The Effects of Aqueous Leave Extract of <i>Moringa oleifera</i> on the Hippocamppal Histology of Aluminium Chloride-Induced Alzheimer’s Disease in Adult Wistar Rats

Ignatius Ikemefuna Ozor; Zita Njideka Agwag; Elizabeth Finbarrs-Bello; Onyinye Mary Ozioko; Uche Sebastine Oziok o; Naza Christian Iruka

doi:doi:10.11648/j.ijnpt.20200602.12

The Effects of Aqueous Leave Extract of Moringa oleifera on the Hippocamppal Histology of Aluminium Chloride-Induced Alzheimer’s Disease in Adult Wistar Rats

Ignatius Ikemefuna Ozor, Zita Njideka Agwag, Elizabeth Finbarrs-Bello, Onyinye Mary Ozioko, Uche Sebastine Oziok o, Naza Christian Iruka

Published in International Journal of Neurologic Physical Therapy (Volume 6, Issue 2)

Received: 30 July 2020 Accepted: 17 August 2020 Published: 10 September 2020

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Abstract

Introduction: Consumption of antioxidant-rich foods and polyphenol treatment can enhance cognitive performance in elderly subjects. Hence, this study was aimed at investigating the effects of aqueous extract of Moringa oleifera leaves on the hippocampus of aluminium chloride-induced alzheimer’s disease in adult wistar rats. Method: Twenty (20) healthy adult male wistar rats (Rattus norvegicus), weighing 180-240g were divided into five (5) groups of four (4) animals each. Group one served as normal control group and was given free access to normal saline for 21days, group 2 was given 400 mg/kg of Moringa extract only for 21 days, group 3 was given 200 mg/kg of AlCl₃ for 21days. Group 4 which served as treatment group was given co-treatment of Moringa extract and AlCl₃ for 21 days while groups 5 which served as standard drug group received co-treatment of Simvastatin and AlCl3 for 21 days. Parameters studied include; hippocampal CA3 histopathology, and CA3 histochemical changes after H&E and Congo red stains. Results: This study revealed that aqueous leaves extract of Moringa oleifera and standard drug-Simvastatin- at both Histopathological and histochemical parameter was effective. Moringa aqueous extracts ameliorated the reported histochemical and histopathological effects of AlCl₃ in treated rats. Conclusion: This study reveals that the tested extracts provided efficiently a neuroprotective effect against Alzheimer’s disease. The Moringa extract group and Simvastatin group showed effectiveness in treatment of neurodegenerative disease. This shows that Moringa extract is an alternative remedy to Simvastatin in the treatment of neurodegenerative disease.

Published in	International Journal of Neurologic Physical Therapy (Volume 6, Issue 2)
DOI	10.11648/j.ijnpt.20200602.12
Page(s)	23-28
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Alzheimer’s Disease, Hippocampus, Moringa oleifera

References

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Ignatius Ikemefuna Ozor, Zita Njideka Agwag, Elizabeth Finbarrs-Bello, Onyinye Mary Ozioko, Uche Sebastine Oziok o, et al. (2020). The Effects of Aqueous Leave Extract of Moringa oleifera on the Hippocamppal Histology of Aluminium Chloride-Induced Alzheimer’s Disease in Adult Wistar Rats. International Journal of Neurologic Physical Therapy, 6(2), 23-28. https://doi.org/10.11648/j.ijnpt.20200602.12

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ACS Style

Ignatius Ikemefuna Ozor; Zita Njideka Agwag; Elizabeth Finbarrs-Bello; Onyinye Mary Ozioko; Uche Sebastine Oziok o, et al. The Effects of Aqueous Leave Extract of Moringa oleifera on the Hippocamppal Histology of Aluminium Chloride-Induced Alzheimer’s Disease in Adult Wistar Rats. Int. J. Neurol. Phys. Ther. 2020, 6(2), 23-28. doi: 10.11648/j.ijnpt.20200602.12

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AMA Style

Ignatius Ikemefuna Ozor, Zita Njideka Agwag, Elizabeth Finbarrs-Bello, Onyinye Mary Ozioko, Uche Sebastine Oziok o, et al. The Effects of Aqueous Leave Extract of Moringa oleifera on the Hippocamppal Histology of Aluminium Chloride-Induced Alzheimer’s Disease in Adult Wistar Rats. Int J Neurol Phys Ther. 2020;6(2):23-28. doi: 10.11648/j.ijnpt.20200602.12

