Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods

Biswarup Ghosh; Angelo Lepore; George Smith

doi:doi:10.11648/j.ijgg.20160406.16

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Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods

Biswarup Ghosh, Angelo Lepore, George Smith

Published in International Journal of Genetics and Genomics (Volume 4, Issue 6)

Received: 18 July 2016 Accepted: 29 July 2016 Published: 22 March 2017

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Abstract

Parkinson’s disease (PD) is characterized as a disease of the basal ganglia, with a progressive degeneration of dopaminergic neurons located in the substantia nigra (SN) and projecting to the striatum with subsequently loss of the nigrostriatal circuit. The potential for therapeutic use of cell transplantation for cell replacement has received a great deal of interest. Transplantation with embryonic ventral mesencephalon (VM) is a therapeutic approach for sporadic form of PD. We established unilaterally 6-OHDA lesioned rat model of Parkinson’s disease. Motor behavioral impairment was found compared with normal rat. Embryonic VM tissue was isolated from E 14 (embryonic 14 days) rat brain. We characterized the VM tissue and dissociated cultured dopaminergic cells with tyrosine hydroxylase (TH) immune staining before transplantation in lesioned brain. We observed that the axons of dopaminergic neurons from transplanted VM graft circles round at the site of transplantation in normal adult rat brain. In this paper, we discuss the detailed methodologies which are very useful in preclinical research of cell based therapies for Parkinson’s disease.

Published in	International Journal of Genetics and Genomics (Volume 4, Issue 6)
DOI	10.11648/j.ijgg.20160406.16
Page(s)	79-84
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Transplantation, 6-OHDA, Parkinson’s Disease

References

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APA Style

Biswarup Ghosh, Angelo Lepore, George Smith. (2017). Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods. International Journal of Genetics and Genomics, 4(6), 79-84. https://doi.org/10.11648/j.ijgg.20160406.16

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ACS Style

Biswarup Ghosh; Angelo Lepore; George Smith. Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods. Int. J. Genet. Genomics 2017, 4(6), 79-84. doi: 10.11648/j.ijgg.20160406.16

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AMA Style

Biswarup Ghosh, Angelo Lepore, George Smith. Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods. Int J Genet Genomics. 2017;4(6):79-84. doi: 10.11648/j.ijgg.20160406.16

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@article{10.11648/j.ijgg.20160406.16,
  author = {Biswarup Ghosh and Angelo Lepore and George Smith},
  title = {Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods},
  journal = {International Journal of Genetics and Genomics},
  volume = {4},
  number = {6},
  pages = {79-84},
  doi = {10.11648/j.ijgg.20160406.16},
  url = {https://doi.org/10.11648/j.ijgg.20160406.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20160406.16},
  abstract = {Parkinson’s disease (PD) is characterized as a disease of the basal ganglia, with a progressive degeneration of dopaminergic neurons located in the substantia nigra (SN) and projecting to the striatum with subsequently loss of the nigrostriatal circuit. The potential for therapeutic use of cell transplantation for cell replacement has received a great deal of interest. Transplantation with embryonic ventral mesencephalon (VM) is a therapeutic approach for sporadic form of PD. We established unilaterally 6-OHDA lesioned rat model of Parkinson’s disease. Motor behavioral impairment was found compared with normal rat. Embryonic VM tissue was isolated from E 14 (embryonic 14 days) rat brain. We characterized the VM tissue and dissociated cultured dopaminergic cells with tyrosine hydroxylase (TH) immune staining before transplantation in lesioned brain. We observed that the axons of dopaminergic neurons from transplanted VM graft circles round at the site of transplantation in normal adult rat brain. In this paper, we discuss the detailed methodologies which are very useful in preclinical research of cell based therapies for Parkinson’s disease.},
 year = {2017}
}

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TY  - JOUR
T1  - Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods
AU  - Biswarup Ghosh
AU  - Angelo Lepore
AU  - George Smith
Y1  - 2017/03/22
PY  - 2017
N1  - https://doi.org/10.11648/j.ijgg.20160406.16
DO  - 10.11648/j.ijgg.20160406.16
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 79
EP  - 84
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20160406.16
AB  - Parkinson’s disease (PD) is characterized as a disease of the basal ganglia, with a progressive degeneration of dopaminergic neurons located in the substantia nigra (SN) and projecting to the striatum with subsequently loss of the nigrostriatal circuit. The potential for therapeutic use of cell transplantation for cell replacement has received a great deal of interest. Transplantation with embryonic ventral mesencephalon (VM) is a therapeutic approach for sporadic form of PD. We established unilaterally 6-OHDA lesioned rat model of Parkinson’s disease. Motor behavioral impairment was found compared with normal rat. Embryonic VM tissue was isolated from E 14 (embryonic 14 days) rat brain. We characterized the VM tissue and dissociated cultured dopaminergic cells with tyrosine hydroxylase (TH) immune staining before transplantation in lesioned brain. We observed that the axons of dopaminergic neurons from transplanted VM graft circles round at the site of transplantation in normal adult rat brain. In this paper, we discuss the detailed methodologies which are very useful in preclinical research of cell based therapies for Parkinson’s disease.
VL  - 4
IS  - 6
ER  -

