Multidrug-resistant pathogens are now emergent worldwide and pose a serious threat to disease prevention and treatment. The World Health Organization declared anti-microbial resistance as a major peril to human beings. Staphylococcus is part of the normal microbiome of humans and are responsible for opportunistic infections. Like S. aureus, Coagulase-negative staphylococcus are also clinically important as the causal agents of severe diseases, nosocomial infections, catheter-associated infection, bacteremia, septicemia. Methicillin resistant Staphylococci species are widely known and methicillin resistant CoNS, known as MR-CoNS, have been found to carry the mecA gene. Various virulence factors help these organisms in their pathogenicity and antibiotic-resistance is aided by the presence of efflux pumps. In our study, we have isolated 45 isolates from sewage water which were tentatively identified as CoNS based on biochemical characteristics. Then virulence and antibiotics susceptibility patterns were screened by standard protocols. Multiple drug resistant CoNS were found in our study and in silico analysis of an annotated protein sequence of the efflux pump SepA of Pseudomonas putida was carried out. Sequence analysis of the efflux protein gene revealed that it is phylogenetically related to the AcrA of Staphylococcus sp. and a RND transporter of Vibrio sp. The widespread presence of MR-CoNS is a cause for serious concern as sewage treatment plants are reservoirs for the spread of antibiotic resistance.
| Published in | Pharmaceutical Science and Technology (Volume 5, Issue 1) |
| DOI | 10.11648/j.pst.20210501.13 |
| Page(s) | 14-23 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2021. Published by Science Publishing Group |
Coagulase Negative Staphylococcus, Efflux Proteins, Multiple Drug Resistant Organism, Phylogenetic Analysis
| [1] | Mangi, Shahida & Hussain, Anwar & Qazi, Muneer & Kanhar, Nisar. (2016). Incidence of multidrug-resistant coagulase-negative Staphylococci in clinical samples of Tertiary care hospitals of Khairpur and Sukkur cities of Pakistan. International Journal of Biosciences. 9. 261-269. 10.12692/ijb/9.4.261-269. |
| [2] | Abulreesh, H. H. (2011). Multidrug-resistant staphylococci in the environment. In International Conference on Biotechnology and Environment Management (Vol. 18, pp. 1-6). |
| [3] | Jevons, M. P., & Parker, M. T. (1964). The evolution of new hospital strains of Staphylococcus aureus. Journal of clinical pathology, 17 3), 243–250. https://doi.org/10.1136/jcp.17.3.243. |
| [4] | Gopalakrishnan, R., & Sureshkumar, D. (2010). Changing trends in antimicrobial susceptibility and hospital-acquired infections over an 8 years period in a tertiary care hospital in relation to introduction of an infection control programme. The Journal of the Association of Physicians of India, 58 Suppl, 25–31. |
| [5] | Patel, A. K., Patel, K. K., Patel, K. R., Shah, S., & Dileep, P. (2010). Time trends in the epidemiology of microbial infections at a tertiary care center in west India over last 5 years. J Assoc Physicians India, 58 (58), 37-40. |
| [6] | D'Souza, N., Rodrigues, C., & Mehta, A. (2010). Molecular characterization of methicillin-resistant Staphylococcus aureus with emergence of epidemic clones of sequence type (ST) 22 and ST 772 in Mumbai, India. Journal of clinical microbiology, 48 (5), 1806–1811. https://doi.org/10.1128/JCM.01867-09. |
| [7] | Rathanin Seng, U. L. (2017). High prevalence of methicillin-resistant coagulase-negative staphylococci isolated from a university environment in Thailand. International Microbiology, 20 (2), 65-73. doi: 10.2436/20.1501.01.286. |
| [8] | CDC, Antibiotic/Antimicrobial resistance report in US (2019), Retrieved from; https://www.cdc.gov/drugresistance/index.html. |
