Sensitivity of Redox Cycle Enzymes in Substantiating the Pathophysiology of Cataract

Syed Parween Ali; Mohammed Abdul Rasheed; Mohammed Amanullah

doi:doi:10.11648/j.ijovs.20170201.14

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Sensitivity of Redox Cycle Enzymes in Substantiating the Pathophysiology of Cataract

Syed Parween Ali, Mohammed Abdul Rasheed, Mohammed Amanullah

Published in International Journal of Ophthalmology & Visual Science (Volume 2, Issue 1)

Received: 2 February 2017 Accepted: 27 February 2017 Published: 15 March 2017

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Abstract

Oxidative modifications play major role in the formation of cataract. Lens contains several protective mechanisms against oxidizing agents viz. catalase, superoxide dismutase, glutathione reductase, glutathione peroxidase, and ascorbic acid. To explore the oxidative damage that might be occurring with 'Invitro' development of cataracts induced by sugars, H₂O₂ and steroids. We examined redox status of cataractous lenses by analysing enzymatic defence meachanisms. Lenses were exposed to glucose (50 mM) (Group – I); galactose (35 mM) (Group – II) and xylose (30 mM) (Group – III) and maintained at 37°C for 72 hours so as to induce sugar cataract. H₂O₂ cataract was produced by adding 50 mM (Group – IV) and 100 mM (Group – V), solution to culture media (AAH). Steroid cataract was generated by adding a freshly prepared l x 10^-4 M dexamethasone (mw 392.5) (Group – VI) in absolute alcohol to the culture media (AAH) and incubated at 37°C for 72 hours. Subsequent to the development of the cataract, the lenses were homogenized and the specific activity of the enzymes catalase (CAT), glutathione peroxidase and glutathione reductase was assessed. Catalase activity did not show any significant decrease in sugar cataract and steroid cataract but a significant decrease was observed in H₂O₂ cataract. However a significant decrease in GSH-Px and GSH-Rx were found in all the three types of experimental cataract as compared to control lenses.

Published in	International Journal of Ophthalmology & Visual Science (Volume 2, Issue 1)
DOI	10.11648/j.ijovs.20170201.14
Page(s)	15-21
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Cataract, Catalase, Glutathione Reductase, Glutathione Peroxidase, Free Radicals

References

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APA Style

Syed Parween Ali, Mohammed Abdul Rasheed, Mohammed Amanullah. (2017). Sensitivity of Redox Cycle Enzymes in Substantiating the Pathophysiology of Cataract. International Journal of Ophthalmology & Visual Science, 2(1), 15-21. https://doi.org/10.11648/j.ijovs.20170201.14

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ACS Style

Syed Parween Ali; Mohammed Abdul Rasheed; Mohammed Amanullah. Sensitivity of Redox Cycle Enzymes in Substantiating the Pathophysiology of Cataract. Int. J. Ophthalmol. Vis. Sci. 2017, 2(1), 15-21. doi: 10.11648/j.ijovs.20170201.14

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AMA Style

Syed Parween Ali, Mohammed Abdul Rasheed, Mohammed Amanullah. Sensitivity of Redox Cycle Enzymes in Substantiating the Pathophysiology of Cataract. Int J Ophthalmol Vis Sci. 2017;2(1):15-21. doi: 10.11648/j.ijovs.20170201.14

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@article{10.11648/j.ijovs.20170201.14,
  author = {Syed Parween Ali and Mohammed Abdul Rasheed and Mohammed Amanullah},
  title = {Sensitivity of Redox Cycle Enzymes in Substantiating the Pathophysiology of Cataract},
  journal = {International Journal of Ophthalmology & Visual Science},
  volume = {2},
  number = {1},
  pages = {15-21},
  doi = {10.11648/j.ijovs.20170201.14},
  url = {https://doi.org/10.11648/j.ijovs.20170201.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijovs.20170201.14},
  abstract = {Oxidative modifications play major role in the formation of cataract. Lens contains several protective mechanisms against oxidizing agents viz. catalase, superoxide dismutase, glutathione reductase, glutathione peroxidase, and ascorbic acid. To explore the oxidative damage that might be occurring with 'Invitro' development of cataracts induced by sugars, H2O2 and steroids. We examined redox status of cataractous lenses by analysing enzymatic defence meachanisms. Lenses were exposed to glucose (50 mM) (Group – I); galactose (35 mM) (Group – II) and xylose (30 mM) (Group – III) and maintained at 37°C for 72 hours so as to induce sugar cataract. H2O2 cataract was produced by adding 50 mM (Group – IV) and 100 mM (Group – V), solution to culture media (AAH). Steroid cataract was generated by adding a freshly prepared l x 10-4 M dexamethasone (mw 392.5) (Group – VI) in absolute alcohol to the culture media (AAH) and incubated at 37°C for 72 hours. Subsequent to the development of the cataract, the lenses were homogenized and the specific activity of the enzymes catalase (CAT), glutathione peroxidase and glutathione reductase was assessed. Catalase activity did not show any significant decrease in sugar cataract and steroid cataract but a significant decrease was observed in H2O2 cataract. However a significant decrease in GSH-Px and GSH-Rx were found in all the three types of experimental cataract as compared to control lenses.},
 year = {2017}
}

