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The Prognostic Value of Apoptotic Marker (CD95) in Adult Acute Leukemias
Eman Abd Al-Halim,
M. Raouf Emam,
Abdulwahab Ali Abuderman
Issue:
Volume 1, Issue 1, December 2016
Pages:
1-5
Received:
4 November 2016
Accepted:
3 December 2016
Published:
7 January 2017
DOI:
10.11648/j.ijcocr.20160101.11
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Abstract: Fas-mediated cell death is involved in drug-induced apoptosis in various cell types. Hence, failure of apoptosis could lead to chemoresistance in acute leukemia. The participants of this study were 80 adult acute leukemia patients classified as follows: 40 acute myeloid leukemia (AML) patients, 40 acute lymphoblastic leukemia (ALL) patients. In addition, 10 healthy controls were also included in the study. Fas expression was measured using flow cytometry. The mean value of Fas expression by blast cells from AML patients at diagnosis was 41.72 ± 10.3%. AML patients were divided into the Fas-positive group [30 patients (72.5%)] and the Fas-negative group [10 patients (27.5%)]. The mean value of expression increased significantly in M5 (52.91 ± 11.3%) with highly significant differences (P < 0.001) between Fas expression levels in different FAB subtypes of AML. The mean value of Fas expression in ALL patients was 43.87± 11.5%. 23 (57.5%) patients were positive for Fas expression, whereas 17 (42.5%) were negative. Fas expression was positive in 14/24 (63.2%) precursor B-ALL patients and in 12/16 (84.6%) T-ALL patients. The mean value of Fas expression was significantly higher (P = 0.039) in T-ALL (55.15 ± 7.8%) in comparison with precursor B-ALL (34.47 ± 5.76%). The mean value of Fas expression by blast cells from AML and ALL, patients at diagnosis was 41.72± 10.3 and 43.87 ± 11.5. We can conclude that Fas receptor expression on blast cells from ALL and AML patients could serve as an independent prognostic factor.
Abstract: Fas-mediated cell death is involved in drug-induced apoptosis in various cell types. Hence, failure of apoptosis could lead to chemoresistance in acute leukemia. The participants of this study were 80 adult acute leukemia patients classified as follows: 40 acute myeloid leukemia (AML) patients, 40 acute lymphoblastic leukemia (ALL) patients. In add...
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Comparative Modeling and Molecular Docking Study of P53 and AKT1, Genes of Lung Cancer Pathways
Asif Mir,
Syeda Naqsh e Zahra,
Sobiah Rauf
Issue:
Volume 1, Issue 1, December 2016
Pages:
6-14
Received:
15 October 2016
Accepted:
3 December 2016
Published:
9 January 2017
DOI:
10.11648/j.ijcocr.20160101.12
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Abstract: The fundamentals of structure-based drug designing rely on protein-ligand interactions, which play a significant role to open a gateway from identification of active residues and development of potential drugs. The endeavor behind this work is to select most susceptible genes p53 and AKT1 which plays a vital role in lung cancer pathogencity. In a queue to deliberate the crucial role of these genes in-silico experimental strategy was adopted. 3-D structure of p53 generated by YASARA showed 50.9% sequence identity with 2PCX-A and Z-score of -0.276 while AKT1 showed 66.3% sequence identity with 3QKL-A and Z-score of 0.036. Mutational analysis revealed that R273L and C275Y mutations of p53 destabilize the DNA binding domain, while E17K mutation of AKT1directly affect the binding of the ligand as this residues lines the pocket. Molecular docking was performed using ligands Staurosporine and Nutlin-3 retrieved form ZINC database. Blind docking experiment revealed that p53 involve non polar (Leu206, Leu188, Pro190), acidic (Glu204, Tyr 205) and basic (Arg202) as most interacting residues. AKT1 interactions with ligand Staurosporine revealed nonpolar (Val164, Phe438, Phe442, Phe 236, Phe 237, Phe 161), polar (Gly159, Gly157, Gly234, Gly 278), basic (Lys163, Lys158, Lys 276, Lys 179), acidic (Asp439, Glu278) as most interacting residues. It is assumed that current study will play a significant contribution to design potential drug inhibitors by utilizing most interactive residue information with Nutlin-3 and Staurosporine ligands to restrain the interaction between p53 pathways and epidermal growth pathways. Structural based receptor-ligand interactions likely to be used against anti-cancer therapy.
Abstract: The fundamentals of structure-based drug designing rely on protein-ligand interactions, which play a significant role to open a gateway from identification of active residues and development of potential drugs. The endeavor behind this work is to select most susceptible genes p53 and AKT1 which plays a vital role in lung cancer pathogencity. In a q...
