Influence of Estrogen, Bevacizumab and Other Factors on VEGF Expression in SKBR-3 Breast Cancer Cells

Zinan Wang; Dezhong Zhao; Ruobing Liu; Bin Zheng

doi:doi:10.11648/j.jctr.20180604.11

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Influence of Estrogen, Bevacizumab and Other Factors on VEGF Expression in SKBR-3 Breast Cancer Cells

Zinan Wang, Dezhong Zhao, Ruobing Liu, Bin Zheng

Published in Journal of Cancer Treatment and Research (Volume 6, Issue 4)

Received: 14 November 2018 Accepted: 5 December 2018 Published: 15 January 2019

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Abstract

OBJECTIVE: To investigate expression of VEGF of SKBR-3 breast cancer cells in different estrogen and to investigate the expression of VEGF after Bevacizumab, Trastuzumab, Taxol and Insulin-like growth factor 1 receptor (IGF-1R)antibodies treatment in different estrogen. METHODS: RT-PCR was used to detect the expression of VEGF in SKBR-3 breast cancer cells under different concentrations of estrogen and VEGF monoclonal antibody, HER-2 monoclonal antibody, paclitaxel and IGF-1R antibody. At the same time, ELISA was used to detect the concentration of VEGF protein in the culture supernatant. RESULTS: The expression of VEGF in no estrogen group was 1.0618±0.0085 (VEGF/GAPDH); the expression of VEGF in low concentration group (50pg/L) was 1.0047±0.0061, the difference was statistically significant compared with the no estrogen group (P=0.002); the expression of VEGF in high concentration group (0.2 μg/mL) was 1.0868±0.0135, the difference was statistically significant compared with the no estrogen group (P<0.001). The expression of VEGF were 1.0496±0.0288 and 1.0618±0.0085 in Taxol group and contral Group (F=0.058), the expression of VEGF were 1.0884±0.0036, 1.0618 ±0.0085 in antibody of IGF-1R group and contral group (F=0.073), the expression of VEGF were 0.9887±0.0037, 1.0618±0.0085 in Trastuzumab group and contral group (F=0.075). The expression of VEGF in the paclitaxel group and the control group were 1.0496±0.0288 and 1.0618±0.0085(F=0.058);The expression of VEGF in IGF-1R group and control group were 1.0884±0.0036 and 1.0618±0.0085(F=0.073);The expression of VEGF in the trastuzumab group and the control group were 0.9887±0.0037 and 1.0618±0.0085(F=0.075); There was no significant change in the expression of VEGF in the paclitaxel group, the IGF-1R group antibody and the trastuzumab group with estrogen. The expression of VEGF in the bevacizumab group and the control group were 1.0057±0.0043 and 1.0618±0.0085( F=0.132, P=0.04), and There is no synergistic effect of estrogen and VEGF monoclonal antibody on the expression of VEGF. Conclusion: The influence of different estrogen on VEGF-expression in SKBR-3 breast cancer cells is remarkable. Taxol, antibody of IGF-1R and Trastuzumab do not change the expression of VEGF in SKBR-3 breast cancer cells, and this has no synergistic effect with estrogen.. Bevacizumab increases the expression of VEGF, and this also has no synergistic effect with estrogen.

Published in	Journal of Cancer Treatment and Research (Volume 6, Issue 4)
DOI	10.11648/j.jctr.20180604.11
Page(s)	54-59
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

SKBR-3 Breast Cancer Cell Line, Estrogen, Bevacizumab, Vascular Endothelial Growth Factor, IGF-1R Antibody, Trastuzumab

References

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APA Style

Zinan Wang, Dezhong Zhao, Ruobing Liu, Bin Zheng. (2019). Influence of Estrogen, Bevacizumab and Other Factors on VEGF Expression in SKBR-3 Breast Cancer Cells. Journal of Cancer Treatment and Research, 6(4), 54-59. https://doi.org/10.11648/j.jctr.20180604.11

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ACS Style

Zinan Wang; Dezhong Zhao; Ruobing Liu; Bin Zheng. Influence of Estrogen, Bevacizumab and Other Factors on VEGF Expression in SKBR-3 Breast Cancer Cells. J. Cancer Treat. Res. 2019, 6(4), 54-59. doi: 10.11648/j.jctr.20180604.11

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AMA Style

Zinan Wang, Dezhong Zhao, Ruobing Liu, Bin Zheng. Influence of Estrogen, Bevacizumab and Other Factors on VEGF Expression in SKBR-3 Breast Cancer Cells. J Cancer Treat Res. 2019;6(4):54-59. doi: 10.11648/j.jctr.20180604.11

