Point of Care Blood Gas Analyser Verification at the Largest Academic Hospital in Southern Hemisphere Revived by Coronavirus Calls for Tests to Be Verified

Ernest Philani Buthelezi; Edwin Rampota; Matumi Mphogo; Florence Marule; Donald Moshen Tanyanyiwa

doi:doi:10.11648/j.plm.20200402.11

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Point of Care Blood Gas Analyser Verification at the Largest Academic Hospital in Southern Hemisphere Revived by Coronavirus Calls for Tests to Be Verified

Ernest Philani Buthelezi, Edwin Rampota, Matumi Mphogo, Florence Marule, Donald Moshen Tanyanyiwa

Published in Pathology and Laboratory Medicine (Volume 4, Issue 2)

Received: 19 April 2020 Accepted: 9 May 2020 Published: 15 June 2020

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Abstract

Introduction: At no point in the history of medicine has the importance of point-of-care testing (POCT) devices, tests and methods verification and utilisation been recognised and accepted than during the coronavirus pandemic. Blood gas analysers offer the best form of POCT and this has been demonstrated in the management of coronavirus patients admitted to hospital intensive care units with respiratory challenges. The new analysers were evaluated in compliance with the Clinical Laboratory Improvement Amendments (CLIA) regulation. The new method must be evaluated by comparison to the central laboratory or the outgoing analyser. The study was undertaken to verify if the new instrument, OMNI S b221 matches the well-established analysers currently in use at the hospital. Objectives: To evaluate method performance of pH, the partial pressure of carbon dioxide (pCO₂), the partial pressure of oxygen (pO₂), glucose, lactate, and electrolytes on OMNI S b221 blood gas analyser. Materials and Methods: To analyse the method performance of the pH, pO₂, pCO₂, glucose, lactate and electrolytes (Na⁺, K⁺ Ca⁺⁺) on OMNI S b221 blood gas analyser 40 samples run on OMNI S b221 blood gas analyser were compared with the results obtained from ABL700, GEM PREMIER for all the parameters and the Roche Hitachi 917 analyser to compare two electrolytes (Na⁺ and K⁺). The correlation coefficient was calculated for the results obtained from each paired set of instruments (ABL 700 vs GEM PREMIER and ABL 700 vs OMNI S b221). Comparability between analyser methods performance was determined using linear regression analysis. Results: The correlation coefficient between the paired analytical platforms were all between 0.81 and 0.99, demonstrating a strong linear relationship. The coefficient of variation for all the parameters were less than 2 also demonstrating good precision. Conclusion: OMNI S b221 blood gas analyser correlated well with the outgoing ABL 700, GEM PREMIER blood gas analyser and the central laboratory analyser, Roche Hitachi 917 and therefore, verified as a good platform to run arterial blood gas samples.

Published in	Pathology and Laboratory Medicine (Volume 4, Issue 2)
DOI	10.11648/j.plm.20200402.11
Page(s)	27-36
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Point of Care Testing, Central Laboratory, Electrolytes, Arterial Blood, Regression Analysis, Coefficient of Variation

References

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APA Style

Ernest Philani Buthelezi, Edwin Rampota, Matumi Mphogo, Florence Marule, Donald Moshen Tanyanyiwa. (2020). Point of Care Blood Gas Analyser Verification at the Largest Academic Hospital in Southern Hemisphere Revived by Coronavirus Calls for Tests to Be Verified. Pathology and Laboratory Medicine, 4(2), 27-36. https://doi.org/10.11648/j.plm.20200402.11

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ACS Style

Ernest Philani Buthelezi; Edwin Rampota; Matumi Mphogo; Florence Marule; Donald Moshen Tanyanyiwa. Point of Care Blood Gas Analyser Verification at the Largest Academic Hospital in Southern Hemisphere Revived by Coronavirus Calls for Tests to Be Verified. Pathol. Lab. Med. 2020, 4(2), 27-36. doi: 10.11648/j.plm.20200402.11

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AMA Style

Ernest Philani Buthelezi, Edwin Rampota, Matumi Mphogo, Florence Marule, Donald Moshen Tanyanyiwa. Point of Care Blood Gas Analyser Verification at the Largest Academic Hospital in Southern Hemisphere Revived by Coronavirus Calls for Tests to Be Verified. Pathol Lab Med. 2020;4(2):27-36. doi: 10.11648/j.plm.20200402.11