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@article{10.11648/j.ijnpt.20200602.12,
  author = {Ignatius Ikemefuna Ozor and Zita Njideka Agwag and Elizabeth Finbarrs-Bello and Onyinye Mary Ozioko and Uche Sebastine Oziok o and Naza Christian Iruka},
  title = {The Effects of Aqueous Leave Extract of Moringa oleifera on the Hippocamppal Histology of Aluminium Chloride-Induced Alzheimer’s Disease in Adult Wistar Rats},
  journal = {International Journal of Neurologic Physical Therapy},
  volume = {6},
  number = {2},
  pages = {23-28},
  doi = {10.11648/j.ijnpt.20200602.12},
  url = {https://doi.org/10.11648/j.ijnpt.20200602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnpt.20200602.12},
  abstract = {Introduction: Consumption of antioxidant-rich foods and polyphenol treatment can enhance cognitive performance in elderly subjects. Hence, this study was aimed at investigating the effects of aqueous extract of Moringa oleifera leaves on the hippocampus of aluminium chloride-induced alzheimer’s disease in adult wistar rats. Method: Twenty (20) healthy adult male wistar rats (Rattus norvegicus), weighing 180-240g were divided into five (5) groups of four (4) animals each. Group one served as normal control group and was given free access to normal saline for 21days, group 2 was given 400 mg/kg of Moringa extract only for 21 days, group 3 was given 200 mg/kg of AlCl3 for 21days. Group 4 which served as treatment group was given co-treatment of Moringa extract and AlCl3 for 21 days while groups 5 which served as standard drug group received co-treatment of Simvastatin and AlCl3 for 21 days. Parameters studied include; hippocampal CA3 histopathology, and CA3 histochemical changes after H&E and Congo red stains. Results: This study revealed that aqueous leaves extract of Moringa oleifera and standard drug-Simvastatin- at both Histopathological and histochemical parameter was effective. Moringa aqueous extracts ameliorated the reported histochemical and histopathological effects of AlCl3 in treated rats. Conclusion: This study reveals that the tested extracts provided efficiently a neuroprotective effect against Alzheimer’s disease. The Moringa extract group and Simvastatin group showed effectiveness in treatment of neurodegenerative disease. This shows that Moringa extract is an alternative remedy to Simvastatin in the treatment of neurodegenerative disease.},
 year = {2020}
}

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TY  - JOUR
T1  - The Effects of Aqueous Leave Extract of Moringa oleifera on the Hippocamppal Histology of Aluminium Chloride-Induced Alzheimer’s Disease in Adult Wistar Rats
AU  - Ignatius Ikemefuna Ozor
AU  - Zita Njideka Agwag
AU  - Elizabeth Finbarrs-Bello
AU  - Onyinye Mary Ozioko
AU  - Uche Sebastine Oziok o
AU  - Naza Christian Iruka
Y1  - 2020/09/10
PY  - 2020
N1  - https://doi.org/10.11648/j.ijnpt.20200602.12
DO  - 10.11648/j.ijnpt.20200602.12
T2  - International Journal of Neurologic Physical Therapy
JF  - International Journal of Neurologic Physical Therapy
JO  - International Journal of Neurologic Physical Therapy
SP  - 23
EP  - 28
PB  - Science Publishing Group
SN  - 2575-1778
UR  - https://doi.org/10.11648/j.ijnpt.20200602.12
AB  - Introduction: Consumption of antioxidant-rich foods and polyphenol treatment can enhance cognitive performance in elderly subjects. Hence, this study was aimed at investigating the effects of aqueous extract of Moringa oleifera leaves on the hippocampus of aluminium chloride-induced alzheimer’s disease in adult wistar rats. Method: Twenty (20) healthy adult male wistar rats (Rattus norvegicus), weighing 180-240g were divided into five (5) groups of four (4) animals each. Group one served as normal control group and was given free access to normal saline for 21days, group 2 was given 400 mg/kg of Moringa extract only for 21 days, group 3 was given 200 mg/kg of AlCl3 for 21days. Group 4 which served as treatment group was given co-treatment of Moringa extract and AlCl3 for 21 days while groups 5 which served as standard drug group received co-treatment of Simvastatin and AlCl3 for 21 days. Parameters studied include; hippocampal CA3 histopathology, and CA3 histochemical changes after H&E and Congo red stains. Results: This study revealed that aqueous leaves extract of Moringa oleifera and standard drug-Simvastatin- at both Histopathological and histochemical parameter was effective. Moringa aqueous extracts ameliorated the reported histochemical and histopathological effects of AlCl3 in treated rats. Conclusion: This study reveals that the tested extracts provided efficiently a neuroprotective effect against Alzheimer’s disease. The Moringa extract group and Simvastatin group showed effectiveness in treatment of neurodegenerative disease. This shows that Moringa extract is an alternative remedy to Simvastatin in the treatment of neurodegenerative disease.
VL  - 6
IS  - 2
ER  -

