Abstract: Objective: To evaluate the antimicrobial properties of hydroethanol extracts of Bauhinia rufescens L. and Euphorbia hirta L. Methodology and results: The hydroethanol extracts of Bauhinia rufescens L. and Euphorbia hirta L.; two plants of the Chadian traditional pharmacopoeia used in the treatment of infantile diarrhoea and gastroenteritis in adults were submitted to in vitro tests in order to highlight their antibacterial and antifungal properties. The method of microdilution in liquid medium coupled with spreading on agar medium was used for the tests. The microbial strains used consisted of 13 hospital bacterial strains and 6 reference strains including a yeast strain of the Candida albicans ATCC 90028 species. The results obtained confirm the antimicrobial properties of Bauhinia rufescens L. and Euphorbia hirta L. because at 50 mg/ml, they inhibit 100% the growth of gram positive and Gram-negative bacilli tested. On the other hand, for Candida albicans ATCC 90028, it is only the extract of Euphorbia hirta that was active at 50 mg/ml. Conclusion: In view of these results, we can say that these plants have an antibacterial activity and that their use in traditional phytotherapy is justified.Abstract: Objective: To evaluate the antimicrobial properties of hydroethanol extracts of Bauhinia rufescens L. and Euphorbia hirta L. Methodology and results: The hydroethanol extracts of Bauhinia rufescens L. and Euphorbia hirta L.; two plants of the Chadian traditional pharmacopoeia used in the treatment of infantile diarrhoea and gastroenteritis in adult...Show More
Abstract: The objective of the study was to document traditional knowledge on medicinal plants in north Achefer district. This study was conducted using semi-structured interviews using purposeful and random sampling methods. The collected data was analyzed using IBM SPSS statics 21software. A total of 65 informants 59 male and 6 female were interviewed from 5 kebele of north Achefer district. Among 65 informants only 12 (18.5%) individuals are traditional healers and the rest 53 (81.5%) persons were not traditional healers. A total of 74 medicinal plants were recorded for treating human and animal ailments through oral, dermal and nasal routs. From the collected medicinal plant species, 98% / of them were identified and the rest 2% / medicinal plant species were listed by their local name. Among 39 plant families the most frequently used plant species reported were Solanaceae 5 (7.4%) and Euphorbiaceae 5 (7.4%), Fabaceae 4 (5.9%) and the least Lamiaceae 3 (4.4%). In the study area medicinal plants affect by different factors, the main factors were human activity (69.2%) by cutting; agricultural explanation, cultivating introduced species and natural factors (20%) drought, erosion, hail and the rest (10.8%) of respondents were reported that factors affected medicinal plants in the area. North Achefer woreda is rich in indigenous knowledge associated with medicinal plant but the distribution and diversity of medicinal plants have been declined at alarming rate. So attention should given and use best conservation methods to protect species from loss.Abstract: The objective of the study was to document traditional knowledge on medicinal plants in north Achefer district. This study was conducted using semi-structured interviews using purposeful and random sampling methods. The collected data was analyzed using IBM SPSS statics 21software. A total of 65 informants 59 male and 6 female were interviewed from...Show More
Abstract: Background: In previous studies, a special extract from Cistus × incanus L. Pandalis (Cystus Pandalis® extract) has already proven to be effective against SARS-CoV-2 in vitro. We assume that an effect was also likely against the new SARS-CoV-2 variants, like the “British” alpha variant (B.1.1.7) as well as against the “South African” beta variant (B.1.351). Methods: To verify this, we investigated the inhibition of the cytopathic effect (CPE) of the coronaviruses by the Cystus Pandalis® extract in a cell model with human intestinal cells (Caco-2). We inoculated viral pre-treated cells with the herbal extract (A), and we mixed pre-treated viruses with the herbal extract in the cell cultures (B). Result: We observed an almost complete inhibition of virus growth by Cystus Pandalis® extract at concentrations of more than 100 μg/ml. The calculated IC50 (mean inhibitory concentration) for the “British” alpha variant (B.1.1.7) as well as for the “South African” beta variant (B.1.351) is below 50 μg/ml. There was no significant difference in the results in the two different ways of treatment. Conclusion: Extract from Cistus × incanus L. Pandalis (Cystus Pandalis® extract) can prevent in in-vitro cell cultures infections with SARS-CoV-2. Due to the high in vitro activity against the new variants, it also appears reasonable in the future to use Cystus Pandalis® as prophylaxis against infections with SARS-CoV-2. A development of resistance is unlikely. People tolerate the extract very well. It has no significant side effects.Abstract: Background: In previous studies, a special extract from Cistus × incanus L. Pandalis (Cystus Pandalis® extract) has already proven to be effective against SARS-CoV-2 in vitro. We assume that an effect was also likely against the new SARS-CoV-2 variants, like the “British” alpha variant (B.1.1.7) as well as against the “South African” beta variant (...Show More