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@article{10.11648/j.jctr.20180604.11,
  author = {Zinan Wang and Dezhong Zhao and Ruobing Liu and Bin Zheng},
  title = {Influence of Estrogen, Bevacizumab and Other Factors on VEGF Expression in SKBR-3 Breast Cancer Cells},
  journal = {Journal of Cancer Treatment and Research},
  volume = {6},
  number = {4},
  pages = {54-59},
  doi = {10.11648/j.jctr.20180604.11},
  url = {https://doi.org/10.11648/j.jctr.20180604.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jctr.20180604.11},
  abstract = {OBJECTIVE: To investigate expression of VEGF of SKBR-3 breast cancer cells in different estrogen and to investigate the expression of VEGF after Bevacizumab, Trastuzumab, Taxol and Insulin-like growth factor 1 receptor (IGF-1R)antibodies treatment in different estrogen. METHODS: RT-PCR was used to detect the expression of VEGF in SKBR-3 breast cancer cells under different concentrations of estrogen and VEGF monoclonal antibody, HER-2 monoclonal antibody, paclitaxel and IGF-1R antibody. At the same time, ELISA was used to detect the concentration of VEGF protein in the culture supernatant. RESULTS: The expression of VEGF in no estrogen group was 1.0618±0.0085 (VEGF/GAPDH); the expression of VEGF in low concentration group (50pg/L) was 1.0047±0.0061, the difference was statistically significant compared with the no estrogen group (P=0.002); the expression of VEGF in high concentration group (0.2 μg/mL) was 1.0868±0.0135, the difference was statistically significant compared with the no estrogen group (P<0.001). The expression of VEGF were 1.0496±0.0288 and 1.0618±0.0085 in Taxol group and contral Group (F=0.058), the expression of VEGF were 1.0884±0.0036, 1.0618 ±0.0085 in antibody of IGF-1R group and contral group (F=0.073), the expression of VEGF were 0.9887±0.0037, 1.0618±0.0085 in Trastuzumab group and contral group (F=0.075). The expression of VEGF in the paclitaxel group and the control group were 1.0496±0.0288 and 1.0618±0.0085(F=0.058);The expression of VEGF in IGF-1R group and control group were 1.0884±0.0036 and 1.0618±0.0085(F=0.073);The expression of VEGF in the trastuzumab group and the control group were 0.9887±0.0037 and 1.0618±0.0085(F=0.075); There was no significant change in the expression of VEGF in the paclitaxel group, the IGF-1R group antibody and the trastuzumab group with estrogen. The expression of VEGF in the bevacizumab group and the control group were 1.0057±0.0043 and 1.0618±0.0085( F=0.132, P=0.04), and There is no synergistic effect of estrogen and VEGF monoclonal antibody on the expression of VEGF. Conclusion: The influence of different estrogen on VEGF-expression in SKBR-3 breast cancer cells is remarkable. Taxol, antibody of IGF-1R and Trastuzumab do not change the expression of VEGF in SKBR-3 breast cancer cells, and this has no synergistic effect with estrogen.. Bevacizumab increases the expression of VEGF, and this also has no synergistic effect with estrogen.},
 year = {2019}
}

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TY  - JOUR
T1  - Influence of Estrogen, Bevacizumab and Other Factors on VEGF Expression in SKBR-3 Breast Cancer Cells
AU  - Zinan Wang
AU  - Dezhong Zhao
AU  - Ruobing Liu
AU  - Bin Zheng
Y1  - 2019/01/15
PY  - 2019
N1  - https://doi.org/10.11648/j.jctr.20180604.11
DO  - 10.11648/j.jctr.20180604.11
T2  - Journal of Cancer Treatment and Research
JF  - Journal of Cancer Treatment and Research
JO  - Journal of Cancer Treatment and Research
SP  - 54
EP  - 59
PB  - Science Publishing Group
SN  - 2376-7790
UR  - https://doi.org/10.11648/j.jctr.20180604.11
AB  - OBJECTIVE: To investigate expression of VEGF of SKBR-3 breast cancer cells in different estrogen and to investigate the expression of VEGF after Bevacizumab, Trastuzumab, Taxol and Insulin-like growth factor 1 receptor (IGF-1R)antibodies treatment in different estrogen. METHODS: RT-PCR was used to detect the expression of VEGF in SKBR-3 breast cancer cells under different concentrations of estrogen and VEGF monoclonal antibody, HER-2 monoclonal antibody, paclitaxel and IGF-1R antibody. At the same time, ELISA was used to detect the concentration of VEGF protein in the culture supernatant. RESULTS: The expression of VEGF in no estrogen group was 1.0618±0.0085 (VEGF/GAPDH); the expression of VEGF in low concentration group (50pg/L) was 1.0047±0.0061, the difference was statistically significant compared with the no estrogen group (P=0.002); the expression of VEGF in high concentration group (0.2 μg/mL) was 1.0868±0.0135, the difference was statistically significant compared with the no estrogen group (P<0.001). The expression of VEGF were 1.0496±0.0288 and 1.0618±0.0085 in Taxol group and contral Group (F=0.058), the expression of VEGF were 1.0884±0.0036, 1.0618 ±0.0085 in antibody of IGF-1R group and contral group (F=0.073), the expression of VEGF were 0.9887±0.0037, 1.0618±0.0085 in Trastuzumab group and contral group (F=0.075). The expression of VEGF in the paclitaxel group and the control group were 1.0496±0.0288 and 1.0618±0.0085(F=0.058);The expression of VEGF in IGF-1R group and control group were 1.0884±0.0036 and 1.0618±0.0085(F=0.073);The expression of VEGF in the trastuzumab group and the control group were 0.9887±0.0037 and 1.0618±0.0085(F=0.075); There was no significant change in the expression of VEGF in the paclitaxel group, the IGF-1R group antibody and the trastuzumab group with estrogen. The expression of VEGF in the bevacizumab group and the control group were 1.0057±0.0043 and 1.0618±0.0085( F=0.132, P=0.04), and There is no synergistic effect of estrogen and VEGF monoclonal antibody on the expression of VEGF. Conclusion: The influence of different estrogen on VEGF-expression in SKBR-3 breast cancer cells is remarkable. Taxol, antibody of IGF-1R and Trastuzumab do not change the expression of VEGF in SKBR-3 breast cancer cells, and this has no synergistic effect with estrogen.. Bevacizumab increases the expression of VEGF, and this also has no synergistic effect with estrogen.
VL  - 6
IS  - 4
ER  -