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Author Information

Ignatius Ikemefuna Ozor

Department of Anatomy, College of Medicine, Enugu State University of Science and Technology, Enugu, Nigeria
Zita Njideka Agwag

Department of Nursing, Enugu State University of Science and Technology Teaching Hospital, Enugu, Nigeria
Elizabeth Finbarrs-Bello

Department of Anatomy, College of Medicine, Enugu State University of Science and Technology, Enugu, Nigeria
Onyinye Mary Ozioko

Department of Anatomy, College of Medicine, Enugu State University of Science and Technology, Enugu, Nigeria
Uche Sebastine Oziok o

Department of Anatomy, College of Medicine, Enugu State University of Science and Technology, Enugu, Nigeria
Naza Christian Iruka

Department of Anatomy, College of Medicine, Enugu State University of Science and Technology, Enugu, Nigeria

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APA Style

Ignatius Ikemefuna Ozor, Zita Njideka Agwag, Elizabeth Finbarrs-Bello, Onyinye Mary Ozioko, Uche Sebastine Oziok o, et al. (2020). The Effects of Aqueous Leave Extract of Moringa oleifera on the Hippocamppal Histology of Aluminium Chloride-Induced Alzheimer’s Disease in Adult Wistar Rats. International Journal of Neurologic Physical Therapy, 6(2), 23-28. https://doi.org/10.11648/j.ijnpt.20200602.12

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ACS Style

Ignatius Ikemefuna Ozor; Zita Njideka Agwag; Elizabeth Finbarrs-Bello; Onyinye Mary Ozioko; Uche Sebastine Oziok o, et al. The Effects of Aqueous Leave Extract of Moringa oleifera on the Hippocamppal Histology of Aluminium Chloride-Induced Alzheimer’s Disease in Adult Wistar Rats. Int. J. Neurol. Phys. Ther. 2020, 6(2), 23-28. doi: 10.11648/j.ijnpt.20200602.12

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AMA Style

Ignatius Ikemefuna Ozor, Zita Njideka Agwag, Elizabeth Finbarrs-Bello, Onyinye Mary Ozioko, Uche Sebastine Oziok o, et al. The Effects of Aqueous Leave Extract of Moringa oleifera on the Hippocamppal Histology of Aluminium Chloride-Induced Alzheimer’s Disease in Adult Wistar Rats. Int J Neurol Phys Ther. 2020;6(2):23-28. doi: 10.11648/j.ijnpt.20200602.12