| [9] | Martinez, J. L. (2006). Role of non-clinical environments in the selection of virulence and antibiotic resistance determinants in pathogenic bacteria. J. Biol. Sci, 6 (1), 1-8. |
| [10] | Kümmerer, K., Al-Ahmad, A., & Mersch-Sundermann, V. (2000). Biodegradability of some antibiotics, elimination of the genotoxicity and affection of wastewater bacteria in a simple test. Chemosphere, 40 (7), 701-710. |
| [11] | Watkinson, Andrew & Murby, E. J. & Kolpin, Dana & Costanzo, Simon. (2009). The occurrence of antibiotics in an urban watershed: From wastewater to drinking water. The Science of the total environment. 407. 2711-23. 10.1016/j.scitotenv.2008.11.059. |
| [12] | Volkmann, H., Schwartz, T., Bischoff, P., Kirchen, S. and Obst, U. (2004) Detection of clinically relevant antibiotic resistance genes in municipal wastewater using real-ime PCR (Taqman). J Microbiol Methods 56, 277–286. |
| [13] | Börjesson, S., Matussek, A., Melin, S., Löfgren, S., & Lindgren, P. E. (2010). Methicillin-resistant Staphylococcus aureus (MRSA) in municipal wastewater: an uncharted threat?. Journal of applied microbiology, 108 (4), 1244–1251. https://doi.org/10.1111/j.1365-2672.2009.04515.x. |
| [14] | Cyprowski M, Szarapińska-Kwaszewska J, Dudkiewicz B, Krajewski JA, Szadkowska-Stańczyk I (2005) Exposure assessment to harmful agents in workplaces in sewage plant workers. Med Pr 56 (3): 213–222. |
| [15] | Gerardi MH, Zimmerman MC (2005) Wastewater pathogens. Wiley, New Jersey. |
| [16] | Becker, K., Heilmann, C., & Peters, G. (2014). Coagulase-negative staphylococci. Clinical microbiology reviews, 27 (4), 870-926. |
| [17] | Longauerova, A. (2006). Coagulase negative Staphylococci and their participation in pathogenesis of human infections. Bratislavské lekárske listy. 107. 448-52. |
| [18] | Kondapaneni, Saileela & Vanaparthi, Nagaraju & Anukolu, Ravishankarreddy & Kumar, A. N. (2017). Biochemical Characterization and Antibiotic Susceptibility of Coagulase Negative Staphylococci Isolates from Exudates and Body Fluids. Indian Journal of Mednodent and Allied Sciences. 5. 10.5958/2347-6206.2017.00012.7. |
| [19] | Dinges, M. M.; Orwin, P. M.; Schlievert, P. M. Exotoxins of Staphylococcus aureus. Clin. Microbiol. Rev. 2000, 13, 16–34. |
| [20] | Cotar, Ani & Chifiriuc, Mariana & Dinu, Sorin & Bucur, Marcela & Iordache (Curutiu), Carmen & Banu, Otilia & Dracea, Olguta & Larion, Cristina & Lazar, Veronica. (2010). Screening of Molecular Virulence Markers in Staphylococcus aureus and Pseudomonas aeruginosa Strains Isolated from Clinical Infections. International journal of molecular sciences. 11. 5273-91. 10.3390/ijms11125273. |
| [21] | Lakshmi, H. P., Prasad, U. V., Yeswanth, S., Swarupa, V., Prasad, O. H., Narasu, M. L., & Sarma, P. V. (2013). Molecular characterization of α-amylase from Staphylococcus aureus. Bioinformation, 9 (6), 281–285. https://doi.org/10.6026/97320630009281. |
| [22] | Moraveji, Z., Tabatabaei, M., Shirzad Aski, H., & Khoshbakht, R. (2014). Characterization of hemolysins of Staphylococcus strains isolated from human and bovine, southern Iran. Iranian journal of veterinary research, 15 (4), 326–330. |
| [23] | Kresken M, Hafner D. 1999. Drug resistance among clinical isolates of frequently encountered bacterial species in central Europe during 1975–1995. Infection 27 (Suppl 2): S2–S8. doi: 10.1007/BF02561661. |
| [24] | Hamilton-Miller JM, Iliffe A. 1985. Antimicrobial resistance in coagulase-negative staphylococci. J. Med. Microbiol. 19: 217–226. doi: 10.1099/00222615-19-2-217. |
| [25] | Guggenheim M, Zbinden R, Handschin AE, Gohritz A, Altintas MA, Giovanoli P. 2009. Changes in bacterial isolates from burn wounds and their antibiograms: a 20-year study (1986-2005). Burns 35: 553–560. doi: 10.1016/j.burns.2008.09.004. |