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Author Information

Zinan Wang

Department of Breast, Yan’an Hospital of Kunming City, Kunming, China
Dezhong Zhao

Department of Breast, Yan’an Hospital of Kunming City, Kunming, China
Ruobing Liu

Department of Breast, Yan’an Hospital of Kunming City, Kunming, China
Bin Zheng

Department of Breast, Yan’an Hospital of Kunming City, Kunming, China

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APA Style

Zinan Wang, Dezhong Zhao, Ruobing Liu, Bin Zheng. (2019). Influence of Estrogen, Bevacizumab and Other Factors on VEGF Expression in SKBR-3 Breast Cancer Cells. Journal of Cancer Treatment and Research, 6(4), 54-59. https://doi.org/10.11648/j.jctr.20180604.11

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ACS Style

Zinan Wang; Dezhong Zhao; Ruobing Liu; Bin Zheng. Influence of Estrogen, Bevacizumab and Other Factors on VEGF Expression in SKBR-3 Breast Cancer Cells. J. Cancer Treat. Res. 2019, 6(4), 54-59. doi: 10.11648/j.jctr.20180604.11

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AMA Style

Zinan Wang, Dezhong Zhao, Ruobing Liu, Bin Zheng. Influence of Estrogen, Bevacizumab and Other Factors on VEGF Expression in SKBR-3 Breast Cancer Cells. J Cancer Treat Res. 2019;6(4):54-59. doi: 10.11648/j.jctr.20180604.11

Copy | Download

@article{10.11648/j.jctr.20180604.11,
  author = {Zinan Wang and Dezhong Zhao and Ruobing Liu and Bin Zheng},
  title = {Influence of Estrogen, Bevacizumab and Other Factors on VEGF Expression in SKBR-3 Breast Cancer Cells},
  journal = {Journal of Cancer Treatment and Research},
  volume = {6},
  number = {4},
  pages = {54-59},
  doi = {10.11648/j.jctr.20180604.11},
  url = {https://doi.org/10.11648/j.jctr.20180604.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jctr.20180604.11},
  abstract = {OBJECTIVE: To investigate expression of VEGF of SKBR-3 breast cancer cells in different estrogen and to investigate the expression of VEGF after Bevacizumab, Trastuzumab, Taxol and Insulin-like growth factor 1 receptor (IGF-1R)antibodies treatment in different estrogen. METHODS: RT-PCR was used to detect the expression of VEGF in SKBR-3 breast cancer cells under different concentrations of estrogen and VEGF monoclonal antibody, HER-2 monoclonal antibody, paclitaxel and IGF-1R antibody. At the same time, ELISA was used to detect the concentration of VEGF protein in the culture supernatant. RESULTS: The expression of VEGF in no estrogen group was 1.0618±0.0085 (VEGF/GAPDH); the expression of VEGF in low concentration group (50pg/L) was 1.0047±0.0061, the difference was statistically significant compared with the no estrogen group (P=0.002); the expression of VEGF in high concentration group (0.2 μg/mL) was 1.0868±0.0135, the difference was statistically significant compared with the no estrogen group (P<0.001). The expression of VEGF were 1.0496±0.0288 and 1.0618±0.0085 in Taxol group and contral Group (F=0.058), the expression of VEGF were 1.0884±0.0036, 1.0618 ±0.0085 in antibody of IGF-1R group and contral group (F=0.073), the expression of VEGF were 0.9887±0.0037, 1.0618±0.0085 in Trastuzumab group and contral group (F=0.075). The expression of VEGF in the paclitaxel group and the control group were 1.0496±0.0288 and 1.0618±0.0085(F=0.058);The expression of VEGF in IGF-1R group and control group were 1.0884±0.0036 and 1.0618±0.0085(F=0.073);The expression of VEGF in the trastuzumab group and the control group were 0.9887±0.0037 and 1.0618±0.0085(F=0.075); There was no significant change in the expression of VEGF in the paclitaxel group, the IGF-1R group antibody and the trastuzumab group with estrogen. The expression of VEGF in the bevacizumab group and the control group were 1.0057±0.0043 and 1.0618±0.0085( F=0.132, P=0.04), and There is no synergistic effect of estrogen and VEGF monoclonal antibody on the expression of VEGF. Conclusion: The influence of different estrogen on VEGF-expression in SKBR-3 breast cancer cells is remarkable. Taxol, antibody of IGF-1R and Trastuzumab do not change the expression of VEGF in SKBR-3 breast cancer cells, and this has no synergistic effect with estrogen.. Bevacizumab increases the expression of VEGF, and this also has no synergistic effect with estrogen.},
 year = {2019}
}