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TY - JOUR
T1 - Sensitivity of Redox Cycle Enzymes in Substantiating the Pathophysiology of Cataract
AU - Syed Parween Ali
AU - Mohammed Abdul Rasheed
AU - Mohammed Amanullah
Y1 - 2017/03/15
PY - 2017
N1 - https://doi.org/10.11648/j.ijovs.20170201.14
DO - 10.11648/j.ijovs.20170201.14
T2 - International Journal of Ophthalmology & Visual Science
JF - International Journal of Ophthalmology & Visual Science
JO - International Journal of Ophthalmology & Visual Science
SP - 15
EP - 21
PB - Science Publishing Group
SN - 2637-3858
UR - https://doi.org/10.11648/j.ijovs.20170201.14
AB - Oxidative modifications play major role in the formation of cataract. Lens contains several protective mechanisms against oxidizing agents viz. catalase, superoxide dismutase, glutathione reductase, glutathione peroxidase, and ascorbic acid. To explore the oxidative damage that might be occurring with 'Invitro' development of cataracts induced by sugars, H2O2 and steroids. We examined redox status of cataractous lenses by analysing enzymatic defence meachanisms. Lenses were exposed to glucose (50 mM) (Group – I); galactose (35 mM) (Group – II) and xylose (30 mM) (Group – III) and maintained at 37°C for 72 hours so as to induce sugar cataract. H2O2 cataract was produced by adding 50 mM (Group – IV) and 100 mM (Group – V), solution to culture media (AAH). Steroid cataract was generated by adding a freshly prepared l x 10-4 M dexamethasone (mw 392.5) (Group – VI) in absolute alcohol to the culture media (AAH) and incubated at 37°C for 72 hours. Subsequent to the development of the cataract, the lenses were homogenized and the specific activity of the enzymes catalase (CAT), glutathione peroxidase and glutathione reductase was assessed. Catalase activity did not show any significant decrease in sugar cataract and steroid cataract but a significant decrease was observed in H2O2 cataract. However a significant decrease in GSH-Px and GSH-Rx were found in all the three types of experimental cataract as compared to control lenses.
VL - 2
IS - 1
ER -

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Author Information

Syed Parween Ali

Department Medical Laboratory Science, College of Applied Medical Sciences, King Khalid University, Abha, Kingdom of Saudi Arabia
Mohammed Abdul Rasheed

Ministry of Interior, Abha, Kingdom of Saudi Arabia
Mohammed Amanullah

Department of Biochemistry, College of Medicine, King Khalid University, Abha, Kingdom of Saudi Arabia

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APA Style

Syed Parween Ali, Mohammed Abdul Rasheed, Mohammed Amanullah. (2017). Sensitivity of Redox Cycle Enzymes in Substantiating the Pathophysiology of Cataract. International Journal of Ophthalmology & Visual Science, 2(1), 15-21. https://doi.org/10.11648/j.ijovs.20170201.14

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ACS Style

Syed Parween Ali; Mohammed Abdul Rasheed; Mohammed Amanullah. Sensitivity of Redox Cycle Enzymes in Substantiating the Pathophysiology of Cataract. Int. J. Ophthalmol. Vis. Sci. 2017, 2(1), 15-21. doi: 10.11648/j.ijovs.20170201.14

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AMA Style

Syed Parween Ali, Mohammed Abdul Rasheed, Mohammed Amanullah. Sensitivity of Redox Cycle Enzymes in Substantiating the Pathophysiology of Cataract. Int J Ophthalmol Vis Sci. 2017;2(1):15-21. doi: 10.11648/j.ijovs.20170201.14

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@article{10.11648/j.ijovs.20170201.14,
  author = {Syed Parween Ali and Mohammed Abdul Rasheed and Mohammed Amanullah},
  title = {Sensitivity of Redox Cycle Enzymes in Substantiating the Pathophysiology of Cataract},
  journal = {International Journal of Ophthalmology & Visual Science},
  volume = {2},
  number = {1},
  pages = {15-21},
  doi = {10.11648/j.ijovs.20170201.14},
  url = {https://doi.org/10.11648/j.ijovs.20170201.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijovs.20170201.14},
  abstract = {Oxidative modifications play major role in the formation of cataract. Lens contains several protective mechanisms against oxidizing agents viz. catalase, superoxide dismutase, glutathione reductase, glutathione peroxidase, and ascorbic acid. To explore the oxidative damage that might be occurring with 'Invitro' development of cataracts induced by sugars, H2O2 and steroids. We examined redox status of cataractous lenses by analysing enzymatic defence meachanisms. Lenses were exposed to glucose (50 mM) (Group – I); galactose (35 mM) (Group – II) and xylose (30 mM) (Group – III) and maintained at 37°C for 72 hours so as to induce sugar cataract. H2O2 cataract was produced by adding 50 mM (Group – IV) and 100 mM (Group – V), solution to culture media (AAH). Steroid cataract was generated by adding a freshly prepared l x 10-4 M dexamethasone (mw 392.5) (Group – VI) in absolute alcohol to the culture media (AAH) and incubated at 37°C for 72 hours. Subsequent to the development of the cataract, the lenses were homogenized and the specific activity of the enzymes catalase (CAT), glutathione peroxidase and glutathione reductase was assessed. Catalase activity did not show any significant decrease in sugar cataract and steroid cataract but a significant decrease was observed in H2O2 cataract. However a significant decrease in GSH-Px and GSH-Rx were found in all the three types of experimental cataract as compared to control lenses.},
 year = {2017}
}

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