Copy | Download

@article{10.11648/j.ijnpt.20200602.12,
  author = {Ignatius Ikemefuna Ozor and Zita Njideka Agwag and Elizabeth Finbarrs-Bello and Onyinye Mary Ozioko and Uche Sebastine Oziok o and Naza Christian Iruka},
  title = {The Effects of Aqueous Leave Extract of Moringa oleifera on the Hippocamppal Histology of Aluminium Chloride-Induced Alzheimer’s Disease in Adult Wistar Rats},
  journal = {International Journal of Neurologic Physical Therapy},
  volume = {6},
  number = {2},
  pages = {23-28},
  doi = {10.11648/j.ijnpt.20200602.12},
  url = {https://doi.org/10.11648/j.ijnpt.20200602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnpt.20200602.12},
  abstract = {Introduction: Consumption of antioxidant-rich foods and polyphenol treatment can enhance cognitive performance in elderly subjects. Hence, this study was aimed at investigating the effects of aqueous extract of Moringa oleifera leaves on the hippocampus of aluminium chloride-induced alzheimer’s disease in adult wistar rats. Method: Twenty (20) healthy adult male wistar rats (Rattus norvegicus), weighing 180-240g were divided into five (5) groups of four (4) animals each. Group one served as normal control group and was given free access to normal saline for 21days, group 2 was given 400 mg/kg of Moringa extract only for 21 days, group 3 was given 200 mg/kg of AlCl3 for 21days. Group 4 which served as treatment group was given co-treatment of Moringa extract and AlCl3 for 21 days while groups 5 which served as standard drug group received co-treatment of Simvastatin and AlCl3 for 21 days. Parameters studied include; hippocampal CA3 histopathology, and CA3 histochemical changes after H&E and Congo red stains. Results: This study revealed that aqueous leaves extract of Moringa oleifera and standard drug-Simvastatin- at both Histopathological and histochemical parameter was effective. Moringa aqueous extracts ameliorated the reported histochemical and histopathological effects of AlCl3 in treated rats. Conclusion: This study reveals that the tested extracts provided efficiently a neuroprotective effect against Alzheimer’s disease. The Moringa extract group and Simvastatin group showed effectiveness in treatment of neurodegenerative disease. This shows that Moringa extract is an alternative remedy to Simvastatin in the treatment of neurodegenerative disease.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - The Effects of Aqueous Leave Extract of Moringa oleifera on the Hippocamppal Histology of Aluminium Chloride-Induced Alzheimer’s Disease in Adult Wistar Rats
AU  - Ignatius Ikemefuna Ozor
AU  - Zita Njideka Agwag
AU  - Elizabeth Finbarrs-Bello
AU  - Onyinye Mary Ozioko
AU  - Uche Sebastine Oziok o
AU  - Naza Christian Iruka
Y1  - 2020/09/10
PY  - 2020
N1  - https://doi.org/10.11648/j.ijnpt.20200602.12
DO  - 10.11648/j.ijnpt.20200602.12
T2  - International Journal of Neurologic Physical Therapy
JF  - International Journal of Neurologic Physical Therapy
JO  - International Journal of Neurologic Physical Therapy
SP  - 23
EP  - 28
PB  - Science Publishing Group
SN  - 2575-1778
UR  - https://doi.org/10.11648/j.ijnpt.20200602.12
AB  - Introduction: Consumption of antioxidant-rich foods and polyphenol treatment can enhance cognitive performance in elderly subjects. Hence, this study was aimed at investigating the effects of aqueous extract of Moringa oleifera leaves on the hippocampus of aluminium chloride-induced alzheimer’s disease in adult wistar rats. Method: Twenty (20) healthy adult male wistar rats (Rattus norvegicus), weighing 180-240g were divided into five (5) groups of four (4) animals each. Group one served as normal control group and was given free access to normal saline for 21days, group 2 was given 400 mg/kg of Moringa extract only for 21 days, group 3 was given 200 mg/kg of AlCl3 for 21days. Group 4 which served as treatment group was given co-treatment of Moringa extract and AlCl3 for 21 days while groups 5 which served as standard drug group received co-treatment of Simvastatin and AlCl3 for 21 days. Parameters studied include; hippocampal CA3 histopathology, and CA3 histochemical changes after H&E and Congo red stains. Results: This study revealed that aqueous leaves extract of Moringa oleifera and standard drug-Simvastatin- at both Histopathological and histochemical parameter was effective. Moringa aqueous extracts ameliorated the reported histochemical and histopathological effects of AlCl3 in treated rats. Conclusion: This study reveals that the tested extracts provided efficiently a neuroprotective effect against Alzheimer’s disease. The Moringa extract group and Simvastatin group showed effectiveness in treatment of neurodegenerative disease. This shows that Moringa extract is an alternative remedy to Simvastatin in the treatment of neurodegenerative disease.
VL  - 6
IS  - 2
ER  -

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