infective bacterial population, presence of Mycoplasma pneumoniae, Chlamydia pneumoniae, Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Legionella species, Salmonella species were detected. Among the nasopharyngeal and orpharyngeal (NPOP) samples, Influenza B and Streptococcus pneumoniae were found to be dominating (34.8% and 30.4%, respectively) whereas the induced sputum (IS) samples were 100% positive for S. pneumoniae followed by around 50% prevalence with equine arteritis virus, para influenza virus and the respiratory syncytial viruses A and B. Considering the spatial identification of an array of microorganisms, results of the current study sufficiently may endorse such molecular method of diagnosis for the betterment of public health.

Published in	American Journal of Biomedical and Life Sciences (Volume 1, Issue 3)
DOI	10.11648/j.ajbls.20130103.13
Page(s)	53-57
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Respiratory Pathogens, Pneumonia, Molecular Diagnostic Methods, Public Health

References

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APA Style

Kohinoor Akter Raton, Md. Asif Hossain, Rashed Noor. (2013). Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children. American Journal of Biomedical and Life Sciences, 1(3), 53-57. https://doi.org/10.11648/j.ajbls.20130103.13

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ACS Style

Kohinoor Akter Raton; Md. Asif Hossain; Rashed Noor. Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children. Am. J. Biomed. Life Sci. 2013, 1(3), 53-57. doi: 10.11648/j.ajbls.20130103.13

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AMA Style

Kohinoor Akter Raton, Md. Asif Hossain, Rashed Noor. Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children. Am J Biomed Life Sci. 2013;1(3):53-57. doi: 10.11648/j.ajbls.20130103.13

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@article{10.11648/j.ajbls.20130103.13,
  author = {Kohinoor Akter Raton and Md. Asif Hossain and Rashed Noor},
  title = {Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {1},
  number = {3},
  pages = {53-57},
  doi = {10.11648/j.ajbls.20130103.13},
  url = {https://doi.org/10.11648/j.ajbls.20130103.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20130103.13},
  abstract = {Molecular diagnostic methods offer a rapid and more sensitive strategy with high selectivity compared to the traditional immunoassays and culture techniques. Present study attempted to employ the molecular approach for the diagnosis of respiratory viral- and bacterial infections among the pneumonia infected children in Dhaka Metropolis. Samples were aseptically collected, processed and analyzed by using Multiplex Real Time PCR technique to detect respiratory pathogens within a time frame of February 2013-June 2013. Influenza viruses, human meta-pneumo viruses, respiratory syncytial viruses and other potential infective viruses were diagnosed. Among the infective bacterial population, presence of Mycoplasma pneumoniae, Chlamydia pneumoniae, Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Legionella species, Salmonella species were detected. Among the nasopharyngeal and orpharyngeal (NPOP) samples, Influenza B and Streptococcus pneumoniae were found to be dominating (34.8% and 30.4%, respectively) whereas the induced sputum (IS) samples were 100% positive for S. pneumoniae followed by around 50% prevalence with equine arteritis virus, para influenza virus and the respiratory syncytial viruses A and B. Considering the spatial identification of an array of microorganisms, results of the current study sufficiently may endorse such molecular method of diagnosis for the betterment of public health.},
 year = {2013}
}

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TY  - JOUR
T1  - Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children
AU  - Kohinoor Akter Raton
AU  - Md. Asif Hossain
AU  - Rashed Noor
Y1  - 2013/11/20
PY  - 2013
N1  - https://doi.org/10.11648/j.ajbls.20130103.13
DO  - 10.11648/j.ajbls.20130103.13
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 53
EP  - 57
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20130103.13
AB  - Molecular diagnostic methods offer a rapid and more sensitive strategy with high selectivity compared to the traditional immunoassays and culture techniques. Present study attempted to employ the molecular approach for the diagnosis of respiratory viral- and bacterial infections among the pneumonia infected children in Dhaka Metropolis. Samples were aseptically collected, processed and analyzed by using Multiplex Real Time PCR technique to detect respiratory pathogens within a time frame of February 2013-June 2013. Influenza viruses, human meta-pneumo viruses, respiratory syncytial viruses and other potential infective viruses were diagnosed. Among the infective bacterial population, presence of Mycoplasma pneumoniae, Chlamydia pneumoniae, Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Legionella species, Salmonella species were detected. Among the nasopharyngeal and orpharyngeal (NPOP) samples, Influenza B and Streptococcus pneumoniae were found to be dominating (34.8% and 30.4%, respectively) whereas the induced sputum (IS) samples were 100% positive for S. pneumoniae followed by around 50% prevalence with equine arteritis virus, para influenza virus and the respiratory syncytial viruses A and B. Considering the spatial identification of an array of microorganisms, results of the current study sufficiently may endorse such molecular method of diagnosis for the betterment of public health.
VL  - 1
IS  - 3
ER  -