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Clinical and Pathological Features of Small Intestine Tumors
Zheng Wang,
Shijie Huang,
Jianhua Lin,
Yixiong Lin,
Jie Zhou
Issue:
Volume 1, Issue 1, December 2016
Pages:
15-25
Received:
12 November 2016
Accepted:
17 December 2016
Published:
10 January 2017
DOI:
10.11648/j.ijcocr.20160101.13
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Abstract: Small intestinal tumor is a rare disease, with atypical symptoms, difficult for early diagnosis, with Controversial treatment. This study was to provide reference for prevention, diagnosis and treatment of this tumor. 57 cases of small intestinal tumor were reviewed. Clinicopathological features, survival and prognosis were followed up. Rates were compared using chi-square test, means between multiple groups were compared using ANOVA, OS was analyzed by Kaplan–Meier, COX proportional hazard model was for analyzing the prognosis. Number of cases showed increasing trend since 2000. Female exceeded Male except for jejunum tumor, but without significant difference; There were no significant difference between cases of more and less than 60 years of age. Exept for rare pathological types of carcinoid, female exceeded male, but without significant difference. Abdominal pain was the most common main clinical manifestation. 26.3% patients were found during treatment for intestinal obstruction. Jaundice was the manifestation of duodenal tumors, especially located ampulla of Vater. Anal stopping exhaust defecation was main clinical manifestation of ileal tumors. Main clinical symptom of adenocarcinoma was jaundice. Stromal tumor had clinical manifestations of diarrhea. Abdominal pain was primary clinical manifestation of other extremely rare pathological types. Gastrointestinal endoscopy was the most common examinational procedure. In Conclusions, Adenocarcinoma was the most common type among small intestinal tumors. Age had no effect on the choice of operative or chemotherapy option. Gender or age was independent prognostic factors for OS. Surgery was the most effective treatment, role of chemotherapy displayed no survival benefit.
Abstract: Small intestinal tumor is a rare disease, with atypical symptoms, difficult for early diagnosis, with Controversial treatment. This study was to provide reference for prevention, diagnosis and treatment of this tumor. 57 cases of small intestinal tumor were reviewed. Clinicopathological features, survival and prognosis were followed up. Rates were ...
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Clinical Study on the Application of Yashiro and RH Catheter for Transcatheter Arterial Chemoembolization
Issue:
Volume 1, Issue 1, December 2016
Pages:
26-29
Received:
5 December 2016
Accepted:
21 December 2016
Published:
10 January 2017
DOI:
10.11648/j.ijcocr.20160101.14
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Abstract: We analyze the impact on the post-operative syndrome of application of Yashiro and RH catheters to TACE. We analyze the superselective success rate of Yashiro and RH catheters in terms of hours and epiphenomenon and the impact on the post-operative syndrome of application of both of these catheters using blood test, objective indices. The superselection was successful 45 times using Yashiro catheter and the success rate was 93.75%, and 41 times using RH catheter, and the success rate 78.84%. The average time until the superselection was 347.39±29.44(s) in Yashiro catheter and 652.97±203.36(s) in RH catheter. And there were 6 cases (16.6%) of the prolongation of the operating time due to vascular spasm in RH catheter group. In Yashiro catheter group the moderate increase of transaminase and ESR was observed 7 days after operation, then it returned to the baseline on 14th day. But in RH catheter group the severe increase of these indices was observed 7 days after operation, and then it has been decreasing gradually on 14th day.(p<0.05). Our study suggests that TACE using Yashiro catheter is relatively safe approach in terms of postoperative syndrome, because it increases success rate of superselection and reduces the overall operating time.
Abstract: We analyze the impact on the post-operative syndrome of application of Yashiro and RH catheters to TACE. We analyze the superselective success rate of Yashiro and RH catheters in terms of hours and epiphenomenon and the impact on the post-operative syndrome of application of both of these catheters using blood test, objective indices. The supersele...
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Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer
Maksym Prylutskyi,
Nickolaj Starodub,
Nadiia Bilko
Issue:
Volume 1, Issue 1, December 2016
Pages:
30-35
Received:
13 November 2016
Accepted:
3 December 2016
Published:
17 January 2017
DOI:
10.11648/j.ijcocr.20160101.15
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Abstract: The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. It was performed two methods of analysis: direct, when immobilized specific antibodies interacted with appropriate antigens and competitive, when the one part of antigens was conjugated with protein and competed for binding with specific antibodies with free antigens. In relation to direct analysis it was shown the dependence of sensitivity of biosensor response from the concentration of polyamines in range of 10-100 ng /ml. Concerning the competitive method of analysis, the linear region of sensitivity was in range of 0,3-80 ng/ml and 0,01-90 ng/ml for spermine and spermidine respectively.
Abstract: The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time ...
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Environmentally-Mediated Epigenetic Effects: Uncovering the Fertile Soil in the Development of Pediatric Cancer
Ahmed Mohammed Morsy,
Eman Ahmed Hasan,
Ameer Mohammed Abuelgheet,
Ahmed Salaheldeen Hassan
Issue:
Volume 1, Issue 1, December 2016
Pages:
36-41
Received:
20 December 2016
Accepted:
30 December 2016
Published:
17 January 2017
DOI:
10.11648/j.ijcocr.20160101.16
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Abstract: Environmentally-induced epigenetic changes of gene regulation could result from chronic, lifelong exposure, to low doses of environmental toxicants, such as chemicals including, tobacco smoking and endocrine disrupting compounds, or to other environmental factors such as nutritional changes, and lifestyle-related conditions. These environmentally-acquired epigenetic marks may influence the control of gene regulation through DNA methylation, histone modification, or through a large set of non-coding RNAs (ncRNAs). These epigenetic effects might be passed on to the developing embryo and child as inheritable non-genetic marks, which recapitulate previous lifelong history of exposure to environmental influences that start from the stage of primordial germ cell, passing through the maturing germ cell, and ending by the zygote stage. This involves the paternally transmitted information on the sperm that contribute to modulating embryogenesis functions and later childhood development, in concert with, the maternally transmitted information encountered by the exposure to a large milieu of environmental factors either periconceptionally or during lactation period.
Abstract: Environmentally-induced epigenetic changes of gene regulation could result from chronic, lifelong exposure, to low doses of environmental toxicants, such as chemicals including, tobacco smoking and endocrine disrupting compounds, or to other environmental factors such as nutritional changes, and lifestyle-related conditions. These environmentally-a...
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