| [26] | Jones ME, Karlowsky JA, Draghi DC, Thornsberry C, Sahm DF, Nathwani D. 2003. Epidemiology and antibiotic susceptibility of bacteria causing skin and soft tissue infections in the U.S.A. and Europe: a guide to appropriate antimicrobial therapy. Int. J. Antimicrob. Agents 22: 406–419. doi: 10.1016/S0924-8579(03)00154-7. |
| [27] | Tesch W, Ryffel C, Strässle A, Kayser FH, Berger-Bächi B. 1990. Evidence of a novel staphylococcal mec-encoded element (mecR) controlling expression of penicillin-binding protein 2′. Antimicrob. Agents Chemother. 34: 1703–1706. doi: 10.1128/AAC.34.9.1703. |
| [28] | Hiramatsu K, Katayama Y, Yuzawa H, Ito T. 2002. Molecular genetics of methicillin-resistant Staphylococcus aureus. Int. J. Med. Microbiol. 292: 67–74. doi: 10.1078/1438-4221-00192. |
| [29] | Virdis, S., Scarano, C., Cossu, F., Spanu, V., Spanu, C., & De Santis, E. P. (2010). Antibiotic Resistance in Staphylococcus aureus and Coagulase Negative Staphylococci Isolated from Goats with Subclinical Mastitis. Veterinary medicine international, 2010, 517060. https://doi.org/10.4061/2010/517060. |
| [30] | Pinna A, Zanetti S, Sotgiu M, et al., Identification and antibiotic susceptibility of coagulase negative staphylococci isolated in corneal/external infections. British Journal of Ophthalmology 1999; 83: 771-773. |
| [31] | Archer GL, Climo MW. Antimicrobial susceptibility of coagulase-negative staphylococci. Antimicrob Agents Chemother 1994; 38: 2231–7. |
| [32] | Soparkar, K., Kinana, A. D., Weeks, J. W., Morrison, K. D., Nikaido, H., & Misra, R. (2015). Reversal of the Drug Binding Pocket Defects of the AcrB Multidrug Efflux Pump Protein of Escherichia coli. Journal of bacteriology, 197 (20), 3255–3264. https://doi.org/10.1128/JB.00547-15. |
| [33] | Otto, M. (2013). Coagulase-negative staphylococci as reservoirs of genes facilitating MRSA infection. Bioessays 35, 4-11. |
| [34] | Xu Z, M. H. (2015). Antibiotic resistance and mecA characterization of coagulase-negative staphylococci isolated from three hotels in London, UK. Front Microbiol, 6, 947. doi: 10.3389/fmicb.2015.00947. |
| [35] | Zhang M, O. M. (2011). Prevalence of antiseptic-resistance genes in Staphylococcus aureus and coagulase-negative staphylococci colonising nurses and the general population in Hong Kong. J Hosp Infect., 78 (2), 113-117. doi: 10.1016/j.jhin.2011.02.018. |
| [36] | Li, X. Z., & Nikaido, H. (2009). Efflux-mediated drug resistance in bacteria: an update. Drugs, 69 (12), 1555–1623. https://doi.org/10.2165/11317030-000000000-00000. |
APA Style
Kannan Balachander, Deborah Gnana Selvam Alexander. (2021). Antibiotic Resistance, Virulence Factors and Phylogenetic Analysis of Efflux Proteins of Coagulase Negative Staphylococcus Isolates from Sewage Samples. Pharmaceutical Science and Technology, 5(1), 14-23. https://doi.org/10.11648/j.pst.20210501.13
ACS Style
Kannan Balachander; Deborah Gnana Selvam Alexander. Antibiotic Resistance, Virulence Factors and Phylogenetic Analysis of Efflux Proteins of Coagulase Negative Staphylococcus Isolates from Sewage Samples. Pharm. Sci. Technol. 2021, 5(1), 14-23. doi: 10.11648/j.pst.20210501.13
AMA Style
Kannan Balachander, Deborah Gnana Selvam Alexander. Antibiotic Resistance, Virulence Factors and Phylogenetic Analysis of Efflux Proteins of Coagulase Negative Staphylococcus Isolates from Sewage Samples. Pharm Sci Technol. 2021;5(1):14-23. doi: 10.11648/j.pst.20210501.13
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Download@article{10.11648/j.pst.20210501.13,
author = {Kannan Balachander and Deborah Gnana Selvam Alexander},
title = {Antibiotic Resistance, Virulence Factors and Phylogenetic Analysis of Efflux Proteins of Coagulase Negative Staphylococcus Isolates from Sewage Samples},
journal = {Pharmaceutical Science and Technology},
volume = {5},
number = {1},
pages = {14-23},
doi = {10.11648/j.pst.20210501.13},