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@article{10.11648/j.plm.20200402.11,
  author = {Ernest Philani Buthelezi and Edwin Rampota and Matumi Mphogo and Florence Marule and Donald Moshen Tanyanyiwa},
  title = {Point of Care Blood Gas Analyser Verification at the Largest Academic Hospital in Southern Hemisphere Revived by Coronavirus Calls for Tests to Be Verified},
  journal = {Pathology and Laboratory Medicine},
  volume = {4},
  number = {2},
  pages = {27-36},
  doi = {10.11648/j.plm.20200402.11},
  url = {https://doi.org/10.11648/j.plm.20200402.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plm.20200402.11},
  abstract = {Introduction: At no point in the history of medicine has the importance of point-of-care testing (POCT) devices, tests and methods verification and utilisation been recognised and accepted than during the coronavirus pandemic. Blood gas analysers offer the best form of POCT and this has been demonstrated in the management of coronavirus patients admitted to hospital intensive care units with respiratory challenges. The new analysers were evaluated in compliance with the Clinical Laboratory Improvement Amendments (CLIA) regulation. The new method must be evaluated by comparison to the central laboratory or the outgoing analyser. The study was undertaken to verify if the new instrument, OMNI S b221 matches the well-established analysers currently in use at the hospital. Objectives: To evaluate method performance of pH, the partial pressure of carbon dioxide (pCO2), the partial pressure of oxygen (pO2), glucose, lactate, and electrolytes on OMNI S b221 blood gas analyser. Materials and Methods: To analyse the method performance of the pH, pO2, pCO2, glucose, lactate and electrolytes (Na+, K+ Ca++) on OMNI S b221 blood gas analyser 40 samples run on OMNI S b221 blood gas analyser were compared with the results obtained from ABL700, GEM PREMIER for all the parameters and the Roche Hitachi 917 analyser to compare two electrolytes (Na+ and K+). The correlation coefficient was calculated for the results obtained from each paired set of instruments (ABL 700 vs GEM PREMIER and ABL 700 vs OMNI S b221). Comparability between analyser methods performance was determined using linear regression analysis. Results: The correlation coefficient between the paired analytical platforms were all between 0.81 and 0.99, demonstrating a strong linear relationship. The coefficient of variation for all the parameters were less than 2 also demonstrating good precision. Conclusion: OMNI S b221 blood gas analyser correlated well with the outgoing ABL 700, GEM PREMIER blood gas analyser and the central laboratory analyser, Roche Hitachi 917 and therefore, verified as a good platform to run arterial blood gas samples.},
 year = {2020}
}

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TY  - JOUR
T1  - Point of Care Blood Gas Analyser Verification at the Largest Academic Hospital in Southern Hemisphere Revived by Coronavirus Calls for Tests to Be Verified
AU  - Ernest Philani Buthelezi
AU  - Edwin Rampota
AU  - Matumi Mphogo
AU  - Florence Marule
AU  - Donald Moshen Tanyanyiwa
Y1  - 2020/06/15
PY  - 2020
N1  - https://doi.org/10.11648/j.plm.20200402.11
DO  - 10.11648/j.plm.20200402.11
T2  - Pathology and Laboratory Medicine
JF  - Pathology and Laboratory Medicine
JO  - Pathology and Laboratory Medicine
SP  - 27
EP  - 36
PB  - Science Publishing Group
SN  - 2640-4478
UR  - https://doi.org/10.11648/j.plm.20200402.11
AB  - Introduction: At no point in the history of medicine has the importance of point-of-care testing (POCT) devices, tests and methods verification and utilisation been recognised and accepted than during the coronavirus pandemic. Blood gas analysers offer the best form of POCT and this has been demonstrated in the management of coronavirus patients admitted to hospital intensive care units with respiratory challenges. The new analysers were evaluated in compliance with the Clinical Laboratory Improvement Amendments (CLIA) regulation. The new method must be evaluated by comparison to the central laboratory or the outgoing analyser. The study was undertaken to verify if the new instrument, OMNI S b221 matches the well-established analysers currently in use at the hospital. Objectives: To evaluate method performance of pH, the partial pressure of carbon dioxide (pCO2), the partial pressure of oxygen (pO2), glucose, lactate, and electrolytes on OMNI S b221 blood gas analyser. Materials and Methods: To analyse the method performance of the pH, pO2, pCO2, glucose, lactate and electrolytes (Na+, K+ Ca++) on OMNI S b221 blood gas analyser 40 samples run on OMNI S b221 blood gas analyser were compared with the results obtained from ABL700, GEM PREMIER for all the parameters and the Roche Hitachi 917 analyser to compare two electrolytes (Na+ and K+). The correlation coefficient was calculated for the results obtained from each paired set of instruments (ABL 700 vs GEM PREMIER and ABL 700 vs OMNI S b221). Comparability between analyser methods performance was determined using linear regression analysis. Results: The correlation coefficient between the paired analytical platforms were all between 0.81 and 0.99, demonstrating a strong linear relationship. The coefficient of variation for all the parameters were less than 2 also demonstrating good precision. Conclusion: OMNI S b221 blood gas analyser correlated well with the outgoing ABL 700, GEM PREMIER blood gas analyser and the central laboratory analyser, Roche Hitachi 917 and therefore, verified as a good platform to run arterial blood gas samples.
VL  - 4
IS  - 2
ER  -

