Escherichia coli sequence type 131 (ST131) is a pandemic clone causing predominantly community and hospital-onset antimicrobial-resistant infections. Recently, H30-Rx subclone of E. coli has been disseminated among ST131 strains in combination with beta lactamase subtype CTX-15that may play a role in the prevalence of antibiotic resistance among E. coli strains that cause extra-intestinal infections. This study has been done to investigate the virulence factors of ST131 with their subclones: H30-Rx and non H30-Rx strains. A hundred E. coli isolates which were isolated from different clinical samples were collected from K.R hospital, Mysore. Polymerase chain reaction (PCR) method was used to investigate 19 of the virulence factors among E. coli stains. Out of 100 E. coli strains, 87 of the isolates belonged to ST131, 24 isolates belonged to H30 subclone, and 22/24 (91.7%) belonged to H30-Rx subcolne. ST131 strains harbored high virulence factors more than non ST131 strains and the differences between them were significant for papC, papEF and fyuA genes. All H30-Rx strains were positive for iutA and fimH, however, 95.5% and 81.8% of H30-Rx strains were positive for fyuA and kpsMII respectively and the differences between H30-Rx and non H30-Rx strains were significant for those genes. There are no more reports about H30 and H30-Rx subclones of ST131 E. coli in India. Further studies should be done to investigate the prevalence of ST131 in different places in India and to highlight the other virulence factors to control the dissemination of ST131 clone.
| Published in | International Journal of Genetics and Genomics (Volume 4, Issue 4) |
| DOI | 10.11648/j.ijgg.20160404.12 |
| Page(s) | 36-39 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
E. coli, ESBL, H30 and H30Rx Subclones, ST131, Virulence Factors
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APA Style
Khadega Yahya Abdullah AL-hetar, Nanjaiah Lakshmidevi. (2016). Virulence Factors of E. coli ST131 and Its H30 and H30Rx Subclones Among Extended-Spectrum Beta-Lactamase Producing Isolates. International Journal of Genetics and Genomics, 4(4), 36-39. https://doi.org/10.11648/j.ijgg.20160404.12
ACS Style
Khadega Yahya Abdullah AL-hetar; Nanjaiah Lakshmidevi. Virulence Factors of E. coli ST131 and Its H30 and H30Rx Subclones Among Extended-Spectrum Beta-Lactamase Producing Isolates. Int. J. Genet. Genomics 2016, 4(4), 36-39. doi: 10.11648/j.ijgg.20160404.12
AMA Style
Khadega Yahya Abdullah AL-hetar, Nanjaiah Lakshmidevi. Virulence Factors of E. coli ST131 and Its H30 and H30Rx Subclones Among Extended-Spectrum Beta-Lactamase Producing Isolates. Int J Genet Genomics. 2016;4(4):36-39. doi: 10.11648/j.ijgg.20160404.12
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Download@article{10.11648/j.ijgg.20160404.12,
author = {Khadega Yahya Abdullah AL-hetar and Nanjaiah Lakshmidevi},
title = {Virulence Factors of E. coli ST131 and Its H30 and H30Rx Subclones Among Extended-Spectrum Beta-Lactamase Producing Isolates},
journal = {International Journal of Genetics and Genomics},
volume = {4},
number = {4},
pages = {36-39},
doi = {10.11648/j.ijgg.20160404.12},
url = {https://doi.org/10.11648/j.ijgg.20160404.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20160404.12},
abstract = {Escherichia coli sequence type 131 (ST131) is a pandemic clone causing predominantly community and hospital-onset antimicrobial-resistant infections. Recently, H30-Rx subclone of E. coli has been disseminated among ST131 strains in combination with beta lactamase subtype CTX-15that may play a role in the prevalence of antibiotic resistance among E. coli strains that cause extra-intestinal infections. This study has been done to investigate the virulence factors of ST131 with their subclones: H30-Rx and non H30-Rx strains. A hundred E. coli isolates which were isolated from different clinical samples were collected from K.R hospital, Mysore. Polymerase chain reaction (PCR) method was used to investigate 19 of the virulence factors among E. coli stains. Out of 100 E. coli strains, 87 of the isolates belonged to ST131, 24 isolates belonged to H30 subclone, and 22/24 (91.7%) belonged to H30-Rx subcolne. ST131 strains harbored high virulence factors more than non ST131 strains and the differences between them were significant for papC, papEF and fyuA genes. All H30-Rx strains were positive for iutA and fimH, however, 95.5% and 81.8% of H30-Rx strains were positive for fyuA and kpsMII respectively and the differences between H30-Rx and non H30-Rx strains were significant for those genes. There are no more reports about H30 and H30-Rx subclones of ST131 E. coli in India. Further studies should be done to investigate the prevalence of ST131 in different places in India and to highlight the other virulence factors to control the dissemination of ST131 clone.},
year = {2016}
}
TY - JOUR T1 - Virulence Factors of E. coli ST131 and Its H30 and H30Rx Subclones Among Extended-Spectrum Beta-Lactamase Producing Isolates AU - Khadega Yahya Abdullah AL-hetar AU - Nanjaiah Lakshmidevi Y1 - 2016/09/18 PY - 2016 N1 - https://doi.org/10.11648/j.ijgg.20160404.12 DO - 10.11648/j.ijgg.20160404.12 T2 - International Journal of Genetics and Genomics JF - International Journal of Genetics and Genomics JO - International Journal of Genetics and Genomics SP - 36 EP - 39 PB - Science Publishing Group SN - 2376-7359 UR - https://doi.org/10.11648/j.ijgg.20160404.12 AB - Escherichia coli sequence type 131 (ST131) is a pandemic clone causing predominantly community and hospital-onset antimicrobial-resistant infections. Recently, H30-Rx subclone of E. coli has been disseminated among ST131 strains in combination with beta lactamase subtype CTX-15that may play a role in the prevalence of antibiotic resistance among E. coli strains that cause extra-intestinal infections. This study has been done to investigate the virulence factors of ST131 with their subclones: H30-Rx and non H30-Rx strains. A hundred E. coli isolates which were isolated from different clinical samples were collected from K.R hospital, Mysore. Polymerase chain reaction (PCR) method was used to investigate 19 of the virulence factors among E. coli stains. Out of 100 E. coli strains, 87 of the isolates belonged to ST131, 24 isolates belonged to H30 subclone, and 22/24 (91.7%) belonged to H30-Rx subcolne. ST131 strains harbored high virulence factors more than non ST131 strains and the differences between them were significant for papC, papEF and fyuA genes. All H30-Rx strains were positive for iutA and fimH, however, 95.5% and 81.8% of H30-Rx strains were positive for fyuA and kpsMII respectively and the differences between H30-Rx and non H30-Rx strains were significant for those genes. There are no more reports about H30 and H30-Rx subclones of ST131 E. coli in India. Further studies should be done to investigate the prevalence of ST131 in different places in India and to highlight the other virulence factors to control the dissemination of ST131 clone. VL - 4 IS - 4 ER -
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