url = {https://doi.org/10.11648/j.pst.20210501.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.pst.20210501.13},
abstract = {Multidrug-resistant pathogens are now emergent worldwide and pose a serious threat to disease prevention and treatment. The World Health Organization declared anti-microbial resistance as a major peril to human beings. Staphylococcus is part of the normal microbiome of humans and are responsible for opportunistic infections. Like S. aureus, Coagulase-negative staphylococcus are also clinically important as the causal agents of severe diseases, nosocomial infections, catheter-associated infection, bacteremia, septicemia. Methicillin resistant Staphylococci species are widely known and methicillin resistant CoNS, known as MR-CoNS, have been found to carry the mecA gene. Various virulence factors help these organisms in their pathogenicity and antibiotic-resistance is aided by the presence of efflux pumps. In our study, we have isolated 45 isolates from sewage water which were tentatively identified as CoNS based on biochemical characteristics. Then virulence and antibiotics susceptibility patterns were screened by standard protocols. Multiple drug resistant CoNS were found in our study and in silico analysis of an annotated protein sequence of the efflux pump SepA of Pseudomonas putida was carried out. Sequence analysis of the efflux protein gene revealed that it is phylogenetically related to the AcrA of Staphylococcus sp. and a RND transporter of Vibrio sp. The widespread presence of MR-CoNS is a cause for serious concern as sewage treatment plants are reservoirs for the spread of antibiotic resistance.},
year = {2021}
}
TY - JOUR T1 - Antibiotic Resistance, Virulence Factors and Phylogenetic Analysis of Efflux Proteins of Coagulase Negative Staphylococcus Isolates from Sewage Samples AU - Kannan Balachander AU - Deborah Gnana Selvam Alexander Y1 - 2021/05/26 PY - 2021 N1 - https://doi.org/10.11648/j.pst.20210501.13 DO - 10.11648/j.pst.20210501.13 T2 - Pharmaceutical Science and Technology JF - Pharmaceutical Science and Technology JO - Pharmaceutical Science and Technology SP - 14 EP - 23 PB - Science Publishing Group SN - 2640-4540 UR - https://doi.org/10.11648/j.pst.20210501.13 AB - Multidrug-resistant pathogens are now emergent worldwide and pose a serious threat to disease prevention and treatment. The World Health Organization declared anti-microbial resistance as a major peril to human beings. Staphylococcus is part of the normal microbiome of humans and are responsible for opportunistic infections. Like S. aureus, Coagulase-negative staphylococcus are also clinically important as the causal agents of severe diseases, nosocomial infections, catheter-associated infection, bacteremia, septicemia. Methicillin resistant Staphylococci species are widely known and methicillin resistant CoNS, known as MR-CoNS, have been found to carry the mecA gene. Various virulence factors help these organisms in their pathogenicity and antibiotic-resistance is aided by the presence of efflux pumps. In our study, we have isolated 45 isolates from sewage water which were tentatively identified as CoNS based on biochemical characteristics. Then virulence and antibiotics susceptibility patterns were screened by standard protocols. Multiple drug resistant CoNS were found in our study and in silico analysis of an annotated protein sequence of the efflux pump SepA of Pseudomonas putida was carried out. Sequence analysis of the efflux protein gene revealed that it is phylogenetically related to the AcrA of Staphylococcus sp. and a RND transporter of Vibrio sp. The widespread presence of MR-CoNS is a cause for serious concern as sewage treatment plants are reservoirs for the spread of antibiotic resistance. VL - 5 IS - 1 ER -
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