In order to advocate for informed management decisions with regards to endangered species, we assessed genetic diversity and genetic structure in 40 individuals of six natural populations of the shrub Syncepalum dulcificum (SCHUMACH & THONN.) DANIELL growing in South Western Nigeria. Twelve (12) Random Amplified Polymorphic DNA (RAPD) primers were tested on total genomic DNA extracted from silica gel dried leaves. Bands were then scored for reproducibility and scoring error calculated. Several genetic diversity parameters were then tested using the POPGENE v1.32 software. Five (5) primers produced 227 reproducible and clear RAPD bands of which 47 were polymorphic (20.7%). The percentage of polymorphic loci (PPI) within populations ranged from 36% to 68%. Nei’s gene diversity among population (Hs) was 0.03, while at species level (Ht) was 0.18. The coefficient of gene differentiation (Gst) among populations was estimated to be 0.83 with a gene flow rate (Nm) of 2.49 showing high genetic diversity within and among populations. The results however indicate a high similarity between the populations as well as close genetic relationship among them. We infer that S. dulcificum in Nigeria does not represent a genetically diverse population and this may be accounted for due the plant’s its breeding system which is mainly autogamous. This study further suggests an in-situ form of conservation be set up as immediate rescue conservation procedure for the plant.
| Published in | International Journal of Genetics and Genomics (Volume 4, Issue 6) |
| DOI | 10.11648/j.ijgg.20160406.11 |
| Page(s) | 45-50 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
DNA Markers, Genetic Diversity, RAPD, Syncepalum dulcificum
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APA Style
Iloh Andrew Chibuzor, Orosun Bukola, Akinyele Olukemi Adejoke, Onyenekwe Paul Chidozie. (2017). Genetic Assessment of the Shrub Syncepalum dulcificum (SCHUMACH & THONN.) Daniell in Nigeria Using the Randomly Amplified Polymorphic DNA (RAPD). International Journal of Genetics and Genomics, 4(6), 45-50. https://doi.org/10.11648/j.ijgg.20160406.11
ACS Style
Iloh Andrew Chibuzor; Orosun Bukola; Akinyele Olukemi Adejoke; Onyenekwe Paul Chidozie. Genetic Assessment of the Shrub Syncepalum dulcificum (SCHUMACH & THONN.) Daniell in Nigeria Using the Randomly Amplified Polymorphic DNA (RAPD). Int. J. Genet. Genomics 2017, 4(6), 45-50. doi: 10.11648/j.ijgg.20160406.11
AMA Style
Iloh Andrew Chibuzor, Orosun Bukola, Akinyele Olukemi Adejoke, Onyenekwe Paul Chidozie. Genetic Assessment of the Shrub Syncepalum dulcificum (SCHUMACH & THONN.) Daniell in Nigeria Using the Randomly Amplified Polymorphic DNA (RAPD). Int J Genet Genomics. 2017;4(6):45-50. doi: 10.11648/j.ijgg.20160406.11
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Download@article{10.11648/j.ijgg.20160406.11,
author = {Iloh Andrew Chibuzor and Orosun Bukola and Akinyele Olukemi Adejoke and Onyenekwe Paul Chidozie},
title = {Genetic Assessment of the Shrub Syncepalum dulcificum (SCHUMACH & THONN.) Daniell in Nigeria Using the Randomly Amplified Polymorphic DNA (RAPD)},
journal = {International Journal of Genetics and Genomics},
volume = {4},
number = {6},
pages = {45-50},
doi = {10.11648/j.ijgg.20160406.11},
url = {https://doi.org/10.11648/j.ijgg.20160406.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20160406.11},
abstract = {In order to advocate for informed management decisions with regards to endangered species, we assessed genetic diversity and genetic structure in 40 individuals of six natural populations of the shrub Syncepalum dulcificum (SCHUMACH & THONN.) DANIELL growing in South Western Nigeria. Twelve (12) Random Amplified Polymorphic DNA (RAPD) primers were tested on total genomic DNA extracted from silica gel dried leaves. Bands were then scored for reproducibility and scoring error calculated. Several genetic diversity parameters were then tested using the POPGENE v1.32 software. Five (5) primers produced 227 reproducible and clear RAPD bands of which 47 were polymorphic (20.7%). The percentage of polymorphic loci (PPI) within populations ranged from 36% to 68%. Nei’s gene diversity among population (Hs) was 0.03, while at species level (Ht) was 0.18. The coefficient of gene differentiation (Gst) among populations was estimated to be 0.83 with a gene flow rate (Nm) of 2.49 showing high genetic diversity within and among populations. The results however indicate a high similarity between the populations as well as close genetic relationship among them. We infer that S. dulcificum in Nigeria does not represent a genetically diverse population and this may be accounted for due the plant’s its breeding system which is mainly autogamous. This study further suggests an in-situ form of conservation be set up as immediate rescue conservation procedure for the plant.},
year = {2017}
}
TY - JOUR T1 - Genetic Assessment of the Shrub Syncepalum dulcificum (SCHUMACH & THONN.) Daniell in Nigeria Using the Randomly Amplified Polymorphic DNA (RAPD) AU - Iloh Andrew Chibuzor AU - Orosun Bukola AU - Akinyele Olukemi Adejoke AU - Onyenekwe Paul Chidozie Y1 - 2017/01/24 PY - 2017 N1 - https://doi.org/10.11648/j.ijgg.20160406.11 DO - 10.11648/j.ijgg.20160406.11 T2 - International Journal of Genetics and Genomics JF - International Journal of Genetics and Genomics JO - International Journal of Genetics and Genomics SP - 45 EP - 50 PB - Science Publishing Group SN - 2376-7359 UR - https://doi.org/10.11648/j.ijgg.20160406.11 AB - In order to advocate for informed management decisions with regards to endangered species, we assessed genetic diversity and genetic structure in 40 individuals of six natural populations of the shrub Syncepalum dulcificum (SCHUMACH & THONN.) DANIELL growing in South Western Nigeria. Twelve (12) Random Amplified Polymorphic DNA (RAPD) primers were tested on total genomic DNA extracted from silica gel dried leaves. Bands were then scored for reproducibility and scoring error calculated. Several genetic diversity parameters were then tested using the POPGENE v1.32 software. Five (5) primers produced 227 reproducible and clear RAPD bands of which 47 were polymorphic (20.7%). The percentage of polymorphic loci (PPI) within populations ranged from 36% to 68%. Nei’s gene diversity among population (Hs) was 0.03, while at species level (Ht) was 0.18. The coefficient of gene differentiation (Gst) among populations was estimated to be 0.83 with a gene flow rate (Nm) of 2.49 showing high genetic diversity within and among populations. The results however indicate a high similarity between the populations as well as close genetic relationship among them. We infer that S. dulcificum in Nigeria does not represent a genetically diverse population and this may be accounted for due the plant’s its breeding system which is mainly autogamous. This study further suggests an in-situ form of conservation be set up as immediate rescue conservation procedure for the plant. VL - 4 IS - 6 ER -
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