Corn silk contain inherently substantial flavonoid that contribute to its antioxidant and anticancer activity. The objective of the present study was to fabricate a system for efficient delivery of the anticancer compounds using microencapsulation technique. Methanolic corn silk extract was microencapsulated in the polymer Poly (d,l-lactide-co-glycolide) – PLGA using the solvent extraction method. The physicochemical properties such as size, morphology and physical state of free and encapsulated microparticles were measured by dynamic light scattering, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in vitro release of compounds were studied and quantified using High Performance Liquid Chromatography (HPLC). Spherical and relatively small (d=485.9) polymeric microparticles were obtained containing flavonoids with encapsulation efficiency (EE) of 60.66%. In vitro release profile exhibit a slow, sustained release and follows the first order kinetic with release rate 3.34 x 10-3 m-1s-1. The release characteristics data showed that the drug is released from the microsphere even after 108 h. For in vitro cell-based assays, the MTT cell viability assay was performed on HeLa, NIH 3T3 cell lines while cellular uptake of the drug was studied using fluorescence microscopy. Fluorescence studies confirm drug uptake by the cells within 24 h of treatment. For confirmation of mode of cell death Flow Cytometry and DNA ladder assay was performed. The blank polymeric microparticles were non-toxic to cell while, the drug loaded microparticles exhibit apoptic cell death. Thus an efficient delivery system is achieved after encapsulation, that provides protection and controlled release of the bioactive compounds.
| Published in | Journal of Drug Design and Medicinal Chemistry (Volume 7, Issue 1) |
| DOI | 10.11648/j.jddmc.20210701.13 |
| Page(s) | 12-22 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Anticancer, Flavonoids, PLGA, Microencapsulation, Drug Release, Apoptosis
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APA Style
Prajakta Kulkarni, Madhumita Tawre, Mahesh Dere, Ayesha Khan. (2021). Microencapsulation of Extracts from Corn Hair: A Study on Drug Release and Anticancer Activity. Journal of Drug Design and Medicinal Chemistry, 7(1), 12-22. https://doi.org/10.11648/j.jddmc.20210701.13
ACS Style
Prajakta Kulkarni; Madhumita Tawre; Mahesh Dere; Ayesha Khan. Microencapsulation of Extracts from Corn Hair: A Study on Drug Release and Anticancer Activity. J. Drug Des. Med. Chem. 2021, 7(1), 12-22. doi: 10.11648/j.jddmc.20210701.13
AMA Style
Prajakta Kulkarni, Madhumita Tawre, Mahesh Dere, Ayesha Khan. Microencapsulation of Extracts from Corn Hair: A Study on Drug Release and Anticancer Activity. J Drug Des Med Chem. 2021;7(1):12-22. doi: 10.11648/j.jddmc.20210701.13
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Download@article{10.11648/j.jddmc.20210701.13,
author = {Prajakta Kulkarni and Madhumita Tawre and Mahesh Dere and Ayesha Khan},
title = {Microencapsulation of Extracts from Corn Hair: A Study on Drug Release and Anticancer Activity},
journal = {Journal of Drug Design and Medicinal Chemistry},
volume = {7},
number = {1},
pages = {12-22},
doi = {10.11648/j.jddmc.20210701.13},
url = {https://doi.org/10.11648/j.jddmc.20210701.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jddmc.20210701.13},
abstract = {Corn silk contain inherently substantial flavonoid that contribute to its antioxidant and anticancer activity. The objective of the present study was to fabricate a system for efficient delivery of the anticancer compounds using microencapsulation technique. Methanolic corn silk extract was microencapsulated in the polymer Poly (d,l-lactide-co-glycolide) – PLGA using the solvent extraction method. The physicochemical properties such as size, morphology and physical state of free and encapsulated microparticles were measured by dynamic light scattering, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in vitro release of compounds were studied and quantified using High Performance Liquid Chromatography (HPLC). Spherical and relatively small (d=485.9) polymeric microparticles were obtained containing flavonoids with encapsulation efficiency (EE) of 60.66%. In vitro release profile exhibit a slow, sustained release and follows the first order kinetic with release rate 3.34 x 10-3 m-1s-1. The release characteristics data showed that the drug is released from the microsphere even after 108 h. For in vitro cell-based assays, the MTT cell viability assay was performed on HeLa, NIH 3T3 cell lines while cellular uptake of the drug was studied using fluorescence microscopy. Fluorescence studies confirm drug uptake by the cells within 24 h of treatment. For confirmation of mode of cell death Flow Cytometry and DNA ladder assay was performed. The blank polymeric microparticles were non-toxic to cell while, the drug loaded microparticles exhibit apoptic cell death. Thus an efficient delivery system is achieved after encapsulation, that provides protection and controlled release of the bioactive compounds.},
year = {2021}
}
TY - JOUR T1 - Microencapsulation of Extracts from Corn Hair: A Study on Drug Release and Anticancer Activity AU - Prajakta Kulkarni AU - Madhumita Tawre AU - Mahesh Dere AU - Ayesha Khan Y1 - 2021/03/30 PY - 2021 N1 - https://doi.org/10.11648/j.jddmc.20210701.13 DO - 10.11648/j.jddmc.20210701.13 T2 - Journal of Drug Design and Medicinal Chemistry JF - Journal of Drug Design and Medicinal Chemistry JO - Journal of Drug Design and Medicinal Chemistry SP - 12 EP - 22 PB - Science Publishing Group SN - 2472-3576 UR - https://doi.org/10.11648/j.jddmc.20210701.13 AB - Corn silk contain inherently substantial flavonoid that contribute to its antioxidant and anticancer activity. The objective of the present study was to fabricate a system for efficient delivery of the anticancer compounds using microencapsulation technique. Methanolic corn silk extract was microencapsulated in the polymer Poly (d,l-lactide-co-glycolide) – PLGA using the solvent extraction method. The physicochemical properties such as size, morphology and physical state of free and encapsulated microparticles were measured by dynamic light scattering, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in vitro release of compounds were studied and quantified using High Performance Liquid Chromatography (HPLC). Spherical and relatively small (d=485.9) polymeric microparticles were obtained containing flavonoids with encapsulation efficiency (EE) of 60.66%. In vitro release profile exhibit a slow, sustained release and follows the first order kinetic with release rate 3.34 x 10-3 m-1s-1. The release characteristics data showed that the drug is released from the microsphere even after 108 h. For in vitro cell-based assays, the MTT cell viability assay was performed on HeLa, NIH 3T3 cell lines while cellular uptake of the drug was studied using fluorescence microscopy. Fluorescence studies confirm drug uptake by the cells within 24 h of treatment. For confirmation of mode of cell death Flow Cytometry and DNA ladder assay was performed. The blank polymeric microparticles were non-toxic to cell while, the drug loaded microparticles exhibit apoptic cell death. Thus an efficient delivery system is achieved after encapsulation, that provides protection and controlled release of the bioactive compounds. VL - 7 IS - 1 ER -
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