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Author Information

Ernest Philani Buthelezi

Department of Health, Laboratory and Blood Services, Johannesburg, South Africa
Edwin Rampota

Department of Chemical Pathology National Health Laboratory Service, Johannesburg, South Africa
Matumi Mphogo

Department of Chemical Pathology National Health Laboratory Service, Johannesburg, South Africa
Florence Marule

Department of Chemical Pathology National Health Laboratory Service, Johannesburg, South Africa
Donald Moshen Tanyanyiwa

Department of Chemical Pathology, University of the Witwatersrand, Johannesburg, South Africa

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APA Style

Ernest Philani Buthelezi, Edwin Rampota, Matumi Mphogo, Florence Marule, Donald Moshen Tanyanyiwa. (2020). Point of Care Blood Gas Analyser Verification at the Largest Academic Hospital in Southern Hemisphere Revived by Coronavirus Calls for Tests to Be Verified. Pathology and Laboratory Medicine, 4(2), 27-36. https://doi.org/10.11648/j.plm.20200402.11

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ACS Style

Ernest Philani Buthelezi; Edwin Rampota; Matumi Mphogo; Florence Marule; Donald Moshen Tanyanyiwa. Point of Care Blood Gas Analyser Verification at the Largest Academic Hospital in Southern Hemisphere Revived by Coronavirus Calls for Tests to Be Verified. Pathol. Lab. Med. 2020, 4(2), 27-36. doi: 10.11648/j.plm.20200402.11

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AMA Style

Ernest Philani Buthelezi, Edwin Rampota, Matumi Mphogo, Florence Marule, Donald Moshen Tanyanyiwa. Point of Care Blood Gas Analyser Verification at the Largest Academic Hospital in Southern Hemisphere Revived by Coronavirus Calls for Tests to Be Verified. Pathol Lab Med. 2020;4(2):27-36. doi: 10.11648/j.plm.20200402.11

