Although leather industry plays a significant role in the global economy, it has been categorized as one of highly polluting industries due to its generation of huge amount liquid and solid wastes. Large amount of solid and liquid wastes is generated from tanning industries during leather manufacturing process. These wastes bring about various problems to the environment and human health. Tannery wastes contain significant amount of raw skin wastes that are produced in hide/leather trimming process. The recycling and use of these raw skin/hide wastes (trimming wastes) is very important from environmental as well as economic point of views. The aim of this work was to investigate production of collagen from sheep skin trimming waste. The trimming waste was first pre-treated and then acetic acid hydrolysed to extract collagen. The extracted collagen was characterized using Fourier Transform Infrared (FTIR), UV-spectrometer and Scanning Electron Microscope (SEM). Response Surface Methodology (RSM) was used to optimize collagen extraction parameters (acetic acid, mixing ratio and extraction time). A three-factor, three-level Box-Behnken Design (BBD) based RSM was applied to determine the optimal conditions to obtain maximum collagen yield. A maximum collagen yield of 25.75% was obtained at optimum conditions (acetic acid concentration of 0.54 M, skin to acetic acid ratio of 0.03 g/ml and extraction time 3.33 day) using the RSM. Under these optimum conditions, experimental determined collagen yield of acid-soluble collagen was 25.85% which is in good agreement with the predicted value.
| Published in | Journal of Energy, Environmental & Chemical Engineering (Volume 7, Issue 1) |
| DOI | 10.11648/j.jeece.20220701.12 |
| Page(s) | 9-18 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Collagen, Extraction, Sheep Skin, Trimming Waste, RSM
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APA Style
Muleta Ali Wakjira, Girma Gonfa Hunde, Muluneh Ayalew Balcha, Nedumaran Balasubramanian. (2022). Optimization of Collagen Extraction from Sheep Raw Trimming Wastes Using Acid Hydrolysis. Journal of Energy, Environmental & Chemical Engineering, 7(1), 9-18. https://doi.org/10.11648/j.jeece.20220701.12
ACS Style
Muleta Ali Wakjira; Girma Gonfa Hunde; Muluneh Ayalew Balcha; Nedumaran Balasubramanian. Optimization of Collagen Extraction from Sheep Raw Trimming Wastes Using Acid Hydrolysis. J. Energy Environ. Chem. Eng. 2022, 7(1), 9-18. doi: 10.11648/j.jeece.20220701.12
AMA Style
Muleta Ali Wakjira, Girma Gonfa Hunde, Muluneh Ayalew Balcha, Nedumaran Balasubramanian. Optimization of Collagen Extraction from Sheep Raw Trimming Wastes Using Acid Hydrolysis. J Energy Environ Chem Eng. 2022;7(1):9-18. doi: 10.11648/j.jeece.20220701.12
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Download@article{10.11648/j.jeece.20220701.12,
author = {Muleta Ali Wakjira and Girma Gonfa Hunde and Muluneh Ayalew Balcha and Nedumaran Balasubramanian},
title = {Optimization of Collagen Extraction from Sheep Raw Trimming Wastes Using Acid Hydrolysis},
journal = {Journal of Energy, Environmental & Chemical Engineering},
volume = {7},
number = {1},
pages = {9-18},
doi = {10.11648/j.jeece.20220701.12},
url = {https://doi.org/10.11648/j.jeece.20220701.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jeece.20220701.12},
abstract = {Although leather industry plays a significant role in the global economy, it has been categorized as one of highly polluting industries due to its generation of huge amount liquid and solid wastes. Large amount of solid and liquid wastes is generated from tanning industries during leather manufacturing process. These wastes bring about various problems to the environment and human health. Tannery wastes contain significant amount of raw skin wastes that are produced in hide/leather trimming process. The recycling and use of these raw skin/hide wastes (trimming wastes) is very important from environmental as well as economic point of views. The aim of this work was to investigate production of collagen from sheep skin trimming waste. The trimming waste was first pre-treated and then acetic acid hydrolysed to extract collagen. The extracted collagen was characterized using Fourier Transform Infrared (FTIR), UV-spectrometer and Scanning Electron Microscope (SEM). Response Surface Methodology (RSM) was used to optimize collagen extraction parameters (acetic acid, mixing ratio and extraction time). A three-factor, three-level Box-Behnken Design (BBD) based RSM was applied to determine the optimal conditions to obtain maximum collagen yield. A maximum collagen yield of 25.75% was obtained at optimum conditions (acetic acid concentration of 0.54 M, skin to acetic acid ratio of 0.03 g/ml and extraction time 3.33 day) using the RSM. Under these optimum conditions, experimental determined collagen yield of acid-soluble collagen was 25.85% which is in good agreement with the predicted value.},
year = {2022}
}
TY - JOUR T1 - Optimization of Collagen Extraction from Sheep Raw Trimming Wastes Using Acid Hydrolysis AU - Muleta Ali Wakjira AU - Girma Gonfa Hunde AU - Muluneh Ayalew Balcha AU - Nedumaran Balasubramanian Y1 - 2022/03/11 PY - 2022 N1 - https://doi.org/10.11648/j.jeece.20220701.12 DO - 10.11648/j.jeece.20220701.12 T2 - Journal of Energy, Environmental & Chemical Engineering JF - Journal of Energy, Environmental & Chemical Engineering JO - Journal of Energy, Environmental & Chemical Engineering SP - 9 EP - 18 PB - Science Publishing Group SN - 2637-434X UR - https://doi.org/10.11648/j.jeece.20220701.12 AB - Although leather industry plays a significant role in the global economy, it has been categorized as one of highly polluting industries due to its generation of huge amount liquid and solid wastes. Large amount of solid and liquid wastes is generated from tanning industries during leather manufacturing process. These wastes bring about various problems to the environment and human health. Tannery wastes contain significant amount of raw skin wastes that are produced in hide/leather trimming process. The recycling and use of these raw skin/hide wastes (trimming wastes) is very important from environmental as well as economic point of views. The aim of this work was to investigate production of collagen from sheep skin trimming waste. The trimming waste was first pre-treated and then acetic acid hydrolysed to extract collagen. The extracted collagen was characterized using Fourier Transform Infrared (FTIR), UV-spectrometer and Scanning Electron Microscope (SEM). Response Surface Methodology (RSM) was used to optimize collagen extraction parameters (acetic acid, mixing ratio and extraction time). A three-factor, three-level Box-Behnken Design (BBD) based RSM was applied to determine the optimal conditions to obtain maximum collagen yield. A maximum collagen yield of 25.75% was obtained at optimum conditions (acetic acid concentration of 0.54 M, skin to acetic acid ratio of 0.03 g/ml and extraction time 3.33 day) using the RSM. Under these optimum conditions, experimental determined collagen yield of acid-soluble collagen was 25.85% which is in good agreement with the predicted value. VL - 7 IS - 1 ER -
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