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Author Information

Kohinoor Akter Raton

Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Md. Asif Hossain

Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Rashed Noor

Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

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APA Style

Kohinoor Akter Raton, Md. Asif Hossain, Rashed Noor. (2013). Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children. American Journal of Biomedical and Life Sciences, 1(3), 53-57. https://doi.org/10.11648/j.ajbls.20130103.13

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ACS Style

Kohinoor Akter Raton; Md. Asif Hossain; Rashed Noor. Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children. Am. J. Biomed. Life Sci. 2013, 1(3), 53-57. doi: 10.11648/j.ajbls.20130103.13

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AMA Style

Kohinoor Akter Raton, Md. Asif Hossain, Rashed Noor. Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children. Am J Biomed Life Sci. 2013;1(3):53-57. doi: 10.11648/j.ajbls.20130103.13

Copy | Download

@article{10.11648/j.ajbls.20130103.13,
  author = {Kohinoor Akter Raton and Md. Asif Hossain and Rashed Noor},
  title = {Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {1},
  number = {3},
  pages = {53-57},
  doi = {10.11648/j.ajbls.20130103.13},
  url = {https://doi.org/10.11648/j.ajbls.20130103.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20130103.13},
  abstract = {Molecular diagnostic methods offer a rapid and more sensitive strategy with high selectivity compared to the traditional immunoassays and culture techniques. Present study attempted to employ the molecular approach for the diagnosis of respiratory viral- and bacterial infections among the pneumonia infected children in Dhaka Metropolis. Samples were aseptically collected, processed and analyzed by using Multiplex Real Time PCR technique to detect respiratory pathogens within a time frame of February 2013-June 2013. Influenza viruses, human meta-pneumo viruses, respiratory syncytial viruses and other potential infective viruses were diagnosed. Among the infective bacterial population, presence of Mycoplasma pneumoniae, Chlamydia pneumoniae, Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Legionella species, Salmonella species were detected. Among the nasopharyngeal and orpharyngeal (NPOP) samples, Influenza B and Streptococcus pneumoniae were found to be dominating (34.8% and 30.4%, respectively) whereas the induced sputum (IS) samples were 100% positive for S. pneumoniae followed by around 50% prevalence with equine arteritis virus, para influenza virus and the respiratory syncytial viruses A and B. Considering the spatial identification of an array of microorganisms, results of the current study sufficiently may endorse such molecular method of diagnosis for the betterment of public health.},
 year = {2013}
}

Copy | Download

TY  - JOUR
T1  - Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children
AU  - Kohinoor Akter Raton
AU  - Md. Asif Hossain
AU  - Rashed Noor
Y1  - 2013/11/20
PY  - 2013
N1  - https://doi.org/10.11648/j.ajbls.20130103.13
DO  - 10.11648/j.ajbls.20130103.13
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 53
EP  - 57
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20130103.13
AB  - Molecular diagnostic methods offer a rapid and more sensitive strategy with high selectivity compared to the traditional immunoassays and culture techniques. Present study attempted to employ the molecular approach for the diagnosis of respiratory viral- and bacterial infections among the pneumonia infected children in Dhaka Metropolis. Samples were aseptically collected, processed and analyzed by using Multiplex Real Time PCR technique to detect respiratory pathogens within a time frame of February 2013-June 2013. Influenza viruses, human meta-pneumo viruses, respiratory syncytial viruses and other potential infective viruses were diagnosed. Among the infective bacterial population, presence of Mycoplasma pneumoniae, Chlamydia pneumoniae, Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Legionella species, Salmonella species were detected. Among the nasopharyngeal and orpharyngeal (NPOP) samples, Influenza B and Streptococcus pneumoniae were found to be dominating (34.8% and 30.4%, respectively) whereas the induced sputum (IS) samples were 100% positive for S. pneumoniae followed by around 50% prevalence with equine arteritis virus, para influenza virus and the respiratory syncytial viruses A and B. Considering the spatial identification of an array of microorganisms, results of the current study sufficiently may endorse such molecular method of diagnosis for the betterment of public health.
VL  - 1
IS  - 3
ER  -

Copy | Download