Copy | Download

@article{10.11648/j.plm.20200402.11,
  author = {Ernest Philani Buthelezi and Edwin Rampota and Matumi Mphogo and Florence Marule and Donald Moshen Tanyanyiwa},
  title = {Point of Care Blood Gas Analyser Verification at the Largest Academic Hospital in Southern Hemisphere Revived by Coronavirus Calls for Tests to Be Verified},
  journal = {Pathology and Laboratory Medicine},
  volume = {4},
  number = {2},
  pages = {27-36},
  doi = {10.11648/j.plm.20200402.11},
  url = {https://doi.org/10.11648/j.plm.20200402.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plm.20200402.11},
  abstract = {Introduction: At no point in the history of medicine has the importance of point-of-care testing (POCT) devices, tests and methods verification and utilisation been recognised and accepted than during the coronavirus pandemic. Blood gas analysers offer the best form of POCT and this has been demonstrated in the management of coronavirus patients admitted to hospital intensive care units with respiratory challenges. The new analysers were evaluated in compliance with the Clinical Laboratory Improvement Amendments (CLIA) regulation. The new method must be evaluated by comparison to the central laboratory or the outgoing analyser. The study was undertaken to verify if the new instrument, OMNI S b221 matches the well-established analysers currently in use at the hospital. Objectives: To evaluate method performance of pH, the partial pressure of carbon dioxide (pCO2), the partial pressure of oxygen (pO2), glucose, lactate, and electrolytes on OMNI S b221 blood gas analyser. Materials and Methods: To analyse the method performance of the pH, pO2, pCO2, glucose, lactate and electrolytes (Na+, K+ Ca++) on OMNI S b221 blood gas analyser 40 samples run on OMNI S b221 blood gas analyser were compared with the results obtained from ABL700, GEM PREMIER for all the parameters and the Roche Hitachi 917 analyser to compare two electrolytes (Na+ and K+). The correlation coefficient was calculated for the results obtained from each paired set of instruments (ABL 700 vs GEM PREMIER and ABL 700 vs OMNI S b221). Comparability between analyser methods performance was determined using linear regression analysis. Results: The correlation coefficient between the paired analytical platforms were all between 0.81 and 0.99, demonstrating a strong linear relationship. The coefficient of variation for all the parameters were less than 2 also demonstrating good precision. Conclusion: OMNI S b221 blood gas analyser correlated well with the outgoing ABL 700, GEM PREMIER blood gas analyser and the central laboratory analyser, Roche Hitachi 917 and therefore, verified as a good platform to run arterial blood gas samples.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Point of Care Blood Gas Analyser Verification at the Largest Academic Hospital in Southern Hemisphere Revived by Coronavirus Calls for Tests to Be Verified
AU  - Ernest Philani Buthelezi
AU  - Edwin Rampota
AU  - Matumi Mphogo
AU  - Florence Marule
AU  - Donald Moshen Tanyanyiwa
Y1  - 2020/06/15
PY  - 2020
N1  - https://doi.org/10.11648/j.plm.20200402.11
DO  - 10.11648/j.plm.20200402.11
T2  - Pathology and Laboratory Medicine
JF  - Pathology and Laboratory Medicine
JO  - Pathology and Laboratory Medicine
SP  - 27
EP  - 36
PB  - Science Publishing Group
SN  - 2640-4478
UR  - https://doi.org/10.11648/j.plm.20200402.11
AB  - Introduction: At no point in the history of medicine has the importance of point-of-care testing (POCT) devices, tests and methods verification and utilisation been recognised and accepted than during the coronavirus pandemic. Blood gas analysers offer the best form of POCT and this has been demonstrated in the management of coronavirus patients admitted to hospital intensive care units with respiratory challenges. The new analysers were evaluated in compliance with the Clinical Laboratory Improvement Amendments (CLIA) regulation. The new method must be evaluated by comparison to the central laboratory or the outgoing analyser. The study was undertaken to verify if the new instrument, OMNI S b221 matches the well-established analysers currently in use at the hospital. Objectives: To evaluate method performance of pH, the partial pressure of carbon dioxide (pCO2), the partial pressure of oxygen (pO2), glucose, lactate, and electrolytes on OMNI S b221 blood gas analyser. Materials and Methods: To analyse the method performance of the pH, pO2, pCO2, glucose, lactate and electrolytes (Na+, K+ Ca++) on OMNI S b221 blood gas analyser 40 samples run on OMNI S b221 blood gas analyser were compared with the results obtained from ABL700, GEM PREMIER for all the parameters and the Roche Hitachi 917 analyser to compare two electrolytes (Na+ and K+). The correlation coefficient was calculated for the results obtained from each paired set of instruments (ABL 700 vs GEM PREMIER and ABL 700 vs OMNI S b221). Comparability between analyser methods performance was determined using linear regression analysis. Results: The correlation coefficient between the paired analytical platforms were all between 0.81 and 0.99, demonstrating a strong linear relationship. The coefficient of variation for all the parameters were less than 2 also demonstrating good precision. Conclusion: OMNI S b221 blood gas analyser correlated well with the outgoing ABL 700, GEM PREMIER blood gas analyser and the central laboratory analyser, Roche Hitachi 917 and therefore, verified as a good platform to run arterial blood gas samples.
VL  - 4
IS  - 2
ER  -

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