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TY  - JOUR
T1  - Influence of Estrogen, Bevacizumab and Other Factors on VEGF Expression in SKBR-3 Breast Cancer Cells
AU  - Zinan Wang
AU  - Dezhong Zhao
AU  - Ruobing Liu
AU  - Bin Zheng
Y1  - 2019/01/15
PY  - 2019
N1  - https://doi.org/10.11648/j.jctr.20180604.11
DO  - 10.11648/j.jctr.20180604.11
T2  - Journal of Cancer Treatment and Research
JF  - Journal of Cancer Treatment and Research
JO  - Journal of Cancer Treatment and Research
SP  - 54
EP  - 59
PB  - Science Publishing Group
SN  - 2376-7790
UR  - https://doi.org/10.11648/j.jctr.20180604.11
AB  - OBJECTIVE: To investigate expression of VEGF of SKBR-3 breast cancer cells in different estrogen and to investigate the expression of VEGF after Bevacizumab, Trastuzumab, Taxol and Insulin-like growth factor 1 receptor (IGF-1R)antibodies treatment in different estrogen. METHODS: RT-PCR was used to detect the expression of VEGF in SKBR-3 breast cancer cells under different concentrations of estrogen and VEGF monoclonal antibody, HER-2 monoclonal antibody, paclitaxel and IGF-1R antibody. At the same time, ELISA was used to detect the concentration of VEGF protein in the culture supernatant. RESULTS: The expression of VEGF in no estrogen group was 1.0618±0.0085 (VEGF/GAPDH); the expression of VEGF in low concentration group (50pg/L) was 1.0047±0.0061, the difference was statistically significant compared with the no estrogen group (P=0.002); the expression of VEGF in high concentration group (0.2 μg/mL) was 1.0868±0.0135, the difference was statistically significant compared with the no estrogen group (P<0.001). The expression of VEGF were 1.0496±0.0288 and 1.0618±0.0085 in Taxol group and contral Group (F=0.058), the expression of VEGF were 1.0884±0.0036, 1.0618 ±0.0085 in antibody of IGF-1R group and contral group (F=0.073), the expression of VEGF were 0.9887±0.0037, 1.0618±0.0085 in Trastuzumab group and contral group (F=0.075). The expression of VEGF in the paclitaxel group and the control group were 1.0496±0.0288 and 1.0618±0.0085(F=0.058);The expression of VEGF in IGF-1R group and control group were 1.0884±0.0036 and 1.0618±0.0085(F=0.073);The expression of VEGF in the trastuzumab group and the control group were 0.9887±0.0037 and 1.0618±0.0085(F=0.075); There was no significant change in the expression of VEGF in the paclitaxel group, the IGF-1R group antibody and the trastuzumab group with estrogen. The expression of VEGF in the bevacizumab group and the control group were 1.0057±0.0043 and 1.0618±0.0085( F=0.132, P=0.04), and There is no synergistic effect of estrogen and VEGF monoclonal antibody on the expression of VEGF. Conclusion: The influence of different estrogen on VEGF-expression in SKBR-3 breast cancer cells is remarkable. Taxol, antibody of IGF-1R and Trastuzumab do not change the expression of VEGF in SKBR-3 breast cancer cells, and this has no synergistic effect with estrogen.. Bevacizumab increases the expression of VEGF, and this also has no synergistic effect with estrogen.
VL  - 6
IS  - 4
ER  -

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