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Author Information

Biswarup Ghosh

Department of Neuroscience, Vickie & Jack Farber Institute for Neuroscience, Thomas Jefferson University, Philadelphia, USA
Angelo Lepore

Department of Neuroscience, Vickie & Jack Farber Institute for Neuroscience, Thomas Jefferson University, Philadelphia, USA
George Smith

Department of Neuronal Rehabilitation, Shriners Hospitals Pediatric Research Center, Temple University, Philadelphia, USA

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Plain Text BibTeX RIS

APA Style

Biswarup Ghosh, Angelo Lepore, George Smith. (2017). Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods. International Journal of Genetics and Genomics, 4(6), 79-84. https://doi.org/10.11648/j.ijgg.20160406.16

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ACS Style

Biswarup Ghosh; Angelo Lepore; George Smith. Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods. Int. J. Genet. Genomics 2017, 4(6), 79-84. doi: 10.11648/j.ijgg.20160406.16

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AMA Style

Biswarup Ghosh, Angelo Lepore, George Smith. Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods. Int J Genet Genomics. 2017;4(6):79-84. doi: 10.11648/j.ijgg.20160406.16

Copy | Download

@article{10.11648/j.ijgg.20160406.16,
  author = {Biswarup Ghosh and Angelo Lepore and George Smith},
  title = {Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods},
  journal = {International Journal of Genetics and Genomics},
  volume = {4},
  number = {6},
  pages = {79-84},
  doi = {10.11648/j.ijgg.20160406.16},
  url = {https://doi.org/10.11648/j.ijgg.20160406.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20160406.16},
  abstract = {Parkinson’s disease (PD) is characterized as a disease of the basal ganglia, with a progressive degeneration of dopaminergic neurons located in the substantia nigra (SN) and projecting to the striatum with subsequently loss of the nigrostriatal circuit. The potential for therapeutic use of cell transplantation for cell replacement has received a great deal of interest. Transplantation with embryonic ventral mesencephalon (VM) is a therapeutic approach for sporadic form of PD. We established unilaterally 6-OHDA lesioned rat model of Parkinson’s disease. Motor behavioral impairment was found compared with normal rat. Embryonic VM tissue was isolated from E 14 (embryonic 14 days) rat brain. We characterized the VM tissue and dissociated cultured dopaminergic cells with tyrosine hydroxylase (TH) immune staining before transplantation in lesioned brain. We observed that the axons of dopaminergic neurons from transplanted VM graft circles round at the site of transplantation in normal adult rat brain. In this paper, we discuss the detailed methodologies which are very useful in preclinical research of cell based therapies for Parkinson’s disease.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Transplantation of Embryonic Ventral Mesencephalic Tissue in 6-OHDA Induced Parkinsonism Rat Brain for Cell Based Therapy: A Perspective of Methods
AU  - Biswarup Ghosh
AU  - Angelo Lepore
AU  - George Smith
Y1  - 2017/03/22
PY  - 2017
N1  - https://doi.org/10.11648/j.ijgg.20160406.16
DO  - 10.11648/j.ijgg.20160406.16
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 79
EP  - 84
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20160406.16
AB  - Parkinson’s disease (PD) is characterized as a disease of the basal ganglia, with a progressive degeneration of dopaminergic neurons located in the substantia nigra (SN) and projecting to the striatum with subsequently loss of the nigrostriatal circuit. The potential for therapeutic use of cell transplantation for cell replacement has received a great deal of interest. Transplantation with embryonic ventral mesencephalon (VM) is a therapeutic approach for sporadic form of PD. We established unilaterally 6-OHDA lesioned rat model of Parkinson’s disease. Motor behavioral impairment was found compared with normal rat. Embryonic VM tissue was isolated from E 14 (embryonic 14 days) rat brain. We characterized the VM tissue and dissociated cultured dopaminergic cells with tyrosine hydroxylase (TH) immune staining before transplantation in lesioned brain. We observed that the axons of dopaminergic neurons from transplanted VM graft circles round at the site of transplantation in normal adult rat brain. In this paper, we discuss the detailed methodologies which are very useful in preclinical research of cell based therapies for Parkinson’s disease.
VL  - 4
IS  - 6
ER  -

Copy | Download